Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,295,031:1 +T 6.4% intergenic (+41/+29) slyB → / ← nhaA‑I outer membrane lipoprotein/Na(+)/H(+) antiporter NhaA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,295,0311.T 6.4% 307.3 / 12.9 78intergenic (+41/+29)slyB/nhaA‑Iouter membrane lipoprotein/Na(+)/H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base . (37/36);  new base T (3/2);  total (40/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

TGGCAGCATGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCGGCGTACTCACTGCTACCCGGG  >  NC_002947/1294882‑1295180
                                                                                                                                                      |                                                                                                                                                     
tgGCAGCATGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCa                                                                                                                                                        >  1:8049/1‑150 (MQ=255)
   cagcaTGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTc                                                                                                                                                    >  2:196684/1‑150 (MQ=255)
    agcaTGCGCGCCTATGTGCAGGCCGTATAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCg                                                                                                                                                   <  1:479373/150‑1 (MQ=255)
     gcaTGCGAGCCTGTGTGCAGGCCGCCCGCGGTAATGATAGCTGCCGTGGTGGCTACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGAC‑TGGCAGCAGCCATGTCgg                                                                                                                                                  <  2:1646231/150‑1 (MQ=255)
      caTGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCggg                                                                                                                                                 <  1:1883002/150‑1 (MQ=255)
      caTGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGAC‑TGGCAGCAGCCATGTCggg                                                                                                                                                 <  1:1118570/150‑1 (MQ=255)
       aTGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCgggg                                                                                                                                                <  1:1823272/150‑1 (MQ=255)
        tGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGt                                                                                                                                               <  1:1505217/150‑1 (MQ=255)
             gcCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGtttttt                                                                                                                                          <  2:280502/150‑1 (MQ=255)
               cTATGTGCAGGCTGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCa                                                                                                                                        <  2:985638/150‑1 (MQ=255)
               cTATGTGCAGGCCGTGTAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCa                                                                                                                                        >  2:1279882/1‑150 (MQ=255)
               cTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCa                                                                                                                                        >  1:1428272/1‑150 (MQ=255)
               cTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCa                                                                                                                                        >  2:1761065/1‑150 (MQ=255)
                    tgCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTc                                                                                                                                         <  2:167462/144‑1 (MQ=255)
                    tgCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTc                                                                                                                                         >  1:167462/1‑144 (MQ=255)
                      cAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCa                                                                                                                                 <  2:473356/150‑1 (MQ=255)
                       aGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAg                                                                                                                                <  2:1428272/150‑1 (MQ=255)
                       aGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAg                                                                                                                                <  1:1026386/150‑1 (MQ=255)
                            gTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATGATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAg                                                                                                                           <  2:208683/150‑1 (MQ=255)
                                aGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATAATGAATGTCACCGGGACCAGTCGCGTTTCGCACTGTCCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAg                                                                                                                       <  1:1376332/150‑1 (MQ=255)
                                 ggAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCggg                                                                                                                                                 <  1:598110/123‑1 (MQ=255)
                                 ggAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCggg                                                                                                                                                 >  2:598110/1‑123 (MQ=255)
                                  gagaACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGc                                                                                                                     >  2:664889/1‑150 (MQ=255)
                                      aCGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATg                                                                                                                 <  1:664889/150‑1 (MQ=255)
                                      aCGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATg                                                                                                                 <  1:1200812/150‑1 (MQ=255)
                                           aTCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGt                                                                                                            <  2:102095/150‑1 (MQ=255)
                                              ttCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGtttt                                                                                                         >  1:733307/1‑150 (MQ=255)
                                              ttCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGtttt                                                                                                         >  1:282795/1‑150 (MQ=255)
                                                       ggCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGc                                                                                                >  1:624162/1‑150 (MQ=255)
                                                       ggCGACCGAGTACGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGc                                                                                                >  2:551259/1‑150 (MQ=255)
                                                          gACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCAt                                                                                             >  1:1253573/1‑150 (MQ=255)
                                                              gAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGCGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCg                                                                                         <  1:1023789/150‑1 (MQ=255)
                                                                gTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGt                                                                                       <  1:765825/150‑1 (MQ=255)
                                                                   cgcATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGAc                                                                                    <  1:282154/150‑1 (MQ=255)
                                                                     catcatGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACgg                                                                                  >  2:638614/1‑150 (MQ=255)
                                                                     catcatGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GGCGGGGTTTTTTCAGGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACgg                                                                                  >  2:1035335/1‑150 (MQ=255)
                                                                      atcatGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGc                                                                                 <  1:1140161/150‑1 (MQ=255)
                                                                        catGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGt                                                                               <  2:1238678/150‑1 (MQ=255)
                                                                          tGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGt                                                                                                                                               >  1:1101595/1‑84 (MQ=255)
                                                                          tGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGt                                                                                                                                               <  2:1101595/84‑1 (MQ=255)
                                                                          tGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAg                                                                             >  1:1033966/1‑150 (MQ=255)
                                                                             cTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGTGGTGTGCGGTTACGGTGACGGCGGAGCCg                                                                          >  2:904195/1‑150 (MQ=255)
                                                                              tGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGa                                                                         >  1:527007/1‑150 (MQ=255)
                                                                              tGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGa                                                                         <  2:8049/150‑1 (MQ=255)
                                                                                tAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGa                                                                         <  1:1280894/148‑1 (MQ=255)
                                                                                tAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTATTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGa                                                                         >  2:1280894/1‑148 (MQ=255)
                                                                                            gTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAg                                                           >  2:1231355/1‑150 (MQ=255)
                                                                                            gTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAg                                                           >  2:1503883/1‑150 (MQ=255)
                                                                                              cgcgTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAgaa                                                         <  1:904195/150‑1 (MQ=255)
                                                                                                  tttCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTg                                                                                                  >  1:1111098/1‑105 (MQ=255)
                                                                                                  tttCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTg                                                                                                  <  2:1111098/105‑1 (MQ=255)
                                                                                                  tttCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAgaangaa                                                     <  2:1202404/150‑1 (MQ=255)
                                                                                                     cGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCa                                                  <  2:1594623/150‑1 (MQ=255)
                                                                                                         cTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGCACGAACCAGTaa                                              >  1:374883/1‑150 (MQ=255)
                                                                                                                    gCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATg                                   <  1:551259/150‑1 (MQ=255)
                                                                                                                       gcatgaaaaaaCCCCGA‑GTGGCAGCAGCCATGGCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGCTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCgg                                >  2:118635/6‑150 (MQ=255)
                                                                                                                        taataaAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGt                               <  1:196684/150‑1 (MQ=255)
                                                                                                                        taataaAAAACCCCGACATGGCTGCTGCCA‑GTAGGGGTCTTTTCCTCCATCACCCAAGGCAGACAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGt                               >  1:515342/1‑150 (MQ=255)
                                                                                                                         aataaAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCAt                                    <  2:1315616/144‑1 (MQ=255)
                                                                                                                         aataaAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCAt                                    >  1:1315616/1‑144 (MQ=255)
                                                                                                                         aataaAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTc                              >  1:48153/1‑150 (MQ=255)
                                                                                                                                 aCCCCGAC‑TGGCAGCAGCCATGTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGTCGGCGTAGCCGATCACCGCAGCAAAGAACGAACCGGTAAGAATGCCCATGCGGTCCCTGCCgg                      >  2:1613271/1‑150 (MQ=255)
                                                                                                                                  ccccGAC‑TGGCAGCAGCCATGTCGGGGTTTTTTCATTCTTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCGGc                     >  2:1520529/1‑150 (MQ=255)
                                                                                                                                   cccGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACg                                                                                   <  1:1831859/87‑1 (MQ=255)
                                                                                                                                   cccGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACg                                                                                   >  2:1831859/1‑87 (MQ=255)
                                                                                                                                   cccGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCGGCg                    >  1:872152/1‑150 (MQ=255)
                                                                                                                                   cccGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCGGCg                    >  1:1258979/1‑150 (MQ=255)
                                                                                                                                   cccGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCGGCg                    >  2:1409489/1‑150 (MQ=255)
                                                                                                                                   cccGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGGAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCGGCg                    >  2:1409146/1‑150 (MQ=255)
                                                                                                                                    ccGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGGAAGAATGCCCATGCGGTCCATGCCGGCgg                   >  1:1713726/1‑149 (MQ=255)
                                                                                                                                     cGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCGGCGTa                  >  1:443832/1‑150 (MQ=255)
                                                                                                                                      gACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCGGCGTAc                 <  2:48153/150‑1 (MQ=255)
                                                                                                                                            gctgctGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGAt                                                                        <  1:52337/89‑1 (MQ=255)
                                                                                                                                            gctgctGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGAt                                                                        >  2:52337/1‑89 (MQ=255)
                                                                                                                                              tgctgcCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGGCGGCGGAGGCGCTTGCCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCGGCGTACTCACTGcc         >  2:689252/1‑149 (MQ=255)
                                                                                                                                              tgctgcCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCGGCGTACTCACTGcc         >  2:1393768/1‑149 (MQ=255)
                                                                                                                                                  gcCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCGGCGTACTCACTGCTAccc     <  2:624162/150‑1 (MQ=255)
                                                                                                                                                   ccA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAgaac                                                        >  2:454156/1‑98 (MQ=255)
                                                                                                                                                   ccA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAgaac                                                        <  1:454156/98‑1 (MQ=255)
                                                                                                                                                     a‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGGCGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCGGCGTACTCACTGCTACCCggg  <  2:1253573/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
TGGCAGCATGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCGGCGTACTCACTGCTACCCGGG  >  NC_002947/1294882‑1295180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑