Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,443,359	C→G	Q278H (CAG→CAC)	PP_1262 ←	LysR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,443,359	0	C	G	100.0%	167.1 / NA	50	Q278H (CAG→CAC)	PP_1262	LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (26/24);  total (26/24)

TACCATTGTCCGCCCCCGCATTGCAGCAGCTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATCGCA  >  NC_002947/1443212‑1443500                                                                                                                                                    |                                                                                                                                              tACCATTGTCCGCCCCCGCATTGCAGCAGCTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTg                                                                                                                                             >  1:179306/1‑150 (MQ=255) tACCATTGTCCGCCCCCGCATTGCAGCAGCTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTg                                                                                                                                             <  2:563500/150‑1 (MQ=255)  aCCATTGTCCGCCCCCGCATTGCAGCAGCTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGc                                                                                                                                            >  1:578267/1‑150 (MQ=255)        gTCCGCCCCCGCATTGCAGCAGCTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGAt                                                                                                                                      <  2:584254/150‑1 (MQ=255)           cGCCCCCGCATTGCAGCAGCTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGa                                                                                                                                   <  1:284441/150‑1 (MQ=255)                ccGCATTGCAGCAGCTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGAt                                                                                                                              <  2:134434/150‑1 (MQ=255)                ccGCATTGAAGCAGCTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCATCTGGGTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGAt                                                                                                                              >  1:469826/1‑150 (MQ=255)                            agcTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGtt                                                                                                                  <  1:605041/150‑1 (MQ=255)                             gcTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTc                                                                                                                 <  1:142198/150‑1 (MQ=255)                                           gATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCg                                                                                                   >  1:1155039/1‑150 (MQ=255)                                            aTCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGa                                                                                                  >  1:63852/1‑150 (MQ=255)                                                     tGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGc                                                                                         >  2:490346/1‑150 (MQ=255)                                                         gggTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATg                                                                                                                                     >  1:127452/1‑102 (MQ=255)                                                         gggTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATg                                                                                                                                     <  2:127452/102‑1 (MQ=255)                                                            ttttCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTAccc                                                                                  >  1:381235/1‑150 (MQ=255)                                                                cAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCTGTAc                                                                              <  1:490346/150‑1 (MQ=255)                                                                       gCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTa                                                                       <  1:858722/150‑1 (MQ=255)                                                                             tGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTcc                                                                 >  1:1319335/1‑150 (MQ=255)                                                                                  ggCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAg                                                            <  2:381235/150‑1 (MQ=255)                                                                                   gCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAgg                                                           >  1:1325793/1‑150 (MQ=255)                                                                                     aaGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGTTACCGTCCCGCAGGcc                                                         >  2:813011/1‑150 (MQ=255)                                                                                       caaTCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCt                                                       >  2:1026146/2‑150 (MQ=255)                                                                                          tctcTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGGCCCGCAGGCCCTCGa                                                    >  2:1040185/1‑150 (MQ=255)                                                                                             ctGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAg                                                 <  2:63852/150‑1 (MQ=255)                                                                                                gCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAg                                                                           <  2:626656/121‑1 (MQ=255)                                                                                                gCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAg                                                                           >  1:626656/1‑121 (MQ=255)                                                                                                 cAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGa                                             <  2:1325793/150‑1 (MQ=255)                                                                                                   gCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAg                                                                                                                     <  2:498263/76‑1 (MQ=255)                                                                                                   gCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAg                                                                                                                     >  1:498263/1‑76 (MQ=255)                                                                                                   gCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAg                                                                           >  2:599960/1‑118 (MQ=255)                                                                                                   gCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAg                                                                           <  1:599960/118‑1 (MQ=255)                                                                                                   gCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAgg                                                           <  2:789534/134‑1 (MQ=255)                                                                                                   gCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAgg                                                           >  1:789534/1‑134 (MQ=255)                                                                                                          gcgcAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCgg                                    <  2:1257632/150‑1 (MQ=255)                                                                                                                gTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCg                                     >  1:148117/1‑143 (MQ=255)                                                                                                                gTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCg                                     <  2:148117/143‑1 (MQ=255)                                                                                                                gTACTCGACCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCAc                              >  2:1239036/1‑150 (MQ=255)                                                                                                                       aCCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTAccc                                                                                  >  2:982658/1‑91 (MQ=255)                                                                                                                       aCCCAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTAccc                                                                                  <  1:982658/91‑1 (MQ=255)                                                                                                                        cccAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGTTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGc                      <  1:813011/150‑1 (MQ=255)                                                                                                                         ccAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAgg                                                                          <  1:330293/97‑1 (MQ=255)                                                                                                                         ccAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAgg                                                                          >  2:330293/1‑97 (MQ=255)                                                                                                                         ccAGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCAGTACCAGGCTACCGTGCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGcc                     >  2:697569/1‑150 (MQ=255)                                                                                                                           aGGTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCa                   >  2:983483/1‑150 (MQ=255)                                                                                                                            ggTCTTGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCAt                  <  2:1155039/150‑1 (MQ=255)                                                                                                                                ttGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGaa                                            <  1:378097/120‑1 (MQ=255)                                                                                                                                ttGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGaa                                            >  2:378097/1‑120 (MQ=255)                                                                                                                                ttGATCTTGGCATCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCa              >  1:702245/1‑150 (MQ=255)                                                                                                                                           aTCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATCGc   >  2:242690/1‑150 (MQ=255)                                                                                                                                            tCCAGATAGTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATCGCa  <  2:702245/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                              TACCATTGTCCGCCCCCGCATTGCAGCAGCTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAAGAATCTCTGGCAGCGAGTTGCGCAGGTACTCGACCCAGGTCTTGATCTTGGCATCCAGATACTGCCGCGATGGATAGATGGCATACAGGTTCAACTCCTGCAGGCGATACTCCGGCAGTACCCGTACCAGGCTACCGTCCCGCAGGCCCTCGATAGCCGAATAGATCGGCAGCACGCCTACGCCCATGCCACTGCGGATCGCA  >  NC_002947/1443212‑1443500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑