| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 524,355 | G→C | 21.2% | intergenic (+219/‑597) | tyrS → / → PP_16SC | tyrosine‑‑tRNA ligase/16S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 524,355 | 0 | G | C | 21.2% | 65.8 / 12.4 | 33 | intergenic (+219/‑597) | tyrS/PP_16SC | tyrosine‑‑tRNA ligase/16S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base G (14/12); new base C (2/5); total (16/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01 | |||||||||||
GTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGC‑‑CTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCA > NC_002947/524207‑524497 | gtggtTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCgcggg < 2:114953/150‑1 (MQ=255) tgttgtAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTc > 1:161674/1‑150 (MQ=255) gtAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCCCGCGCGGCGCTCGGtt < 2:764549/150‑2 (MQ=255) gggagggggCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCCCGCGCGGCGCTCGGTTTCATa < 2:95589/150‑1 (MQ=255) gggCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGTCATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCt > 1:529659/1‑150 (MQ=255) gCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGa > 1:294884/1‑150 (MQ=255) tGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAGACCTAGCGCCGCCCGCGCGTCGCTCGGTTTCATAGGCGGTGaat < 1:217743/150‑2 (MQ=21) ttttGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACt < 2:593797/150‑1 (MQ=255) ttGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCAGCGCCTATGAGACCGAGCGCCGCCCGCGCGGCGCTCGGTTTCATAGGCGGTGaatgtct < 1:256792/150‑6 (MQ=25) tGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTc < 2:294884/150‑1 (MQ=255) gggTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGc < 2:131685/150‑1 (MQ=255) tCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGCAACTGTAGTGGCGAACACTAGGTAGC‑‑CTCGAtt < 2:446943/150‑2 (MQ=255) tCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGC‑‑CTCGATa > 2:298554/1‑150 (MQ=255) gCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGC‑‑CTCGATACgg > 2:99529/1‑150 (MQ=255) gCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCCCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGC‑‑CTCGATACgg < 1:99529/150‑1 (MQ=255) aaCCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGC‑‑CTCGATACGGTCttt > 1:64158/1‑150 (MQ=255) ccTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGC‑‑CTCGATACGGTCTTTTcc < 2:161674/150‑1 (MQ=255) cAGCGCCTGTGAGATCGAGCGCCGCCCGCGTGCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTGTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGC‑‑CTCGATACGGTCTTTTCCCCTta > 1:352748/1‑150 (MQ=255) cGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGC‑‑CTCGATACGGTCTTTTCCCCTTATATTTCACTTTCttt > 2:564526/1‑149 (MQ=255) gcccgcGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATCGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGC‑‑CTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTaaa < 2:64158/150‑1 (MQ=255) gcccgcGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGaaa < 2:168133/80‑1 (MQ=255) gcccgcGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGaaa > 1:168133/1‑80 (MQ=255) ccgcGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGC‑‑CTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAgg > 1:25438/1‑150 (MQ=255) cGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGTCGCTCGGTCTCATAGGCGCTGAACCTTTCTTGGCGAACACTTGGCAGC‑‑CTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGc < 1:254402/150‑1 (MQ=255) aTTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGC‑‑CTCGATACGGTATCTTCACCTTATATTTCACTTTCTTGAAATTAAAGGTGGACGCCCGCTCGAATc < 2:529659/150‑1 (MQ=17) tGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCAGCATGATAC‑‑ACGTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATTCCCTTATAATgc > 2:27037/1‑150 (MQ=14) aCCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCACTGCAACTGTCGTGGCGAACACTTGGCAGC‑‑CCCGATACGGTCTTTTCCCCTTATATTTCACt < 1:714221/100‑1 (MQ=11) aCCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCACTGCAACTGTCGTGGCGAACACTTGGCAGC‑‑CCCGATACGGTCTTTTCCCCTTATATTTCACt > 2:714221/1‑100 (MQ=11) cGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGc > 2:270600/1‑52 (MQ=255) cGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGc < 1:270600/52‑1 (MQ=255) cGAGCGCCGCCCGCGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGC‑‑CTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATccc < 1:475365/134‑1 (MQ=21) cGAGCGCCGCCCGCGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGC‑‑CTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATccc > 2:475365/1‑134 (MQ=255) cGAGCGCCGCCCGCGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGC‑‑CTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCAGTATAATGCTCCCCa > 1:360239/1‑150 (MQ=11) ccgcGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGC‑‑CTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCa > 1:544441/1‑150 (MQ=255) | GTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGC‑‑CTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCA > NC_002947/524207‑524497 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |