Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,070,834	A→T	6.3%	intergenic (‑134/+29)	PP_5324 ← / ← phoU	two‑component system response regulator/phosphate transport‑associated protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,070,834	0	A	T	6.3%	190.8 / 4.3	63	intergenic (‑134/+29)	PP_5324/phoU	two‑component system response regulator/phosphate transport‑associated protein
Reads supporting (aligned to +/- strand):  ref base A (30/29);  new base T (2/2);  total (32/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.87e-01

ATTCACTTTACTCATCGATACTCCTTTTGAATCCCGACTAGCCTACTGCCAATGATGGCCACTTGCTACAACCTGGTCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTCGCCGTTGGCGACAGCAGTGCCCTGCACTTCTGCCGTCATGCGCTTGAGGCCCATGTGGCGCACGTCGGTGCCGCGCACC  >  NC_002947/6070686‑6070980                                                                                                                                                     |                                                                                                                                                   aTTCACTTTACTCATCGATACTCCTTTTGAATCCCGACTAGCCTACTGCCAATGATGGCCACTTGCTACAACCTGGTCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAg                                                                                                                                                   >  1:59348/1‑150 (MQ=255)  ttCACTTTACTCATCGATACTCCTTTTGAATCCCGACTAGCCTACTGCCAATGATGGCCACTTGCTACAACCTGGTCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAgg                                                                                                                                                  >  1:124891/1‑150 (MQ=255)                  aTACTCCTTTTGAATCCCGACTAGCCTACTGCCAATGATGGCCACTTGCTACAACCTGGTCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTc                                                                                                                                  <  2:694146/150‑1 (MQ=255)                    aCTCCTTTTGAATCCCGACTAGCCTACTGCCAATGATGGCCACTTGCTACAACCTGGTCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCgg                                                                                                                                <  2:124891/150‑1 (MQ=255)                    aCTCCTTTTGAATCCCGACTAGCCTACTGCCAATGATGGCCACTTGCTACAACCTGGTCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCgg                                                                                                                                <  1:144712/150‑1 (MQ=255)                      tCCTTTTGAATCCCGACTAGCCTACTGCCAATGATGGCCACTTGCTACAACCTGGTCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGa                                                                                                                              >  2:284124/1‑150 (MQ=255)                             gAATCCCGACTAGCCTACTGCCAATGATGGCCACTTGCTACAACCTGGTCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGa                                                                                                                              <  1:784235/143‑1 (MQ=255)                             gAATCCCGACTAGCCTACTGCCAATGATGGCCACTTGCTACAACCTGGTCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGa                                                                                                                              >  2:784235/1‑143 (MQ=255)                                tCCCGACTAGCCTACTGCCAATGATGTCCACTTGCTACAACCTGGTCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACcttac                                                                                                                    >  2:381233/1‑150 (MQ=255)                                tCCCGACTAGCCTACTGCCAATGATGTCCACTTGCTACAACCTGGTCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACcttac                                                                                                                    <  1:381233/150‑1 (MQ=255)                                tCCCGACTAGCCTACTGCCAATGATGGCCACTTGCTACAACCTGGTCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAGAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACcttac                                                                                                                    >  1:485319/1‑150 (MQ=255)                                tCCCGACTAGCCTACTGCCAATGATGGCCACTTGCTACAACCTGGTAACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACcttcc                                                                                                                    <  2:77343/150‑3 (MQ=255)                                 cccGACTAGCCTACTGCCAATGATGGCCACTTGCTACAACCTGGTCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACcttact                                                                                                                   <  1:284124/150‑1 (MQ=255)                                     acttgCCTACTGCCAATGATGGCCACTTGCTACAACCTGGTCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATc                                                                                                               <  1:145445/146‑1 (MQ=255)                                       tAGCCTACTGCCAATGATGGCCACTTGCTACAACCTGGTCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGt                                                                                                             <  1:438146/150‑1 (MQ=255)                                               tGCCAATGATGGCCACTTGCTACAACCTGGTCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCa                                                                                                     >  1:207598/1‑150 (MQ=255)                                                  cAATGATGGCCACTTGCTACAACCTGGTCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCAccc                                                                                                  >  2:742474/1‑150 (MQ=255)                                                  cAATGATGGCCACTTGCTACAACCTGGTCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCAcac                                                                                                  >  2:392813/1‑148 (MQ=255)                                                   aaTGATGGCCACTTGCTACAACCTGGTCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCTTGGCCGGGCGt                                                                                                                                          <  1:313547/109‑1 (MQ=255)                                                   aaTGATGGCCACTTGCTACAACCTGGTCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCTTGGCCGGGCGt                                                                                                                                          >  2:313547/1‑109 (MQ=255)                                                      gatgGCCACTTGCTACCTTCTGGCCACCACGTGTTTTCTGGTCTTCTTTCGCTGTGGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGaa                                                                                              <  2:485319/150‑1 (MQ=255)                                                           ccACTTGCTACAACCTGGTCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGa                                                                                                         >  2:621952/1‑134 (MQ=255)                                                           ccACTTGCTACAACCTGGTCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGa                                                                                                         <  1:621952/134‑1 (MQ=255)                                                           ccACTTGCTACAACCTGGTCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTa                                                                                         >  2:524411/1‑150 (MQ=255)                                                              cTTGCTACAACCTGGTCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGcc                                                                                      >  1:236206/1‑150 (MQ=255)                                                                  cTACAACCTGGTCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCtttt                                                                                  >  1:184685/1‑150 (MQ=255)                                                                   tACAACCTGGTCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTc                                                                                 >  2:697342/1‑150 (MQ=255)                                                                   tACAACCTGGTCACGCCGGGACTTCTTCACACCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTc                                                                                 <  2:56822/150‑1 (MQ=255)                                                                        ccTGGTCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTCGCCGt                                                                            >  2:137266/1‑150 (MQ=255)                                                                             tCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGCTGCTCGGGAGCAAc                                                                                                                         <  2:629081/100‑1 (MQ=255)                                                                             tCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAAc                                                                                                                         >  1:629081/1‑100 (MQ=255)                                                                             tCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACcttact                                                                                                                   >  2:406438/1‑106 (MQ=255)                                                                             tCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACcttact                                                                                                                   <  1:406438/106‑1 (MQ=255)                                                                               aCGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGa                                                                                                                              <  2:105984/93‑1 (MQ=255)                                                                               aCGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGa                                                                                                                              >  1:105984/1‑93 (MQ=255)                                                                               aCGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCAGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATGGTCAGATTCACACGGAAAATTAGCCTTTTTGCCGTTGgagaa                                                                     >  2:316927/1‑149 (MQ=255)                                                                                    gggACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCa                                                                                                           <  2:735432/107‑1 (MQ=255)                                                                                    gggACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCa                                                                                                           >  1:735432/1‑107 (MQ=255)                                                                                     ggACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTCGCCGTTGGCGACAGCAGt                                                               <  1:524411/150‑1 (MQ=255)                                                                                        cttcttCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTCGCCGTTGGCGACAGCAGTGcc                                                            <  1:137266/150‑1 (MQ=255)                                                                                         ttcttcACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTCGCCGTTGGCGACAGCAGTGccc                                                           >  1:139468/1‑150 (MQ=255)                                                                                               ccaTCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTCGCCGTTGGCGAca                                                                    >  1:626371/2‑135 (MQ=255)                                                                                               acaTCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTCGCCGTTGGCGAca                                                                    <  2:626371/135‑1 (MQ=255)                                                                                                caTCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTCGCCGTTGGCGACAGCAGTGCCCTGCACtt                                                    <  1:772302/150‑1 (MQ=255)                                                                                                     ttCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAAcc                                                                                                                        >  1:522841/1‑77 (MQ=255)                                                                                                     ttCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAAcc                                                                                                                        <  2:522841/77‑1 (MQ=255)                                                                                                     ttCGCGGGTGCCTGCCGGCTCTCGACCGCAAAAGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTACCCTTTTCGCCGTTGGCGACAGCAGTGCCCTGCACTCCTgac                                               >  1:55249/1‑148 (MQ=255)                                                                                                       cgcgGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTCGCCGTTGGCGACAGCAGTGCCCTGCACTTCTGCCGt                                             <  2:236206/150‑1 (MQ=255)                                                                                                          gggTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTCGCCGTTGGCGACAGCAGTGCCCTGCACTTCTGCCGTCAt                                          <  2:392161/150‑1 (MQ=255)                                                                                                                       ctctCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTCGCCGTTGGCGACAGCAGTGCCCTGCACTTCTGCCGTCATGCGCTTGAGGccc                             >  2:9841/1‑150 (MQ=255)                                                                                                                            gACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTCGCCGTTGGCGACAGCAGTGCCCTGCACTTCTGCCGTCATGCGCTTGAGACCCAtgtg                        >  2:476872/1‑150 (MQ=255)                                                                                                                                   aaCGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTCGCCGTTGGCGACAGCAGTGCCCTGCACTTCTGCCGTCATGCGCTTGAGGCCCATGTGGCGCACg                 >  1:189793/1‑150 (MQ=255)                                                                                                                                      gAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTCGCCGTTGGCGACAGCAGTGCCCTGCACTTCTGCCGTCATGCGCTTGAGGCCCATGTGGCGCAc                  <  2:348325/146‑1 (MQ=255)                                                                                                                                      gAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTCGCCGTTGGCGACAGCAGTGCCCTGCACTTCTGCCGTCATGCGCTTGAGGCCCATGTGGCGCAc                  >  1:348325/1‑146 (MQ=255)                                                                                                                                      gAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTCGCCGTTGGCGACAGCAGTGCCCTGCACTTCTGCCGTCATGCGCTTGAGGCCCATGTGGCGCACGTCg              <  2:207598/150‑1 (MQ=255)                                                                                                                                         aaCGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGACAGATTCACCCGGAACATTAGCCTTTTCGCCGTTGGCGACAGCAGTGCCCTGCACTTCTGCCGTCATGCGCTTGAGGCCCATGTGGCGCACGTCGGTg           <  2:189793/150‑1 (MQ=255)                                                                                                                                          aCGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTCGCCGTTGGCGACAGCAGTGCCCTGCACTTCTGCCGTCATGCGCTTGAGGCCCATGTGGCGCACGTCGGTGc          <  1:557913/150‑1 (MQ=255)                                                                                                                                           cGCCCGGCCTTGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTCGCCGTTGGCGACAGCAGTGCCCTGCACTTCTGCCGTCATGCGCTTGAGGCCCAtgtg                        >  1:310084/1‑135 (MQ=255)                                                                                                                                           cGCCCGGCCTTGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTCGCCGTTGGCGACAGCAGTGCCCTGCACTTCTGCCGTCATGCGCTTGAGGCCCAtgtg                        <  2:310084/135‑1 (MQ=255)                                                                                                                                            gCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTCGCCGTTGGCGACAGCAGTGCCCTGCACTTCTGCCGTCATGCGCTTGAGGCCCAt                           <  2:518481/131‑1 (MQ=255)                                                                                                                                            gCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTCGCCGTTGGCGACAGCAGTGCCCTGCACTTCTGCCGTCATGCGCTTGAGGCCCAt                           >  1:518481/1‑131 (MQ=255)                                                                                                                                            gCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTCGCCGTTGGCGACAGCAGTGCCCTGCACTTCTGCCGTCATGCGCTTGAGGCCCATGTGGCGCACGTCGGTGCcg        <  2:139468/150‑1 (MQ=255)                                                                                                                                                  ccAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTCGCCGTTGGCGACAGCAGTGCCCTGCACTTCTGCCGTCATGCGCTTGAGGCCCATGTGGCGCACGTCGGTGCCGCGCAcc  >  1:283391/1‑150 (MQ=255)                                                                                                                                                    aaGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACAt                                                                                           >  1:653143/1‑59 (MQ=255)                                                                                                                                                    aaGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACAt                                                                                           <  2:653143/59‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   ATTCACTTTACTCATCGATACTCCTTTTGAATCCCGACTAGCCTACTGCCAATGATGGCCACTTGCTACAACCTGGTCACGCCGGGACTTCTTCACATCGTTCGCGGGTGCCTGCCGGCTCTCGACCGCAAACGAAAACGCCCGGCCAAGGCCGGGCGTCGATGCTCGGGAGCAACCTTACTTATCGTCAGATTCACCCGGAACATTAGCCTTTTCGCCGTTGGCGACAGCAGTGCCCTGCACTTCTGCCGTCATGCGCTTGAGGCCCATGTGGCGCACGTCGGTGCCGCGCACC  >  NC_002947/6070686‑6070980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑