Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,001,855 C→A 9.4% intergenic (+1277/‑409) PP_4407 → / → PP_4409 gamma‑aminobutyrate transporter/site‑specific recombinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,001,8550CA 9.4% 147.6 / 2.6 51intergenic (+1277/‑409)PP_4407/PP_4409gamma‑aminobutyrate transporter/site‑specific recombinase
Reads supporting (aligned to +/- strand):  ref base C (23/23);  new base A (2/3);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACCGCCGGATCGTAACGCGCTTCGACAAATTCGCGAAAAGCTACGCCGCCATGGTCTCACTGGCTTGTTCCATGCGGTGTTTGCGACATCTCCTTTCGTACGGAGCCTAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACCACAAAGCCCAAAATCCAAAGCCTGACAAACAAATATACGAACAATTGACAGTAATTAGAGACCCAATTAAAAACAACAGCTTACCAACAAAA  >  NC_002947/5001707‑5002004
                                                                                                                                                    |                                                                                                                                                     
aaCCGCCGGATCGTAACGCGCTTCGACAAATTCGCGAAAAGCTACGCCGCCATGGTCTCACTGGCTTGTTCCATGCGGTGTTTGCGACATCTCCTTTCGTACGGAGCCTAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACCg                                                                                                                                                      >  1:836676/1‑150 (MQ=255)
              aaCGCGCTTCGACAAATTCGCGAAAAGCTACGCCGCCATGGTCTCACTGGCTTGTTCCATGCGGTGTTTGCGACATCTCCTTTCGTACGGAGCCTAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAatat                                                                                                                                        >  1:980128/1‑150 (MQ=255)
                  cgcTTCGACAAATTCGCGAAAAGCTACGCCGCCATGGTCTCACTGGCTTGTTCCATGCGGTGTTTGCGACATCTCCTTTCGTACGGAGCCTAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTaa                                                                                                                                    <  1:964832/150‑1 (MQ=255)
                                      aaGCTACGCCGCCATGGTCTCACTGGCTTGTTCCATGCGGTGTTTGCGACATCTCCTTTCGTACGGAGCCTAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCAcc                                                                                                                <  2:1178648/150‑1 (MQ=255)
                                          tACGCCGCCATGGTCTCACTGGCTTGTTCCATGCGGTGTTTGCGACATCTCCTTTCGTACGGAGCCTAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCAc                                                                                                                 <  1:587403/145‑1 (MQ=255)
                                          tACGCCGCCATGGTCTCACTGGCTTGTTCCATGCGGTGTTTGCGACATCTCCTTTCGTACGGAGCCTAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCAc                                                                                                                 >  2:587403/1‑145 (MQ=255)
                                                    tGGTCTCACTGGCTTGTTCCATGCGGTGTTTGCGACATCTCCTTTCGTACGGAGCCTAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGAAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTgcg                                                                                                  <  1:741054/150‑1 (MQ=255)
                                                     ggTCTCACTGGCTTGTTCCATGCGGTGTTTGCGACATCTCCTTTCGTACGGAGCCTAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTgcgc                                                                                                 <  1:161540/150‑1 (MQ=255)
                                                       tctcACTGGCTTGTTCCATGCGGTGTTTGCGACATCTCCTTTCGTACGGAGCCTAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGcac                                                                                               >  2:1306295/1‑150 (MQ=255)
                                                          cACTGGCTTGTTCCATGCGGTGTTTGCGACATCTCCTTTCGTACGGAGCCTAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGcaccac                                                                                            >  1:1850850/1‑150 (MQ=255)
                                                          cACTGGCTTGTTCCATGCGGTGTTTGCGACATCTCCTTTCGTACGGAGCCTAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGcaccac                                                                                            >  1:1851104/1‑150 (MQ=255)
                                                           aCTGGCTTGTTCCATGCGGTGTTTGCGACATCTCCTTTCGTACGGATCATAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACATTACATTATTAATACACCATTAATAAAATGCTCACcaca                                                                                           >  2:436808/1‑150 (MQ=255)
                                                           aCTGGCTTGTTCCATGCGGTGTTTGCGACATCTCCTTTCGTACGGAGCCTAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACcaca                                                                                           >  1:1892652/1‑150 (MQ=255)
                                                                 ttGTTCCATGCGGTGTTTGCGACATCTCCTTTCGTACGGAGCCTAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACCACAAAGccc                                                                                     >  1:1252061/1‑150 (MQ=255)
                                                                        aTGCGGTGTTTGCGACATCTCCTTTCGTACGGAGCCTAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACCACAAAGCCCAAAATcc                                                                              >  1:537777/1‑150 (MQ=255)
                                                                            ggTGTTTGCGACATCTCCTTTCGTACGGAGCCTAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACCACAAAGCCCAAAATCCAAAg                                                                          <  1:779367/150‑1 (MQ=255)
                                                                                 ttGCGACATCTCCTTTCGTACGGAGCCTAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACCACAAAGCCCAAAATCCAAAGCCTGa                                                                     <  1:1306295/150‑1 (MQ=255)
                                                                                    cGACATCTCCTTTCGTACGGAGCCTAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACAGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACCACAAAGCCCAAAATCCAAAGCCTGacaa                                                                  >  2:722288/1‑150 (MQ=255)
                                                                                    cGACATCTCCTTTCGTACGGAGCCTAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACAGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACCACAAAGCCCAAAATCCAAAGCCTGacaa                                                                  >  2:722464/1‑150 (MQ=255)
                                                                                       cATCTCCTTTCGTACGGAGCCTAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACCACAAAGCCCAAAATCCAAAGCCTGacaaaca                                                               <  2:980128/150‑1 (MQ=255)
                                                                                         tctcCTTTCGTACGGAGCCTAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACCACAAAGCCCAAAATCCAAAGCCTGAcaaacaaa                                                             >  1:1855729/1‑150 (MQ=255)
                                                                                           ttcTTTCATACTGAGCCTAGCCAGCTCGACGACTGGGTTCTTTAGCCCGACAAGTACCGCTGGAAAGACATATTTAACAGTACATTATTAATGGGGCAGGAATAAAGTTGACAGCGCAACGCACGAAAACCAAAGCGTGACACACAAata                                                           >  2:212993/3‑150 (MQ=255)
                                                                                           tcCTTTCGTACGGAGCCTAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATACAGTGTGTATAACAAAGGCCAAAAACCAAAGCCGGACAAACAAata                                                           >  2:1646832/1‑150 (MQ=255)
                                                                                             cTTTCGTACGGAGCCTAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACCACAAAGCCCAAAATCCAAAGCCTGACAAACAAAtata                                                         >  1:489050/1‑150 (MQ=255)
                                                                                             cTTTCGTACGGAGCCTAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACCACAAAGCCCAAAATCCAAAGCCTGACAAACAAAtata                                                         <  2:1855729/150‑1 (MQ=255)
                                                                                                   tACGGAGCCTAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACCACAAAGCCCAAAATCCAAAGCCTGACAAACAAATATACGAACa                                                   >  2:1672809/1‑150 (MQ=255)
                                                                                                    aCGGAGCCTAGCCAGCCCGACTACTGGTTTCACGAGGCTGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTACTAAAGTGCGCACCACAAAGCCCAAAATCCAAAGCCTGACAAACAAATATACGAACaa                                                  <  2:614965/150‑1 (MQ=255)
                                                                                                         gCCTAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATaaa                                                                                                       >  1:1047265/1‑92 (MQ=255)
                                                                                                         gCCTAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATaaa                                                                                                       <  2:1047265/92‑1 (MQ=255)
                                                                                                          ccTAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACCACAAAGCCCAAAATCCAAAGCCTGACAAACAAATATACGAACAATTGACa                                            >  1:130433/1‑150 (MQ=255)
                                                                                                            tAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACCACAAAGCCCAAAATCCAAAGCCTGACAAACAAATATACGAACAATTGACAGt                                          >  1:1808043/1‑150 (MQ=255)
                                                                                                            tAGCCAGCCCGACTACTGGTTTCACGAAGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACGTTATTAATGCACCATTAATAAAGTGCGCACCACAAAGCCCAAAATCCAAAGCCTGACAAACAAATATACGAACAATTGACAGt                                          >  2:1657508/1‑150 (MQ=255)
                                                                                                                 agccCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACCACACAGCCCAAACTCCAAAGCCTGACAATCAAATATACGAACAATTGACAGTAATTa                                     <  1:467694/150‑1 (MQ=255)
                                                                                                                  gccCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACCACAAAGCCCAAAATCCAAAGCCTGACAAACAAATATAc                                                        <  2:467131/130‑1 (MQ=255)
                                                                                                                  gccCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACCACAAAGCCCAAAATCCAAAGCCTGACAAACAAATATAc                                                        >  1:467131/1‑130 (MQ=255)
                                                                                                                   cccGACTACTGGTTTCACGAGGCCGCCAAGTCCAGCTGCTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACCACAAAGCCCAAAATCCAAAGCCTGACAAACAAATATACGAACAATTGACAGTAATTaga                                   <  2:1892652/150‑1 (MQ=255)
                                                                                                                     cGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATCAATAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACCACAAAGCCCAAAATCCAAAGCCTGACAAACAAATATACGAACAATTGACAGTAATTAgaga                                 <  1:1646832/150‑1 (MQ=255)
                                                                                                                     cGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACCACAAAGCCCAAAATCCAAAGCCTGACAAACAAATATACGAACAATTGACAGTAATTAgaga                                 <  2:836676/150‑1 (MQ=255)
                                                                                                                        ctactGGTTTCACGCGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACCACAAAGCCCAAAATCCAAAGCCTGACAAACAAATATACGAACAATTGACAGTAATTAGAGAccc                              <  1:86800/150‑1 (MQ=255)
                                                                                                                         tactGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCTCCACAAAGcc                                                                                      >  1:1860640/1‑93 (MQ=255)
                                                                                                                         tactGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACCACAAAGcc                                                                                      <  2:1860640/93‑1 (MQ=255)
                                                                                                                           ctGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACCACAAAGCCCAAAATCCAAAGCCTGACAAACAAATATACGAACAATTGACAGTAATTAGAGACCCAAt                           >  2:1890234/1‑150 (MQ=255)
                                                                                                                              gTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTGATAAAGTGCGCACCACAAAGCCCAAAATCCAAAGCCTGACAAACAAATATACGAACAATTGACAGTAATTAGAGACCCAATTaa                        <  2:1492461/150‑1 (MQ=255)
                                                                                                                                ttCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACAATTAATAAAGTGCGCACCACAAAGCAAAAAATCCAAAGCCTGACAAACAAATATACGAACAATTGACAGTAATTAGAGACCCAATTaaaa                      <  2:390243/150‑1 (MQ=255)
                                                                                                                                     gAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGt                                                                                                     >  1:1070727/1‑66 (MQ=255)
                                                                                                                                     gAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGt                                                                                                     <  2:1070727/66‑1 (MQ=255)
                                                                                                                                          cgccAAGTACAGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACCACAAAGCCCAAAATCCAAAGCCTGACAAACAAATATACGAACAATTGACAGTAATTAGAGACCCAATTAAAAACAACAGCtt            <  1:722464/150‑1 (MQ=255)
                                                                                                                                          cgccAAGTACAGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACCACAAAGCCCAAAATCCAAAGCCTGACAAACAAATATACGAACAATTGACAGTAATTAGAGACCCAATTAAAAACAACAGCtt            <  1:722288/150‑1 (MQ=255)
                                                                                                                                             aaaGTAGCGCTGATCAGAAACATTTCACTGTACATTATGAATTCACTATTAATAAAGTGCGCACCAAAAAGCGGAAAATCCAAAGCATGACAAACAAATATACGAACAATTGACAGTAATTAGAGACCCAATTAAAAACAACAGCTTAcc         <  1:1278049/149‑1 (MQ=255)
                                                                                                                                               aGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACCACAAAGACCAAAATACAAAGCCTGAAAACCTAATATACGAACAATTGACAGTAATTAGAGACCCAATTAAAAACAACAGCTTACcaa       <  1:1672809/150‑1 (MQ=255)
                                                                                                                                                gTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACCACAAAGCCCAAAATCCAAAGCCTGACAAACAAATATACGAACAATTGACAGTAATTAGAGACCCAATTAAAAACAACAGCTTACcaac      <  2:1594690/150‑1 (MQ=255)
                                                                                                                                                    cGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACCACAAAGCCCAAAATCCAAAGCCTGACAAACAAATATACGAACAATTGACAGTCAGTAAATACCCAATTAAAAACAACAGCTTACCAACaaaa  >  2:448220/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AACCGCCGGATCGTAACGCGCTTCGACAAATTCGCGAAAAGCTACGCCGCCATGGTCTCACTGGCTTGTTCCATGCGGTGTTTGCGACATCTCCTTTCGTACGGAGCCTAGCCAGCCCGACTACTGGTTTCACGAGGCCGCCAAGTACCGCTGGTCGGAAATATTTAACAGTACATTATTAATGCACCATTAATAAAGTGCGCACCACAAAGCCCAAAATCCAAAGCCTGACAAACAAATATACGAACAATTGACAGTAATTAGAGACCCAATTAAAAACAACAGCTTACCAACAAAA  >  NC_002947/5001707‑5002004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑