| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 431,358 | A→G | 18.2% | L320P (CTG→CCG) | PP_0354 ← | CBS domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 431,358 | 0 | A | G | 18.2% | 65.6 / 9.4 | 33 | L320P (CTG→CCG) | PP_0354 | CBS domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (12/15); new base G (3/3); total (15/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.16e-01 | |||||||||||
CGGGTCGCCACGTTCGGCCAGGGCCAGTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGTTTTCCACCAGGCAGACATGGGCAATA > NC_002947/431216‑431501 | cGGGTCGCCACGTTCGGCCAGGGCCAGTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAAc < 1:531123/150‑1 (MQ=255) tCGCCACGTTCGGCCAGGGCCAGTTCGCTGGCCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGTGTACGTTTGTCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGc < 1:336994/150‑1 (MQ=255) ccACGTTCGGCCAGGGCCAGTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTa > 1:225404/1‑150 (MQ=255) gccagggccagTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGt > 1:6397/1‑150 (MQ=255) ccagggccagTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTg < 1:540299/150‑1 (MQ=255) cagTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATg < 2:6397/150‑1 (MQ=255) aTGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATg < 2:707681/150‑1 (MQ=255) cccGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTgc > 2:173761/1‑150 (MQ=255) cacaGTATGGTCGTTGAGCAAGGTACTTGTGTTGGTGATCTGCCTCGACGATGCGCCATGGGCGTGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAg < 1:201894/150‑1 (MQ=255) ggTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTg > 1:520910/1‑150 (MQ=255) cGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCTCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGAt < 2:717855/150‑1 (MQ=255) cGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGAt < 1:86521/150‑1 (MQ=255) gTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGGTTTCGCTGCGCGGCGAAATCAGc > 1:410482/1‑90 (MQ=37) gTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGGTTTCGCTGCGCGGCGAAATCAGc < 2:410482/90‑1 (MQ=38) aaTGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGTGCCAGGTGAACCAGATCGACCCGTTGCAg < 2:554674/150‑1 (MQ=255) gATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAgggg > 2:520933/1‑149 (MQ=255) cTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGaa < 2:413278/150‑1 (MQ=255) tGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGga > 1:221847/1‑142 (MQ=255) tGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGga < 2:221847/142‑1 (MQ=255) gTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCg < 1:173761/150‑1 (MQ=255) gaTGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAAcc > 1:483186/1‑150 (MQ=255) gcCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATgg > 2:43275/1‑79 (MQ=255) gcCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATgg < 1:43275/79‑1 (MQ=255) gcCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGACGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCAcgcc > 1:759790/1‑150 (MQ=255) cATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCAcgccgc > 2:486427/1‑150 (MQ=255) gCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTc > 1:753559/1‑150 (MQ=255) ttCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCCCGGCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGTTTTc < 1:695748/150‑1 (MQ=255) tggtttCGCTGCGCGGCGAAATCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGCTCGACCCGTTGCAGGgag > 1:651607/4‑92 (MQ=38) tggtttCGCTGCGCGGCGAAATCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGgag < 2:651607/89‑1 (MQ=38) tggtttCGCTGCGCGGCGAAATCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAg < 2:163650/121‑1 (MQ=255) tggtttCGCTGCGCGGCGAAATCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAg > 1:163650/4‑124 (MQ=255) gccgcGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGTTTTCCACCAGGCAGACATGGGCAat < 2:520910/150‑1 (MQ=255) ccgcGCAGCGAAACCAGCGTATCCACGCGTGGCGCATGGCGTATGGTCCGCGCCAGTTGAACCAGATCGACCCGTTGCAAGGAAAACAGGTCGCGCTCGGAAACCACGCCGCGCCGGCCTCCGTGTTGCCCCAGGCGCACATGGCCGata > 2:124528/1‑150 (MQ=255) | CGGGTCGCCACGTTCGGCCAGGGCCAGTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGTTTTCCACCAGGCAGACATGGGCAATA > NC_002947/431216‑431501 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |