Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,336,227 G→A 5.5% R870H (CGC→CAC)  PP_4695 → two‑component system sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,336,2270GA 5.5% 265.4 / 4.1 91R870H (CGC→CAC) PP_4695two‑component system sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (48/38);  new base A (2/3);  total (50/41)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

GGCAGCCACCAGAACAGCGAGGAGCCCGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGATTCCGAAGAACATCATG  >  NC_002947/5336079‑5336375
                                                                                                                                                    |                                                                                                                                                    
ggcAGCCACCAGAACAGCGAGGAGCCCGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGACCAAATACGAATTCGAAGTACAGTTCTTCAAACTTTGCGCTCCTGCCCATTGGGCCGAAGGTGACCCGCAgcgc                                                                                                                                                     >  1:329598/1‑150 (MQ=255)
   aGCCACCAGAACAGCGAGGAGCCCGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACACGGAACATTGGGCCGAAGGTGACCCGCAGCGCCTg                                                                                                                                                  >  1:488989/1‑150 (MQ=255)
    gCCACCAGAACAGCGAGGAGCCCGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTgg                                                                                                                                                 <  1:1519266/150‑1 (MQ=255)
      caccaGAACAGCGAGGAGCCCGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGcc                                                                                                                                               >  1:718718/1‑150 (MQ=255)
       accaGAACAGCGAGGAGCCCGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGccc                                                                                                                                              >  1:1035086/1‑150 (MQ=255)
        ccaGAACAGCGAGGAGCCCGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCa                                                                                                                                             <  1:946101/150‑1 (MQ=255)
              cAGCGAGGAGCCGGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATCTCGAAGTACAGTTCTTCAACCCTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCACCTGGGCCAGGCGCt                                                                                                                                       <  2:484558/150‑1 (MQ=255)
                gCGAGGAGCCCGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGa                                                                                                                                     <  1:1916424/150‑1 (MQ=255)
                gCGAGGAGCCCGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGa                                                                                                                                     <  2:1778223/150‑1 (MQ=255)
                gCGAGGAGCCCGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGa                                                                                                                                     <  2:1670667/150‑1 (MQ=255)
                    ggagCCCGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCaa                                                                                                                                 >  1:1577988/1‑150 (MQ=255)
                      agCCCGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAAcc                                                                                                                               <  1:160524/150‑1 (MQ=255)
                        cccGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTg                                                                                                                             >  1:952585/1‑150 (MQ=255)
                         ccGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGAACAACCTGc                                                                                                                            >  2:1540652/1‑150 (MQ=255)
                          cGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGct                                                                                                                           >  2:1379191/1‑150 (MQ=255)
                             gtgCCTGGCTGAAGTGGCACCACATTCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGTCCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCtctc                                                                                                                        <  2:142949/150‑1 (MQ=255)
                                ccTGGCTGAAGTGGCACAAGATGCCATCGGTCTACTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCaa                                                                                                                     >  2:1656693/1‑150 (MQ=255)
                                  tGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACg                                                                                                                   <  2:75864/150‑1 (MQ=255)
                                  tGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACg                                                                                                                   >  1:302383/1‑150 (MQ=255)
                                         aGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGa                                                                                                            <  2:1604051/150‑1 (MQ=255)
                                               acaAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTc                                                                                                      <  2:1739323/150‑1 (MQ=255)
                                               acaAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTGCAACCTTTGCGACCCGGAACCTTGGGTCGAATGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTc                                                                                                      >  1:1875419/1‑150 (MQ=255)
                                                caAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTcg                                                                                                     <  1:1727139/150‑1 (MQ=255)
                                                caAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTcg                                                                                                     <  1:1379191/150‑1 (MQ=255)
                                                  aGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTcgcc                                                                                                   <  1:456232/150‑1 (MQ=255)
                                                    aTGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCTATGCCTcgccgc                                                                                                 >  1:389347/1‑150 (MQ=255)
                                                        cATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCgg                                                                                             >  2:434372/1‑150 (MQ=255)
                                                        cATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCgg                                                                                             >  2:187122/1‑150 (MQ=255)
                                                        cATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTATCCGGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCgg                                                                                             >  1:118352/1‑150 (MQ=255)
                                                         aTCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGc                                                                                            >  2:660841/1‑150 (MQ=255)
                                                         aTCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGc                                                                                            >  2:1273845/1‑150 (MQ=255)
                                                         aTCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGc                                                                                            >  2:251574/1‑150 (MQ=255)
                                                           cGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCgccgcc                                                                                                >  2:6793/1‑144 (MQ=255)
                                                           cGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCgccgcc                                                                                                <  1:6793/144‑1 (MQ=255)
                                                           cGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAg                                                                                          <  1:1759540/150‑1 (MQ=255)
                                                            ggTCTGCTGGCGTTGAACCGGCGCAATTTCAAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCCGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAgc                                                                                         <  2:1816285/150‑1 (MQ=255)
                                                                tgctgGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTGCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCcg                                                                                     >  2:1338485/1‑150 (MQ=255)
                                                                       gTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCcgtgcgcgt                                                                              <  1:1656693/150‑1 (MQ=255)
                                                                           aaCCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCCGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCgtgcgcgtgcgc                                                                          >  1:1543179/1‑150 (MQ=255)
                                                                            aCCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCa                                                                         <  2:1577988/150‑1 (MQ=255)
                                                                              cGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGc                                                                       <  2:1322000/150‑1 (MQ=255)
                                                                                gcgcAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGa                                                                     <  1:819675/150‑1 (MQ=255)
                                                                                gcgcAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGa                                                                     >  1:1364822/1‑150 (MQ=255)
                                                                                      tttCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTaa                                                               <  1:187122/150‑1 (MQ=255)
                                                                                       ttCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAAc                                                              <  2:622517/150‑1 (MQ=255)
                                                                                       ttCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCACCTgggc                                                                                                                                               <  1:537371/69‑3 (MQ=255)
                                                                                       ttCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCACCTgggc                                                                                                                                               >  2:537371/1‑67 (MQ=255)
                                                                                          gAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAg                                                           <  2:329598/150‑1 (MQ=255)
                                                                                          gAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAg                                                           >  1:776582/1‑150 (MQ=255)
                                                                                                 aGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCg                                                    <  2:146235/150‑1 (MQ=255)
                                                                                                 aGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCg                                                    >  2:968671/1‑150 (MQ=255)
                                                                                                 aGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCg                                                    >  1:1236070/1‑150 (MQ=255)
                                                                                                  gTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCTCCGTTGGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGt                                                   >  1:1153564/1‑150 (MQ=255)
                                                                                                      ttcAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGAc                                               >  1:480413/1‑150 (MQ=255)
                                                                                                        cAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCt                                             >  1:1831573/1‑150 (MQ=255)
                                                                                                         aaCCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTg                                            >  1:900821/1‑150 (MQ=255)
                                                                                                            cTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCacac                                                      >  1:1530467/1‑137 (MQ=255)
                                                                                                            cTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCacac                                                      <  2:1530467/137‑1 (MQ=255)
                                                                                                            cTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGAtc                                         >  2:638779/1‑150 (MQ=255)
                                                                                                            cTTTGCGACCCGGACCATTGGGCCGAAGGTGACCAGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGAtc                                         >  1:1315918/1‑150 (MQ=255)
                                                                                                             tttGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGTGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGAtcg                                        >  1:216028/1‑150 (MQ=255)
                                                                                                                  gACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGaa                                   <  1:660841/150‑1 (MQ=255)
                                                                                                                  gACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGaa                                   >  2:302531/1‑150 (MQ=255)
                                                                                                                  gACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGaa                                   <  2:1587666/150‑1 (MQ=255)
                                                                                                                   aCCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGccc                                                                                               <  2:1350167/89‑1 (MQ=255)
                                                                                                                   aCCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGccc                                                                                               >  1:1350167/1‑89 (MQ=255)
                                                                                                                    cccGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGa                                 <  2:718718/150‑1 (MQ=255)
                                                                                                                     ccGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCTATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGAc                                >  1:1076526/1‑150 (MQ=255)
                                                                                                                      cGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACg                               >  2:1592331/1‑150 (MQ=255)
                                                                                                                          ccATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCATGTTCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCTCCGTGCGCTTGCGCAGCGAGGTTAACGAGCACACCGTCTACCTGATCGTCGAAGACGaatg                           <  2:1340654/150‑3 (MQ=255)
                                                                                                                           cATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAg                                                           >  1:1457898/1‑117 (MQ=255)
                                                                                                                           cATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAg                                                           <  2:1457898/117‑1 (MQ=255)
                                                                                                                             ttGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAg                        >  1:505004/1‑150 (MQ=255)
                                                                                                                                ggCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGa                                                                     >  2:700958/1‑102 (MQ=255)
                                                                                                                                ggCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGa                                                                     <  1:700958/102‑1 (MQ=255)
                                                                                                                                ggCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCgg                     <  2:1746680/150‑1 (MQ=255)
                                                                                                                                    gAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAgg                           <  1:1788933/140‑1 (MQ=255)
                                                                                                                                    gAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAgg                           >  2:1788933/1‑140 (MQ=255)
                                                                                                                                         tGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTTCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGATTCCgaa            <  1:950544/150‑1 (MQ=255)
                                                                                                                                         tGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGATTCCgaa            >  1:410561/1‑150 (MQ=255)
                                                                                                                                         tGACCCGCAGCGCATGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGGTGCCTCGCCGCCCCGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGATTCCgaa            <  2:1035086/150‑1 (MQ=255)
                                                                                                                                          gACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGATTCCgaag           >  1:61488/1‑150 (MQ=255)
                                                                                                                                             ccGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAg                                                                    >  2:1259832/1‑90 (MQ=255)
                                                                                                                                             ccGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAg                                                                    <  1:1259832/90‑1 (MQ=255)
                                                                                                                                             ccGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGATTCCGAAGAAc        >  1:366735/1‑150 (MQ=255)
                                                                                                                                             ccGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGATTCCGAAGAAc        >  1:1796062/1‑150 (MQ=255)
                                                                                                                                             ccGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGGTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGATTCCGAAGAAc        >  2:357639/1‑150 (MQ=255)
                                                                                                                                              cGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGATTCCGAAGAAca       >  2:352386/1‑150 (MQ=255)
                                                                                                                                               gCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGATTCCGAAGAAcat      >  2:1006239/1‑150 (MQ=255)
                                                                                                                                                cAGCACCTGGGCCAGGCGCTGATCAACCTGCTCTCCAACGCCCGCGATGc                                                                                                         <  1:843410/50‑1 (MQ=25)
                                                                                                                                                cAGCACCTGGGCCAGGCGCTGATCAACCTGCTCTCCAACGCCCGCGATGc                                                                                                         >  2:843410/1‑50 (MQ=25)
                                                                                                                                                 aGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGATTCCGAAGAAcatca    <  2:1642718/150‑1 (MQ=255)
                                                                                                                                                  gcgcCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGATTCCGAAGAAcatcaa   <  1:1006239/150‑2 (MQ=255)
                                                                                                                                                   cgcCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGATTCCGAAGAACATCATg  <  2:480413/150‑1 (MQ=255)
                                                                                                                                                   cgcCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGATTCCGAAGAACATCATg  <  1:1592331/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGCAGCCACCAGAACAGCGAGGAGCCCGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGATTCCGAAGAACATCATG  >  NC_002947/5336079‑5336375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑