Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,332,293 C→G 11.1% intergenic (+28/‑21) dksA → / → gluQ RNA polymerase‑binding transcription factor DksA/glutamyl‑Q tRNA(Asp) synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,332,2930CG11.1% 156.9 / 10.8 55intergenic (+28/‑21)dksA/gluQRNA polymerase‑binding transcription factor DksA/glutamyl‑Q tRNA(Asp) synthetase
Reads supporting (aligned to +/- strand):  ref base C (30/18);  new base G (4/2);  total (34/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

TCGTGCGGCATCGAGATCGGCCTGCGCCGCCTGGAAGCCCGCCCTACCGCCGACCTGTGCTTCGACTGCAAGGAAATTGCCGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGCCCCCACCCCCAGCGGCTTCCTGCACTTCGGCTCGCTGGTCGCCGCCCTCGCCTCCTGGCTCGATGCCCGCGCCGTCAAC  >  NC_002947/5332152‑5332439
                                                                                                                                             |                                                                                                                                                  
tCGTGCGGCATCGAGATCGGCCTGCGCCGCCTGGAAGCCCGCCCTACCGCCGACCTGTGCTTCGACTGCAAGGAAATTGCCGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGccc                                                                                                                                            <  2:383643/150‑1 (MQ=255)
  gTGCGGCATCGAGATCGGCCTGCGCCGCCTGGAAGCCCGCCCTACCGCCGACCTGTGCTTCGACTGCAAGGAAATTGCCGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCg                                                                                                                                          >  1:472639/1‑150 (MQ=255)
   tGCGGCATCGAGATCGGCCTGCGCCGCCTGGAAGCCCGCCCTACAGCCGACCTGTGCTTCGACTGCAAGGAAATTGCCGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGt                                                                                                                                         <  1:511072/150‑1 (MQ=255)
         aTCGAGATCGGCCTGCGCCGCCTGGAAGCCCGCCCTACCGCCGACCTGTGCTTCGACTGCAAGGAAATTGCCGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATTATGCGCCCCGTTTCAtt                                                                                                                                   <  2:1815728/150‑1 (MQ=255)
          tCGAGATCGGCCTGCGCCGCCTGGAAGCCCGCCCTACCGCCGACCTGTGCTTCGACTGCAAGGAAATTGCCGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATGATGCGcc                                                                                                                                             >  1:997555/1‑139 (MQ=255)
          tCGAGATCGGCCTGCGCCGCCTGGAAGCCCGCCCTACCGCCGACCTGTGCTTCGACTGCAAGGAAATTGCCGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCATCCACCTGAACGGGGCGCATGATGCGcc                                                                                                                                             <  2:997555/139‑1 (MQ=255)
              gaTCGGCCTGCGCCGCCTGGAAGCCCGCCCTACCGCCGACCTGTGCTTCGACTGCAAGGAAATTGCCGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTatat                                                                                                                              >  1:867112/1‑150 (MQ=255)
                tCGGCCTGCGCCGCCTGGAAGCCCGCCCTACCGCCGACCTGTGCTTCGACTGCAAGGAAATTGCCGAGAAAAAGGAAAAAACGGTCGGCAATGGTTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGc                                                                                                                            <  2:1205903/150‑1 (MQ=255)
                             ccTGGAAGCCCGCCCTACCGCCGACCTGTGCTTCGACTGCAAGGAAATTGCCGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCAt                                                                                                               >  1:1850397/1‑150 (MQ=255)
                               tGGAAGCCCGCCCTACCGCCGACCTGTGCTTCGACTGCAAGGAAATTGCCGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGa                                                                                                             <  1:1396332/150‑1 (MQ=255)
                               tGGAAGCCCGCCCTACCGCCGACCTGTGCTTCGACTGCAAGGAAATTGCCGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGa                                                                                                             >  1:300050/1‑150 (MQ=255)
                               cggAAGCCCGCCCTCCCGCCGACCTGTGCTTCGACTGCAAGGAAATTGCCGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGa                                                                                                             <  1:381561/149‑1 (MQ=255)
                                      ccgcccTACCGCCGACCTGTGCTTCGACTGCAAGGAAATTGCCGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTc                                                                                                      >  2:604690/1‑150 (MQ=255)
                                          ccTACCGCCGACCTGTGCTTCGACTGCAAGGAAATTGCCGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGc                                                                                                  <  1:1730287/150‑1 (MQ=255)
                                            tACCGCCGACCTGTGCTTCGACTGCAAGGAAATTGCCGAGAAAAAGGAACACACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTa                                                                                                >  2:927746/1‑150 (MQ=255)
                                              ccgccgACCTGTGCTTCGACTGCAAGGAAATTGCCGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACa                                                                                              >  1:277161/1‑150 (MQ=255)
                                              ccgccgACCTGTGCTTCGACTGCAAGGAAATTGCCGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACa                                                                                              >  2:1300115/1‑150 (MQ=255)
                                                     ccTGTGCTTCGACTGCAAGGAAATTGCCGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGgcg                                                                                       <  2:867112/150‑1 (MQ=255)
                                                                aCTGCAAGGAAATTGCCGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGccccca                                                                            <  2:1686730/150‑1 (MQ=255)
                                                                  tGCAAGGAAATTGCCGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATc                                                                                            <  2:215191/132‑1 (MQ=255)
                                                                  tGCAAGGAAATTGCCGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATc                                                                                            >  1:215191/1‑132 (MQ=255)
                                                                    cAAGGAAATTGCCGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGcccccacccc                                                                        >  2:1711367/1‑150 (MQ=255)
                                                                    cAAGGAAATTGCCGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGcccccacccc                                                                        <  1:927746/150‑1 (MQ=255)
                                                                    cAAGGAAATTGCCGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGcccccacccc                                                                        >  2:1009056/1‑150 (MQ=255)
                                                                    cAAGGAAATTGCCGAGAAAAAGGAAAAAACGGTCGACAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGcccccacccc                                                                        >  2:1011325/1‑150 (MQ=255)
                                                                        gAAATTGCCGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGc                                                                                                  <  1:86374/120‑1 (MQ=255)
                                                                        gAAATTGCCGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGc                                                                                                  >  2:86374/1‑120 (MQ=255)
                                                                           aTTGCCGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGCCCCCACCCCCAGCGGc                                                                 >  1:1391418/1‑150 (MQ=255)
                                                                               ccGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACAATCACCGACTCCAGCTACATCGGGCGCTTCGCCCCCACCCCCAGCGGCTTcc                                                             >  2:348614/1‑150 (MQ=255)
                                                                                cGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGCCCCCACCCCCAGCGGCTTCCt                                                            >  1:1748158/1‑150 (MQ=255)
                                                                                cGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGCCCCCACCCCCAGCGGCTTCCt                                                            >  2:86458/1‑150 (MQ=255)
                                                                                agagaAAAAGGAAAAAACGGTCGGCCAACGCTGACCTTCCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGCCCCCACCCCCAGCGGCTTCCt                                                            >  2:1168835/2‑150 (MQ=255)
                                                                                 gagaAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGcccc                                                                              <  2:1537671/131‑1 (MQ=255)
                                                                                 gagaAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGcccc                                                                              >  1:1537671/1‑131 (MQ=255)
                                                                                 gagaAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGCCCCCACCCCCAGCGGCTTCCTg                                                           >  2:339958/1‑150 (MQ=255)
                                                                                 gagaAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGCCCCCACCCCCAGCGGCTTCCTg                                                           >  2:767519/1‑150 (MQ=255)
                                                                                    aaaaaGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGCCCCCACCCCCAGCGGCTTCCTGCAc                                                        <  1:1690665/150‑1 (MQ=255)
                                                                                      aaaGGATAAGACGGTCGTGAAAGGCTGATCTTTCTACCACCTGAACGGGGCGCATCATGCGCACCGTATCATTTATCTGCCCAGCCTGACTATGACCGACACCAGCTACATAGGGCGCTTCGCCCTCACCGGCGGCGGCGTCCTGCACtt                                                      >  2:330686/1‑150 (MQ=255)
                                                                                      aaaGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGCCCCCACCCCCAGCGGCTTCCTGCACtt                                                      >  2:696998/1‑150 (MQ=255)
                                                                                        aGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGCCCCCACCCCCAGCGGCTTCCTGCACTTCg                                                    >  2:444996/1‑150 (MQ=255)
                                                                                              aaaCGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGCCCCCACCCCCAGCGGCTTCCTGCACTTCGGCTCGc                                              <  2:1383655/150‑1 (MQ=255)
                                                                                                       gCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGCCCCCACCCCCAGCGGCTTCCt                                                            >  2:1568137/1‑127 (MQ=255)
                                                                                                       gCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGCCCCCACCCCCAGCGGCTTCCt                                                            <  1:1568137/127‑1 (MQ=255)
                                                                                                            ggCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGCCCCCACCCCCAGCGGCTTCCTGCACTTCGGCTCGCTGGTCGCCGCCCTc                                <  1:1009056/150‑1 (MQ=255)
                                                                                                                 aTCTTTCTTCCCCCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGCCCCCACCCCCAGCGGCTTCCTGCACTTCGGCTCGCTGGTCGCCGCCCTCGcctc                           >  2:1221857/1‑150 (MQ=255)
                                                                                                                  tctttcttCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGCCCCCACCCCCAGc                                                                    <  2:1241684/108‑1 (MQ=255)
                                                                                                                  tctttcttCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGCCCCCACCCCCAGc                                                                    >  1:1241684/1‑108 (MQ=255)
                                                                                                                        ttcCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGCCCCCACCCCCAGCGGCTTCCTGCACTTCGGCTCGCTGGTCGCCGCCCTCGCCTCCTCGCtg                    <  2:472639/150‑2 (MQ=255)
                                                                                                                                  aCGGGGCGCATGATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGCCCCCACCCCCAGCGGCTTCCTGCACTTCGGCt                                                 <  2:56864/111‑1 (MQ=255)
                                                                                                                                  aCGGGGCGCATGATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGCCCCCACCCCCAGCGGCTTCCTGCACTTCGGCt                                                 >  1:56864/1‑111 (MQ=255)
                                                                                                                                    ggggCGCATGATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGCCCCCACCCCCAGCGGCTTCCTGCACTTCGGCTCGCTGGTCGCCGCCCTCGCCTCCTGGCTCGATGCCCGCGcc        >  2:1662997/1‑150 (MQ=255)
                                                                                                                                     gggCGCATGATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGCCCCCACCCCCAGCGGCTTCCTGCACTTCGGCTCGCTGGTCGCCGCCCTCGCCTCCTGGCTCGATGCCCGCGCCg       >  1:1266208/1‑150 (MQ=255)
                                                                                                                                         gcATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGCCCCCACCCCCAGCGGCTTCCTGCACTTCGGCTCGCTGGTCGCCGCCCTCGCCTCCTGGCTCGATGCCCGCGCCGTCaa   >  2:1384467/1‑150 (MQ=255)
                                                                                                                                         gcATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGCCCCCACCCCCAGCGGCTTCCTGCACTTCGGCTCGCTGGTCGCCGCCCTCGCCTCCTGGCTCGATGCCCGCGCCGTCaa   >  1:362987/1‑150 (MQ=255)
                                                                                                                                         gcATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAAATCCATAGGGCGCTTAAAACGGACCCTCAGCGGCTTCCTGCACTTCGGCTCGCTGGTCGCCGCCCTCGCCTCCTGGCTCGATGCCCGCGCCGTCaa   >  1:705228/1‑150 (MQ=255)
                                                                                                                                          catgatGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGCCCCCACCCCCAGCGGCTTCCTGCACTTCGGCTCGCTGGTCGCCGCCCTCGCCTCCTGGCTCGATGCCCGCGCCGTCAAc  <  1:1662997/150‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
TCGTGCGGCATCGAGATCGGCCTGCGCCGCCTGGAAGCCCGCCCTACCGCCGACCTGTGCTTCGACTGCAAGGAAATTGCCGAGAAAAAGGAAAAAACGGTCGGCAAAGGCTGATCTTTCTTCCACCTGAACGGGGCGCATCATGCGCCCCGTTTCATTTATATGCCCAGCCTGACCATGACCGACTCCAGCTACATCGGGCGCTTCGCCCCCACCCCCAGCGGCTTCCTGCACTTCGGCTCGCTGGTCGCCGCCCTCGCCTCCTGGCTCGATGCCCGCGCCGTCAAC  >  NC_002947/5332152‑5332439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑