BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,732,025	A→T	5.8%	L196* (TTG→TAG) ‡	hutI ←	imidazolonepropionase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,732,025	0	A	T	5.8%	314.5 / 9.1	105	L196* (TTG→TAG) ‡	hutI	imidazolonepropionase
Reads supporting (aligned to +/- strand): ref base A (47/50); new base T (3/3); total (51/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.19e-01

Only 100 of 106 total aligned reads displayed.

CGTGCAACGATGACAGTTGTTCGGCATGCAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGCTCGTCGGCCAGGCGGCGGGCCACTCGCAGCATCTTG  >  NC_002947/5731879‑5732174

cGTGCAACGATGACAGTTGTTCGGCATGCAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGAGCCGGCGAGAACGTGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCGTCGC‑CGGCCAAgg                                                                                                                                                     >  2:1030507/1‑150 (MQ=255)

    cAACGATGACAGTTGTTCGGCATGCAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCgg                                                                                                                                                 >  2:1398102/1‑150 (MQ=255)

     aaCGATGACAGTTGTTCGGCATGCAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTTGC‑CGGCCTTGGCCGGc                                                                                                                                                >  1:1703833/1‑150 (MQ=255)

     aaCGATGACAGTTGTTCGGCATGCAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGc                                                                                                                                                >  2:1370290/1‑150 (MQ=255)

     aaCGATGACAGTTGTTCGGCATGCAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGc                                                                                                                                                <  1:1538867/150‑1 (MQ=255)

       cGATTACAGTTGTTCGGCAGGCAGATTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGGACAGCCGCTCGACCTGGGCCGGCGATAACGCGAGGTGTCCGCAGAAGCCAGCCACCGCCCCCAGCAGGCCTTCGC‑AGGCCAAGGCCGGCaa                                                                                                                                              <  2:340697/150‑1 (MQ=255)

        gaggtgaGTTGTTCGGCATGCAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAAc                                                                                                                                             <  2:400135/144‑1 (MQ=255)

        gATGACAGTTGTTCGGCATGCAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAAc                                                                                                                                             >  2:164866/1‑150 (MQ=255)

         aTGACAGTTGTTCGGCATGCAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTTGC‑CGGCCTTGGCCGGcgaag                                                                                                                                            <  2:1703833/150‑5 (MQ=255)

         aTGACAGTTGTTCGGCATGCAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGATCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCTTGGCCGGcgaag                                                                                                                                            <  2:1022636/150‑5 (MQ=255)

             cAGTTGTTCGGCATGCAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGGCCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACAtctc                                                                                                                                        <  1:505800/150‑1 (MQ=255)

              aGTTGTTCGGCATGCAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCg                                                                                                                                       >  2:121772/1‑150 (MQ=255)

               gttgttCGGCATGCAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGt                                                                                                                                      <  1:189352/150‑1 (MQ=255)

                ttgttCGGCATGCAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTc                                                                                                                                     >  1:558476/1‑150 (MQ=255)

                 tgttCGGCATGCAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTca                                                                                                                                    >  1:767717/1‑150 (MQ=255)

                   ttCGGCATGCAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTcaca                                                                                                                                  <  2:827037/150‑1 (MQ=255)

                   ttCGGCATGCAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGGcaca                                                                                                                                  >  1:585796/1‑150 (MQ=255)

                    tCGGCATGCAGCTTGCCCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAg                                                                                                                                 <  1:189971/150‑1 (MQ=255)

                      ggCATGCAGCTTGACGGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACATAGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGAt                                                                                                                               <  2:357176/150‑1 (MQ=255)

                      ggCATGCAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGAt                                                                                                                               <  1:392234/150‑1 (MQ=255)

                        cATGCAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGAtgt                                                                                                                             <  2:657935/150‑1 (MQ=255)

                         aTGCAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCg                                                                                                                                                  <  2:1049831/128‑1 (MQ=255)

                         aTGCAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCg                                                                                                                                                  >  1:1049831/1‑128 (MQ=255)

                          tGCAGCTTGACCGGCAGGCCCAGTGCGCGCGGCTTGAGGGACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGTCAAGGCCGGCAACATCTCGTCACAGTTGTgc                                                                                                                           <  2:391701/150‑1 (MQ=255)

                            cAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTgcgc                                                                                                                         <  1:197076/150‑1 (MQ=255)

                              gCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGACCAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGa                                                                                                                       <  2:1399998/150‑1 (MQ=255)

                              gCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGa                                                                                                                       <  1:598397/150‑1 (MQ=255)

                               cTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCTGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGAt                                                                                                                      >  2:1314790/1‑150 (MQ=255)

                                  gACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTa                                                                                                                   <  2:1331468/150‑1 (MQ=255)

                                  gACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTa                                                                                                                   <  2:558476/150‑1 (MQ=255)

                                   aCCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAg                                                                                                                  >  2:1634707/1‑150 (MQ=255)

                                         aGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGtcgtcg                                                                                                            <  2:939733/150‑1 (MQ=255)

                                           gCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCTGTCCTTCGC‑CGGCCAAGGCCg                                                                                                                                                  >  1:27021/1‑110 (MQ=255)

                                           gCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCg                                                                                                                                                  <  2:27021/110‑1 (MQ=255)

                                            cccAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGc‑c                                                                                                        >  1:905517/1‑150 (MQ=255)

                                             ccAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGc‑cc                                                                                                       >  1:214674/1‑150 (MQ=255)

                                             ccAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGc‑cc                                                                                                       >  2:299404/1‑150 (MQ=255)

                                              cAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCGACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCg                                                                                                      >  1:632916/1‑150 (MQ=255)

                                                 ttCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGCTGTGCGCGACGTAGGGGGCTGC‑CCGAcc                                                                                                   >  2:901318/1‑150 (MQ=255)

                                                  tCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGAccc                                                                                                  <  1:1244286/150‑1 (MQ=255)

                                                   cgcgcgCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCcg                                                                                                 <  1:299404/150‑1 (MQ=255)

                                                     cgcgcCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCcgcg                                                                                               <  2:1215488/150‑1 (MQ=255)

                                                      gcgcCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATACACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGt                                                                                              >  2:65046/1‑150 (MQ=255)

                                                          cTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTc                                                                                          >  2:955463/1‑150 (MQ=255)

                                                            tggtgaACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCg                                                                                        >  1:1221825/4‑150 (MQ=255)

                                                             gatgaACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCgg                                                                                       >  2:1761762/1‑150 (MQ=255)

                                                                gaACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGTTCCGCGTACTCCggggg                                                                                    <  1:1499218/150‑1 (MQ=255)

                                                                  aCAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCa                                                                                  >  1:439266/1‑150 (MQ=255)

                                                                    aGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCaaa                                                                                <  1:1350468/150‑1 (MQ=255)

                                                                    aGCCGCTCGACCTGGGCCGGCGAGAAAACTAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCaaa                                                                                <  1:1435894/150‑1 (MQ=255)

                                                                     gCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAg                                                                               >  1:436146/1‑150 (MQ=255)

                                                                     gCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGGTCGCAGAAGGCATCCACCGCATCCACCAGGCCGTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAg                                                                               <  2:130823/150‑1 (MQ=255)

                                                                        gCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACAtctc                                                                                                                                        <  2:45894/91‑1 (MQ=255)

                                                                        gCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACAtctc                                                                                                                                        >  1:45894/1‑91 (MQ=255)

                                                                        gCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGc‑cc                                                                                                       <  1:748712/123‑1 (MQ=255)

                                                                        gCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGc‑cc                                                                                                       >  2:748712/1‑123 (MQ=255)

                                                                           cGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTAATCCGGGGGCAAAGCGTgcg                                                                         <  1:65046/150‑1 (MQ=255)

                                                                                  ggCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCaaa                                                                                >  1:401750/1‑136 (MQ=255)

                                                                                      ggCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTc                                                                                                                                     >  2:1624502/1‑80 (MQ=255)

                                                                                         gagaACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTg                                                           <  1:121772/150‑1 (MQ=255)

                                                                                         gagaACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGAGGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTg                                                           >  2:800460/1‑150 (MQ=255)

                                                                                               gcgAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCggg                                                                                      <  1:1107879/117‑1 (MQ=255)

                                                                                               gcgAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCggg                                                                                      >  2:1107879/1‑117 (MQ=255)

                                                                                               gcgAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGgcacgc                                                     >  2:804668/1‑150 (MQ=255)

                                                                                               gcgAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGgcacgc                                                     >  2:192720/1‑150 (MQ=255)

                                                                                                 gAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGgcacgcac                                                   >  1:1285830/1‑150 (MQ=255)

                                                                                                    gtgtTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGt                                                <  2:767717/150‑1 (MQ=255)

                                                                                                       ttCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCa                                                                   <  1:1693551/128‑1 (MQ=255)

                                                                                                       ttCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCa                                                                   >  2:1693551/1‑128 (MQ=255)

                                                                                                       ttCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑AGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAg                                             >  1:1404496/1‑150 (MQ=255)

                                                                                                          gCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCgg                                          <  1:804668/150‑1 (MQ=255)

                                                                                                           cAGAAGGCATCCACCGCATCCACCAGGCCTTCGT‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGc                                         <  2:984160/150‑1 (MQ=255)

                                                                                                           cAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGc                                         <  1:1761762/150‑1 (MQ=255)

                                                                                                             gAAGGCATCCACCGCATCCACCAGGCCTTCGCACGAACCA‑GCAAGAAACCCCGTGGCTCAGATGGGCGCGGTGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAg                                       >  1:976465/1‑150 (MQ=255)

                                                                                                             gAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAg                                       <  2:1221825/150‑1 (MQ=255)

                                                                                                                  catccaccgcatccaccAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGCtcgt                                  >  1:500348/1‑150 (MQ=255)

                                                                                                                   atccaccgcatccaccAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCCCCGTCGGCGGCAGCtcgtc                                 >  2:110341/1‑150 (MQ=255)

                                                                                                                    tccaccgcatccaccAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGCtcgtcg                                >  2:1137013/1‑150 (MQ=255)

                                                                                                                    tccaccgcatccaccAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGCtcgtcg                                >  1:489070/1‑150 (MQ=255)

                                                                                                                                accaGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGCTGTAGTCGGCGGC‑CCGTCCCGCGTACTCCGGGGTCAAAGCGTGCGCCGCCATGCACGTGGCACGCACCGTCAGCGGCAGCTCGTCGGCCAGGcggcgg                    <  2:214674/150‑1 (MQ=255)

                                                                                                                                accaGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGCTCGTCGGCCAGGcggcgg                    <  1:1314790/150‑1 (MQ=255)

                                                                                                                                accaGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGCTCGTCGGCCAGGcggcgg                    >  1:500991/1‑150 (MQ=255)

                                                                                                                                accaGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGCTCGTCGGCCAGGcggcgg                    <  2:1913860/150‑1 (MQ=255)

                                                                                                                                 ccaGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGCTCGTCGGCCAGGCGGCggg                   <  1:1030507/150‑1 (MQ=255)

                                                                                                                                 ccaGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGCTCGTCGGCCAGGCGGCggg                   >  1:1853739/1‑150 (MQ=255)

                                                                                                                                 ccaGGCCTTCGC‑CGGCCAAGGCCGATAGGCGCGCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGCTCGTCGGCCAGGCGGCggg                   >  1:528180/1‑150 (MQ=255)

                                                                                                                                    ggCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGCTCGTCGGCCAGGCGGCGGGccc                >  2:1297580/1‑149 (MQ=255)

                                                                                                                                     gCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGCTCGTCGGCCAGGCGGCGGGCCAc               <  2:489070/150‑1 (MQ=255)

                                                                                                                                       cTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCa                                        >  1:74865/1‑123 (MQ=255)

                                                                                                                                       cTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCa                                        <  2:74865/123‑1 (MQ=255)

                                                                                                                                       cTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGCTCGTCGGCCAGGCGGCGGGCCACTc             >  1:945680/1‑150 (MQ=255)

                                                                                                                                       cTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGCTCGTCGGCCAGGCGGCGGGCCACTc             >  1:343659/1‑150 (MQ=255)

                                                                                                                                       cTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGCTCGTCGGCCAGGCGGCGGGCCACTc             <  2:343659/150‑1 (MQ=255)

                                                                                                                                        ttCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGCTCGTCGGCCAGGCGGCGGGCCACTCg            <  1:1137013/150‑1 (MQ=255)

                                                                                                                                         ttgC‑CGGCCTTGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTgc                                                                          <  1:1597319/85‑1 (MQ=255)

                                                                                                                                         ttgC‑CGGCCTTGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTgc                                                                          >  2:1597319/3‑87 (MQ=255)

                                                                                                                                         ttgC‑CGGCCTTGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGCTGGACGGCGAGGCGGGGGGCCACTCgc           >  1:1662767/3‑150 (MQ=255)

                                                                                                                                            c‑cGGCCCAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGCTCGTCGGCCAGGCGGCGGGCCACTCgcagc        <  1:1022066/150‑1 (MQ=255)

                                                                                                                                                gCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGCTCGTCGGCCAGGCGGCGGGCCACTCGCAGCATc     >  1:1616064/1‑150 (MQ=255)

                                                                                                                                                   aaGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGCCGTAGGTAGCGA‑TCGTTTTCTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGCTCGTCGGCCAGGCGGCGGGCCACTCGCAGCATCTTg  <  2:528180/150‑1 (MQ=255)

CGTGCAACGATGACAGTTGTTCGGCATGCAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGC‑CGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGC‑CCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGCTCGTCGGCCAGGCGGCGGGCCACTCGCAGCATCTTG  >  NC_002947/5731879‑5732174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`