Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,851,537 C→T A194V (GCG→GTG)  relA → ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,851,5370CT95.6% 134.3 / ‑2.1 45A194V (GCG→GTG) relAATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (16/27);  total (17/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.13e-01

AAGATGCTGGTGGCCATGGTCGACGACGTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAG  >  NC_002947/1851398‑1851678
                                                                                                                                            |                                                                                                                                             
aaGATGCTGGTGGCCATGGTCGACGACGTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTcgcg                                                                                                                                     >  1:1309837/1‑150 (MQ=255)
          tggCCATGGTCGACGACGTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTgg                                                                                                                           <  1:901488/150‑1 (MQ=255)
              cATGGTCGACGACGTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTAt                                                                                                                       >  1:1090416/1‑150 (MQ=255)
              cATGGTCGACGACGTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTAt                                                                                                                       >  1:1213286/1‑150 (MQ=255)
                     gacgacGTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATcac                                                                                                                >  1:264098/1‑150 (MQ=255)
                        gacgTGCGCGTGCCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATc                                                                                                             >  2:1112590/1‑150 (MQ=255)
                            tGCGCGTGGCGCTGATCAAGCTGGCCGAACGTACGTACGCAATCCGGGCGGTCAAGTCCGCCGATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGt                                                                                                         <  2:611364/150‑1 (MQ=255)
                            tGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGt                                                                                                         <  2:264098/150‑1 (MQ=255)
                            tGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGt                                                                                                         <  2:1385328/150‑1 (MQ=255)
                             gcgcGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTg                                                                                                        <  1:1293770/150‑1 (MQ=255)
                              cgcgTGGCGCTGATCAAGCTGGTCGAACGCACGTGCGCAATTCGGGCGGTCAAGTCCGCCGATGA‑CGATAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTgg                                                                                                       <  1:583802/150‑1 (MQ=255)
                                 gTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAg                                                                                                    >  2:587586/1‑150 (MQ=255)
                                          aTCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGAc                                                                                           >  2:1269646/1‑150 (MQ=255)
                                          aTCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGAc                                                                                           <  2:1213286/150‑1 (MQ=255)
                                              aGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGt                                                                                       <  2:1309837/150‑1 (MQ=255)
                                                   gCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTgg                                                                                                                           <  2:95557/109‑1 (MQ=255)
                                                   gCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTgg                                                                                                                           >  1:95557/1‑109 (MQ=255)
                                                   gCCGAACGAACGTGCGAACTCTGGGCGGTCAAGTCCGCCGAACA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTc                                                                                  <  2:77772/150‑1 (MQ=255)
                                                         cGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTAc                                                                            <  2:1090416/150‑1 (MQ=255)
                                                               tGCGCAATCCGGGCGGTCAAGTCCGCCGATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTc                                                                                                                                        >  2:559722/1‑84 (MQ=255)
                                                               tGCGCAATCCGGGCGGTCAAGTCCGCCGATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTc                                                                                                                                        <  1:559722/84‑1 (MQ=255)
                                                                  gcAATCCGGGCGGTCAAGTCCGCCGATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAg                                                                                                    >  2:1373943/1‑117 (MQ=255)
                                                                  gcAATCCGGGCGGTCAAGTCCGCCGATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAg                                                                                                    <  1:1373943/117‑1 (MQ=255)
                                                                       ccGGGCGGTCAAGTCCGCCGATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCa                                                              <  1:1082359/150‑1 (MQ=255)
                                                                       ccGGGCGGGCAAGTCCG‑CGCCGACCAACAAGCGCCTCCGCGTCGCGCGTGAAGTGTTCGACATCTATGTCCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCa                                                              <  1:26686/150‑1 (MQ=255)
                                                                               tCAAGTCCGCCGATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTCTCGATCAAATCAAGTGGGAGCTGGAAGACCTGTCCTTCCTCTACCTGGATCCCGACCACTACAAGc                                                      >  2:50228/1‑150 (MQ=255)
                                                                               tCAAGTCCGCCGATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTgg                                                                                                       >  2:96681/1‑101 (MQ=255)
                                                                               tCAAGTCCGCCGATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTgg                                                                                                       <  1:96681/101‑1 (MQ=255)
                                                                               tCAAGTCCGCCGATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGc                                                      <  1:1269646/150‑1 (MQ=255)
                                                                               acttgaccgccgATGA‑CGAGAAACGCCCGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGc                                                      <  2:1369148/144‑1 (MQ=255)
                                                                                    tCCGCCGATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATc                                                 >  1:1309536/1‑150 (MQ=255)
                                                                                          gATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGATGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAg                                           <  2:284481/150‑1 (MQ=255)
                                                                                            tGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCt                                         <  1:1007668/150‑1 (MQ=255)
                                                                                              a‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCa                                                              <  2:331553/127‑1 (MQ=255)
                                                                                              a‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCa                                                              >  1:331553/1‑127 (MQ=255)
                                                                                                cGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGtt                                      <  1:433102/150‑1 (MQ=255)
                                                                                                cGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGtt                                      <  2:1316970/150‑1 (MQ=255)
                                                                                                cGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGtt                                      <  2:1309536/150‑1 (MQ=255)
                                                                                                 gagaAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAg                                                                      <  2:493089/117‑1 (MQ=255)
                                                                                                 gagaAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAg                                                                      >  1:493089/1‑117 (MQ=255)
                                                                                                 gagaAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTg                                     >  1:996224/1‑150 (MQ=255)
                                                                                                                        tGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCTTTCGCGTAAGAGGAGGTATACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGa              >  1:551037/1‑150 (MQ=255)
                                                                                                                        tGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGa              <  1:691048/150‑1 (MQ=255)
                                                                                                                                    cATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCACGAAATAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAg  <  1:161865/150‑1 (MQ=255)
                                                                                                                                    cATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAg  <  1:587586/150‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                             
AAGATGCTGGTGGCCATGGTCGACGACGTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGA‑CGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAG  >  NC_002947/1851398‑1851678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑