BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,408,096:1	+T	5.1%	coding (491/615 nt)	PP_3021 ←	LysE family transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,408,096	1	.	T	5.1%	412.1 / 13.8	99	coding (491/615 nt)	PP_3021	LysE family transporter
Reads supporting (aligned to +/- strand): ref base . (46/48); new base T (2/3); total (48/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.23e-01

Only 100 of 101 total aligned reads displayed.

GACATGCGCACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTGAGGATGCCAGACAAGAAGCC  >  NC_002947/3407950‑3408244

gACATGCGCACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACGACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑Cgg                                                                                                                                                   <  1:770015/150‑1 (MQ=255)

   aTGCGCACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGa                                                                                                                                                <  2:423888/150‑1 (MQ=255)

    tGCGCACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGAc                                                                                                                                               <  2:23283/150‑1 (MQ=255)

      cgcACCTTGCATCAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCTGCTGGTCGCCG‑TGGCCATCGGCAACCa                                                                                                                                             <  1:1225726/150‑1 (MQ=255)

         aCCTTGCAACAAGTCAGCCCCGGGCGAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCa                                                                                                                                          >  1:706794/1‑150 (MQ=255)

          ccTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCaa                                                                                                                                         <  1:288167/150‑1 (MQ=255)

          ccTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCACGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCaa                                                                                                                                         <  2:1281302/150‑1 (MQ=255)

               caacaaGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTccc                                                                                                                                    >  2:33192/1‑150 (MQ=255)

                aacaaGTCAGCCCCGGGCGAGATGCAGCAACAGGGCCCTCACCAACAGGACCGGGATTACCCCCGGAAGGGGCGCCAGCCATGCCTTGTTGCTGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCC‑CCGGAGCAAGCAAGTCCCa                                                                                                                                   >  1:1077638/1‑150 (MQ=255)

                aacaaGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCGCa                                                                                                                                   >  2:828622/1‑150 (MQ=255)

                   aaGCCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGACAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCagag                                                                                                                                <  1:271249/150‑1 (MQ=255)

                    aGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGc                                                                                                                               >  1:10284/1‑150 (MQ=255)

                     gTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCa                                                                                                                              >  1:221330/1‑150 (MQ=255)

                        aGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑c                                                                                                                                                     >  2:1721786/1‑124 (MQ=255)

                        aGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑c                                                                                                                                                     <  1:1721786/124‑1 (MQ=255)

                        aGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGa                                                                                                                           >  2:1497388/1‑150 (MQ=255)

                            ccGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGAt                                                                                                                       <  1:1677747/150‑1 (MQ=255)

                            ccGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGAt                                                                                                                       >  2:481314/1‑150 (MQ=255)

                             cGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATg                                                                                                                      >  2:666142/1‑150 (MQ=255)

                             cGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATg                                                                                                                      >  1:1168706/1‑150 (MQ=255)

                                  cAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGAAGATGCTGaa                                                                                                                 >  1:1022051/1‑150 (MQ=255)

                                   aGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCCGAGCAGGACGATGCTGAAc                                                                                                                >  2:1759240/1‑150 (MQ=255)

                                     aTGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACAt                                                                                                              >  1:1062826/1‑150 (MQ=255)

                                            aacaacGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAgc                                                                                                       <  2:1171913/150‑1 (MQ=255)

                                             acaacGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAgcg                                                                                                      >  1:1099664/1‑150 (MQ=255)

                                             acaacGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAgcg                                                                                                      >  1:1064933/1‑150 (MQ=255)

                                               accGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGcgcg                                                                                                    <  1:173179/148‑1 (MQ=255)

                                                acGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGt                                                                                                   <  2:656381/150‑1 (MQ=255)

                                                    ccccGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGc                                                                                               >  2:686745/1‑150 (MQ=255)

                                                      ccGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCg                                                                                             >  1:1517916/1‑150 (MQ=255)

                                                        gCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGtt                                                                                           <  1:864792/150‑1 (MQ=255)

                                                            aCATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTcc                                                                                       >  1:355803/1‑150 (MQ=255)

                                                            aCATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTcc                                                                                       >  1:1464812/1‑150 (MQ=255)

                                                              aTCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGGAGCTCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAg                                                                                     >  1:1572978/1‑150 (MQ=255)

                                                              aTCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCTCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGttt                                                                                          >  2:519663/1‑145 (MQ=255)

                                                              aTCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCTCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGttt                                                                                          <  1:519663/145‑1 (MQ=255)

                                                              aTCACCAGCATGACCCCCGAGGCACGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAg                                                                                     >  1:573118/1‑150 (MQ=255)

                                                                 aCCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCg                                                                                  >  1:1030551/1‑150 (MQ=255)

                                                                  ccAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCgg                                                                                 <  1:828622/150‑1 (MQ=255)

                                                                   cAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTc                                                                                        <  1:1226739/142‑1 (MQ=255)

                                                                   cAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTc                                                                                        >  2:1226739/1‑142 (MQ=255)

                                                                   cAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCggg                                                                                <  2:1062826/150‑1 (MQ=255)

                                                                   cAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCggg                                                                                >  2:1643543/1‑150 (MQ=255)

                                                                   cAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCggg                                                                                >  1:285802/1‑150 (MQ=255)

                                                                   cAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCggg                                                                                >  1:283841/1‑150 (MQ=255)

                                                                   cAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCggg                                                                                <  2:446001/150‑1 (MQ=255)

                                                                    aGCATGACCCCCGCGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGGTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCgggg                                                                               <  2:655059/150‑1 (MQ=255)

                                                                    aGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCgggg                                                                               <  1:666142/150‑1 (MQ=255)

                                                                        tGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGa                                                                           <  2:925956/150‑1 (MQ=255)

                                                                         gACCCCCGCGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGAc                                                                          >  2:1662436/1‑150 (MQ=255)

                                                                           cccccGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGc                                                                        <  1:686745/150‑1 (MQ=255)

                                                                           cccccGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGc                                                                        >  2:124248/1‑150 (MQ=255)

                                                                            ccccGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCt                                                                       >  1:1886743/1‑150 (MQ=255)

                                                                              ccGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTgg                                                                     >  2:1782216/1‑150 (MQ=255)

                                                                                 aGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGa                                                                  <  2:1168706/150‑1 (MQ=255)

                                                                                    cccGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCTGCTGGTCGCCG‑TGGCCATCGGCAACCAGcgg                                                                                                                                         <  1:1720501/76‑3 (MQ=18)

                                                                                    cccGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCTGCTGGTCGCCG‑TGGCCATCGGCAACCAGcgg                                                                                                                                         >  2:1720501/1‑74 (MQ=18)

                                                                                     ccGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCaa                                                                                                                                         >  1:575883/1‑75 (MQ=255)

                                                                                     ccGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCaa                                                                                                                                         <  2:575883/75‑1 (MQ=255)

                                                                                      cGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATg                                                             <  2:1749101/150‑1 (MQ=255)

                                                                                      cGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATg                                                             >  2:1431183/1‑150 (MQ=255)

                                                                                          ccAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTgg                                                         <  1:1782216/150‑1 (MQ=255)

                                                                                            aGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGcc                                                       >  2:1440020/1‑150 (MQ=255)

                                                                                             gCCACACCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTcc                                                                                                                                     <  2:595439/71‑1 (MQ=255)

                                                                                             gCCACACCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTcc                                                                                                                                     >  1:595439/1‑71 (MQ=255)

                                                                                               cACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCt                                                                                                                    <  1:533946/86‑1 (MQ=255)

                                                                                               cACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCt                                                                                                                    >  2:533946/1‑86 (MQ=255)

                                                                                               cACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCggg                                                    >  1:200745/1‑150 (MQ=255)

                                                                                                aCGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGc                                                   <  2:285802/150‑1 (MQ=255)

                                                                                                aCGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGc                                                   <  2:283841/150‑1 (MQ=255)

                                                                                                    cAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGc                                               <  2:355803/150‑1 (MQ=255)

                                                                                                     aGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCa                                              <  1:1497388/150‑1 (MQ=255)

                                                                                                       gCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATa                                            <  2:221330/150‑1 (MQ=255)

                                                                                                         tGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTgacttgtt                                          <  2:1077638/150‑8 (MQ=255)

                                                                                                         tGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGa                                          >  2:645493/1‑150 (MQ=255)

                                                                                                                    cgccgcAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTc                               <  1:124248/150‑1 (MQ=255)

                                                                                                                       cgcAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTg                            <  2:1548837/150‑1 (MQ=255)

                                                                                                                       cgcAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCACGGTCTTg                            >  2:583524/1‑150 (MQ=255)

                                                                                                                        gcAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTgg                           <  2:10284/150‑1 (MQ=255)

                                                                                                                        gcAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTgg                           <  2:1517916/150‑1 (MQ=255)

                                                                                                                        gcAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTgg                           >  2:516511/1‑150 (MQ=255)

                                                                                                                         cAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTggg                          <  1:481314/150‑1 (MQ=255)

                                                                                                                           gTACCCGCGGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGATGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTTGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGt                        <  1:1525056/150‑1 (MQ=255)

                                                                                                                            tACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGtt                       <  1:1733001/150‑1 (MQ=255)

                                                                                                                              cccGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTGa                     <  2:573118/150‑1 (MQ=255)

                                                                                                                               ttgCTGGTCGCCG‑TGGCCATCGGCAACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGaa                                         >  2:1784327/3‑129 (MQ=38)

                                                                                                                               ttgCTGGTCGCCG‑TGGCCATCGGCAACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGaa                                         <  1:1784327/127‑1 (MQ=38)

                                                                                                                               ccGCTGGTTGCCGCTGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGAAGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTGAg                    <  2:1350182/150‑1 (MQ=255)

                                                                                                                               ccGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCa                                                               >  2:1556307/1‑107 (MQ=255)

                                                                                                                               ccGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCa                                                               <  1:1556307/107‑1 (MQ=255)

                                                                                                                               ccGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTGAg                    <  2:1218911/150‑1 (MQ=255)

                                                                                                                               ccGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTGAg                    >  1:91972/1‑150 (MQ=255)

                                                                                                                                    ggTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTGAGGATGc               <  1:33192/150‑1 (MQ=255)

                                                                                                                                      ttGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTGAGGATGCCa             <  1:1643543/150‑1 (MQ=255)

                                                                                                                                        gCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTGAGGATGCCAGa           <  2:1572978/150‑1 (MQ=255)

                                                                                                                                         ccGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGtt                                                                                           <  2:577010/69‑1 (MQ=255)

                                                                                                                                         ccGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGtt                                                                                           >  1:577010/1‑69 (MQ=255)

                                                                                                                                          cGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTGAGGATGCCAGACa         >  1:875585/1‑150 (MQ=255)

                                                                                                                                                ccA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCCTCCACTTCCACGTTCTTGGGGTTGAGGATGCCAGACAAGAAGc   >  2:1894201/1‑150 (MQ=255)

                                                                                                                                                 cA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTGAGGATGCCAGACAAGAAGcc  <  2:1030551/150‑1 (MQ=255)

GACATGCGCACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGATGGCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTGAGGATGCCAGACAAGAAGCC  >  NC_002947/3407950‑3408244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`