Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 464,325 G→C 9.8% H259D (CAC→GAC) ‡ davB ← lysine 2‑monooxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947464,3250GC 9.8% 120.8 / 5.6 41H259D (CAC→GAC) ‡davBlysine 2‑monooxygenase
Reads supporting (aligned to +/- strand):  ref base G (21/16);  new base C (2/2);  total (23/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.90e-01

GCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGAGCGCGAGGTGG  >  NC_002947/464180‑464474
                                                                                                                                                 |                                                                                                                                                     
gcgcGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCtgg                                                                                                                                                   <  2:355522/150‑1 (MQ=255)
                  aCCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCAATCCGGTAAATCGCGCCAGATTTGTGGGGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGtc                                                                                                                                 <  1:297271/150‑1 (MQ=255)
                            gcgGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCAcgc                                                                                                                       <  1:452937/150‑1 (MQ=255)
                                tGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGCGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGaa                                                                                                                   <  1:620738/150‑1 (MQ=255)
                                      gccATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTc                                                                                                             <  1:623004/150‑1 (MQ=255)
                                         aTGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGCACCTGGTGgtcggg                                                                                                                                           <  2:337989/117‑3 (MQ=255)
                                         aTGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGCACCTGGTGgtcggg                                                                                                                                           >  1:337989/1‑115 (MQ=255)
                                           gCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCtggtg                                                                                                                                                 <  2:781595/109‑1 (MQ=255)
                                           gCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCtggtg                                                                                                                                                 >  1:781595/1‑109 (MQ=255)
                                              gCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCg                                                                                                     >  2:660455/1‑150 (MQ=255)
                                                  gCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGATGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGtt                                                                                                 <  2:571115/150‑1 (MQ=255)
                                                    tCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTTCAGCATCGAGTTgg                                                                                               >  1:598334/1‑150 (MQ=255)
                                                            gTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTCGGGGAAGTcc                                                                                       >  2:596515/1‑150 (MQ=255)
                                                             tGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCGTCGAGTTGGGGAAGTCCg                                                                                      >  1:664369/1‑150 (MQ=255)
                                                               cccTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTg                                                                                                                                    <  2:771441/102‑1 (MQ=255)
                                                               cccTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTg                                                                                                                                    >  1:771441/1‑102 (MQ=255)
                                                                 ctctGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTc                                                                                  <  1:660455/150‑1 (MQ=255)
                                                                      gCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTggtggt                                                                                                                                               >  2:137429/1‑84 (MQ=255)
                                                                      gCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTggtggt                                                                                                                                               <  1:137429/84‑1 (MQ=255)
                                                                              aCGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCaa                                                                              <  1:472423/141‑1 (MQ=255)
                                                                              aCGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCaa                                                                              >  2:472423/1‑141 (MQ=255)
                                                                                gCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGcc                                                                   <  1:518748/150‑1 (MQ=255)
                                                                                  aGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCACGACCACCAGCACCTGGTGgtcgggg                                                                                                                                          >  1:75452/1‑74 (MQ=25)
                                                                                  aGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCACGACCACCAGCACCTGGTGgtcgggg                                                                                                                                          <  2:75452/77‑4 (MQ=25)
                                                                                                       aGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACtt                                            >  1:394691/1‑150 (MQ=255)
                                                                                                             cTTGTGGCACTTGTTCCACGCGCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGt                                      >  2:78000/1‑150 (MQ=255)
                                                                                                             cTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGt                                      >  1:68140/1‑150 (MQ=255)
                                                                                                             cTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAATTGGGGAAGTCCAAGTGCCAACCGCCGGTTCCGAAACCGACCTGGCCGACCACTTCGCAGt                                      >  2:122283/1‑150 (MQ=255)
                                                                                                              ttGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGAGTTCGAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGACACCGACCTGGCCGAAAACTTCGCgggg                                     >  2:549996/1‑148 (MQ=255)
                                                                                                                     aCTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAg                              >  2:327927/1‑150 (MQ=255)
                                                                                                                      cTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGc                             >  1:257408/1‑150 (MQ=255)
                                                                                                                         gTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGtc                                                                                                                                 <  2:298577/47‑1 (MQ=255)
                                                                                                                         gTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGtc                                                                                                                                 >  1:298577/1‑47 (MQ=255)
                                                                                                                            ccACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCt                       >  1:71776/1‑150 (MQ=255)
                                                                                                                             gaCGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGCAGATTTCAAGCGTCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGACCACTTCGCGGTGCTGGAAGCTCAGCtt                      >  2:325731/2‑150 (MQ=255)
                                                                                                                             cACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTggg                                                                                              <  1:244745/78‑1 (MQ=255)
                                                                                                                             cACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTggg                                                                                              >  2:244745/1‑78 (MQ=255)
                                                                                                                              aCGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTg                     <  1:327927/150‑1 (MQ=255)
                                                                                                                                 cccccGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAGTTCCGAGTCCCAACCGACGGTCACAATAACCTCAACGCTGTGGACTTTGCGGTGCTGGAAGCTCAGCTTGGCa                  >  1:775748/1‑150 (MQ=255)
                                                                                                                                           ccaGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTcc                                                                                       >  2:250455/1‑71 (MQ=255)
                                                                                                                                           ccaGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTcc                                                                                       <  1:250455/71‑1 (MQ=255)
                                                                                                                                                 gCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGAGCGCGAGGTgg  <  1:78000/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
GCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGAGCGCGAGGTGG  >  NC_002947/464180‑464474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑