| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,107,734 | G→C | 9.7% | R159G (CGC→GGC) | pdxY ← | pyridoxamine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,107,734 | 0 | G | C | 9.7% | 121.9 / 3.7 | 41 | R159G (CGC→GGC) | pdxY | pyridoxamine kinase |
| Reads supporting (aligned to +/- strand): ref base G (17/20); new base C (2/2); total (19/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
GCTGTGCTCGGCGGTCACCAGCAGCATTTCGAAATGCTCTTCGGCCCGCCCGGGGTACGCCAGGTGCTTGACCAGTACAACCTGTGGTCCACGCTGCAGCAGGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGGATGTCCGCTGTGGCTGCAGCCTCGTCAAGCAGAAACTCACTGACTTCGGGCGGCACGATGCAGCCCTTTTCCGGGTGCCCCATGACCGGGTCGCACAGG > NC_002947/6107591‑6107876 | gCTGTGCTCGGCGGTCACCAGCAGCATTTCGAAATGCTCTTCGGCCCGCCCGGGGTACGCCAGGTGCTTGACCAGTACAACCTGTGGTCCACGCTGCAGCAGGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCGGCGccc > 1:728511/1‑150 (MQ=255) tgCTCGGCGGTCACCAGCAGCATTTCGAAATGCTCTTCGGCCCGCCCGGGGTACGCCAGGTGCTTGACCAGTACAACCTGTGGTCCACGCTGCAGCAGGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCCGCGCCcagtc < 2:791243/150‑4 (MQ=255) cTCGGCGGTCACCAGCAGCATTTCGAAATGCTCTTCGGCCCGCCCGGGGTACGCCAGGTGCTTGACCAGTACAACCTGTGGTCCACGCTGCAGCAGGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGaa > 1:241946/1‑150 (MQ=255) cTCGGCGGTCACCAGCAGCATTTCGAAATGCTCTTCGGCCCGCCCGGGGTACGCCAGGTGCTTGACCAGTACAACCTGTGGTCCACGCTGCAGCAGGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGaa < 2:110487/150‑1 (MQ=255) ggTCACCAGCAGCATTTCGAAATGCTCTTCGGCCCGCCCGGGGTACGCCAGGTGCTTGACCAGTACAACCTGTGGTCCACGCTGCAGCAGGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGAAGCTGTc < 1:129355/150‑1 (MQ=255) gcagcaTTTCGAAATGCTCTTCGGCCCGCCCGGGGTACGCCAGGTGCTTGACCAGTACAACCTGTGGTCCACGCTGCAGCAGGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCa > 2:475712/1‑150 (MQ=255) caTTTCGAAATGCTCTTCGGCCCGCCCGGGGTACGCCAGGTGCTTGACCAGTACAACCTGTGGTCCACGCTGCAGCAGGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGaa < 1:73617/132‑1 (MQ=255) caTTTCGAAATGCTCTTCGGCCCGCCCGGGGTACGCCAGGTGCTTGACCAGTACAACCTGTGGTCCACGCTGCAGCAGGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGaa > 2:73617/1‑132 (MQ=255) tttCGAAATGCTCTTCGGCCCGCCCGGGGTACGCCAGGTGCTTGACCAGTACAACCTGTGGTCCACGCTGCAGCAGGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGt > 2:126705/1‑150 (MQ=255) gCTCTTCGGCCCGCCCGGGGTACGCCAGGTGCTTGACCAGTACAACCTGTGGTCCACGCTGCAGCAGGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGcaca < 1:568816/150‑1 (MQ=255) gcccgcccGGGGTACGCCAGGTGCTTGACCAGTACAACCTGTGGTCCACGCTGCAGCAGGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGGATGTcc > 2:250213/1‑150 (MQ=255) cccgGGGTACGCCAGGTGCTTGACCAGTACAACCTGTGGTCCACGCTGCAGCAGGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGGATGTCCGCtgt < 1:173135/150‑1 (MQ=255) gTACGCCAGGTGCTTGACCAGTACAACCTGTGGTCCACGCTGCAGCAGGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAg < 1:441755/111‑1 (MQ=255) gTACGCCAGGTGCTTGACCAGTACAACCTGTGGTCCACGCTGCAGCAGGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAg > 2:441755/1‑111 (MQ=255) tACGCCAGGTGCTTGACCAGTACAACCTGTGGTCCACGCTGCAGCAGGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGGATGTCCGCTGTGGCTGCa < 2:594540/150‑1 (MQ=255) cGCCAGGTGCTTGACCAGTACAACCTGTGGTCCACGCTGCAGCAGGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGGATGTCCGCTGTGGCTGCAGc < 1:475712/150‑1 (MQ=255) aGGTGCTTGACCAGTACAACCTGTGGTCCACGCTGCAGCAGGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGGATGTCCGCTGTGGCTGCAGCCTCg > 2:159522/1‑150 (MQ=255) tGCTTGACCAGTACAACCTGTGGTCCACGCTGCAGCAGGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGGATGTCCGCTGTGGCTGCAGCCTCGTCa < 2:629762/150‑1 (MQ=255) gCTTGACCAGTACAACCTGTGGTCCACGCTGCAGCAGGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGGATGTCCGCTGTGGCTGCAGCCTCGTCaa < 1:159522/150‑1 (MQ=255) gCTTGACCAGTACAACCTGTGGTCCACGCTGCAGCAGGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGGATGTCCGCTGTGGCTGCAGCCTCGTCaa < 2:164893/150‑1 (MQ=255) tGACCAGTACAACCTGTGGTCCACGCTGCAGCAGGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGGATGTCCGCTGTGGCTGCAGCGTCGTCAAGCa < 1:589839/150‑1 (MQ=255) tGACCAGTACAACCTGTGGTCCACGCTGCAGCAGGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGGATGTCCGCTGTGGCTGCAGCCTCGTCAAGCa > 1:61921/1‑150 (MQ=255) cTGTGGTCCACGCTGCAGCAGGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGGATGTCCGCTGTGGCTGCAGCCTCGTCAAGCAGAAACTCACTGAc > 2:17427/1‑150 (MQ=255) tgtgGTCCACGCTGCAGCAGGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGGATGTCCGCTGTGGCTGCAGCCTCGTCAAGCAGAAACTCACTGACt > 1:648782/1‑150 (MQ=255) tCCACGCTGCAGCAGGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGGATGTCCGCTGTGGCTGCAGCCTCGTCAAGCAGAAACTCACt > 2:431165/1‑141 (MQ=255) tCCACGCTGCAGCAGGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGGATGTCCGCTGTGGCTGCAGCCTCGTCAAGCAGAAACTCACt < 1:431165/141‑1 (MQ=255) tCCACGCTGCAGCAGGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGGATGTCCGCTGTGGCTGCAGCCTCGTCAAGCAGAAACTCACTGACTTCggg > 1:724383/1‑150 (MQ=255) cagcagGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGGATGTCCGCTGTGGCTGCAGCCTCGTCAAGCAGAAACTCACTGACTTCGGGCGGCACGAt < 2:648782/150‑1 (MQ=255) gTTCACGCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGGATGTCCGCTGTGGCTGCAGCCTCGTCAAGCAGAAACTCACTGACTTCGGGCGGCACGATGCAGCCCTTTTCCGGGTg > 1:64756/1‑150 (MQ=255) gCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGGATGt < 1:387088/71‑1 (MQ=255) gCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGGATGt > 2:387088/1‑71 (MQ=255) tcctccAGCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGGATGTCCGCTGTGGCTGCAGCCTCGTCAAGCAGAAACTCACTGACTTCGGGCGGCACGATGCAGCCCTTTTCCGGGTGCCCCATGAcc < 2:64756/150‑1 (MQ=255) ctccAGCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGGATGTCCGCTGTGGCTGCAGCCTCGTCAAGCAGAAACTCACTGACTTCGGGCGGCACGATGCAGCCCTTTTCCGGGTGCCCCATGACCgg < 1:250213/150‑1 (MQ=255) ttctgtGGGCGCCGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGGATGTCCGCTGTGGCTGCAGCCTCGTCAAGCAGAAACTCACTGACTTCGGGCGGCACGATGCAGCCCTTTTCCGGGTGCCCCATGACCGGGt < 1:574483/142‑1 (MQ=255) ttctgtGGGCGCCGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGGATGTCCGCTGTGGCTGCAGCCTCGTCAAGCAGAAACTCACTGACTTCGGGCGGCACGATGCAGCCCTTTTCCGGGTGCCCCATGACCGGGt > 2:574483/6‑147 (MQ=255) ttctgtGGGCGCCGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGGATGTCCGCTGTGGCTGCAGCCTCGTCAAGCAGAAACTCACTGACTTCGGGCGGCACGATGCAGCCCTTTTCCGGGTGCCCCATGACCGGGTCGc > 1:385878/6‑150 (MQ=255) gCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGGATGTcc > 2:537291/1‑62 (MQ=255) gCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGGATGTcc < 1:537291/62‑1 (MQ=255) aCTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGGATGTCCGCTGTGGCTGCAGCCTCGTCAAGCAGAAACTCACTGACTTCGGGCGGCACGATGCAGCCCTTTTCCGGGGGCCCCATGACCGGGTCGcaca < 1:17427/150‑1 (MQ=255) aCTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCAAGCTCGTTGAGCTCCCGGACGCCCGCTGTTACTGCAGCCACGGCAAGCAGAAACTCACTGGCTTCGGGCGGCACGATGCAGCCCTTTTCCGGGTGCCCCATGACCGGGTCGcaca > 1:613489/1‑150 (MQ=255) tGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGAATGTCCGCTGTGGCTGCAGCCTCGTCAAGCAGAAACTCACTGACTTCGGGCGGCACGATGCAGCCCTTTTCCGGGTGCCCCATGACCGGGTCGCACAgg < 2:728511/150‑1 (MQ=255) | GCTGTGCTCGGCGGTCACCAGCAGCATTTCGAAATGCTCTTCGGCCCGCCCGGGGTACGCCAGGTGCTTGACCAGTACAACCTGTGGTCCACGCTGCAGCAGGCTGCGCGCCATGTTCACGCAATCCTCCAGCGACTGGGCGCGGCGCCCACAGAAGCTGTCCAGTTCCAGCTGGTTGGGGCACAGGATGTCCGCTGTGGCTGCAGCCTCGTCAAGCAGAAACTCACTGACTTCGGGCGGCACGATGCAGCCCTTTTCCGGGTGCCCCATGACCGGGTCGCACAGG > NC_002947/6107591‑6107876 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |