| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,988,951 | A→G | 36.3% | intergenic (+189/‑357) | kefB‑III → / → PP_mr64 | glutathione‑regulated potassium/H+ antiporter/C4 ncRNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,988,951 | 0 | A | G | 36.3% | 78.1 / 51.1 | 58 | intergenic (+189/‑357) | kefB‑III/PP_mr64 | glutathione‑regulated potassium/H+ antiporter/C4 ncRNA |
| Reads supporting (aligned to +/- strand): ref base A (21/16); new base G (9/12); total (30/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.38e-01 | |||||||||||
GGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCC > NC_002947/5988807‑5989096 | gagcgcCGCCCGCGCGGCGCTTCGCGGGTAACCCCGCTCCTACGTTTGTTTCGGCCCCATGTTTCCTGCGGGATTTGCGCGCGA‑‑‑CCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCagga < 1:529771/148‑1 (MQ=2) ggCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACaggag < 2:363920/150‑1 (MQ=255) ggCGCCGCCCGCGCGGCGCTTCGCGCGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGA‑‑‑CCGCCTTGGGGCATGGCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCaggag < 1:57143/150‑1 (MQ=14) gcgcCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGTCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACaggagg < 1:504982/150‑1 (MQ=255) gcgcCGCCCCCGCGGCACTTCGCGGGAAAACCCGCCCCTACGTTTGTTTCGTGCCAATTATCCCTGCGGCATTTGCGCGCGA‑‑‑CCGCCTTGGGGCATGTCTAGATATCGCGGCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCaggagg < 2:570001/150‑1 (MQ=2) cgccggcggcgCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTgg < 1:93247/145‑1 (MQ=255) cccgcGCGGCGCTTCGCGGGTAAACCCGCTCCTACGGTTGTGTGAGGCCAGTTATTCCTGTGGGACGCGCAGGCGAAATTTACCCCAGCGAGCG‑‑‑CGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACaggagatcggaa > 1:779328/1‑143 (MQ=16) cggcgCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGA‑‑‑CCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGccgaaa < 2:93414/150‑5 (MQ=9) ttCGCGGGTAACCCCGCTCCTACGTTCGTTACCGGCAACTTAGGGCTGGGGGACGCGCACGCGA‑‑‑GCCCCATGTCCCGCGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaaac < 1:775588/150‑1 (MQ=21) gcgGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGAGTTGCGCGCGA‑‑‑CCGCCTTGGGGCATGTCTAGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAg > 2:47604/1‑150 (MQ=9) cgGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTACTGTGGGACCCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGAGGCTCGCGGCTGGCACGGGAGGAATTGACCAGaaaaaaaccgag > 2:324207/1‑146 (MQ=25) gggTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGAtt > 1:415183/1‑131 (MQ=255) gggTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGAtt < 2:415183/131‑1 (MQ=255) gggTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaa > 2:654768/1‑143 (MQ=255) gggTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaa < 1:654768/143‑1 (MQ=255) gttAAACCCGCTCCTACGTTTGTTTCGGGCCACATGATTAAGCGGGATTTGCGCGCGA‑‑‑CCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAg < 1:439329/148‑1 (MQ=2) gTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAg > 2:424708/1‑150 (MQ=255) tAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaaac < 1:522436/142‑1 (MQ=255) tAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaaac > 2:522436/1‑142 (MQ=255) tAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGc > 2:455437/1‑150 (MQ=255) aaaCCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTCCGGGCTTTGCGCGCCC‑‑‑CCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCgg < 1:398404/150‑1 (MQ=2) cccGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGt < 1:687689/150‑1 (MQ=255) ccGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGtt > 1:118842/1‑150 (MQ=255) gCTCCTACGTTTTTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGA‑‑‑CCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAc < 2:368434/150‑1 (MQ=2) gCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGA‑‑‑CCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAc < 2:77554/150‑1 (MQ=2) cTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaaac > 1:440426/1‑131 (MQ=255) cTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaaac < 2:440426/131‑1 (MQ=255) cTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAg > 2:400833/1‑135 (MQ=255) cTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAg < 1:400833/135‑1 (MQ=255) tACGTTTGTTTCGGGCAAATTATTCCTGCGGGATTTGCGCGCGA‑‑‑CCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCcgcg > 2:469513/1‑150 (MQ=2) gtttCGGGCCAATTATTCCTGCGGGATTTGCGCGCGA‑‑‑CCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGcgcc > 1:609278/1‑150 (MQ=9) tttCGGGCCAATTATTCCTGCGGGATTTGCGCGCGA‑‑‑CCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGcgccg > 2:436596/1‑150 (MQ=9) gggCCAATTATTCCTGCGGGATTTGCGCGCGA‑‑‑CCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGGCTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCcgcgcg > 1:155630/1‑150 (MQ=2) aCGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATg > 1:674720/1‑150 (MQ=255) cgcgCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATgg < 1:424708/150‑1 (MQ=255) gCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCaaaaa > 2:784424/1‑150 (MQ=255) cTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATc < 2:118842/150‑1 (MQ=255) gcatgtctcgataTCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgat > 1:700693‑M2/7‑90 (MQ=255) gcatgtctcgataTCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgat < 2:700693‑M2/115‑32 (MQ=255) gcACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCAGCGCGGGCGGCGGTCGATCTCATTGGCGCCCAAAAACTATCGCCAg > 2:728886/1‑150 (MQ=255) tagatATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagc > 2:290721‑M2/3‑83 (MQ=255) tCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagc < 1:290721‑M2/93‑11 (MQ=255) gATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGa > 2:345793/1‑150 (MQ=255) gATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGACCAAAAACTTTTGGCAGCCACCTGGa > 2:382074/1‑150 (MQ=21) gcgTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattcca > 1:47604‑M2/1‑75 (MQ=255) gtcgtACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATcc > 2:559292/1‑150 (MQ=255) tACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggagctagatctcacattcgctgaaaatcctccggcaaacacctcaaaattccaacgccg < 1:651744‑M2/150‑82 (MQ=255) aaacaaGGCGGTCGCGCGCGCCTGACATGAGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAAtt > 1:543262/1‑150 (MQ=255) aaacaaGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaa > 1:640484‑M2/1‑66 (MQ=255) aaacaaGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaa < 2:640484‑M2/136‑71 (MQ=255) aacaaGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcccgcgcggcgcttcgc > 2:564724‑M2/1‑66 (MQ=255) aacaaGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcccgcgcggcgcttcgc < 1:564724‑M2/97‑32 (MQ=255) acaaGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTc > 1:390109/1‑150 (MQ=255) cGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACg < 2:390109/150‑1 (MQ=255) gTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGc < 1:94895/113‑1 (MQ=255) gTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGc > 2:94895/1‑113 (MQ=255) cgcgcgcgCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCa < 1:345793/150‑1 (MQ=255) tGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCgccgcc > 2:600915/1‑150 (MQ=255) | GGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑GCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCC > NC_002947/5988807‑5989096 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |