Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,184,168 A→G 7.2% D86G (GAC→GGC)  PP_4564 → lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,184,1680AG 7.2% 311.1 / 10.0 110D86G (GAC→GGC) PP_4564lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (49/53);  new base G (2/6);  total (51/59)
Fisher's exact test for biased strand distribution p-value = 2.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.54e-01

Only 100 of 120 total aligned reads displayed.

GCCGTGGTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAGGCCTTCC  >  NC_002947/5184019‑5184317
                                                                                                                                                      |                                                                                                                                                     
gCCGTGGTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCcga                                                                                                                                                       >  1:1107520/1‑150 (MQ=255)
  ggtagTCGCTCAGCGACTACCTGGACATGAACCGAGAGGCTCGACGCGCT‑CGAGGGGATCGGTTGCGCGTTGGCCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCcgacg                                                                                                                                                     >  1:1279201/5‑150 (MQ=255)
   gTGGTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCcgacga                                                                                                                                                    >  1:986670/1‑150 (MQ=255)
   gTGGTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCcgacga                                                                                                                                                    <  1:1461307/150‑1 (MQ=255)
    tGGTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCgacgac                                                                                                                                                   >  2:636866/1‑150 (MQ=255)
      gTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCa                                                                                                                                                 <  1:1661394/150‑1 (MQ=255)
      gTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCa                                                                                                                                                 >  2:1122102/1‑150 (MQ=255)
       tCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAg                                                                                                                                                <  2:547436/150‑1 (MQ=255)
       tCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAG‑GGCGCTGCTGGATGAACGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAg                                                                                                                                                <  1:1218999/150‑1 (MQ=255)
         gatGAGCGACTACCTGGACATGAACCGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGt                                                                                                                                              >  1:1348253/3‑150 (MQ=255)
         gCTGAGCGACTACCTGGACATGAACCGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCTTGGCATTGCAAGACTCAACTGCCCGGCTACCGCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGt                                                                                                                                              <  2:153955/150‑1 (MQ=255)
         gCTGAGCGACTACCTGGACATGAACCGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGCCCGGCGACGTTACATGGTCGCCTACGACCAggg                                                                                                                                              >  2:1361560/1‑149 (MQ=255)
         gCTGAGCGACTACCTGGACATGAACCGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACCTGGTCGTCGGCGACCATGt                                                                                                                                              <  2:1061433/150‑1 (MQ=255)
         gCTGAGCGACTACCTGGACATGAACCGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGt                                                                                                                                              <  1:1271014/150‑1 (MQ=255)
         gCTGAGCGACTACCTGGACATGAACAGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACCTGGTCGTCGGCGACCATGt                                                                                                                                              <  2:1880970/150‑1 (MQ=255)
          cTGAGCGACTACCTGGACATGAACCGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCCTAGCAAACCTACACAGCCCGACCAGTACGACTGGGTTGACCGTCTACGTGACCTGGTCGTCGGCGACCATGTc                                                                                                                                             <  1:513952/150‑1 (MQ=255)
           tGAGCGACTACCTGGACATGAACCGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCa                                                                                                                                            >  2:1566369/1‑150 (MQ=255)
            gAGCGACTACCTGGACATGAACCGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACCTGGTCGTCGGCGACCATGTCAc                                                                                                                                           <  2:1258540/150‑1 (MQ=255)
            gAGCGACTACCTGGACATGAACCGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCAc                                                                                                                                           <  2:901903/150‑1 (MQ=255)
             aGCGACTACCTGGACATGAACCGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCAcc                                                                                                                                          <  1:636866/150‑1 (MQ=255)
              gCGACTACCTGGACATGAACCGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCg                                                                                                                                         >  2:1812749/1‑150 (MQ=255)
                      cTGGACATGACCCGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGcc                                                                                                                                 <  1:770702/150‑1 (MQ=255)
                      cTGGACATGAACCGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGcc                                                                                                                                 >  1:1209856/1‑150 (MQ=255)
                      cTGGACATGAACCGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGcc                                                                                                                                 >  1:651761/1‑150 (MQ=255)
                       tGGACATGAACCGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGccc                                                                                                                                >  1:1015059/1‑150 (MQ=255)
                           caggAACCGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTgca                                                                                                                            <  2:1180170/147‑1 (MQ=255)
                           cATGAACCGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTgca                                                                                                                            >  2:504189/1‑150 (MQ=255)
                            aTGAACCGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGATCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTgcag                                                                                                                           >  2:1156034/1‑150 (MQ=255)
                                aCCGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCaaa                                                                                                                       <  1:155186/150‑1 (MQ=255)
                                 ccGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAg                                                                                                                      >  2:1914070/1‑150 (MQ=255)
                                  cgcgAGCAAAG‑GGCGCTGCTGGATGATCGTTAGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACCTGGTCGTCGGCGACCat                                                                                                                                                <  1:498601/123‑2 (MQ=255)
                                  cgcgAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACCTGGTCGTCGGCGACCat                                                                                                                                                >  2:498601/1‑122 (MQ=255)
                                   gcgAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCa                                                                                                                    >  1:1633108/1‑150 (MQ=255)
                                     gAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCAcc                                                                                                                  <  1:1122102/150‑1 (MQ=255)
                                                cgcTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTcc                                                                                                        <  1:522496/150‑1 (MQ=255)
                                                cgcTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTcc                                                                                                        <  1:793409/150‑1 (MQ=255)
                                                  ctgctgGATGATCGGTTGCGCGCGCACCTGGCCTGGCCTTCTACGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGt                                                                                                      <  1:679848/150‑1 (MQ=255)
                                                   tgctgGATGATCGGTTGCGCGAGAACGTTGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTg                                                                                                     >  1:1697525/1‑150 (MQ=255)
                                                      tgGATGAGCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAgg                                                                                                  <  2:1209856/150‑1 (MQ=255)
                                                       ggATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGc                                                                                                 >  2:1500779/1‑150 (MQ=255)
                                                            atCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAATCACCCGCGAGGTCCGTGAGGCAATTg                                                                                            <  1:991395/150‑1 (MQ=255)
                                                            atCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTg                                                                                            <  1:991264/150‑1 (MQ=255)
                                                             tCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATCgg                                                                                           >  2:244199/1‑150 (MQ=255)
                                                               ggTTGCGCGAGCACCTGGCCTGGGATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTc                                                                                         >  2:949107/1‑150 (MQ=255)
                                                                gTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGATCg                                                                                        >  1:27098/1‑150 (MQ=255)
                                                                   gcgcGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGACTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGAtt                                                                                     >  2:175175/1‑150 (MQ=255)
                                                                         gCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTAACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGa                                                                               >  1:1584892/1‑150 (MQ=255)
                                                                          cACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTAACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAg                                                                              <  2:1584892/150‑1 (MQ=255)
                                                                           aCCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAgg                                                                             >  2:1168349/1‑150 (MQ=255)
                                                                                gcctggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCAGCGGAAATc                                                                        <  2:27098/150‑1 (MQ=255)
                                                                                 cctggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCCAGGAAATCa                                                                       >  2:767166/1‑150 (MQ=255)
                                                                                  ctggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCAc                                                                      >  1:1697135/1‑150 (MQ=255)
                                                                                  ctggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCAc                                                                      >  1:176787/1‑150 (MQ=255)
                                                                                    ggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGACCGTCAGGCAATCGGTCGATTGGCCGAGGAAATCAccc                                                                    >  2:627666/1‑150 (MQ=255)
                                                                                          gCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGGCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGGCCGTGAGACAATTCGTCGATTGGCCGAGGAAAGCACCCCTTCgg                                                              >  2:422244/1‑150 (MQ=255)
                                                                                           cAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGc                                                             >  2:140501/1‑150 (MQ=255)
                                                                                           cAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGc                                                             >  1:1098236/1‑150 (MQ=255)
                                                                                           cAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGc                                                             <  1:1914070/150‑1 (MQ=255)
                                                                                            aaGACTCAACTGCCCGGCTACCTCGACTTGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGcc                                                            >  2:351458/1‑150 (MQ=255)
                                                                                            aaGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCa                                                                                                                                     <  1:1885496/77‑1 (MQ=255)
                                                                                            aaGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCa                                                                                                                                     >  2:1885496/1‑77 (MQ=255)
                                                                                                cTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCg                                                        >  1:1320245/1‑150 (MQ=255)
                                                                                                   aaCTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCg                                                               <  2:620154/140‑1 (MQ=255)
                                                                                                   aaCTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCg                                                               >  1:620154/1‑140 (MQ=255)
                                                                                                   aaCTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAAc                                                     >  2:859053/1‑150 (MQ=255)
                                                                                                       gCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCaaa                                                                                                                       <  2:461947/80‑1 (MQ=255)
                                                                                                       gCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCaaa                                                                                                                       >  1:461947/1‑80 (MQ=255)
                                                                                                           ggCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCg                                                               >  1:674850/1‑132 (MQ=255)
                                                                                                           ggCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTgcgc                                             <  1:859053/150‑1 (MQ=255)
                                                                                                                 cTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATg                                       >  1:1557871/1‑150 (MQ=255)
                                                                                                                  tCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGt                                                                                          >  2:1150486/1‑98 (MQ=255)
                                                                                                                  tCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGt                                                                                          <  1:1150486/98‑1 (MQ=255)
                                                                                                                  tCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGaa                                                      >  1:1164032/1‑134 (MQ=255)
                                                                                                                  tCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGa                                      <  2:1107520/150‑1 (MQ=255)
                                                                                                                  tCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGa                                      >  1:1184178/1‑150 (MQ=255)
                                                                                                                  tCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGa                                      >  2:1726655/1‑150 (MQ=255)
                                                                                                                   cGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCGCAGGCCACCGAACTGCTGCGCGGCATGAg                                     <  1:989194/150‑1 (MQ=255)
                                                                                                                    gACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGc                                    <  2:1633108/150‑1 (MQ=255)
                                                                                                                    gACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGc                                    <  1:1582360/150‑1 (MQ=255)
                                                                                                                      cTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGa                                  <  1:627666/150‑1 (MQ=255)
                                                                                                                         gCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACaa                               <  2:1697135/150‑1 (MQ=255)
                                                                                                                          cTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTc                                                                                         >  1:671488/1‑91 (MQ=255)
                                                                                                                          cTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTc                                                                                         <  2:671488/91‑1 (MQ=255)
                                                                                                                           tCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGaa                                                      >  1:585627/1‑125 (MQ=255)
                                                                                                                            cGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCa                            <  1:472314/150‑1 (MQ=255)
                                                                                                                                cGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCACGGCATGGACGACAACCAGGTa                        >  2:439112/1‑150 (MQ=255)
                                                                                                                                  gCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAActgct                                                 <  2:1637843/123‑1 (MQ=255)
                                                                                                                                  gCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAActgct                                                 >  1:1637843/1‑123 (MQ=255)
                                                                                                                                       gTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGa                 <  2:1320245/150‑1 (MQ=255)
                                                                                                                                          aCATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGc              >  2:1319387/1‑150 (MQ=255)
                                                                                                                                          aCATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGAAGACGGGCCGAGGAGAAGACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGc              <  1:1178716/150‑1 (MQ=255)
                                                                                                                                            ctGGTCGTCGGCGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAAt                                                                         <  1:1910750/88‑1 (MQ=255)
                                                                                                                                            aTGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATNCGt            <  2:1194136/150‑1 (MQ=255)
                                                                                                                                            aTGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGt            <  2:12390/150‑1 (MQ=255)
                                                                                                                                              ggTCGCCGACGCACAGTTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGa          <  2:986670/150‑1 (MQ=255)
                                                                                                                                              ggTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGa          <  1:1361560/150‑1 (MQ=255)
                                                                                                                                                tCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAgg        >  2:1331195/1‑150 (MQ=255)
                                                                                                                                                   ccGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGa                                                                                       >  2:666883/1‑68 (MQ=255)
                                                                                                                                                    cgacgaCCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAGGCCtt    >  2:1465074/1‑150 (MQ=255)
                                                                                                                                                      acgacCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAGGCCTTcc  <  2:1279201/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GCCGTGGTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAG‑GGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAGGCCTTCC  >  NC_002947/5184019‑5184317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑