| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,618,763 | T→A | 6.5% | intergenic (‑61/‑40) | wzy ← / → PP_4937 | O‑antigen polymerase/toluene tolerance protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,618,763 | 0 | T | A | 6.5% | 177.9 / 4.1 | 62 | intergenic (‑61/‑40) | wzy/PP_4937 | O‑antigen polymerase/toluene tolerance protein |
| Reads supporting (aligned to +/- strand): ref base T (30/28); new base A (2/2); total (32/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.75e-01 | |||||||||||
TTGCTGGGTGCCAGGGCAATCGCCGCCAGGAACCAGACCAGGCCCAAACCTAACCACGCTTGTGCCCAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACTTCGATCAGCTGACACTCGGCGCACAGGTGCTTGAGGCAGACAGCCATGGCGCCAAAGTCTACCTGCTCACA > NC_002947/5618617‑5618904 | ttgCTGGGTGCCAGGGCAATCGCCGCCAGGAACCAGACCAGGCCCAAACCTAACCACGCTTGTGCCCAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGc < 1:299137/150‑1 (MQ=255) tGGGTGCCAGGGCAATCGCCGCCAGGAACCAGACCAGGCCCAAACCTAACCACGCTTGTGCCCAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGt > 1:1509133/1‑150 (MQ=255) ccAGGGCAATCGCCGCCAGGAACCAGACCAGGCCCAAACCTAACCACGCTTGTGCCCAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCAtttt > 1:525482/1‑150 (MQ=255) ccAGGGCAATCGCCGCCAGGAACCAGACCAGGCCCAAACCTAACCACGCTTGTGCCCAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCAtttt > 1:289689/1‑150 (MQ=255) cAGGGCAATCGCCGCCAGGAACCAGACCAGGCCCAAACCTAACCACGCTTGTGCCCAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTAATGCCGGTCATTTTg > 1:606936/1‑150 (MQ=255) cAGGGCAATCGCCGCCAGGAACCAGACCAGGCCCAAACCTAACCACGCTTGTGCCCAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTAATGCCGGTCATTTTg > 1:606248/1‑150 (MQ=255) aGGGCAATCGCCGCCAGGAACCAGACCAGGCCCAAACCTAACCACGCTTGTGCCCAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGc > 1:1041687/1‑150 (MQ=255) ggCAATCGCCGCCAGGAACCAGACCAGGCCCAAACCTAACCACGCTTGTGCCCAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTc < 2:1698203/150‑1 (MQ=255) ggCAATCGCCGCCAGGAACCAGACCAGGCCCAAACCTAACCACGCTTGTGCCCAGTTTTTTTGATACAACATCCTTTCCCCCGATCAATCAGAAACGCCATCCATGGTGCTACAGC‑CCATTTAAGGTCATTATTGCCGGTCATTTTGCTc < 1:1483594/150‑1 (MQ=255) cAATCGCCGCCAGGAACCAGACCAGGCCCAAACCTAACCACGCTTGTGCCCAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGc < 2:1098864/150‑1 (MQ=255) aaTCGCCGCCAGGAACCAGACCAGGCCCAAACCTAACCACGCTTGTGCCCAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGcc > 1:1397723/1‑150 (MQ=255) tCGCCGCCAGGAACCAGACCAGGCCCAAACCTAACCACGCTTGTGCCCAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAg > 2:525391/1‑150 (MQ=255) aGGAACCAGACCAGGCCCAAACCTAACCACGCTTGTGCCCAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCAttt < 1:173840/132‑1 (MQ=255) aGGAACCAGACCAGGCCCAAACCTAACCACGCTTGTGCCCAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCAttt > 2:173840/1‑132 (MQ=255) ggAACCAGACCAGGCCCAAACCTAACCACGCTTGTGCCCAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAgg < 2:1449306/150‑1 (MQ=255) ccagaccagGCCCAAACCTAACCACGCTTGTGCCCAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGctc < 2:1024167/149‑1 (MQ=255) ccagaccagGCCCAAACCTAACCACGCTTGTGCCCAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGctc > 1:1024167/1‑149 (MQ=255) cagaccagGCCCAAACCTAACCACGCTTGTGCCCAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTa > 1:396580/1‑150 (MQ=255) ggCCCAAACCTAACCACGCTTGTGCCCAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTAATGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGc < 2:606248/150‑1 (MQ=255) ggCCCAAACCTAACCACGCTTGTGCCCAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTAATGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGc < 2:606936/150‑1 (MQ=255) aCCTAACCACGCTTGTGCCCAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTc > 1:1601605/1‑150 (MQ=255) ccgaaCCACGCTTGTGCCCAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTcc < 1:379402/147‑1 (MQ=255) ccTAACCACGCTTGTGCCCAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCCTTTTGCTCGCCAGACGACAAGGCTCTCTTCATGCCATgtcct < 2:1509133/150‑5 (MQ=255) cTAACCACGCTTGTGCCCAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTccc < 2:656368/150‑1 (MQ=255) aaCCACGCTTGTGCCCAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCgg > 1:777215/1‑150 (MQ=255) cTTGTGCCCAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATACTCCCGGCTCAccc > 1:728063/1‑150 (MQ=255) tgCCCAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGt < 2:123732/150‑1 (MQ=255) cccAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCg > 1:1675996/1‑150 (MQ=255) cAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCTGGCTCACACAGGTCGAc > 2:1386072/1‑150 (MQ=255) gTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACtt < 2:1914401/150‑1 (MQ=255) ttGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACTTCGATCa < 2:1217796/150‑1 (MQ=255) tGATACAACATCCTTTCCCCCGATGAATCAGAAACACCCTCCCTGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACTTCGATCAg > 2:563183/1‑150 (MQ=255) tACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACTTCGAt > 1:656304/1‑144 (MQ=255) tACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACTTCGAt < 2:656304/144‑1 (MQ=255) acaacaTCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACTTCGATCAGCTGa < 2:936724/150‑1 (MQ=255) aacaTCCTTTCCCCCGATGAATCAGAAACACAATACATGGTGCTACACCCTTATTTACAG‑CACAATGGGCGCGAGGATTCCTCGCCTGACGACAATGCTGTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACTTCGATCAGCTGaca > 2:215786/1‑150 (MQ=255) aTCCTTTCTCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACTTCGATCAGCTGACACTc < 2:1041687/150‑1 (MQ=255) cTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACTTCGATCAGCTGACACTCGgc < 1:1246509/150‑1 (MQ=255) tttCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGct < 2:180731/92‑1 (MQ=255) tttCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGct > 1:180731/1‑92 (MQ=255) tttCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACTTCGATCAGCTGACACTCGgcg > 1:1522126/1‑150 (MQ=255) cccGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACTTCGATCAGCTGACACCCGGCGcaccg > 1:266610/1‑148 (MQ=255) aTGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACTTCGa < 1:556726/124‑1 (MQ=255) aTGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACTTCGa > 2:556726/1‑124 (MQ=255) gactCAGCACCACCCGCCATGGTGCTACAGCATTATTTTCGG‑CATTCTTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCACTCCGGCAAACTCACCCAGGTCGACTTCGATCAGCTGACTCTCGGCGCACAGTTGCtt < 2:266610/147‑1 (MQ=255) tCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACTTCGATCAGCTGACACTCGGCGCACAGGTGCTTGAg > 1:681096/1‑150 (MQ=255) tCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACTTCGATCAGCTGACACTCGGCGCACAGGTGATTGAg < 2:777215/150‑1 (MQ=255) cAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGATCCCGGCTCAGCCCGGTCGACTTCGATCAGCTGACTCTCCGCGCATAGGTGCTTGAgg > 1:728824/1‑150 (MQ=255) aaaCACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACTTCGATCAGCTGACACTCGGCGCACAGGTGCTTGAGGCAg > 2:700371/1‑150 (MQ=255) aaaCACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACTTCGATCAGCTGACACTCGGCGCACAGGTGCTTGAGGCAg > 2:1509790/1‑150 (MQ=255) catccatGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACTTCGATCAGCTGACACTCGGCGCACAGGTGCTTGAGGCAGACAGcc > 2:1871372/1‑150 (MQ=255) tccatGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACTTCGATCAGCTGACACTCGGCGCACAGGTGCTTGa > 2:1642846/1‑137 (MQ=255) tccatGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACTTCGATCAGCTGACACTCGGCGCACAGGTGCTTGa < 1:1642846/137‑1 (MQ=255) cctGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACTTCGATCAGCTGACACTCGGCGCACAGGTGCTTGAGGCAGACAGCCATgg < 1:91201/148‑1 (MQ=255) cTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACTTCGATCAGCTGACACTCGGCGCACAGGTGCTTGAGGCAGACAGCCATGGCGCCaaa < 2:289689/150‑1 (MQ=255) cTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACTTCGATCAGCTGACACTCGGCGCACAGGTGCTTGAGGCAGACAGCCATGGCGCCaaa < 2:184758/150‑1 (MQ=255) aCAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACTTCGCTCAGCTGACACCCGCCCCTCATGTGCTTGAGGCAGACAGCCATGGCGCCAAAgc > 2:682808/1‑149 (MQ=255) aGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCGGGCTCACCCAGGTCGACTTCGATCAGCTGACACTCGGCGCACAGGTGCTTGAGGCAGACAGCCATGGCGCCAAAGTCt > 2:295977/1‑150 (MQ=255) cATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACTTCGATCAGCTGACACTCGGCGCACAGGTGCTTGAGGCAGACAGCCATGGCGCCAAAGTCTAc < 2:1675996/150‑1 (MQ=255) cGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACTTCGATCAGCTGACACTCGGCGCACAGGt < 2:52991/107‑1 (MQ=255) cGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACTTCGATCAGCTGACACTCGGCGCACAGGt > 1:52991/1‑107 (MQ=255) cGG‑CATTATTGCCGGTCATTTTGCGCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACTTCGATCAGCTGACACTCGGCGCACAGGTGCTTGAGGCAGACAGCCATGGCGCCAAAGCCTACCTGCTcaca < 1:682808/150‑1 (MQ=255) | TTGCTGGGTGCCAGGGCAATCGCCGCCAGGAACCAGACCAGGCCCAAACCTAACCACGCTTGTGCCCAGTTTTTTTGATACAACATCCTTTCCCCCGATGAATCAGAAACACCATCCATGGTGCTACAGCATTATTTTCGG‑CATTATTGCCGGTCATTTTGCTCGCCAGACGACAAGGCTCTATTCATGCAATGCTCCCGGCTCACCCAGGTCGACTTCGATCAGCTGACACTCGGCGCACAGGTGCTTGAGGCAGACAGCCATGGCGCCAAAGTCTACCTGCTCACA > NC_002947/5618617‑5618904 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |