| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 305,156:1 | +A | 6.9% | intergenic (+13/+16) | hslR → / ← PP_0251 | heat shock protein Hsp15/membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 305,156 | 1 | . | A | 6.9% | 223.0 / 10.6 | 58 | intergenic (+13/+16) | hslR/PP_0251 | heat shock protein Hsp15/membrane protein |
| Reads supporting (aligned to +/- strand): ref base . (30/24); new base A (2/2); total (32/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.63e-01 | |||||||||||
CGAAGAAAGTGTGCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCTCCAGCCCAGCGC > NC_002947/305011‑305300 | cGAAGAAAGTGTGCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAg > 2:420128/1‑150 (MQ=255) gaagaaAGTGTGCGTCGCCGTGAACCGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACTATTGAATGGGGCTGCTGC‑GCAGc < 1:1574614/150‑1 (MQ=255) agaaAGTGTGCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGccc < 2:920274/150‑1 (MQ=255) aGTGTGCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGC‑GCAGCAGCCCCatt < 1:760138/150‑3 (MQ=255) tCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAg < 1:420128/150‑1 (MQ=255) ccGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCtgctgc < 1:375597/128‑1 (MQ=255) ccGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCtgctgc > 2:375597/1‑128 (MQ=255) ccGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAgcgc > 2:246704/1‑150 (MQ=255) gTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGC‑GCAGCAGCCCCat > 1:1298912/1‑135 (MQ=255) gTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGC‑GCAGCAGCCCCat < 2:1298912/136‑2 (MQ=255) aaCAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAacac > 1:1412009/1‑150 (MQ=255) gcggcTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGCTTTCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCa > 1:1535639/1‑150 (MQ=255) ggcTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCAtt > 2:1814798/1‑150 (MQ=255) gcTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGcc < 2:728352/121‑1 (MQ=255) gcTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGcc > 1:728352/1‑121 (MQ=255) gcTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTc > 1:29925/1‑150 (MQ=255) aaaTGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTcc < 2:1898738/150‑1 (MQ=255) aaTGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGTGGCTGCTGC‑GTAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCg > 1:1187691/1‑150 (MQ=255) cGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAAc > 2:668194/1‑150 (MQ=255) ggCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTg < 1:246704/150‑1 (MQ=255) tGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCtt < 2:29925/150‑1 (MQ=255) tGACCACAGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGCTTAAACGCTTGTAAGCGCCTAACAGCACAGTGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCtt < 1:1663475/150‑1 (MQ=255) ccaccGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGaa < 1:668194/150‑1 (MQ=255) accGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGaaca > 1:207616/1‑150 (MQ=255) gccggccgACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGgcgc > 1:128592/1‑150 (MQ=255) cggccgACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAgcg > 2:1492596/1‑91 (MQ=255) cggccgACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAgcg < 1:1492596/91‑1 (MQ=255) aCCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAgc < 1:62031/150‑1 (MQ=255) cAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCt > 2:1394606/1‑127 (MQ=255) cAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCt < 1:1394606/127‑1 (MQ=255) cAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAgcgc > 1:718150/1‑150 (MQ=255) agaagCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCAt < 2:1412009/150‑1 (MQ=255) gcagcGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAgc < 1:698969/76‑1 (MQ=255) gcagcGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAgc > 2:698969/1‑76 (MQ=255) gcagcGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAAc < 2:1719801/150‑1 (MQ=255) gcagcGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAAc > 1:1719801/1‑150 (MQ=255) gcagcGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAAc < 1:619215/150‑1 (MQ=255) cagcGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACg > 2:809135/1‑129 (MQ=255) cagcGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACg < 1:809135/129‑1 (MQ=255) gcAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTg > 2:412815/1‑150 (MQ=255) gcAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTg > 1:1324079/1‑150 (MQ=255) gcAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGAGATGGCAGAGGCCAAGGGTAGCGTGGGGAGCAGGTTGAACACAGGCGCTGTTCAGCGCATCAACGCCTCGCTg > 1:974599/1‑150 (MQ=255) gATTCACCAGCTGCATGGAAGTTACGATTGAATGTGGCTGCTGC‑GTAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTNCCt < 2:1187691/150‑1 (MQ=255) aTTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCtt > 1:446435/1‑150 (MQ=255) ttCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCACCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCttt < 1:1690118/150‑1 (MQ=255) tCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTg < 2:1324079/150‑1 (MQ=255) cACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTAC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTgg < 2:1465444/150‑1 (MQ=255) aCCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGgc > 2:1385016/1‑150 (MQ=255) aGCTGCATGGAAGTTACGATTGAATGGCAATGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGgccgc > 1:51092/1‑150 (MQ=255) cATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAgcg > 2:787871/1‑150 (MQ=255) aTGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGgccgtactggc > 2:779673/1‑143 (MQ=255) tGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAATAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAgcggc > 2:1371955/1‑150 (MQ=255) gTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTgta < 1:46128/150‑1 (MQ=255) cGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGtagtag < 2:379096/150‑1 (MQ=255) gATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGc > 2:621979/1‑150 (MQ=255) gATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATATAGCGCAACACCGACATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGc < 1:1858559/150‑1 (MQ=255) attttttGGGGCTGC‑GCAGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCt > 2:777482/7‑150 (MQ=255) aTTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCt > 1:220358/1‑150 (MQ=255) ttGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTGTGGCCGCCAGCGGCGTGTAGTAGCTc > 2:1680034/1‑150 (MQ=255) aaTGGGGCTGCTGC‑GCAGCCCCAAAAAAGCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGAGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCTccag > 2:998702/1‑150 (MQ=255) tgctgc‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCTCCAGCCCAgcgc > 2:241252/1‑150 (MQ=255) | CGAAGAAAGTGTGCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGC‑GCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCTCCAGCCCAGCGC > NC_002947/305011‑305300 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |