| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,081,045 | T→A | 16.8% | intergenic (+36/+26) | PP_5332 → / ← PP_5333 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,081,045 | 0 | T | A | 16.8% | 83.0 / 11.2 | 37 | intergenic (+36/+26) | PP_5332/PP_5333 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (15/15); new base A (3/3); total (19/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01 | |||||||||||
CAGCGAATGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCA‑TTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCGGGGTGTAG > NC_002947/6080902‑6081191 | cAGCGAATGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCcgc < 1:469540/150‑1 (MQ=255) aaTGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCAAGCCCGCGAaga < 1:444662/150‑1 (MQ=255) gAATGTGTGGGATGACCGGGTGGAGTTGAAGCGTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAgg < 2:379795/150‑1 (MQ=255) aaTGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGt > 2:462844/1‑150 (MQ=255) tgtgtgGGATGACCTGGTGGAGTTGAAGCCTGGGGCATAGGGTTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCt < 1:7697/150‑1 (MQ=255) tgtgGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGc > 2:573801/1‑150 (MQ=255) tgtgGGATGACCTGGTGGAGTCGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGc > 2:325139/1‑150 (MQ=255) ggATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTTTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCt < 1:256199/150‑1 (MQ=255) ggATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCt < 1:45224/150‑1 (MQ=255) ggATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCt > 1:721162/1‑150 (MQ=255) cTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGCGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTccacca > 2:389708/1‑150 (MQ=255) tggAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCt > 2:445976/1‑150 (MQ=255) tGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTTCTCTTTTTTGACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCtt < 1:13522/150‑1 (MQ=255) gAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTg > 1:747453/1‑150 (MQ=255) tCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCa < 1:325139/150‑1 (MQ=255) gATCGGTTGTGAAAATATCTGAGCCCTGCTGCATTATTGGTCGCTTCTCCGTGAGATCGGCGGCGCACCCGTCTGCGCAATTATCAAAGCTCTGCGTCTTCCACGACTCTCACCTTGTCTGCACCCAGCGCATAGGCCGCATCGGCCAgg > 1:14477/1‑150 (MQ=255) aTCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCAAGCCCGCGa < 2:260965/65‑1 (MQ=37) aTCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCAAGCCCGCGa > 1:260965/1‑65 (MQ=37) gTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCA‑tt > 2:413652/1‑150 (MQ=255) tgtgAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCA‑TTGc < 2:144706/150‑1 (MQ=255) gtgAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCA‑TTGCt < 1:462844/150‑1 (MQ=255) atatCTGAGCCCCTCTCTTTTTGTACCGGCCTCTTCGTGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCA‑TTGCTGACCtt < 2:747453/150‑1 (MQ=255) atCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCA‑TTGCTGACCTtct < 2:721162/150‑1 (MQ=255) tCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGATGGGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCATCGCATAGGCCGCATCGGCCAGGTCA‑TTGCTGACCTtctt > 2:754250/1‑149 (MQ=255) tCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCA‑TTGCTGACCTtctc > 1:9898/1‑150 (MQ=255) tGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCA‑TTGCTGACCTTCTCCa > 2:731897/1‑150 (MQ=255) cccTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCA‑TTGCTGACCTtctc > 1:683383/1‑144 (MQ=255) cccTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCA‑TTGCTGACCTtctc < 2:683383/144‑1 (MQ=255) tACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCA‑TTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGa < 2:14477/150‑1 (MQ=255) ccGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTGTCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCA‑TTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGAcc > 2:578457/1‑150 (MQ=255) ctctTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCA‑TTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAg > 1:188263/1‑150 (MQ=255) tctTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCA‑TTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGc > 2:96645/1‑150 (MQ=255) ctTCGCGGGCAAGCCCTCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCA‑TTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCg > 2:797059/1‑150 (MQ=255) ttCGCGGGCAAGCCCGCGAAGAGGCCGGTGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGcc > 1:262383/1‑91 (MQ=38) ttCGCGGGCAAGCCCGCGAAGAGGCCGGTGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGcc < 2:262383/91‑1 (MQ=255) cgGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCA‑TTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCGGGgtg < 1:573801/150‑1 (MQ=255) gCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCTCCTTGTCTGCATCCAGCGAATCGGCAGCATCGGCGTATTCACGTCCTG‑CGTTGTCCACCTTCAGCTTGCCGCTGACCCACAGCGGGGTGTAg < 1:413652/150‑1 (MQ=255) | CAGCGAATGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCA‑TTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCGGGGTGTAG > NC_002947/6080902‑6081191 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |