Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	163,968:1	+T	5.6%	intergenic (‑135/+52)	scpC ← / ← pntB	propionyl‑CoA:succinate CoA transferase/pyridine nucleotide transhydrogenase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	163,968	1	.	T	5.6%	288.9 / 9.8	72	intergenic (‑135/+52)	scpC/pntB	propionyl‑CoA:succinate CoA transferase/pyridine nucleotide transhydrogenase subunit beta
Reads supporting (aligned to +/- strand):  ref base . (35/33);  new base T (2/2);  total (37/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

GATCACGGTACATGGATTGTTATCTCGGGCTACTGAAACTACTGCAGCGCAGTCTAGAGATTTCGCCCATTGCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTGGCGTCACCGAACACCATCATGGTCTTGTCCAGATAGAACAGTTCGTTGTCCAGGCCGG  >  NC_002947/163821‑164117                                                                                                                                                     |                                                                                                                                                      gATCACTGTACATGGATTGTTATCTCGGGCTACTGAAACTACTGCAGCGCAGTCTAGAGATTTCGCCCATTGCCGTCCCACGACCATGGTCGCATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑Ca                                                                                                                                                     >  2:75476/1‑150 (MQ=255)     aCGGTACATGGATTGTTATCTCGGGCTACTGAAACTACTGCAGCGCAGTCAAGAGATTTCGCCGATTGCCGGCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAACCCCGG‑CAGAtt                                                                                                                                                 >  2:1056352/1‑150 (MQ=255)            aTGGATTGTTATCTCGGGCTACTGAAACTACTGCAGCGCAGTCTAGAGATTTCGCCCATTGCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCg                                                                                                                                          <  1:1900312/150‑1 (MQ=255)               gATTGTTATCTCGGGCTACTGAAACTACTGCAGCGCAGTCTAGAGATTTCGCCCATTGCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCgggg                                                                                                                                       <  1:256828/150‑1 (MQ=255)                    ttATCTCGGGCTACTGAAACTACTGCAGCGCAGTCTAGAGATTTCGCCCATTGCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGa                                                                                                                                             >  2:1390901/1‑139 (MQ=255)                    ttATCTCGGGCTACTGAAACTACTGCAGCGCAGTCTAGAGATTTCGCCCATTGCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGa                                                                                                                                             <  1:1390901/139‑1 (MQ=255)                       tctcGGGCTACTGAAACTACTGCATCGCAGTCTAGAGATTTCGCCCATTGCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCttt                                                                                                                               >  2:1785988/1‑150 (MQ=255)                        ctcGGGCTACTGAAACTACTGCAGCGCAGTCTAGAGATTTCGCCCATTGCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGGTCAGAATCTG‑CCGGGGCttttcttt                                                                                                                              <  2:1876403/150‑7 (MQ=255)                         tcGGGCTACTGAAACTACTGCAGCGCAGTCTAGAGATTTCGCCCATTGCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTtat                                                                                                                             <  1:406686/150‑1 (MQ=255)                               tACTGAAACTACTGCAGCGCAGTCTAGAGATTTCGCCCATTGCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGc                                                                                                                       <  1:468350/150‑1 (MQ=255)                               tACTGAAACTACTGCAGCGCAGTCTAGAGATTTCGCCCATTGCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGc                                                                                                                       >  2:178520/1‑150 (MQ=255)                                 cTGAAACTACTGCAGCGCAGTCTAGAGATTTCGCCCATTGCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGCTCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTg                                                                                                                     <  2:1324106/150‑1 (MQ=255)                                 cTGAAACTACTGCAGCGCAGTCTAGAGATTTCGCCCATTGCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTg                                                                                                                     >  2:1693532/1‑150 (MQ=255)                                   gAAACTACTGCAGCGCAGTCTAGAGATTTCGCCCATTGCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGt                                                                                                                   >  2:844201/1‑150 (MQ=255)                                         actGCAGCGCAGTCTAGAGATTTCGCCCATTGCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAg                                                                                                             <  1:1700845/150‑1 (MQ=255)                                            gCAGCGCAGTCTAGAGATTTCGCCCATTGCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCCC‑CAGACTCTGACTTGTTCTTCCTTTATATCAGCTGGTTTGCAGCgg                                                                                                          <  1:832890/150‑1 (MQ=255)                                               gcgcAGTCTAGAGATTTCGCCCATTTCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGc                                                                                                            <  1:1463221/145‑1 (MQ=255)                                               gcgcAGTCTAGAGATTTCGCCCATTTCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGc                                                                                                            >  2:1463221/1‑145 (MQ=255)                                                 gcAGTCTAGAGATTTCGCCCATTGCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGgt                                                                                                     <  1:92057/150‑1 (MQ=255)                                                 gcAGTCTAGAGATTTCGCCCATTGCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGgt                                                                                                     <  2:63806/150‑1 (MQ=255)                                                     tCTAGAGATTTCGCCCATTGCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCa                                                                                                 >  1:1509623/1‑150 (MQ=255)                                                     tCTAGAGATTTCGCCCATTGCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCa                                                                                                 >  1:1022722/1‑150 (MQ=255)                                                       tAGAGATTTCGCCCATTGCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGGTCAGAATCTG‑CCGGGGCttttctt                                                                                                                               <  2:1188056/118‑6 (MQ=38)                                                       tAGAGATTTCGCCCATTGCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGGTCAGAATCTG‑CCGGGGCttttctt                                                                                                                               >  1:1188056/1‑113 (MQ=38)                                                       tAGAGATTTCGCCCATTGCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACt                                                                                               >  1:1320389/1‑150 (MQ=255)                                                        agagATTTCGCCCATTGCCGTCCCACGACCATGGTAGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTc                                                                                              >  1:410333/1‑150 (MQ=255)                                                         gagaTTTCGCCCATTGCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTcc                                                                                             >  2:153770/1‑150 (MQ=255)                                                         cccaagtCGCCCATTGCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTcc                                                                                             >  2:1472644/7‑150 (MQ=255)                                                                 gCCCATTGCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGc                                                                                                                       >  1:922913/1‑116 (MQ=255)                                                                 gCCCATTGCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGc                                                                                                                       <  2:922913/116‑1 (MQ=255)                                                                 gCCCATTGCCGTCCCACGACCATGGTAGTGTGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCtt                                                                                     >  1:529294/1‑150 (MQ=255)                                                                   ccATTGCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGa                                                                                   >  2:314869/1‑150 (MQ=255)                                                                     aTTGCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGAcc                                                                                 <  1:1693532/150‑1 (MQ=255)                                                                         ccGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCAATTATACGAGCTGGTTTTCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGt                                                                             >  1:1563271/1‑150 (MQ=255)                                                                                aCGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGAt                                                                      >  1:825561/1‑150 (MQ=255)                                                                                  gACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGa                                                                    >  1:992255/1‑150 (MQ=255)                                                                                   aCCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGAc                                                                   <  1:314869/150‑1 (MQ=255)                                                                                    ccATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATCCGAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGAcc                                                                  <  1:117036/150‑1 (MQ=255)                                                                                    ccATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGAcc                                                                  >  2:428027/1‑150 (MQ=255)                                                                                         gTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACcttctt                                                             <  1:1785988/150‑1 (MQ=255)                                                                                          tCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTg                                                            <  1:428027/150‑1 (MQ=255)                                                                                          tCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTg                                                            >  1:1487814/1‑150 (MQ=255)                                                                                            gTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTc                                                                                              >  1:1257271/1‑114 (MQ=255)                                                                                            gTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTc                                                                                              <  2:1257271/114‑1 (MQ=255)                                                                                                gagaAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCAG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTGGCGTCa                                                      <  2:529294/150‑1 (MQ=255)                                                                                                  gaAGCCTTGTATTTGAGTCGTTGATCCGGATCAATCACTAAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTGGCGTCAcc                                                    <  1:99352/150‑1 (MQ=255)                                                                                                  gaAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCt                                                                          <  2:1334458/128‑1 (MQ=255)                                                                                                  gaAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCt                                                                          >  1:1334458/1‑128 (MQ=255)                                                                                                   aaGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTGGCGTCACCg                                                   <  1:153770/150‑1 (MQ=255)                                                                                                      ccTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTGGCGTCACCGAac                                                <  1:1518812/150‑1 (MQ=255)                                                                                                             tttGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTg                                                            >  2:1338505/1‑131 (MQ=255)                                                                                                             tttGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTg                                                            <  1:1338505/131‑1 (MQ=255)                                                                                                             tttGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTGGCGTCACCGAACACcatca                                         >  1:1121197/1‑150 (MQ=255)                                                                                                             tttGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTGGCGTCACCGAACACcatca                                         <  1:407917/150‑1 (MQ=255)                                                                                                                   tCGTTGATCCGGATCAATAAAAGACAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTGGCGTCACCGAACACCATCATGGTCt                                   >  2:1284346/1‑150 (MQ=255)                                                                                                                     gTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTGGCGTCACCGAACACCATCATGGTCTTg                                 <  1:46831/150‑1 (MQ=255)                                                                                                                      ttGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTGGCGTCACCGAACACCATCATGGTCTTGt                                <  1:844201/150‑1 (MQ=255)                                                                                                                      ttGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTGGCGTCACCGAACACCATCATGGTCTTGt                                <  2:483656/150‑1 (MQ=255)                                                                                                                         aTCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTGGCGTCACCGAACACCATCATGGTCTTGTCCa                             >  1:267238/1‑150 (MQ=255)                                                                                                                            cGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTGGCGTCACCGAACACCATCATGGTCTTGTCCAGAt                          <  2:1509623/150‑1 (MQ=255)                                                                                                                            cGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTGGCGTCACCGAACACCATCATGGTCTTGTCCAGAt                          <  2:410333/150‑1 (MQ=255)                                                                                                                             ggATCAATAGAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTGGCGTCACCGAACACCATCATGGTCTTGTCCAGATa                         <  2:1661388/150‑1 (MQ=255)                                                                                                                                 cAATAAAAGAAAAGCCCCGG‑CAGATACTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTGGCGTCACCGAACACCATCATGGTCTTGTCCAGATAGAAc                     >  2:643983/1‑150 (MQ=255)                                                                                                                                   aTAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTGGCGTCACCGAACACCATCATGGTCTTGTCCAGATCGAACAg                   >  2:306596/1‑150 (MQ=255)                                                                                                                                    tAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTGGCGTCACCGAACACCATCATGGTCTTGTCCAGATAGAACAGt                  >  2:141378/1‑150 (MQ=255)                                                                                                                                    tAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTGGCGTCACCGAACACCATCATGGTCTTGTCCAGATAGAACAGt                  >  2:1830298/1‑150 (MQ=255)                                                                                                                                    tAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCGTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTGGCGTCACCGAACACCATCATGGTCTTGTCCAGATAGAACAGt                  >  2:55136/1‑150 (MQ=255)                                                                                                                                     aaaagaaaagCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCCGCTGGTGTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTGGCGTCACCGAACACCATCATGGTCTTGTCCAGATAGAACAGtt                 <  1:1056352/150‑1 (MQ=255)                                                                                                                                     aaaagaaaagCCCCGG‑CAGATTCTGACCGGGGCTTCCTGTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTGGCGTCACCGAACACCATCATGGTCTTGTCCAGATAGAACAGtt                 >  2:1914659/1‑150 (MQ=255)                                                                                                                                         gaaaagCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTGGCGTCACCGAACACCATCATGGTCTTGTCCAGATAGAACAGTTCGtt             <  1:75476/150‑1 (MQ=255)                                                                                                                                               ccccGGTCAGAATCTG‑CCGGGGCTTCCTTTATATCAGCAGGTTTGCTGCTACTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTGGCGTCACCGAACACCATCATGGTCTTGTCCAGATAGAACAGTTCGTTGTCCAg       >  1:1281049/1‑150 (MQ=255)                                                                                                                                                  cGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTGGCGTCACCGAACACCATCATGGTCTTGTCCAGATAGAACAGTTCGTTGTCCAGGcc    >  2:402113/1‑150 (MQ=255)                                                                                                                                                    g‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTGGCGTCACCGAACACCATCATGGTCTTGTCCAGATAGAACAGTTCGTTGTCCAGGCCgg  <  2:1487814/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                      GATCACGGTACATGGATTGTTATCTCGGGCTACTGAAACTACTGCAGCGCAGTCTAGAGATTTCGCCCATTGCCGTCCCACGACCATGGTCGTATGAGAAGCCTTGTATTTGAGTCGTTGATCCGGATCAATAAAAGAAAAGCCCCGG‑CAGATTCTGACCGGGGCTTCCTTTATATCAGCTGGTTTGCAGCGGCTGGTGTCACTCCACCGCCTTGACCATGTCCTCGATGACCTTCTTGGCGTCACCGAACACCATCATGGTCTTGTCCAGATAGAACAGTTCGTTGTCCAGGCCGG  >  NC_002947/163821‑164117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑