Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,182,971 G→C 7.3% H271D (CAT→GAT)  PP_4563 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,182,9710GC 7.3% 248.7 / 8.6 82H271D (CAT→GAT) PP_4563hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (34/42);  new base C (3/3);  total (37/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

TGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCT  >  NC_002947/5182825‑5183120
                                                                                                                                                  |                                                                                                                                                      
tGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGAt                                                                                                                                                     <  1:983714/150‑1 (MQ=255)
  gCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGa                                                                                                                                                   >  2:418113/1‑150 (MQ=255)
   cAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGAc                                                                                                                                                  <  1:1065540/150‑1 (MQ=255)
      gcgcCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGAt                                                                                                                                               <  1:1711147/150‑1 (MQ=255)
       cgccgcTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATc                                                                                                                                              <  2:1386255/150‑1 (MQ=255)
        gccgcTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGT‑AGGCCGTCAGGCTTTCTCGATGCAATCGTCATGGATGACGATCAg                                                                                                                                            >  1:560351/1‑150 (MQ=255)
          cgcTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATCCATGACGATcgca                                                                                                                                           <  1:243085/150‑4 (MQ=255)
           gcTCCACCAGCAGTCTGGCCATCAGCGCCGACCGTGACTTGTGGTTGCGCTACACCCTTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATCCATGACGATcgcat                                                                                                                                          <  2:682390/150‑5 (MQ=255)
             tCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGGCGATCAGGccc                                                                                                                                        <  2:469810/150‑1 (MQ=255)
              ccaccaTCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGT‑AGGCCGTCAGGCTTTCTCGATGCAATCGTCATGGATGACGATCAGGCCCTg                                                                                                                                      <  2:560351/150‑1 (MQ=255)
                  caTCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCtt                                                                                                                                   <  2:736121/150‑1 (MQ=255)
                     cAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGaa                                                                                                                                <  1:1106333/150‑1 (MQ=255)
                              ccTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGCTCAGGCCCTGCTTGAACAACCCGcc                                                                                                                       >  1:1437073/1‑150 (MQ=255)
                                  aGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATg                                                                                                                   <  2:1270607/150‑1 (MQ=255)
                                        gACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCcttc                                                                                                             >  2:624274/1‑150 (MQ=255)
                                           cGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTg                                                                                                          >  1:1027481/1‑150 (MQ=255)
                                             ggACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGaa                                                                                                        <  2:1780010/150‑1 (MQ=255)
                                                 tCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTg                                                                                                    >  2:1225496/1‑150 (MQ=255)
                                                  cGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGc                                                                                                   <  1:1681245/150‑1 (MQ=255)
                                                    cagctgctCGCCACCCGGACGGGTGTCGAGCAGCTGGACTTCCGCAACTTTGGTCATGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGccc                                                                                                 >  1:1078975/3‑150 (MQ=255)
                                                        tgcGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGc                                                                                             >  2:1326947/1‑150 (MQ=255)
                                                        tgcGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACACGCCAATGGCCTTCTTGAAGTTGCCCTTGc                                                                                             <  2:1117462/150‑1 (MQ=255)
                                                        tgcGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTCAGTACTGGCGTAAGGCCGTCAGGCTTTCTCGATGTGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGc                                                                                             <  1:592197/150‑1 (MQ=255)
                                                         gctcgccacCCGGACGGGTGTCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTAACTATACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCt                                                                                            >  1:959536/7‑150 (MQ=255)
                                                                  ccggacGGGTGTCGCGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTACTCGATGCGATCGGCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAAGGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑aa                                                                                  >  2:267780/6‑150 (MQ=255)
                                                                  ccGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTTGGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACGACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑aa                                                                                  <  1:1455028/150‑1 (MQ=255)
                                                                  ccGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCaa                                                                                                                     <  1:1552131/116‑1 (MQ=255)
                                                                  ccGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCaa                                                                                                                     >  2:1552131/1‑116 (MQ=255)
                                                                  ccGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑ac                                                                                  <  2:319962/150‑2 (MQ=255)
                                                                  caggtggggAGGCGTGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑aa                                                                                  <  2:1078975/144‑1 (MQ=255)
                                                                        gggTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCtt                                                                            <  1:401653/150‑1 (MQ=255)
                                                                           tGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCt                                                                         <  1:212421/150‑1 (MQ=255)
                                                                              cGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGAt                                                                      >  1:1443973/1‑150 (MQ=255)
                                                                                agcagcTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCa                                                                    >  1:707754/1‑150 (MQ=255)
                                                                                 gcagcTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCaa                                                                   <  1:758423/150‑1 (MQ=255)
                                                                                 gcagcTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCCATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCATTGCTGACGTTG‑AACAGCTTGCTGATCaa                                                                   <  1:624274/150‑1 (MQ=255)
                                                                                  cagcTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAAc                                                                  <  1:358862/150‑1 (MQ=255)
                                                                                     cTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑ATCAGCAAGCTGTTCAACgtc                                                               <  2:1027481/150‑3 (MQ=255)
                                                                                           ttCCGCAACTTCGTACAGGCGTACGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCa                                                         >  2:944362/1‑150 (MQ=255)
                                                                                           ttCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACg                                                                 >  2:26724/1‑142 (MQ=255)
                                                                                           ttCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACg                                                                 <  1:26724/142‑1 (MQ=255)
                                                                                           ttCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCa                                                         <  1:1730922/150‑1 (MQ=255)
                                                                                           ttCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCAATAGCTTACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCa                                                         >  1:1040189/1‑150 (MQ=255)
                                                                                           ttCCCCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCa                                                         <  2:580699/150‑1 (MQ=255)
                                                                                                cAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCtt                                                    <  1:463245/150‑1 (MQ=255)
                                                                                                  aCTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACACCCCGCTAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGt                                                  <  2:455790/150‑1 (MQ=255)
                                                                                                   cTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGTc                                                 <  1:422190/150‑1 (MQ=255)
                                                                                                   cTTCGTACAGGCGTAAGGCAGTAAGGCTTTATAGATGAGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGTc                                                 <  2:1040189/150‑1 (MQ=255)
                                                                                                    ttCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCg                                                >  2:1258291/1‑150 (MQ=255)
                                                                                                    ttCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCg                                                >  2:843945/1‑150 (MQ=255)
                                                                                                       gTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGcac                                             >  2:183856/1‑150 (MQ=255)
                                                                                                        tACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCt                                                                                            >  1:875971/1‑103 (MQ=255)
                                                                                                        tACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCt                                                                                            <  2:875971/103‑1 (MQ=255)
                                                                                                            ggCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACAcccc                                        >  1:598814/1‑150 (MQ=255)
                                                                                                             gCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACAccccc                                       >  2:1279017/1‑150 (MQ=255)
                                                                                                               gTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCaa                                     >  2:1740028/1‑150 (MQ=255)
                                                                                                                  aGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGTGGCACACCCCCAacac                                  >  1:235737/1‑150 (MQ=255)
                                                                                                                          aGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCtt                          >  1:803710/1‑150 (MQ=255)
                                                                                                                           ggCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCATGCTGACGTTGAAACCGCGT‑ATCAGCTTGTGAGGTTCGCTCTTGTCGCACACCCCCAACACCCCGCCTTc                         <  1:1279017/150‑1 (MQ=255)
                                                                                                                              tttCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGc                      >  2:80798/1‑150 (MQ=255)
                                                                                                                                  tcGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTcc                  <  1:318366/150‑1 (MQ=255)
                                                                                                                                  tcGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTcc                  >  1:754614/1‑150 (MQ=255)
                                                                                                                                   cGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCa                 <  2:233935/150‑1 (MQ=255)
                                                                                                                                   cGATGCGATCGTCATGGATGACGATCAGGCACTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTCG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCa                 <  2:1094650/150‑1 (MQ=255)
                                                                                                                                    gATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGcc                                                                                                  <  1:734005/69‑1 (MQ=255)
                                                                                                                                    gATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGcc                                                                                                  >  2:734005/1‑69 (MQ=255)
                                                                                                                                    gATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAg                >  2:1677595/1‑150 (MQ=255)
                                                                                                                                    gATGCGATCGTCATGGATGACAATCAAGCGCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGGTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGAAGGGTACCTCTTGTCGAAAACCCCCAACACCCCGCCTTCGGCTTcacg                >  2:225796/1‑147 (MQ=255)
                                                                                                                                      cctgATCGTCATCCATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGgc              >  1:259747/4‑150 (MQ=255)
                                                                                                                                       ctgATCGTCATCCATGACGATCGGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTc                         <  2:1810026/136‑1 (MQ=255)
                                                                                                                                       ctgATCGTCATCCATGACGATCGGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTc                         >  1:1810026/3‑138 (MQ=255)
                                                                                                                                         gATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑ATCAGCAAGCTGTTCAAc                                                                  >  2:1546889/1‑95 (MQ=255)
                                                                                                                                         gATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑ATCAGCAAGCTGTTCAAc                                                                  <  1:1546889/95‑1 (MQ=255)
                                                                                                                                         gATCGTCATCCATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGgcgcg           >  1:316189/1‑150 (MQ=255)
                                                                                                                                          aTCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGtaaat                                                       >  1:1374271/1‑101 (MQ=255)
                                                                                                                                          aTCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCAct                                                       <  2:1374271/105‑1 (MQ=255)
                                                                                                                                          aTCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGgcgcgc          <  2:1738461/150‑1 (MQ=255)
                                                                                                                                              tCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATg      >  2:327858/1‑150 (MQ=255)
                                                                                                                                               cATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGa     <  2:707754/150‑1 (MQ=255)
                                                                                                                                               cATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGa     <  2:1443973/150‑1 (MQ=255)
                                                                                                                                                aTGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGAt    >  2:1598213/1‑150 (MQ=255)
                                                                                                                                                 tGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATc   <  1:327858/150‑1 (MQ=255)
                                                                                                                                                  ggATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCt  >  2:1094031/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                      
TGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTG‑AACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGATCT  >  NC_002947/5182825‑5183120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑