Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,800,263	Δ1 bp	7.0%	coding (220/471 nt)	PP_4227 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,800,263	0	G	.	7.0%	171.5 / 7.9	57	coding (220/471 nt)	PP_4227	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (26/27);  new base . (2/2);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

CGTCCATGACGCCATCGTGCTTGTGGTTGCTGGCGAGGATCTCGCCACGCAGCGAGCTGACCTGACAGGCCATGCCATCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAACATCATCTGCTGGCGCAGGTCACGCAGCATCCACGCCGCGGCCAGCGCCCAGATGATCACGAAGGCGCTGTCGAGGATCAGGCTCAGGCG  >  NC_002947/4800116‑4800410                                                                                                                                                    |                                                                                                                                                    cGTCCATGACGCCATCGTGCTTGTGGTTGCTGGCGAGGATCTCGCCACGCAGCGAGCTGACCTGACAGGCCATGCCATCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGt                                                                                                                                                   <  2:298963/150‑1 (MQ=255)    ccATGACGCCATCGTGCTTGTGGTTGCTGGCGAGGATCTCGCCACGCAGCGAGCTGACCTGACAGGCCATGCCATCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGa                                                                                                                                                <  1:850389/150‑1 (MQ=255)              aTCGTGCGTGTGGTTGCTGGCGAGGATCTCGCCACGCAGCGAGCTGACCTGACAGGCCATGCCATCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGc                                                                                                                                      <  2:121870/150‑1 (MQ=255)                cGTGCTTGTGGTTGCTGGCGAGGATCTCGCCACGCAGCGAGCTGACCTGACAGGCCATGCCATCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGa                                                                                                                                    <  1:1353920/150‑1 (MQ=255)                            tGCTGGCGAGGATCTCGCCACGCAGCGAGCTGACCTGACAGGCCATGCCATCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGc                                                                                                                                           <  2:714030/131‑1 (MQ=255)                            tGCTGGCGAGGATCTCGCCACGCAGCGAGCTGACCTGACAGGCCATGCCATCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGc                                                                                                                                           >  1:714030/1‑131 (MQ=255)                            tGCTGGCGAGGATCTCGCCACGCAGCGAGCTGACCTGACAGGCCATGCCATCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCg                                                                                                                        >  1:302464/1‑150 (MQ=39)                                    aGGATCTCGCCACGCAGCGAGCTGACCTGACAGGCCATGCCATCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCAGACGCGCCGACgccgcc                                                                                                                >  2:974717/1‑150 (MQ=255)                                      gATCTCGCCACGCAGCGAGCTGACCTGACAGGCCATGCCATCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAg                                                                                                              >  1:138615/1‑150 (MQ=255)                                      gATCTCGCCACGCAGCGAGCTGACCTGACAGGCCATGCCATCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAg                                                                                                              <  1:905764/150‑1 (MQ=255)                                      gATCTCGCCACGCAGCGAGCTGACCTGACAGGCCACGCCATCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAg                                                                                                              >  2:1919989/1‑150 (MQ=255)                                        tctcGCCACGCAGCGAGCTGACCTGACAGGCCATGCCATCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGgc                                                                                                            <  1:814488/150‑1 (MQ=255)                                            gCCACGCAGCGAGCTGACCTGACAGGCCATGCCATCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACCGGTCGATCAGACCGGCGACCATACGCGCCGACGCCGCCCGGCGCTg                                                                                                        >  2:401592/1‑150 (MQ=255)                                               ccGCAGCGAGCTGACCCGACAGGCCATGCCAGCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTc                                                                                                     <  2:1885111/149‑1 (MQ=255)                                                  cAGCGAGCTGACCTGACAGGCCATGCCATCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGACGACGCCGCCAGGCGCTTGTCGAg                                                                                                  <  2:882066/150‑1 (MQ=255)                                                   aGCGAGCTGACCTGACAGGCCATGCCATCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAgg                                                                                                 >  1:601950/1‑150 (MQ=255)                                                   aGCGAGCTGACCTGACAGGCCATGCCATCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAgg                                                                                                 <  1:1044231/150‑1 (MQ=255)                                                       aGCTGACCTGACAGGCCATGCCATCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGgaga                                                                                             >  2:260992/1‑150 (MQ=255)                                                          tgacctgacAGGCCATGCCATCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACT‑GTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGa                                                                                                   <  2:1591548/140‑1 (MQ=255)                                                          tgacctgacAGGCCATGCCATCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACT‑GTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGa                                                                                                   <  2:1592361/140‑1 (MQ=255)                                                          tgacctgacAGGCCATGCCATCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACT‑GTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGa                                                                                                   >  1:1592361/1‑140 (MQ=255)                                                          tgacctgacAGGCCATGCCATCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACT‑GTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGa                                                                                                   >  1:1591548/1‑140 (MQ=255)                                                            acctgacAGGCCATGCCATCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAAcatc                                                                                        >  1:1228914/1‑150 (MQ=255)                                                               tgacAGGCCATGCCATCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAACatcatc                                                                                     >  2:749148/1‑150 (MQ=255)                                                               tgacAGGCCATGCCATCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAACatcatc                                                                                     >  1:1391090/1‑150 (MQ=255)                                                                          gccatCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAACATCATCTGCTGGCGCAg                                                                          >  1:757808/1‑150 (MQ=255)                                                                             atCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAgg                                                                                                 >  1:356663/1‑124 (MQ=255)                                                                             atCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAgg                                                                                                 <  2:356663/124‑1 (MQ=255)                                                                                 ggTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAAca                                                                                          <  1:260576/127‑1 (MQ=255)                                                                                 ggTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAAca                                                                                          >  2:260576/1‑127 (MQ=255)                                                                                  gTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAACATCATCTGCTGGCGCAGGTCACgca                                                                  <  2:601950/150‑1 (MQ=255)                                                                                  gTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAACATCATCTGCTGGCGCAGGTCACgca                                                                  <  2:1323730/150‑1 (MQ=255)                                                                                  gTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAACATCATCTGCTGGCGCAGGTCACgca                                                                  >  2:1777777/1‑150 (MQ=255)                                                                                    aCGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAACATCATCTGCTGGCGCAGGTCACgcagc                                                                <  1:726789/150‑1 (MQ=255)                                                                                      gCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAAc                                                                                           <  1:688193/121‑1 (MQ=255)                                                                                      gCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAAc                                                                                           >  2:688193/1‑121 (MQ=255)                                                                                      gCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAACATCATCTGCTGGCGCAGGTCACGCAGCAt                                                              >  2:1869732/1‑150 (MQ=255)                                                                                      gCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGACGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAACATCATCTGCTGGCGCAGGTCACGCAGCAt                                                              >  1:241346/1‑150 (MQ=255)                                                                                             tGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAACATCATCTGCTGGCGCAGGTCACGCAGCATCCAcgcc                                                       >  1:1464702/1‑150 (MQ=255)                                                                                                tCGGCGGAAAAATGCGCCTCCCCGAAATAACCTGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAACATCATCTGCTGGCGCAGGTCACGCAGCATCCACGCcgcg                                                    >  1:988786/1‑150 (MQ=17)                                                                                                  ggcggAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAACATCATCTGCTGGAGCAGGTCACGCAGCATCCACGCCGCGGc                                                  <  1:730793/150‑1 (MQ=255)                                                                                                     ggAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAACATCATCTGCTGGCGCAGGTCACGCAGCATCCACGCCGCGGCCAg                                               <  2:94617/150‑1 (MQ=255)                                                                                                     ggAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAACATCATCTGCTGGCGCAGGTCACGCAGCATCCACGCCGCGGCCAg                                               <  2:87402/150‑1 (MQ=255)                                                                                                      gAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAACATCATCTGCTGGCGCAGGTCACGCAGCATCCACGCCGCGGCCAgc                                              >  1:1464454/1‑150 (MQ=255)                                                                                                       aaaaaTGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAACATCATCTGCTGGCGCAGGTCACGCAGCATCCACGCCGCGGCCAgcg                                             >  1:1270285/1‑150 (MQ=255)                                                                                                             gcgcCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAACATCATCTGCTGGCGCAGGTCACGCAGCATCCACGCCGCGGCCAGCGCCCAga                                       >  1:1308465/1‑150 (MQ=255)                                                                                                               gcCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAACATCATCTGCTGGCGCAGGTCACGCAGCATCCACGCCGCGGCCAGCGCCCAgatg                                     >  1:201142/1‑150 (MQ=255)                                                                                                                       gCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAACATCATCTGCTGGCGCAGGTCACGCAGCATCCACGCCGCGGCCAGCGCCCAGATGATCACGaa                             <  1:749148/150‑1 (MQ=255)                                                                                                                         ccTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAACATCATCTGCTGGCGCAGGTCACGCAGCATCCACGCCGCGGCCAGCGCCCAGATGATCACGAAgg                           <  2:670524/150‑1 (MQ=255)                                                                                                                               cGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAACATCATCTGCTGGCGCAGGTCACGCAGCATCCACGCCGCGGCCAGCGCCCAGATGATCACGAAGGCGCTGt                     <  2:1270285/150‑1 (MQ=255)                                                                                                                                ggTCAGCGGCTGCGCCAATTGGTCTATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAACATCATCTGCTGGCGCAGGTCACGCAGCATCCACGCCGCGGCCAGCGCCCAGATGATCACGAAGGCGCTGTc                    <  2:108919/150‑1 (MQ=255)                                                                                                                                  tCAGCGGCTGCGGCAACTGGTCGATCAGCCCAGCCACCCTCCAAATACAGACTGACATGCGCTGGTCGAGGGAGAACATCATCTGCTGGCGCAGGTCACGCAGCATCCACGCCGCGGCCAGCGCCCAGATGATCACGAAGGCGCTGTCGa                  <  2:988786/150‑1 (MQ=17)                                                                                                                                   cAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAACATCATCTGCTGGCGCAGGTCACGCAGCATCCACGCCGCGGCCAGCGCCCAGATGATCACGAAGGCGCTGTCGAg                 <  2:1308465/150‑1 (MQ=255)                                                                                                                                       ggCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAACATCATCTGCTGGCGCAGGTCACGCAGCATCCACGCCGCGGCCAGCGCCCAGATGATCACGAAGGCGCTGTCGAGGAtc             >  2:999747/1‑150 (MQ=255)                                                                                                                                           gCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAACATCATCTGCTGGCGCAGGTCACGCAGCATCCACGCCGCGGCCAGCGCCCAGATGATCACGAAGGCGCTGTCGAGGAtcaggc         >  1:1575618/1‑150 (MQ=255)                                                                                                                                              gCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAACATCATCTGCTGGCGCAGGTCACGCAGCATCCACGCCGCGGCCAGCGCCCAGATGATCACGAAGGCGCTGTCGAGGAtcaggctca      <  1:922614/150‑1 (MQ=255)                                                                                                                                                  cTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAACATCATCTGCTGGCGCAGGTCACGCAGCATCCACGCCGCGGCCAGCGCCCAGATGATCACGAAGGCGCTGTCGAGGATCAGGCTCAGGCg  <  1:1647423/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CGTCCATGACGCCATCGTGCTTGTGGTTGCTGGCGAGGATCTCGCCACGCAGCGAGCTGACCTGACAGGCCATGCCATCCGGTACGCTGAACTGGTCGGCGGAAAAATGCGCCTCCCCGCCCTTGGCGGTCAGCGGCTGCGGCAACTGGTCGATCAGCCCGGCGACCATACGCGCCGACGCCGCCAGGCGCTGGTCGAGGGAGAACATCATCTGCTGGCGCAGGTCACGCAGCATCCACGCCGCGGCCAGCGCCCAGATGATCACGAAGGCGCTGTCGAGGATCAGGCTCAGGCG  >  NC_002947/4800116‑4800410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑