| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,453,160 | T→C | 16.7% | intergenic (+125/‑230) | lnt → / → PP_4791 | apolipoprotein N‑acyltransferase/transposase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,453,160 | 0 | T | C | 16.7% | 59.3 / 6.7 | 30 | intergenic (+125/‑230) | lnt/PP_4791 | apolipoprotein N‑acyltransferase/transposase |
| Reads supporting (aligned to +/- strand): ref base T (13/12); new base C (2/3); total (15/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01 | |||||||||||
GGGCGTATTGCAAAAACCGTCTGATTGACGATACGCGACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGA‑AAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTGCTTTGTTAGGGTTCGTGTGCGCGGGACAGAGATCGTAGCCAGCACTGGTGTGTGTCTGCACACCCGCGTAGGAGAGTGATCC > NC_002947/5453012‑5453269 | gggCGTATTGCAAAAACCGTCTGATTGACGATACGCGACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTGCGCAGCGACGCTGACATTTTGA‑AAAAGAAAAGGCGGtt > 1:99457/1‑150 (MQ=255)gggCGTATTGCAAAAACCGTCTGATTGACGATACGCGACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGA‑AAAAGAAAAGGCGGtt < 2:1403649/150‑1 (MQ=255) cGTATTGCAAAAACCGTCTGATTGACGATACGCGACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGTAAACATGAGGTGTTCGGCTTCAGTTCCGCAGCGACGCTGACATTTTGA‑AAAAGAAAAGTCGGTTGGc > 2:1038815/1‑150 (MQ=255) cAAAAACCGTCTGATTGACGATACGCGACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGA‑AAAAGAAAAGGCGGTTGGCCCGGTAg > 2:1415640/1‑150 (MQ=255) aaaaCCGTCTGATTGACGATACGCGACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGA‑AAAAGAAAAGGCGGTTGGCCCGGTAggg > 2:951924/1‑150 (MQ=255) aaaaCCGTCGGATTGACGATACGCGACCACTGTAGGAGATCACCCATCCTTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGA‑AAAAGAAAAGGCGGTTGGCCCGGTAggg < 1:681967/150‑1 (MQ=255) aCCGTCTGATTGACGATACGCGACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGA‑AAAAGAAAAGGCGGTTGGCCCGGTAGGGCCa < 1:1045601/150‑1 (MQ=255) aCCGTCTGATTGACGATACGCGACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGA‑AAAAGAAAAGGCGGTTGGCCCGGTAGGGCCa < 2:500018/150‑1 (MQ=255) tCTGATTGACGATACGCGACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGA‑AAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAgccg > 2:77375/1‑150 (MQ=255) aTTGACGATACGCGACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCCGACATTTTGA‑AAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCtt > 2:1619355/1‑150 (MQ=255) cGATACGCGACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGA‑AAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTGCt < 1:1415640/150‑1 (MQ=40) gATACGCGACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGA‑AAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAgcc > 1:57792/1‑139 (MQ=40) gATACGCGACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGA‑AAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAgcc < 2:57792/139‑1 (MQ=40) gATACGCGACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGA‑AAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTGCtt > 2:1278020/1‑150 (MQ=39) aTACGCGACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGA‑AAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTGCttt < 2:191357/150‑1 (MQ=38) aTACGCGACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTCGATCGCAGGGTA‑GCGGTCTGCCCTGGAGGGCCAGCCGCCTATTGCttt > 2:1453120/1‑150 (MQ=11) gcgACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGA‑AAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTGCTTTGTTa < 1:1268120/150‑1 (MQ=35) gcgACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGA‑AAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTGCTTTGTTa < 2:1818259/150‑1 (MQ=35) cACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGA‑AAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTGCTTTGTTAGGGtt > 2:754226/1‑150 (MQ=31) ccgtaGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGA‑AAAAGAAAAGGCGGCTGGCCCTACCGGGCCAACCGCCTTTTctttttcaa < 2:995266‑M1/141‑10 (MQ=255) ccgtaGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGA‑AAAAGAAAAGGCGGCTGGCCCTACCGGGCCAACCGCCTTTTctttttcaa > 1:995266‑M1/3‑134 (MQ=255) ggtAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGA‑AAAAGAAAAGGCGGCTGGCCCTACCGGGCCAACCGCCTTTTctttttcaaaatgtcag < 2:203587‑M1/149‑18 (MQ=255) tGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGA‑AAAAGAAAAGGCGGCTGGCCCTACCGGGCCAACCGCCTTTTc < 2:712050‑M1/106‑2 (MQ=255) tGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGA‑AAAAGAAAAGGCGGCTGGCCCTACCGGGCCAACCGCCTTTTc > 1:712050‑M1/1‑105 (MQ=255) ggCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgcagcccggaa < 2:1588062‑M1/109‑78 (MQ=255) ggCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgcagcccggaa > 1:1588062‑M1/1‑32 (MQ=255) gCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgct > 2:650958‑M1/1‑31 (MQ=255) gCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgct < 1:650958‑M1/87‑57 (MQ=255) ggTTGGCCCGGTAGGGCCAACCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgcagcccggaagggctg < 2:102450‑M1/112‑84 (MQ=255) ggTTGGCCCGGTAGGGCCAACCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgcagcccggaagggctg > 1:102450‑M1/1‑29 (MQ=255) gTTGGCCCGGTAGGGCCAGCCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgcagcccggaagg < 2:1433341‑M1/107‑80 (MQ=255) gTTGGCCCGGTAGGGCCAGCCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgcagcccggaagg > 1:1433341‑M1/1‑28 (MQ=255) | GGGCGTATTGCAAAAACCGTCTGATTGACGATACGCGACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGA‑AAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTGCTTTGTTAGGGTTCGTGTGCGCGGGACAGAGATCGTAGCCAGCACTGGTGTGTGTCTGCACACCCGCGTAGGAGAGTGATCC > NC_002947/5453012‑5453269 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |