Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,460,273:1 +A 5.8% coding (132/1446 nt) PP_3075 ← transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,460,2731.A 5.8% 269.5 / 10.3 69coding (132/1446 nt)PP_3075transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (28/37);  new base A (2/2);  total (30/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.28e-01

AGGCTGCCGGGGATCACTGTTTCGCACGGCTGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAAGGCCAGGCCTGAAAACTCATCGTCGGGGGTCAAACGC  >  NC_002947/3460124‑3460421
                                                                                                                                                      |                                                                                                                                                    
aGGCTGCCGGGGATCACTGTTTCGCACGGCTGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGAtc                                                                                                                                                       <  1:762314/150‑1 (MQ=255)
     gCCGGGGATCACTGTTTCGCACGGCTGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGaa                                                                                                                                                 <  1:1414685/150‑1 (MQ=255)
       cGGGGATCACTGTTTCGCACGGCTGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAAct                                                                                                                                               <  1:1006410/150‑2 (MQ=255)
         gggATCACTGTTTCGCACGGCTGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACagc                                                                                                                                             >  1:1363490/1‑150 (MQ=255)
            aTCACTGTTTCGCACGGCTGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACagcgag                                                                                                                                          >  1:30808/1‑150 (MQ=255)
            aTCACTGTTTCGCACGGCTGGCCGATGGAACTCGTCGAGTCGGCCAGGCCTATTCTTCGGTCGTATCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACAGTGCCCTCGCTGGACTGTTCGATGATC‑TCTAACagcgag                                                                                                                                          <  2:880485/150‑1 (MQ=255)
                 tGTTTCGCACGGCTGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGAt                                                                                                                                     >  2:118489/1‑150 (MQ=255)
                 tGTTTCGCACGGCTGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGAt                                                                                                                                     <  1:856184/150‑1 (MQ=255)
                 tGTTTCGCACGGCTGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGA‑GATCATCGAACagt                                                                                                                                             <  1:379106/142‑2 (MQ=255)
                 tGTTTCGCACGGCTGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGA‑GATCATCGAACagt                                                                                                                                             >  2:379106/1‑141 (MQ=255)
                   tttCGCACGGCTGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACagcgagc                                                                                                                                         <  1:1613050/144‑1 (MQ=255)
                   tttCGCACGGCTGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACagcgagc                                                                                                                                         >  2:1613050/1‑144 (MQ=255)
                          cGGCTGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGACCACTTGTTCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTg                                                                                                                            <  2:464297/150‑1 (MQ=255)
                            gCTGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGcc                                                                                                                          >  2:768189/1‑150 (MQ=255)
                            gCTGGCAGATGGCACTGGCAGCGTCGGCCAGGCGAAAGGGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGGTCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGcc                                                                                                                          <  2:723820/150‑1 (MQ=255)
                              tGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGCCGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGt                                                                                                                        >  2:1410677/1‑150 (MQ=255)
                                gCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTAc                                                                                                                      >  2:1745961/1‑150 (MQ=255)
                                 ccGATGGCACTGGCAGCGTCGGCCAGGCAAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGTGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTAcc                                                                                                                     <  2:243208/150‑1 (MQ=255)
                                    aTGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTgg                                                                                                                  <  1:177450/150‑1 (MQ=255)
                                    aTGGCACTGGCAGCGTCGGCCAGGCACAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTgg                                                                                                                  <  2:885989/150‑1 (MQ=255)
                                     tGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTggg                                                                                                                 <  2:1363490/150‑1 (MQ=255)
                                      ggCGCTGGCAGCGTGGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTGGACGATCACAGTGCCCTCGCTGGCCCGTTGGTGGATG‑TCGAACAGCGAGCGGATCGCCAGCTGCCGTACCTgggg                                                                                                                <  2:1213224/150‑1 (MQ=255)
                                       gCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTCGCGTTCCTTCATCCAGCCGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTgnggg                                                                                                               <  2:1196850/150‑1 (MQ=255)
                                            ggCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACagc                                                                                                                                             >  1:1092360/1‑115 (MQ=255)
                                            ggCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACagc                                                                                                                                             <  2:1092360/115‑1 (MQ=255)
                                             gCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTgggg                                                                                                                >  2:1208791/1‑143 (MQ=255)
                                             gCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTgggg                                                                                                                <  1:1208791/143‑1 (MQ=255)
                                                   tCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAgg                                                                                                   <  2:1593993/150‑1 (MQ=255)
                                                    cGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGGTC‑TCGAACATCAAGCTGAGCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGgc                                                                                                  <  1:37291/150‑1 (MQ=255)
                                                      gccaggccaAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGgctg                                                                                                >  1:1422511/1‑150 (MQ=255)
                                                       ccaggccaAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGgctgg                                                                                               >  2:1535581/1‑149 (MQ=255)
                                                       ccaggccaAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATAACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGgctgt                                                                                               <  1:118489/150‑1 (MQ=255)
                                                          ggccaAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGA‑GATCATCGAACa                                                                                                                                               <  1:860038/99‑1 (MQ=255)
                                                          ggccaAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGA‑GATCATCGAACa                                                                                                                                               >  2:860038/1‑99 (MQ=255)
                                                             caAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGgctgtcgctgt                                                                                         >  1:853644/1‑150 (MQ=255)
                                                               aaGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGctgtcgctgtcg                                                                                       >  2:1747264/1‑150 (MQ=255)
                                                                      gggCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATgg                                                                                >  1:1567193/1‑150 (MQ=255)
                                                                         cGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTg                                                                             <  1:1031025/150‑1 (MQ=255)
                                                                                ttcgttcATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTa                                                                                                             >  2:1207969/1‑111 (MQ=255)
                                                                                ttcgttcATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTa                                                                                                             <  1:1207969/111‑1 (MQ=255)
                                                                                   gttcATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGa                                                                   >  2:917129/1‑150 (MQ=255)
                                                                                    ttcATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGaa                                                                  >  2:1416904/1‑150 (MQ=255)
                                                                                        tCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCAt                                                              <  1:1105940/150‑1 (MQ=255)
                                                                                        cccacctgATCCGTTCCTGGCGGTCGACGATCACCGTGCCCCCGATGTGGTGTGTGATGATC‑TCGAACAGCATTTTCTGCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCAt                                                              <  1:889631/143‑1 (MQ=255)
                                                                                            gACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTAt                                                          >  2:847462/1‑150 (MQ=255)
                                                                                              cGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTg                                                        <  2:1230718/150‑1 (MQ=255)
                                                                                              cGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTg                                                        <  2:1857405/150‑1 (MQ=255)
                                                                                                 tGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACagcgag                                                                                                                                          >  1:904641/1‑65 (MQ=255)
                                                                                                 tGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACagcgag                                                                                                                                          <  2:904641/65‑1 (MQ=255)
                                                                                                  gCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGcac                                                    <  2:1414507/150‑1 (MQ=255)
                                                                                                    gTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGaa                                                                  >  2:1173758/1‑134 (MQ=255)
                                                                                                    gTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGaa                                                                  <  1:1173758/134‑1 (MQ=255)
                                                                                                        cTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGccc                                              <  1:39620/150‑1 (MQ=255)
                                                                                                          ggCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCcgc                                            <  1:220922/150‑1 (MQ=255)
                                                                                                               tCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGaaaa                                       <  2:1422511/150‑1 (MQ=255)
                                                                                                               tCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGaaaa                                       <  2:656494/150‑1 (MQ=255)
                                                                                                                cgacgaTCGCCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAag                                      <  2:144380/150‑1 (MQ=255)
                                                                                                                 gacgaTCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAagg                                     >  1:87700/1‑150 (MQ=255)
                                                                                                                 gacgaTCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGAGAGAGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAagg                                     >  2:265874/1‑150 (MQ=255)
                                                                                                                   cgaTCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAaggcc                                   >  1:8914/1‑150 (MQ=255)
                                                                                                                    gaTCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGACGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAaggcca                                  >  2:1435626/1‑150 (MQ=255)
                                                                                                                      tCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAaggccagg                                <  1:1747264/150‑1 (MQ=255)
                                                                                                                           gTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAAGGCCAGGCCTGa                           <  2:1328155/150‑1 (MQ=255)
                                                                                                                                cTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAAGGCCAGGCCTGAAAACt                      >  2:1471402/1‑150 (MQ=255)
                                                                                                                                 tCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAAGGCCAGGCCTGAAAACTc                     <  2:1567193/150‑1 (MQ=255)
                                                                                                                                    cTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAAGGCCAGGCCTGAAAACTCAtc                  >  2:244858/1‑150 (MQ=255)
                                                                                                                                     tGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGaaaa                                       >  2:1865116/1‑128 (MQ=255)
                                                                                                                                     tGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGaaaa                                       <  1:1865116/128‑1 (MQ=255)
                                                                                                                                               gatgatC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAAGGCCAGGCCTGAAAACTCATCGTCGGGGGTCa       >  1:1029108/1‑150 (MQ=255)
                                                                                                                                                 tgatC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAAGGCCAGGCCTGAAAACTCATCGTCGGGGGTCaaa     <  1:847462/150‑1 (MQ=255)
                                                                                                                                                    tc‑tcGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAAGGCCAGGCCTGAAAACTCATCGTCGGGGGTCAAACGc  >  2:530333/1‑150 (MQ=255)
                                                                                                                                                    tc‑tcGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTAACTGACGTGCAAAGGAAATCAGTCTGATTCATGTATTGGCACAAGCCCGCGAAAAGGCCAGGCCTGAAAACTCATCGTCGGGGGTCAAACGc  >  1:704823/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
AGGCTGCCGGGGATCACTGTTTCGCACGGCTGGCCGATGGCACTGGCAGCGTCGGCCAGGCCAAAGCGTCGGGCGTAGCGTTCGTTCATCCAGACGATGCGTGCCTGGCGGTCGACGATCACCGTGCCCTCGCTGGACTGTTCGATGATC‑TCGAACAGCGAGCGGATCGCCAGTTGCCGTACCTGGGGGTAGTCCTTGAGGCTGTCGCTGTCGTGCATGGGTGAACATCCTGAATCATGTATTGGCACAAGCCCGCGAAAAGGCCAGGCCTGAAAACTCATCGTCGGGGGTCAAACGC  >  NC_002947/3460124‑3460421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑