| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,705,459 | 0 | T | A | 35.3% | 8.7 / 13.9 | 17 | intergenic (‑151/+26) | kdpF/PP_4162 | potassium‑transporting ATPase subunit F/membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (6/5); new base A (4/2); total (10/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAAC > NC_002947/4705342‑4705608 | tttGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTAttgt > 2:26462/1‑150 (MQ=255) gggggATTTTGGCGGAGGGAGAGGGGTGAAGGGGGGATAGCGGGGGGGGGGGGTGGTTATAAAGAATGCGTAAAAGTCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTAttgttg < 1:168576/149‑1 (MQ=255) gatctaaTTGTCGGGGGGAGAGGGGTAAGGGGGCGAGATGGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATtgttgt < 2:513895/145‑1 (MQ=255) aTTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTtgtgagatc > 1:513895/1‑145 (MQ=255) tCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGgctgct > 1:561760/1‑150 (MQ=255) tCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGCGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGgctgct > 2:482525/1‑150 (MQ=255) aaGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCtc > 1:379147/1‑150 (MQ=255) gagaTCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCtccagtcca < 2:561760/150‑1 (MQ=255) cTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGcgcc < 2:597645/73‑1 (MQ=255) cTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGTACAGGCTTATTGTTGTGGCGGCTGCTGcgcc > 1:597645/1‑73 (MQ=255) cTGTAACGGCCCTTTCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGAt > 2:216136/1‑132 (MQ=37) cTGTAACGGCCCTTTCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGAt < 1:216136/132‑1 (MQ=37) gccctttCGCGGGCAAGCCCGCGAAGGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgcaa > 2:457924/6‑150 (MQ=39) ccctttCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGAt > 2:126053/5‑123 (MQ=37) ccctttCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGAt < 1:126053/119‑1 (MQ=37) tttCGCGGGCAAGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgcaagcaa > 1:543305/2‑150 (MQ=255) cgGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAgccgc < 1:482525/150‑1 (MQ=255) ttGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTc < 2:402626/102‑1 (MQ=255) ttGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTc > 1:402626/1‑102 (MQ=255) ttGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAac > 2:118027/1‑150 (MQ=255) | TTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAAC > NC_002947/4705342‑4705608 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |