Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,164,645	T→G	T256P (ACC→CCC)	hexR ←	DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,164,645	0	T	G	100.0%	119.7 / NA	37	T256P (ACC→CCC)	hexR	DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (20/17);  total (20/17)

ACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCTCAGTTGAGGTCGTCGTCCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGTCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAATGTGCAGGCTCAGGCTGCAGGCATTGGCCAGTGGCGAGCCTGCGGCGGTGA  >  NC_002947/1164506‑1164785                                                                                                                                            |                                                                                                                                             aCCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCTCAGTTGAGGTCGTCGTCCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTg                                                                                                                                    <  2:780968/150‑1 (MQ=255)          gCGTGCGCACCTGCGTTTGCAGGCGCACCGGCTCAGTTGAGGTCGTCGTCCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACa                                                                                                                           >  2:749493/1‑150 (MQ=255)            gTGCGCACCTGCGTTTGCAGGCGCACCGGCTCAGTTGAGGTCGTCGTCCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATc                                                                                                                         <  1:574320/150‑1 (MQ=255)              gcgcACCTGCGTTTGCAGGCGCACCGGCTCAGTTGAGGTCGTCGTCCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTACGCAGGTGAGGCGGGAAGTCCCCGCCTCGGCGCAGGGGCATGCCGGTGGCCAGTACAGCgg                                                                                                                       >  2:184767/1‑149 (MQ=255)                gcACCTGCGTTTGCAGGCGCACCGGCTCAGTTGAGGTCGTCGTCCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGc                                                                                                                     >  2:278390/1‑150 (MQ=255)                    cTGCGTTTGCAGGCGCACCGGCTCAGTTGAGGTGGTCGTCCTCGATCGGGTAACGGCTGGCTTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACAGCGCGGCGCAGGGGCATGCCGGTCGCCAGTAAAACGAGCACgg                                                                                                                 >  1:196797/1‑150 (MQ=255)                    cTGCGTTTGCAGGCGCACCGGCTCAGTTGAGGTCGTCGTCCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACgg                                                                                                                 >  1:382958/1‑150 (MQ=255)                             cAGGCGCACCGGCTCAGTTGAGGTCGTCGTCCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGga                                                                                                        <  2:228852/150‑1 (MQ=255)                             cAGGCGCACCGGCTCAGTTGAGGTCGTCGTCCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGga                                                                                                        <  1:749493/150‑1 (MQ=255)                              aGGCGCACCGGCTCAGTTGAGGTCGTCGTCCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGgat                                                                                                       >  2:869828/1‑150 (MQ=255)                              aGGCGCACCGGCTCAGTTGAGGTCGTCGTCCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGgat                                                                                                       >  2:617962/1‑150 (MQ=255)                                       ggCTCAGTTGAGGTCGTCGTCCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGa                                                                                              <  1:204495/150‑1 (MQ=255)                                              ttGAGGTCGTCGTCCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCAtt                                                                                       <  1:617962/150‑1 (MQ=255)                                               tGAGGTCGTCGTCCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGTCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTg                                                                                      <  1:504617/150‑1 (MQ=255)                                                   gtcgtcgtcCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCATGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCAt                                                                                  <  2:438293/150‑1 (MQ=255)                                                      gtcgtcCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTa                                                                               <  2:549509/150‑1 (MQ=255)                                                               gATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGGAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGt                                                                      >  2:341982/1‑150 (MQ=255)                                                               gATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGt                                                                      >  1:377748/1‑150 (MQ=255)                                                                     gTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTc                                                                >  2:747099/1‑150 (MQ=255)                                                                            cTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGc                                                         >  1:565449/1‑150 (MQ=255)                                                                             tGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCg                                                        >  2:898270/1‑150 (MQ=255)                                                                                     aGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAATGTGc                                                >  1:605326/1‑150 (MQ=255)                                                                                      ggCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAg                                                                              <  1:13954/119‑1 (MQ=255)                                                                                      ggCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAg                                                                              >  2:13954/1‑119 (MQ=255)                                                                                         tttCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAATGTGcaggc                                            >  2:295754/1‑150 (MQ=255)                                                                                         tttCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAATGTGcaggc                                            >  2:81031/1‑150 (MQ=255)                                                                                            ccTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAATGTGcaggctca                                         <  1:898270/150‑1 (MQ=255)                                                                                             cTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAATGTGcaggctcag                                        >  1:653942/1‑150 (MQ=255)                                                                                                  tCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAATGTGCAGGCTCAGGCTGc                                   >  1:549180/1‑150 (MQ=255)                                                                                                           ggTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAATGTGCAGGCTCAGGCTGCAGGCATTgg                          <  2:769905/150‑1 (MQ=255)                                                                                                           ggTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAATGTGCAGGCTCAGGCTGCAGGCATTgg                          <  2:769994/150‑1 (MQ=255)                                                                                                              gAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGCATGTGCAGGCTCAGGCTGCAGGCATTGGCCa                       <  1:67583/150‑1 (MQ=255)                                                                                                                          ccACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAATGTGCAGGCTCAGGCTGCAGGCATTGGCCAGTGGCGCGCCTg           >  1:117101/1‑150 (MQ=255)                                                                                                                           cACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATCTCGGTATCTTCCGGCAGCGGAATGTGCAAGCTCAGGCTGCAGGCATTGGCCAGTGGCGAGCCTgc          >  2:308007/1‑150 (MQ=255)                                                                                                                                 gcggcgCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAATGTGCAGGCTCAGGCTGCAGGCATTGGCCAGTGGCGAGCCTGCGGCGGt    <  1:869828/150‑1 (MQ=255)                                                                                                                                 gcggcgCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAATGTGCAGGCTCAGGCTGCAGGCATTGGCCAGTGGCGAGCCTGCGGCGGt    <  1:287694/150‑1 (MQ=255)                                                                                                                                   ggcgCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAATGTGCAGGCTCAGGCTGCAGGCATTGGCCAGTGGCGAGCCTGCGGCGGTGa  <  1:760632/150‑1 (MQ=255)                                                                                                                                            |                                                                                                                                             ACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCTCAGTTGAGGTCGTCGTCCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGTCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAATGTGCAGGCTCAGGCTGCAGGCATTGGCCAGTGGCGAGCCTGCGGCGGTGA  >  NC_002947/1164506‑1164785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑