Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,015,757 G→C 11.1% H22D (CAT→GAT)  PP_5266 ← acetyl‑CoA hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,015,7570GC11.1% 106.4 / 5.8 36H22D (CAT→GAT) PP_5266acetyl‑CoA hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base G (15/17);  new base C (2/2);  total (17/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

GCGCCGCTGCAGGCCATCGCCCAGTGGCGGGCGGCCGAGGGAAAGTGCGGTATAGATGGTCAGGCGCCGTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGCTCAAGCGGGTTGGTTGCCTAGGGCAAACTCTGGTCAAC  >  NC_002947/6015608‑6015902
                                                                                                                                                     |                                                                                                                                                 
gcgcCGCTGCAGGCCATCGCCCAGTGGCGGGCGGCCGAGGGAAAGTGCGGTATAGATGGTCAGGCGCCGTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCatatg                                                                                                                                                   >  2:450070/1‑150 (MQ=255)
           ggCCATCGCCCAGTGGCGGGCGGCCGAGGGAAAGTGCGGTATAGATGGTCAGGCGCCGTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTg                                                                                                                                        <  2:417359/150‑1 (MQ=255)
              cATCGCCCAGTGGCGGGCGGCCGAGGGAAAGTGCGGTATAGATGGTCAGGCGCCGTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCa                                                                                                                                     >  1:396464/1‑150 (MQ=255)
                                     aGGGAAAGTGCGGTATAGATGGTCAGGCGCCGTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGAc                                                                                                                        <  2:547635/140‑1 (MQ=255)
                                     aGGGAAAGTGCGGTATAGATGGTCAGGCGCCGTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGAc                                                                                                                        >  1:547635/1‑140 (MQ=255)
                                       ggAAAGTGCGGTATAGATGGTCAGGCGCCGTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGc                                                                                                            <  1:594326/150‑1 (MQ=255)
                                                 gTATAGATGGTCAGGCGCCGTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATg                                                                                                  <  2:704177/150‑1 (MQ=255)
                                                 gTATAGATGGTCAGGCGCCGTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATg                                                                                                  <  1:778752/150‑1 (MQ=255)
                                                       aTGGTCAGTCGCCGTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCatatgg                                                                                                                                                  >  2:595575/1‑96 (MQ=255)
                                                       aTGGTCAGGCGCCGTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCatatgg                                                                                                                                                  <  1:595575/96‑1 (MQ=255)
                                                              ggCGCCGTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCa                                                                                     >  1:99249/1‑150 (MQ=255)
                                                               gcgcCGTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCAt                                                                                    <  2:99249/150‑1 (MQ=255)
                                                                  ccGTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCg                                                                                                                                 >  1:144677/1‑102 (MQ=255)
                                                                  ccGTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCg                                                                                                                                 <  2:144677/102‑1 (MQ=255)
                                                                   cGTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATgggg                                                                                >  1:527529/1‑150 (MQ=255)
                                                                    gTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGc                                                                               <  1:450070/150‑1 (MQ=255)
                                                                       cGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGcctc                                                                            <  1:164486/150‑1 (MQ=255)
                                                                         ggCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAATAGCGGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGTGcctcct                                                                          >  2:419431/1‑150 (MQ=255)
                                                                            aGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGaa                                                                       >  1:30941/1‑150 (MQ=255)
                                                                                              tAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAggg                                                     <  1:728233/150‑1 (MQ=255)
                                                                                                          aCGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAg                                         >  1:672457/1‑150 (MQ=255)
                                                                                                            gAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACAt                                            <  2:139947/145‑1 (MQ=255)
                                                                                                            gAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACAt                                            >  1:139947/1‑145 (MQ=255)
                                                                                                            gAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGCt                                       >  1:617711/1‑150 (MQ=255)
                                                                                                            gAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGCt                                       >  1:228631/1‑150 (MQ=255)
                                                                                                                     gggTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGCTCAAGCGggt                              <  2:527529/150‑1 (MQ=255)
                                                                                                                            cccAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGCTCAAGCGGGTTGGTTGc                       >  1:224967/1‑150 (MQ=255)
                                                                                                                                 gCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGCTCAAGCGGGTTGGTTGCCTAgg                  <  2:30941/150‑1 (MQ=255)
                                                                                                                                  cccAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGCTCAAGCGGGTTGGTTGCCTAggg                 <  2:672457/150‑1 (MQ=255)
                                                                                                                                    cAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGCTCAAGCGGGTTGGTTGCCTAGGGCa               >  2:558319/1‑150 (MQ=255)
                                                                                                                                      aaGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGCTCAAGCGGGTTGGTTGCCTAg                   <  1:512209/144‑1 (MQ=255)
                                                                                                                                      aaGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGCTCAAGCGGGTTGGTTGCCTAg                   >  2:512209/1‑144 (MQ=255)
                                                                                                                                       aggcaggcCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGCTCAAGCGGGTTGGTTGCCTAGGGCAAAc            <  1:558319/150‑1 (MQ=255)
                                                                                                                                          ccagcCCATATCCATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGaa                                                                       >  2:581871/4‑88 (MQ=255)
                                                                                                                                          ccagcCCATATCCATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGaa                                                                       <  1:581871/85‑1 (MQ=255)
                                                                                                                                           cagcCCATATCCATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGt                                                                                        <  2:3424/68‑1 (MQ=38)
                                                                                                                                           cagcCCATATCCATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGt                                                                                        >  1:3424/3‑70 (MQ=38)
                                                                                                                                           aggcCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGCTCAAGCGGGTTGGTTGCCTAGGGCCACCTCTg        <  2:396464/150‑1 (MQ=255)
                                                                                                                                               ccATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGCTCAAGCGGGTTGGTTGCCTAGGGCAAACTCTGGTCa    >  1:433517/1‑150 (MQ=255)
                                                                                                                                                 atatggatatggGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAGCATCCGTGGGTTCAGGATGTGTACATTAGCTCAAGCGGGTTGGTTGCCTAGGGCAAACTCTGGTCAAc  >  2:89539/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
GCGCCGCTGCAGGCCATCGCCCAGTGGCGGGCGGCCGAGGGAAAGTGCGGTATAGATGGTCAGGCGCCGTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGCTCAAGCGGGTTGGTTGCCTAGGGCAAACTCTGGTCAAC  >  NC_002947/6015608‑6015902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑