Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,852,105	G→T	K383N (AAG→AAT)	relA →	ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,852,105	0	G	T	100.0%	110.6 / NA	35	K383N (AAG→AAT)	relA	ATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (16/19);  total (16/19)

TCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCG  >  NC_002947/1851969‑1852235                                                                                                                                         |                                                                                                                                   tCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAATATTTCCTGGTTGc                                                                                                                       <  1:147559/150‑1 (MQ=255)    gCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAATATTTCCTGGTTGCGTc                                                                                                                    <  1:457502/150‑1 (MQ=255)       ccGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAATATTTCCTGGTTGCGTCAgg                                                                                                                 <  2:436294/150‑1 (MQ=255)              cAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAATATTTCCTGGTTGCGTCAGGTGCTGGa                                                                                                          <  1:152241/150‑1 (MQ=255)                          ggTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGTGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAATATTTCCTGGTTGCGTCAGGTGCTGGa                                                                                                          <  1:490844/138‑1 (MQ=255)                          ggTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAATATTTCCTGGTTGCGTCAGGTGCTGGa                                                                                                          >  2:490844/1‑138 (MQ=255)                            tGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAATATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGc                                                                                            <  1:261994/150‑1 (MQ=255)                            tGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAATATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGc                                                                                            >  1:217601/1‑150 (MQ=255)                            tGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAATATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGc                                                                                            >  2:155686/1‑150 (MQ=255)                                     gTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCAAACCACTACGAAGAAAATATTAACTGGTTGGTTCATGTTCTGGAGTGTCACGAAGAGCTGGGCGACa                                                                                   >  2:573910/1‑150 (MQ=255)                                         ccACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAATATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCgg                                                                               >  2:482457/1‑150 (MQ=255)                                           aCGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAATATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGtg                                                                             <  2:625/150‑1 (MQ=255)                                             ggCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAATATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGtggc                                                                           <  2:217601/150‑1 (MQ=255)                                                aTGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAATATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGtggcctg                                                                        >  1:501208/1‑150 (MQ=255)                                                 tGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAATATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGtggcctgg                                                                       >  1:450005/1‑150 (MQ=255)                                                  gCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAATATTTcc                                                                                                                              >  1:585476/1‑94 (MQ=255)                                                  gCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAATATTTcc                                                                                                                              <  2:585476/94‑1 (MQ=255)                                                     cGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAATATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGa                                                                   <  1:131397/150‑1 (MQ=255)                                                         gagGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAATATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAg                                                               >  1:195340/1‑150 (MQ=255)                                                              cGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAATATTTCCTGGTTGc                                                                                                                       <  1:57322/89‑1 (MQ=255)                                                              cGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAATATTTCCTGGTTGc                                                                                                                       >  2:57322/1‑89 (MQ=255)                                                               gAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAATATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCgg                                                         <  1:155686/150‑1 (MQ=255)                                                                  cTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAATATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTc                                                      <  1:482457/150‑1 (MQ=255)                                                                           ggcgcCCACTGGCTCTAGAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAATATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAg                                             <  2:195340/149‑1 (MQ=255)                                                                                        gcTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAATATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGttt                                >  1:352427/1‑150 (MQ=255)                                                                                          tACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAATATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTAt                              <  2:501208/150‑1 (MQ=255)                                                                                                  cACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAATATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCAc                      <  1:620561/150‑1 (MQ=255)                                                                                                       aCGTCAAGCCCAGCTCCAACCACTACGAAGAAAATATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGACGACATCGAGCCTGACCAGGATTATGTGTTCAACCCCg                 >  1:371793/1‑150 (MQ=255)                                                                                                          tCAAGCCCAGCTCCAACCACTACGAAGAAAATATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGGCATCGAGCCTGACCGGTGTTATGTGTTCAcccccaaag              >  2:55277/1‑146 (MQ=255)                                                                                                                  aGCTCCAACCACTACGAAGAAAATATTTCCTGGTTGCGTCAGGTGCTg                                                                                                            <  2:441/48‑1 (MQ=255)                                                                                                                  aGCTCCAACCACTACGAAGAAAATATTTCCTGGTTGCGTCAGGTGCTg                                                                                                            >  1:441/1‑48 (MQ=255)                                                                                                                    cTCCAACCACTACGAAGAAAATATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATTTGTTCACCCCCGACGGCCACGCCAt    <  2:352427/150‑1 (MQ=255)                                                                                                                      ccAACCACTACGAAGAAAATATTTCCTGGTTGCGTCAGGTGCTGTAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCg  >  1:350472/1‑150 (MQ=255)                                                                                                                           cACTACGAAGAAAATATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTAt                              <  1:90971/117‑1 (MQ=255)                                                                                                                           cACTACGAAGAAAATATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTAt                              >  2:90971/1‑117 (MQ=255)                                                                                                                                         |                                                                                                                                   TCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCG  >  NC_002947/1851969‑1852235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑