| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 532,980 | A→G | 17.4% | D81G (GAC→GGC) | tufA → | elongation factor Tu‑A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 532,980 | 0 | A | G | 17.4% | 44.7 / 4.2 | 23 | D81G (GAC→GGC) | tufA | elongation factor Tu‑A |
| Reads supporting (aligned to +/- strand): ref base A (11/8); new base G (2/2); total (13/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01 | |||||||||||
TCGCGTTTGCTCCGAAGTTTTCGGTTCGGCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCACAAACCCGTGAGCACATCCTGTTGTCCCGTCAGGTAGGCGTTCCGTACA > NC_002947/532837‑533129 | tCGCGTTTGCTCCGAAGTTTTCGGTTCGGCAATCGTTGAGTTCGACGAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGccc > 2:699741/1‑150 (MQ=255) ttcggttcggCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTg < 2:664890/150‑1 (MQ=35) gCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATg > 1:173227/1‑150 (MQ=35) ggAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCt > 1:172694/1‑150 (MQ=32) aagaaAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTgg < 2:173227/150‑1 (MQ=32) gcgcgcGGTAGCACCAAAAACACCGCTCACGTCGAGTACAACTCGCCCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGCTCCTGGTTTGCTCg < 2:182131/150‑1 (MQ=11) tATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGGCTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGa > 1:505770/1‑150 (MQ=17) aCCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGt > 1:590474/1‑150 (MQ=32) cATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTcc > 1:229910/1‑150 (MQ=32) cGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCcaca > 2:215026/1‑150 (MQ=35) aCGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGGCTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTg > 2:10490/1‑113 (MQ=18) aCGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGGCTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTg < 1:10490/113‑1 (MQ=18) aGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCACAAACCCGTGAGc > 2:614921/1‑150 (MQ=34) tACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACg > 1:464227/1‑94 (MQ=34) tACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACg < 2:464227/94‑1 (MQ=34) cATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCACAAACCCGTGAGCACATCCTGTTGTc > 2:656376/1‑150 (MQ=35) ttCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCACAAACCCGTGAGCACATCCTGTTGTccc < 2:590474/150‑1 (MQ=35) cACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCACAAACCCGTGAGCACATCCTGTTGTCCCGTCAg < 1:433111/150‑1 (MQ=35) aCTACGCTCACGTTGGCTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCACAAACCCGTGAGCACATCCTGTTGTCCCGTCAgg < 2:505770/150‑1 (MQ=25) tACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCACAAACCCGTGAGCACATCCTGTTGTCCCGTCAGGTa > 1:377950/1‑150 (MQ=35) gCTCACGTTGACTGCCCAGCTCACGCTGACTACGTGAAGACCATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCACAAACCCGTGAGCACATCCTGTTGTCCCGTCAGGTAGGc < 1:550243/150‑1 (MQ=25) ttGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCACAAAccc < 1:756137/111‑1 (MQ=33) ttGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCACAAAccc > 2:756137/1‑111 (MQ=33) aCTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCACAAACCCGTGAGCACATCCTGTTGTCCCGTCAGGTAGGCGTTCCGTACa > 2:49696/1‑150 (MQ=35) | TCGCGTTTGCTCCGAAGTTTTCGGTTCGGCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCACAAACCCGTGAGCACATCCTGTTGTCCCGTCAGGTAGGCGTTCCGTACA > NC_002947/532837‑533129 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |