Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,131,424 T→C 5.5% K10E (AAG→GAG)  recQ ← ATP‑dependent DNA 3'‑5' helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,131,4240TC 5.5% 251.8 / 2.3 84K10E (AAG→GAG) recQATP‑dependent DNA 3'‑5' helicase
Reads supporting (aligned to +/- strand):  ref base T (33/46);  new base C (3/2);  total (36/48)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAGCCCGGGCGCAGCAGCCCCGGCACCTGGAAACACAGCGATTTGCCGCCACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGAAAAACCCGCCCCGCACACGCCAAATGCGTAAGCGGTTTTTGCCAGGCAGCCATTGAAACCTGCCCCTGGA  >  NC_002947/5131275‑5131566
                                                                                                                                                      |                                                                                                                                                
cAAGCCCGGGCGCAGCAGCCCCGGCACCTGGAAACACAGCGATTTGCCGCCACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCtt                                                                                                                                                  >  2:1398909/1‑150 (MQ=255)
 aaGCCCGGGCGCAGCAGCCCCGGCACCTGGAAACACAGCGATTTGCCGCCACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACCGAAACTGTCGTAGCCGAAGATGTCCTTg                                                                                                                                                 <  1:849030/150‑1 (MQ=255)
  aGCCCGGGCGCAGCAGCCCCGGCACCTGGAAACACAGCGATTTGCCGCCACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTga                                                                                                                                                <  1:871611/150‑1 (MQ=255)
  aGCCCGGGCGCAGCAGCCCCGGCACCTGGAAACACAGCGATTTGCCGCCACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTga                                                                                                                                                <  1:273747/150‑1 (MQ=255)
       gggCGCAGCAGCCCCGGCACCTGGAAACACAGCGATTTGCCGCCACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAAcg                                                                                                                                           >  2:1212494/1‑150 (MQ=255)
          cgcAGCAGCCCCGGCACCTGGAAACACAGCGATTTGCCGCCACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACgcgc                                                                                                                                        <  1:488946/150‑1 (MQ=255)
            cagcagCCCCGGCACCTGGAAACACAGCGATTTGCCGCCACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTg                                                                                                                                      <  1:1864259/150‑1 (MQ=255)
               cagcCCCGGCACCTGGAAACACAGCGATTTGCCGCCACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGc                                                                                                                                   <  1:1847836/150‑1 (MQ=255)
                 gcCCCGGCACCTGGAAACACAGCGATTTGCCGCCACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCt                                                                                                                                 >  2:511601/1‑150 (MQ=255)
                    ccGGCACCTGGAAACACAGCGATTTGCCGCCACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGa                                                                                                                                     >  2:400364/1‑143 (MQ=255)
                    ccGGCACCTGGAAACACAGCGATTTGCCGCCACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGa                                                                                                                                     <  1:400364/143‑1 (MQ=255)
                    ccGGCACCTGGAAACACAGCGATTTGCCGCCACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGtt                                                                                                                              <  1:726535/150‑1 (MQ=255)
                    ccGGCACCTGGAAACACAGCGATTTGCCGCCACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAC‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGtt                                                                                                                              >  1:1531625/1‑150 (MQ=255)
                       gCACCTGGAAACACAGCGATTTGCCGCCACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGa                                                                                                                           <  1:1032655/150‑1 (MQ=255)
                       gCACCTGGAAACACAGCGATTTGCCGCCACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGa                                                                                                                           <  2:1237332/150‑1 (MQ=255)
                         aCCTGGAAACACAGCGATTTGCCGCCACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCNAG‑c                                                                                                                        <  2:1193893/150‑1 (MQ=255)
                         aCCTGGAAACACAGCGATTTGCCGCCACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑c                                                                                                                        <  2:615340/150‑1 (MQ=255)
                         aCCTGGAAACACAGCGATTTGCCGCCACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCAAGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑c                                                                                                                        <  2:524678/150‑1 (MQ=255)
                             ggAAACACAGCGATTTGCCGCCACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CAtgt                                                                                                                    <  1:377177/150‑1 (MQ=255)
                                aaCACAGCGATTTGCCGCCACCGCTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATCTGCa                                                                                                                 >  2:942514/1‑150 (MQ=255)
                                 acacAGCGATTTGCCGCCACCGGTGGGCATCAGCAGCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCaa                                                                                                                >  1:1399511/1‑150 (MQ=255)
                                   acaGCGATTTGCCGCCACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACt                                                                                                              >  2:197370/1‑150 (MQ=255)
                                   acaGCGATTTGCCGCCACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACt                                                                                                              >  2:1129338/1‑150 (MQ=255)
                                        gATTTGCCGCCACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCa‑aa                                                                                                        >  2:1610722/1‑150 (MQ=255)
                                          tttGCCGCCACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATc                                                                                                      >  2:1719845/1‑150 (MQ=255)
                                             gccgccACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGACCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGcc                                                                                                   >  2:980152/1‑150 (MQ=255)
                                              ccgccACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CAtg                                                                                                                     <  1:1177920/132‑1 (MQ=255)
                                              ccgccACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CAtg                                                                                                                     >  2:1177920/1‑132 (MQ=255)
                                               cgccACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAC‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGt                                                                                                 <  2:1531625/150‑1 (MQ=255)
                                                gccACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTa                                                                                                >  2:504024/1‑150 (MQ=255)
                                                   aCCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCa                                                                                             >  2:702234/1‑150 (MQ=255)
                                                        tGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCCTctgt                                                                                        <  1:1809164/150‑4 (MQ=255)
                                                        tGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTg                                                                                        <  1:504024/150‑1 (MQ=255)
                                                          ggCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCAA‑AATCGCCGTACCATCCTGGa                                                                                      <  2:1555219/150‑1 (MQ=255)
                                                             aTCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATaa                                                                                   >  1:1237566/1‑150 (MQ=255)
                                                                  tACCCAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATAACAGTCCCATTCTGGCTattggtt                                                                              <  2:118713/150‑7 (MQ=255)
                                                                  tACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCt                                                                              <  2:1217871/150‑1 (MQ=255)
                                                                  tACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCt                                                                              <  2:316832/150‑1 (MQ=255)
                                                                    ccAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTgg                                                                            >  2:421042/1‑150 (MQ=255)
                                                                     cAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGa                                                                           >  1:344282/1‑150 (MQ=255)
                                                                     cAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGa                                                                           <  1:1313387/150‑1 (MQ=255)
                                                                     cAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTCGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGa                                                                           >  1:1540941/1‑150 (MQ=255)
                                                                     cAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTCGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGa                                                                           >  1:1540952/1‑150 (MQ=255)
                                                                     cAAGGCATCGCCGCCATTGGCCACG‑ATTAACTGGATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGa                                                                           >  1:528068/1‑150 (MQ=255)
                                                                     cAAGGCATCGCCACCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGa                                                                           >  1:1147483/1‑150 (MQ=255)
                                                                          cATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGaaa                                                                      >  2:1063683/1‑150 (MQ=255)
                                                                            tCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGaaaaa                                                                    <  1:1129338/150‑1 (MQ=255)
                                                                            gcgccgcCATTGGCCACGCATTCGCT‑GATCGCAGGCTGGAACCCAAGGAGACTGCCGTAGCCGAAGATGTCCTTAAGACCCCGCCGAGCGTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGaaaaa                                                                    <  2:1348690/149‑1 (MQ=255)
                                                                               ccgccATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCaa                                                                                                                >  2:1908113/1‑104 (MQ=255)
                                                                               ccgccATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCaa                                                                                                                <  1:1908113/104‑1 (MQ=255)
                                                                                  ccatgcgCCACGCTTTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTCGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGAAAAAcccgcc                                                              <  2:1540941/144‑1 (MQ=255)
                                                                                  ccATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTCGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGAAAAAcccgcc                                                              <  2:1540952/150‑1 (MQ=255)
                                                                                    aTTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CAtgtg                                                                                                                   >  2:790876/1‑96 (MQ=255)
                                                                                    aTTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CAtgtg                                                                                                                   <  1:790876/96‑1 (MQ=255)
                                                                                    aTTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACCCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGAAAAAcccgcccc                                                            <  2:1904285/150‑1 (MQ=255)
                                                                                       ggCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGAAAAACCCGCCCCGca                                                         <  1:980152/150‑1 (MQ=255)
                                                                                                  tccgatctCGCTGCCTGGCGCCCACGGAAAGCGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CGTGTGCAACTCCA‑ACTTGTGGAATCTGCCTCTATAAAAGCTGGACTAAAAACCCGCCCCGCACACGCCAAAt                                               <  1:799283/143‑1 (MQ=255)
                                                                                                  cGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGAAAAACCCGCCCCGCACACGCCAAATg                                              >  1:1601052/1‑150 (MQ=255)
                                                                                                   gat‑gatCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCt                                                                              >  2:1518251/1‑117 (MQ=255)
                                                                                                   gat‑gatCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCt                                                                              <  1:1518251/117‑1 (MQ=255)
                                                                                                      tgctCGCTGCCCGGCGCGCACTGAAACTGTTGTAGCCGAAGATGTCCTTGAGAACGCGATGAGACT‑TTTGAGCCATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGAAAAACCCGCCCCGCACACGCCAAATGCGt                                           <  1:117739/147‑1 (MQ=255)
                                                                                                        atCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGAAAAACCCGCCCCGCACACGCCAAATGCGTaa                                         <  1:1719845/150‑1 (MQ=255)
                                                                                                          cGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGAAAAAcccgcccc                                                            >  2:1272426/1‑129 (MQ=255)
                                                                                                          cGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGAAAAAcccgcccc                                                            <  1:1272426/129‑1 (MQ=255)
                                                                                                                 tGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGAAAAACCCGCCCCGCACACGCCAAATGCGTAAGCGGttttt                                <  1:1610722/150‑1 (MQ=255)
                                                                                                                 tGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCAA‑TAGCTATTTACTATCCTGGATAAAGGCTGGACGAAAAACCCGCCCCGCACACGCCAAATGCGTAAGCGGttttt                                >  1:1772940/1‑150 (MQ=255)
                                                                                                                     gcCCACGGAAACTATCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGAAAAACCCGCCCCGCACACGCCAAATGCGTAAGCGGTTTTTGCCa                            <  2:1027851/150‑1 (MQ=255)
                                                                                                                       ccACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCGGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGAAAAACCCGCCCCGCACACGCCAAATGCGTAAGCGGTTTTTGCCAgg                          >  2:13144/1‑150 (MQ=255)
                                                                                                                         aCGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGAAAAACCCGCCCCGCACACGCCAAATGCGTAAGCGGTTTTTGCCAGGCa                        >  2:1688091/1‑150 (MQ=255)
                                                                                                                            gAAACTGTCGTAGCCGAAGATGTCATCGGGTACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGAAAAACCCGCCCCGCACACGCCAAATGCGTAAGCGGTTTTTGCCAGGCAGcc                     >  1:449512/1‑150 (MQ=255)
                                                                                                                             aaaCTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGAAAAACCCGCCCCGCACACGCCTAATGCGTAAGCGGTTTTTGCCAGGCAGCCa                    <  2:1237566/150‑1 (MQ=255)
                                                                                                                                 tGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGAAAAACCCGCCCCGCACACGc                                                    >  2:1277712/1‑114 (MQ=255)
                                                                                                                                 tGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGAAAAACCCGCCCCGCACACGCCAAATGCGTAAGCGGTTTTTGCCAGGCAGCCATTGa                >  1:1032417/1‑150 (MQ=255)
                                                                                                                                 tGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTAACCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGAAAAACCCGCCCCGCACACGc                                                    <  1:1277712/114‑1 (MQ=255)
                                                                                                                                   tCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGAAAAACCCGCCCCGCACACGCCAAATGCGTAAGCGGTTTTTGCCAGGCa                        >  2:820155/1‑140 (MQ=255)
                                                                                                                                   tCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGAAAAACCCGCCCCGCACACGCCAAATGCGTAAGCGGTTTTTGCCAGGCa                        <  1:820155/140‑1 (MQ=255)
                                                                                                                                    cGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGAAAAACCCGCCCCGCACACGCCAAATGCGTAAGCGGTTTTTGCCAGGCAGCCATTGAAAc             <  1:421042/150‑1 (MQ=255)
                                                                                                                                    cGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGAAAAACCCGCCCCGCACACGCCAAATGCGTAAGCGGTTTTTGCCAGGCAGCCATTGAAAc             <  1:197370/150‑1 (MQ=255)
                                                                                                                                     gTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGAAAAACCCGCCCCGCACACGCAGATGGCGTAAGCGGTTTTTGCCAGGCAGCCATTGAAAcc            >  1:98294/1‑150 (MQ=255)
                                                                                                                                        tccGAAGATGTCCTTGAGAACGCGCTGATCATGTTAGAG‑GATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGAAAAACCCGCCCCGCACACGCCAAATGCGTAAGCGGTTTTTGCCAGGCAGCCATTGAAACCTGc         <  2:98294/149‑1 (MQ=255)
                                                                                                                                         ccGAAGATGTCCTTGAGAACGCGCTCCACCTGCTCGAG‑CCTGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGAAAAACCCGCCCCGCACACGCCAAATGCGTAAGCGGTTTTTGCCAGGCAGCCATTGAAACCTGcc        <  1:942514/150‑1 (MQ=255)
                                                                                                                                          cGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGAAAAACCCGCCCCGCACACGCCAAATGCGTAAGCGGTTTTTGCCAGGCAGCCATTGAAACCTGccc       >  1:1315077/1‑150 (MQ=255)
                                                                                                                                          cGAAGAGGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGCACCATCCCGGAAAACGGCAAGACGAAAATACCGCCCCGGATTCGCCAATTGCTTAATGTGTGGTTGCCAGGCAGCCATTGAAACCTGccc       <  2:216839/150‑1 (MQ=255)
                                                                                                                                             aGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑GATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGAAAAACCCGCCCCGCACACGCCAAATGCGTAAGCGGTTTTTGCCAGGCAGCCATTGAAACCTGCCCCTg    <  2:1399511/150‑1 (MQ=255)
                                                                                                                                               aTGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAGCAGGATTATCG‑CTTACCATCCTGGATAAAGGCTGGACGAAAAACCCGCCCCGCACACGCCAAATGCGTAAGCGGTTTTTGCCAGGCAGCCATTGAAACCTGCCCCTGGa  <  2:528068/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                
CAAGCCCGGGCGCAGCAGCCCCGGCACCTGGAAACACAGCGATTTGCCGCCACCGGTGGGCATCAGTACCAAGGCATCGCCGCCATTGGCCACGCATTCGAT‑GATCGCTGCCTGGCGCCCACGGAAACTGTCGTAGCCGAAGATGTCCTTGAGAACGCGCTGAGCCTGTTCGAG‑CATGTGCAACTCCA‑AATCGCCGTACCATCCTGGATAAAGGCTGGACGAAAAACCCGCCCCGCACACGCCAAATGCGTAAGCGGTTTTTGCCAGGCAGCCATTGAAACCTGCCCCTGGA  >  NC_002947/5131275‑5131566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑