Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,844,126	Δ1 bp	coding (710/2754 nt)	gacS ←	sensor protein GacS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,844,125	0	G	.	100.0%	146.2 / NA	33	coding (711/2754 nt)	gacS	sensor protein GacS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (15/18);  total (15/18)

CTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGATGGGGTCGTTGATGGTGCGGCTCAT  >  NC_002947/1843978‑1844265                                                                                                                                                    |                                                                                                                                             cTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCgcc                                                                                                                                            >  2:144419/1‑148 (MQ=255)      tGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCg                                                                                                                                      >  1:312550/1‑150 (MQ=255)              gCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGc                                                                                                                              <  1:330475/150‑1 (MQ=255)                   aactGCCTCTTGGGGAGCAATATCGAGGTCGGCGTTCTGGATTGCGATGGTTTTCTGGTTCTGGCGCAGGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCACGCGGTCGTTGCGGTgg                                                                                                                         <  2:156873/147‑1 (MQ=255)                       gCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATg                                                                                                                               <  1:260980/140‑1 (MQ=255)                       gCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTGC‑GCCATGCGGTTGATg                                                                                                                               >  2:260980/1‑140 (MQ=255)                        ccTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAg                                                                                                                    <  2:129562/150‑1 (MQ=255)                         ctctTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGt                                                                                                                   >  2:515271/1‑150 (MQ=255)                                 gcgcCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAg                                                                                                           >  1:75815/1‑150 (MQ=255)                                 gcgcCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAg                                                                                                           <  2:558248/150‑1 (MQ=255)                                         tCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGc                                                                                                   >  2:576546/1‑150 (MQ=255)                                          ccAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTgg                                                                                                                         <  1:588523/127‑1 (MQ=255)                                          ccAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTgg                                                                                                                         >  2:588523/1‑127 (MQ=255)                                            aGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGc                                                                                                <  1:576546/150‑1 (MQ=255)                                                                    gTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGa                                                                        >  1:328421/1‑150 (MQ=255)                                                                    gTTTACAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGa                                                                        <  1:51625/150‑1 (MQ=255)                                                                         cAGGTTCTGGCGCACGTCTTCGGTTGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTgg                                                                   <  2:75815/150‑1 (MQ=255)                                                                                  gcgcACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGa                                                                        <  2:298693/136‑1 (MQ=255)                                                                                  gcgcACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCAAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGa                                                                        >  1:298693/1‑136 (MQ=255)                                                                                      aCGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTc                                                                                        >  1:52805/1‑116 (MQ=255)                                                                                      aCGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTc                                                                                        <  2:52805/116‑1 (MQ=255)                                                                                        gTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGa                                                    <  1:144419/150‑1 (MQ=255)                                                                                          cTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGc                                                                                                                       <  1:619452/81‑1 (MQ=255)                                                                                          cTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGc                                                                                                                       >  2:619452/1‑81 (MQ=255)                                                                                          cTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGAtt                                                  <  2:312550/150‑1 (MQ=255)                                                                                                       gTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTg                                     >  1:7473/1‑150 (MQ=255)                                                                                                        tCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGa                                    <  1:567185/150‑1 (MQ=255)                                                                                                          gATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATg                                  >  2:598685/1‑150 (MQ=255)                                                                                                                     gCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGATggg                       >  1:457526/1‑150 (MQ=255)                                                                                                                     gCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGATggg                       >  2:306752/1‑150 (MQ=255)                                                                                                                       aGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGATGGGGt                     >  1:145933/1‑150 (MQ=255)                                                                                                                               gTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGATGGGGTCGTTGATg             <  2:328421/150‑1 (MQ=255)                                                                                                                                 gggCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGATGGGGTCGTTGATGGt           <  1:598685/150‑1 (MQ=255)                                                                                                                                          gCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGATGGGGTCGTTGATGGTGCGGCTCAt  <  1:306752/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                             CTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGATGGGGTCGTTGATGGTGCGGCTCAT  >  NC_002947/1843978‑1844265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑