Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,052,930 A→C 14.7% L198R (CTG→CGG) ‡ PP_5305 ← epimerase/dehydratase‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,052,9300AC14.7% 89.1 / 12.1 41L198R (CTG→CGG) ‡PP_5305epimerase/dehydratase‑like protein
Reads supporting (aligned to +/- strand):  ref base A (21/14);  new base C (3/3);  total (24/17)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.08e-01

ATCGGACCATTCGGTAACCCCCATGTAGGCCCGCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAGCAACCACTCGCGCCCTGGGCCATAGATACCGGTCAGGCGGACCACGCTCGCCGGGATGC  >  NC_002947/6052791‑6053074
                                                                                                                                           |                                                                                                                                                
aTCGGACCATTCGGTAACCCCCATGTAGGCCCGCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTggcggc                                                                                                                                        >  2:257531/1‑150 (MQ=255)
      ccATTCGGTAACCCCCATGTAGGCCCGCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTc                                                                                                                                  <  2:121580/150‑1 (MQ=255)
        aTTCGGTAACCCCCATGTAGGCCCGCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCgg                                                                                                                                >  2:284386/1‑150 (MQ=255)
                  ccccATGTAGGCCCGCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCCTGCTggcgtttctg                                                                                                                                    <  2:717752/136‑7 (MQ=255)
                  ccccATGTAGGCCCGCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCg                                                                                                                      >  2:124609/1‑150 (MQ=255)
                  ccccATGTAGGCCCGCAGCCAGGCAACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCCTGCTggcgtttctg                                                                                                                                    >  1:717752/1‑130 (MQ=255)
                   cccATGTAGGCCCGCAGCCAGGCCACCACATCGGAAAGCCGCGCCGAGTTGTCAGCAACGCCAATGTAGCAGGCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCgg                                                                                                                     >  1:15111/1‑150 (MQ=255)
                    ccATGTAGGCCCGCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAgg                                                                                                                                                <  2:616767/122‑1 (MQ=255)
                    ccATGTAGGCCCGCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAgg                                                                                                                                                >  1:616767/1‑122 (MQ=255)
                      aTGTAGGCCCGCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTg                                                                                                                  <  2:383322/150‑1 (MQ=255)
                               cGCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAgc                                                                                                         >  1:703447/1‑150 (MQ=255)
                                gCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAgcg                                                                                                        >  2:258680/1‑150 (MQ=255)
                                  aGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGCAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAgcggc                                                                                                      <  1:258680/150‑1 (MQ=255)
                                    ccAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCCTGCTggcgtttc                                                                                                                                      >  2:587130/1‑112 (MQ=255)
                                    ccAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCCTGCTggcgtttc                                                                                                                                      <  1:587130/116‑5 (MQ=255)
                                      aGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGct                                                                                                  >  2:309899/1‑150 (MQ=255)
                                          caccacATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATTGGCCTGCAGCAGAAACGCCAGCCTGCTggcgttt                                                                                                                                       >  1:518383/1‑106 (MQ=255)
                                          caccacATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATTGGCCTGCAGCAGAAACGCCAGCCTGCTggcgttt                                                                                                                                       <  2:518383/109‑4 (MQ=255)
                                          caccacATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGctcctc                                                                                              <  1:268824/150‑1 (MQ=255)
                                                             gcCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGa                                                                           <  1:309899/150‑1 (MQ=255)
                                                             gcCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGa                                                                           <  1:284386/150‑1 (MQ=255)
                                                              ccGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGAc                                                                          <  1:124609/150‑1 (MQ=255)
                                                                      tcatcaACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTgg                                                                  >  2:770876/1‑150 (MQ=255)
                                                                         tcaACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTc                                                               >  2:331370/1‑150 (MQ=255)
                                                                            aCGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAGc                                                            <  2:539720/150‑1 (MQ=255)
                                                                              gCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAGCaa                                                          >  2:129350/1‑150 (MQ=255)
                                                                              gCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAGCaa                                                          >  1:632808/1‑150 (MQ=255)
                                                                                      aGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAGCAAc                                                         <  1:506661/143‑1 (MQ=255)
                                                                                      aGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAGCAAc                                                         >  2:506661/1‑143 (MQ=255)
                                                                                                          ccATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAGCAACCACTCGCGCCCTGGGCAATAGATACCg                              >  1:331114/1‑150 (MQ=255)
                                                                                                           cATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAGCAACCACTCGCGCCCTGGGCCATAGATACCgg                             >  1:94806/1‑150 (MQ=255)
                                                                                                              cAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCaa                                                                                         >  2:262227/1‑87 (MQ=255)
                                                                                                              cAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCaa                                                                                         <  1:262227/87‑1 (MQ=255)
                                                                                                                  aTCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAGCAACCACTCGCGCCCTGGGCCATAGATAc                                <  1:702469/140‑1 (MQ=255)
                                                                                                                  aTCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAGCAACCACTCGCGCCCTGGGCCATAGATAc                                >  2:702469/1‑140 (MQ=255)
                                                                                                                      gCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGCCGCACCTGGCTCAGCAACCACTCGCGCCCTGGGCCATAGATACCGGTCAGGCGGAcc                  <  1:257531/150‑1 (MQ=255)
                                                                                                                           cagcagAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAGCAACCACTCGCGCCCTGGGCCATAGATACCGGTCAGGCGGACCACGCt             >  1:112705/1‑150 (MQ=255)
                                                                                                                                     gCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAGCAACCACTCGCGCCCTGGGCCATAGATACCGGTCAGGCGGACCACGCTCGCCGGGATg   >  1:65878/1‑150 (MQ=255)
                                                                                                                                      ccAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTaa                                                                                 >  1:600343/1‑71 (MQ=255)
                                                                                                                                      ccAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTaa                                                                                 <  2:600343/71‑1 (MQ=255)
                                                                                                                                      ccAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAGCAACCACTCGCGCCCTGGGCCATAGATACCGGTCAGGCGGACCACGCTCGCCGGGATGc  >  2:338901/1‑150 (MQ=255)
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ATCGGACCATTCGGTAACCCCCATGTAGGCCCGCAGCCAGGCCACCACATCGGCCAGCGGCGCCGGGTCGTCATCAACGCCAATGTAGCAGTCGTCCAGAGCCTTGCCATCAGCATCGGCCTGCAGCAGAAACGCCAGCAGGCTGGCGGCATCTTCGGCATGAATGCGGTTGCCATACAGCGGCGGCTCCTCGGCAACGCGGTAACCCTGACGCACCTGGCTCAGCAACCACTCGCGCCCTGGGCCATAGATACCGGTCAGGCGGACCACGCTCGCCGGGATGC  >  NC_002947/6052791‑6053074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑