| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,005,077 | G→C | 8.5% | intergenic (+59/‑117) | PP_5259 → / → ydcJ | LysR family transcriptional regulator/metalloprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,005,077 | 0 | G | C | 8.5% | 141.8 / 5.4 | 47 | intergenic (+59/‑117) | PP_5259/ydcJ | LysR family transcriptional regulator/metalloprotein |
| Reads supporting (aligned to +/- strand): ref base G (21/22); new base C (2/2); total (23/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.19e-01 | |||||||||||
ATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGTAAGCGTCCGAACCCAACCAGGAAGCTAGCGATGCCCGCCAACGATTTCGTCAGCCCAGACAGC > NC_002947/6004933‑6005226 | aTTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGTTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGGAGGCGCGGCTTTAGCAGGGAAAg > 1:731310/1‑150 (MQ=255)aTTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAg > 1:770343/1‑150 (MQ=255)aTTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTCAGCAAGGCATTCGGTTTCTACGGAGACCGCGTAGGCGCGGCTTTAGCCGGGAAAg > 2:20559/1‑150 (MQ=255) gTATGCCGAGCATGCGGCAGAGGTGCCAAAGGGGCGGGCGCTGGTGGAATGGATTGGCGCGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGCAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAg < 2:600644/150‑1 (MQ=255) tGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAggg < 1:224015/139‑1 (MQ=255) tGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAggg > 2:224015/1‑139 (MQ=255) gCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCaaaaaa > 1:89756/1‑150 (MQ=255) gCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGCTGCAGGTTCGACGGCCCTTTCCCGGCTAAAGCCGCGCCTACGCGGTCCCCGTAGGAGCGGCTTTAGCCGCGaaga > 2:334545/1‑122 (MQ=14) gCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGCTGCAGGTTCGACGGCCCTTTCCCGGCTAAAGCCGCGCCTACGCGGTCCCCGTAGGAGCGGCTTTAGCCGCGaaga < 1:334545/124‑3 (MQ=14) cAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGaa < 2:481187/150‑1 (MQ=255) ccAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCATACGCGCGGCGTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGacacac > 1:776729/1‑150 (MQ=255) gTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGCCACACTCACtt < 2:715106/150‑1 (MQ=255) ggggCGCTGGAGGAATGGATTGGCGAGCAGTTGCAGGTTTGCCGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACttttt < 2:770343/149‑1 (MQ=255) gggCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACtttttt > 1:333085/1‑150 (MQ=255) ggAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGa < 2:553215/150‑1 (MQ=255) gAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAg < 2:241155/150‑1 (MQ=255) aTGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCTCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCa > 2:495099/1‑150 (MQ=255) ggATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCaaa < 1:299367/150‑1 (MQ=255) tgAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGATAAAGCGGCTCCTACGCAGACCGCGTAGGCGCAACTTTAGCCGGGAAAGGGCCGTCGAGGTATATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCAtt < 2:12937/149‑1 (MQ=255) cGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGAGCGCGTAGGCGCGGCGTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTATCTTGTGAGGAAAGTGCagg > 1:292762/1‑148 (MQ=255) cAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTGGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCa > 1:377516/1‑150 (MQ=255) aGGTGTGAAGGCCTCTTGGCGGATAAAGCCGCTCTTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCGGTCGAGGTTTATCAAAAAAGGAATGACACACTCACTTTTTTTCGTTTGTGAGCAAAGTGCATTCCCAGCTTTCa < 2:331303/150‑1 (MQ=255) gTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATg > 1:611489/1‑150 (MQ=255) tttGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTTCGGTTAAAGTGCATTCCCAGCTTTCATGt < 1:20559/150‑1 (MQ=255) ccgatctcctcGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGTCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGTAAGc < 2:420016/145‑1 (MQ=255) aCGGCCTCTTCGCGGGTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTTGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGTAAgg > 2:595017/1‑149 (MQ=255) gCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGTAAGCGTc > 1:54881/1‑150 (MQ=255) gCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGTAAGCGTc < 2:776729/150‑1 (MQ=255) ccTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAgg > 2:640051/1‑72 (MQ=255) ccTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAgg < 1:640051/72‑1 (MQ=255) ctTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGTAAGCGTCCGaa < 2:333085/150‑1 (MQ=255) ttCGCGGCTAAAGCCGCTCCTACGGGGTCCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGTAAGCgagatcgg > 1:420016/1‑143 (MQ=255) ttCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAg > 2:478011/1‑81 (MQ=255) ttCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAg < 1:478011/81‑1 (MQ=255) ccGCGCCTACGCGGTCCCCGTAGGAGCGGCTTTAGCCGGGAAAGGGCCGTCTAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGTAAGCGTCCGAACCCAACCAGGAAGc > 2:311682/1‑150 (MQ=38) gcgccTACGCGGTCCCCGTAGGAGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGTAAGCGTCCGAACCCAACCAGGAAGCTa > 1:422578/4‑150 (MQ=39) gcgccTACGCGGTCCCCGTAGGAGCGGCTTTAGCCGCGAAGAGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGa < 1:630743/94‑1 (MQ=17) gcgccTACGCGGTCCCCGTAGGAGCGGCTTTAGCCGCGAAGAGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGa > 2:630743/4‑97 (MQ=17) cTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGTAAGCGTCCGAACCCAACCAGGAAGCTAGCGa > 1:426637/1‑150 (MQ=255) aCGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGTAAGCGTCCGAACCCAACCAGGAAGCTAGCGATg > 1:456562/1‑150 (MQ=255) gggCCCGCGTGCGCGCTGCTTCAGGCGGGAAAGGGACGTAGAGGTAGATCAAAAAAGTAATGACACACTCACTTTTTTTCGCTTGTGATCAAAGTGTATGCCCAGGTTTCATGTAAGCGTCCGACCCCAACCAGGAAGCTAGCGATGccc < 2:292762/150‑1 (MQ=255) gagcgGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGACCAATAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTAAAGTCCGCGTGGGAGACCGACCATGGGTCTAGCGATGCCCGCCAACGATTTc < 1:698857/148‑1 (MQ=255) gcgGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGTAAGCGTCCGAACCCAACCAGGAAGCTAGCGATGCCCGCCAACGATTTCGt > 2:831/1‑150 (MQ=255) tttAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGTAAGCGTCCGAACCCAACCAGGAAGCTAGCGATGCCCGCCAACGATTTCGTCAGcc < 2:377516/150‑1 (MQ=255) tttAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGTAAGCGTCCGAACCCAACCAGGAAGCTAGCGATGCCCGCCAACGATTTCGTCAGcc < 2:731310/150‑1 (MQ=255) ttgagacGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGTAAGCGTCCGAACCCAACCAGGAAGCTAGCGATGCCCGCCAACGATTTCGTCAGcc < 2:270134/144‑1 (MQ=255) gCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGTAAGCGTCCGAACCCAACCAGGAAGCTAGCGATGCCCGCCAACGATTTCGTCAGCCCAGa < 2:611489/150‑1 (MQ=255) ggAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGTAAGCGTCCGAACCCAACCAGGAAGCTAGCGATGCCCGCCAACGATTTCGTCAGCCCAGACAGc < 1:746993/150‑1 (MQ=255) | ATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGTAAGCGTCCGAACCCAACCAGGAAGCTAGCGATGCCCGCCAACGATTTCGTCAGCCCAGACAGC > NC_002947/6004933‑6005226 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |