| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 471,069 | T→C | 23.3% | intergenic (‑110/+18) | PP_0386 ← / ← rpoD | sensory box protein/RNA polymerase sigma 70 factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 471,069 | 0 | T | C | 23.3% | 51.0 / 11.4 | 30 | intergenic (‑110/+18) | PP_0386/rpoD | sensory box protein/RNA polymerase sigma 70 factor |
| Reads supporting (aligned to +/- strand): ref base T (8/15); new base C (4/3); total (12/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
GGATCAGCAACAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTG > NC_002947/470926‑471218 | ggATCAGCAACAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCg > 2:587026/1‑150 (MQ=255) aTCAGCAACAGAATGGCCGGCTTCAAGGACATGGCATCCTCGCACTCAGGTGGTCCACATGCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCCAGGCCggg < 1:8004/150‑1 (MQ=255) aTCAGCAACAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCggg < 2:575012/150‑1 (MQ=255) tCAGCAACAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCCAGGCCgggg < 1:146969/150‑1 (MQ=255) aaCAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTg > 1:284810/1‑150 (MQ=255) aCAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCCAGGCCgggg > 1:175854/1‑144 (MQ=255) aCAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCCAGGCCgggg < 2:175854/144‑1 (MQ=255) ggCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACtcgtc < 2:452003/150‑1 (MQ=255) cTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGa < 2:284810/150‑1 (MQ=255) cTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGa < 2:270911/150‑1 (MQ=255) ggACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCggg < 1:507847/124‑1 (MQ=255) ggACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCggg > 2:507847/1‑124 (MQ=255) tGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTAGAGGAAGGAgcgc > 2:743159/1‑150 (MQ=255) cTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCt < 2:538706/150‑1 (MQ=255) tCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGag < 1:472145/126‑1 (MQ=255) tCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGag > 2:472145/1‑126 (MQ=255) aGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCtt < 1:748018/150‑1 (MQ=255) gtgtAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTg < 1:511872/150‑1 (MQ=255) gtgCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGa < 1:104051/150‑1 (MQ=255) aaGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCg < 2:276853/93‑1 (MQ=255) aaGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCg > 1:276853/1‑93 (MQ=255) gCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCa < 1:743159/150‑1 (MQ=255) tATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGGTGCTCTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTAc < 1:7824/150‑1 (MQ=255) tgacaaaaCCCCGGCCCAGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCa > 1:666777/5‑150 (MQ=255) aaaaCCCCGGCCCAGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGGTCCGCTGGCGCGTTACGGCAAACTGCTTGCCCACctc > 2:130549/1‑150 (MQ=255) ccccGGCCCAGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCg > 2:392177/1‑150 (MQ=255) cccGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGa > 1:514833/1‑150 (MQ=255) cGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCcttc < 1:666678/90‑1 (MQ=255) cGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCcttc > 2:666678/1‑90 (MQ=255) cGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAgg < 2:775752/150‑1 (MQ=255) tGGGCCGGGGTTTTGTCATCCCTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGtgtg > 2:106788/1‑150 (MQ=255) | GGATCAGCAACAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTG > NC_002947/470926‑471218 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |