Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,080,201 A→T 8.3% Q30L (CAG→CTG)  PP_5332 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,080,2010AT 8.3% 136.2 / 4.7 48Q30L (CAG→CTG) PP_5332hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (25/19);  new base T (2/2);  total (27/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.26e-01

CGGTAAACTCATTGCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAAC‑CGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTGGCAACCTGCAGCGCAACCC  >  NC_002947/6080052‑6080348
                                                                                                                                                       |                                                                                                                                                   
cGGTAAACTCATTGCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAAC‑CGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCca                                                                                                                                                     <  1:624489/150‑1 (MQ=255)
 ggTAAACTCATTGCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACGTTACAGGTCGTAAC‑CGAGCAACATGGCGAACCCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCGcag                                                                                                                                                    >  2:389413/1‑150 (MQ=255)
       cTCATTGCATCTGCCCGGGTCCAAGTCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAAC‑CGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGgc                                                                                                                                              >  2:167171/1‑150 (MQ=255)
               aTCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAAC‑CGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGTGCGCCCTGCTGGGCGATCAgga                                                                                                                                      <  1:440938/150‑2 (MQ=255)
                            cAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAAC‑CGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACcag                                                                                                                         >  1:450404/1‑150 (MQ=255)
                             aaGGCCCCCTGGTCACCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTTGCTCTTCCGATCTGTCG‑‑CTCATAGTTCTGGCGTATCATCAGTGCCCGTACCGCACTCCTTTTCGCTCACCAGGGCGCACAGGTCCTCAGCTACcagc                                                                                                                        <  1:779613/150‑1 (MQ=255)
                                   cACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAAC‑CGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAgg                                                                                                                                                <  1:703040/120‑1 (MQ=255)
                                   cACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAAC‑CGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAgg                                                                                                                                                >  2:703040/1‑120 (MQ=255)
                                   cACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAAC‑CGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAg                                                                                                                  <  2:13246/150‑1 (MQ=255)
                                     cTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAAC‑CGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGc                                                                                                                <  2:80394/150‑1 (MQ=255)
                                          aatcaatGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAAC‑CGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAgg                                                                                                                                       >  2:79663/1‑122 (MQ=255)
                                          aatcaatGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAAC‑CGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAgg                                                                                                                                       <  1:79663/122‑1 (MQ=255)
                                                   gaagaaCGCAATGGCTACCTTTCAGGTCGTAAC‑CGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCgctgct                                                                                                  >  1:157102/1‑150 (MQ=255)
                                                   gaagaaCGCAATGGCTACCTTTCAGGTCGTAAC‑CGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCgctgct                                                                                                  >  2:754391/1‑150 (MQ=255)
                                                   gaagaaCGCAATGGCTACCTTTCAGGTCGTAAC‑CGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCgctgct                                                                                                  <  1:167171/150‑1 (MQ=255)
                                                          gCAATGGCTACCTTTCAGGTCGTAAC‑CGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCATGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTg                                                                                           >  2:85331/1‑150 (MQ=255)
                                                                gCTACCTTTCAGGTCGTAAC‑CGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGAc                                                                                     <  2:620535/150‑1 (MQ=255)
                                                                  tACCTTTCAGGTCGTAAC‑CGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCTGCTGGgcgatc                                                                                                                                          >  2:588044/1‑92 (MQ=255)
                                                                  tACCTTTCAGGTCGTAAC‑CGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCTGCTGGgcgatc                                                                                                                                          <  1:588044/95‑4 (MQ=255)
                                                                  tACCTTTCAGGTCGTAAC‑CGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAAccgc                                                                                                         <  2:723601/128‑1 (MQ=255)
                                                                  tACCTTTCAGGTCGTAAC‑CGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAAccgc                                                                                                         >  1:723601/1‑128 (MQ=255)
                                                                      tttCAGGTCGTAAC‑CGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGcc                                                                               >  1:637743/1‑150 (MQ=255)
                                                                           ggTCGTAAC‑CGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGt                                                                                                                                      >  1:160684/1‑90 (MQ=255)
                                                                           ggTCGTAAC‑CGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGt                                                                                                                                      <  2:160684/90‑1 (MQ=255)
                                                                               gTAAC‑CGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTACTGTGGCTGAGCGACCAGGCCGTCTTCCGc                                                                      >  1:371199/1‑150 (MQ=255)
                                                                                  aC‑CGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGc                                                                      >  1:240451/1‑147 (MQ=255)
                                                                                  aC‑CGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGc                                                                      <  2:240451/147‑1 (MQ=255)
                                                                                   c‑cGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAgg                                                                  >  2:89098/1‑150 (MQ=255)
                                                                                   c‑cGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAgg                                                                  >  1:4212/1‑150 (MQ=255)
                                                                                     cGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAAGTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAggg                                                                 >  2:592527/1‑150 (MQ=255)
                                                                                     cGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAggg                                                                 <  1:485756/150‑1 (MQ=255)
                                                                                           aCATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTc                                                           >  1:522381/1‑150 (MQ=255)
                                                                                                               tgGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCg                                        >  2:39640/1‑150 (MQ=255)
                                                                                                                 gCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACTggg                                      <  1:229462/150‑2 (MQ=255)
                                                                                                                 gCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGgt                                      >  2:159046/1‑150 (MQ=255)
                                                                                                                            gTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCAt                           >  1:445140/1‑150 (MQ=255)
                                                                                                                             tGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATg                          <  2:450404/150‑1 (MQ=255)
                                                                                                                             tGACCGTGCCGAACTACTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATg                          >  1:424938/1‑150 (MQ=255)
                                                                                                                                 cGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGttt                      <  1:389413/150‑1 (MQ=255)
                                                                                                                                  gTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTg                     <  1:39640/150‑1 (MQ=255)
                                                                                                                                  gTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTg                     <  1:156219/150‑1 (MQ=255)
                                                                                                                                           tCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCGGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGAACCGGTGTGCTGGCCATGGTTTGGCAACCTGc            >  2:783362/1‑150 (MQ=255)
                                                                                                                                            ccTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTGGCAACCTGCa           <  2:637743/150‑1 (MQ=255)
                                                                                                                                             ctgtgcGCCCTGCTGGGCGATCAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTGGCAACCTGCAg          >  1:515625/6‑150 (MQ=255)
                                                                                                                                              tGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTGGCAACCTGCAgc         >  1:714743/1‑150 (MQ=255)
                                                                                                                                                aTCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCgg                                               <  1:616656/110‑1 (MQ=255)
                                                                                                                                                aTCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCgg                                               >  2:616656/1‑110 (MQ=255)
                                                                                                                                                   gCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTGGCAACCTGCAGCGCAAc    <  2:157102/150‑1 (MQ=255)
                                                                                                                                                    cccAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTGGCAACCTGCAGCGCAAcc   >  1:549652/1‑150 (MQ=255)
                                                                                                                                                     ccAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTGGCAACCTGCAGCGCAAccc  >  1:412366/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                   
CGGTAAACTCATTGCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAAC‑CGAGCAACATGGCGAACTCA‑ACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTGGCAACCTGCAGCGCAACCC  >  NC_002947/6080052‑6080348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑