Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947699,7250AG68.3% 35.5 / 28.7 41intergenic (+115/‑129)PP_t06/PP_23SDtRNA‑Ala/23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (10/3);  new base G (18/10);  total (28/13)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.46e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCG  >  NC_002947/699581‑699808
                                                                                                                                                 |                                                                                   
tCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGaa                                                                                >  1:443452/1‑150 (MQ=38)
tCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGGTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGaa                                                                                >  2:713656/1‑150 (MQ=255)
     ggTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCa                                                                           <  2:443452/150‑1 (MQ=255)
     ggTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCa                                                                           <  2:682290/150‑1 (MQ=255)
       ttCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGt                                                                         >  1:645273/1‑150 (MQ=38)
       ttCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGt                                                                         >  1:262441/1‑150 (MQ=38)
        tCGATCCCGCGTGGCTACAGCACTTTCGCCGTACGCAGTTACTTGTCAGTTCTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAaccatgtaa                                                                        <  2:101796/150‑8 (MQ=11)
          gATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATAGGTGATAGATATAGACTCAACACCAGTTTc                                                                      >  1:646531/1‑150 (MQ=255)
           aTCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAac                                                                               >  2:584793/1‑140 (MQ=255)
           aTCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAac                                                                               <  1:584793/140‑1 (MQ=255)
              ccGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCCGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCActg                                                                  >  1:475698/1‑150 (MQ=255)
                      cTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGCACCCCCAATTCCCTGCtggagcg                                                          >  2:876861/1‑146 (MQ=14)
                      cTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGa                                                          >  1:75791/1‑150 (MQ=39)
                       tCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGAt                                                         >  1:788915/1‑150 (MQ=38)
                          accacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAg                                                      <  2:14659/150‑1 (MQ=255)
                          accacTTTCGCCGTACGCAGTTACTTGTCAGAACATAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAg                                                      >  1:897172/1‑150 (MQ=255)
                           ccacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAgg                                                     >  1:478079/1‑150 (MQ=255)
                           ccacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAgg                                                     >  1:400191/1‑150 (MQ=38)
                            cacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCa                                                                           >  1:722209/1‑127 (MQ=40)
                            cacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCa                                                                           <  2:722209/127‑1 (MQ=40)
                               tttCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCa                                                       >  1:764100/1‑144 (MQ=255)
                               tttCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCa                                                       <  2:764100/144‑1 (MQ=255)
                                   gCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTa                                             >  1:868526/1‑150 (MQ=255)
                                             gTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGTTGGATCAGGCTAAGGTAAAATTTGTGa                                   <  1:257717/150‑1 (MQ=38)
                                                 cTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCGGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTc                               >  2:659324/1‑150 (MQ=25)
                                                   tGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTg                             >  2:648181/1‑150 (MQ=35)
                                                    gTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGc                            >  2:602972/1‑150 (MQ=25)
                                                     tCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCt                           >  1:863901/1‑150 (MQ=25)
                                                     tCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATAAGTAAGGGGTCAAGGTCCTAAACCTGTTGCACTGCTGGTGGATCAGGCTAAGGTAAAATTAGTGAGTTCTGCt                           >  1:791356/1‑150 (MQ=11)
                                                      cAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATTAGGATAAGGACAGATTTGTAAGCGCTGCTc                          >  2:877898/1‑150 (MQ=11)
                                                       aGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCg                         >  2:356035/1‑150 (MQ=35)
                                                        gAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGa                        >  1:356260/1‑150 (MQ=25)
                                                          aCTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGaaa                      <  1:502573/150‑1 (MQ=34)
                                                              aGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGc                  <  2:645273/150‑1 (MQ=21)
                                                                aaaTGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCaa                <  2:356260/150‑1 (MQ=21)
                                                                  aTGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAACTTCGGATATGTGATAGATATAGGCTGAGCACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTATGCTCGAAAGAGCAACg              >  1:557940/1‑150 (MQ=255)
                                                                    caaCATTCGAGCT‑GAATGTTGAGTTCTGGCTTTTGTCAGGTCGTTCTTTAGAAATTCGGGTATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTGTGCTCGAAAGAGCAACgga            >  2:718824/2‑148 (MQ=11)
                                                                        aTTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCActgc                                                                 <  2:651697/93‑1 (MQ=255)
                                                                        aTTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCActgc                                                                 >  1:651697/1‑93 (MQ=255)
                                                                               cTCGAATGTTGATTTCTGGCTTTTGTCATATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCg  <  1:192220/150‑1 (MQ=11)
                                                                               cTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAAACCAAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCg  >  1:111213/1‑150 (MQ=2)
                                                                                                                                                 |                                                                                   
TCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCG  >  NC_002947/699581‑699808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑