Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,816,909 A→G 12.1% intergenic (+39/+90) PP_5092 → / ← pilT NLP/P60 protein/twitching motility protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,816,9090AG12.1% 210.4 / 15.6 83intergenic (+39/+90)PP_5092/pilTNLP/P60 protein/twitching motility protein
Reads supporting (aligned to +/- strand):  ref base A (37/35);  new base G (4/6);  total (42/41)
Fisher's exact test for biased strand distribution p-value = 7.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

AAGCGGGTGAGTATCGACACCTTGAGCAAGCCTTACTGGCAGAAGCACTACGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAGGGCCTTTGCAGGTACTACAAGCCTTTGATCAACCCTCGCTCCCTCAGCCTCCGCAACGCCCCCTCCATCGTC  >  NC_002947/5816763‑5817056
                                                                                                                                                  |                                                                                                                                                   
aaGCGGGTGAGTATCGACACCTTGAGCAAGCCTTACTGGCAGAAGCACTACGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGC                                                                                                                                                  >  2:748376‑M1/1‑150 (MQ=255)
     ggTGAGTATCGACACCTTGAGCAAGCCTTACTGGCAGAAGCACTACGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCT                                                                                                                                             >  1:44540‑M1/1‑150 (MQ=255)
      gTGAGTATCGACACCTTGAGCAAGCCTTACTGGCAGAAGCACTACGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTC                                                                                                                                            >  2:1174112‑M1/1‑150 (MQ=255)
       tGAGTATCGACACCTTGAGCAAGCCTTACTGGCAGAAGCACTACGTGGTGGCCAAGCGGGTGCTGCCGCAAATACGGCAGCTGAGTCTTGCCATGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCGTGCCCGCgaa                                                                                                                                           <  1:231420‑M1/150‑4 (MQ=255)
       tGAGTATCGACACCTTGAGCAAGCCTTACTGGCAGAAGCACTACGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCGTGCCCGCgaa                                                                                                                                           <  2:147612‑M1/150‑4 (MQ=255)
         gcggttggcgcagttgtcttcagtgatgcagggcggggcggcggaggggatgctgacgatggagggggcgttgcggaggctgagggagcgagggttgatcaaaggcttgtagtacctgcaaaGGCCCCTTCGCGGGCACGCCCGCTCCca                                                                                                                                         >  1:471545‑M1/123‑150 (MQ=255)
         aGTATCGACACCTTGAGCAAGCCTTACTGGCAGAAGCACTACGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCGTGCCCGCgaagg                                                                                                                                         <  1:1665034‑M1/150‑6 (MQ=255)
         aGTATCGACACCTTGAGCAAGCCTTACTGGCAGAAGCACTACGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCA                                                                                                                                         <  1:1107064‑M1/150‑1 (MQ=255)
            aTCGACACCTTGAGCAAGCCTTACTGGCAGAAGCACTACGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAG                                                                                                                                      <  1:217673‑M1/150‑1 (MQ=255)
               ggcgcagttgtcttcagtgatgcagggcggggcggcggaggggatgctgacgatggagggggcgttgcggaggctgagggagcgagggttgatcaaaggcttgtagtacctgcaaaGGCCCTTTCGCGGGCACGCCCGCTCCCACAGGTa                                                                                                                                   <  2:1435932‑M1/34‑1 (MQ=255)
                acacCTTGAGCAAGCCTTACTGGCAGAAGCACTACGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAg                                                                                                                                  >  1:1052869/1‑150 (MQ=255)
                    cTTGAGCAAGCCTTACTGGCAGAGGCACTACGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCTTTCGCGGGCGCGCCCGCTCCCACAGGTAtgtga                                                                                                                              >  2:942122‑M1/1‑145 (MQ=255)
                    cTTGAGCAAGCCTTACTGGCAGAAGCACTACGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGcgcg                                                                                                                              >  1:1752754/1‑150 (MQ=255)
                    cTTGAGCAAGCCTTACTGGCAGAAGCACTACGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCA                                                                                                                                         >  1:709601‑M1/1‑139 (MQ=255)
                    cTTGAGCAAGCCTTACTGGCAGAAGCACTACGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCA                                                                                                                                         <  2:709601‑M1/139‑1 (MQ=255)
                     ttGAGCAAGCCTTACTGGCAGAAGCACTACGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAtgtgag                                                                                                                             >  1:1582974‑M1/1‑144 (MQ=255)
                     ttGAGCAAGCCTTACTGGCAGAAGCACTACGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAtgtgag                                                                                                                             >  1:1583520‑M1/1‑144 (MQ=255)
                     ttGAGCAAGCCTTACTGGCAGAAGCACTACGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGt                                                                                                                             <  2:633132/150‑1 (MQ=255)
                         gCAAGCCTTACTGGCAGAAGCACTACGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGct                                                                                                                         >  1:429430/1‑150 (MQ=255)
                          cAAGCCTTACTGGCAGAAGCACTACGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGctc                                                                                                                        <  2:1064889/150‑1 (MQ=255)
                           aaGCCTTACTGGCAGAAGCACTACGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAtgtgagctgcgc                                                                                                                       >  1:1862180‑M1/1‑138 (MQ=255)
                            aGCCTTACTGGCAGAAGCACTACGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCtctc                                                                                                                      <  1:1306192/150‑1 (MQ=255)
                              ccTTACTGGCAGAAGCACTACGTGGTGGCCATGCCGGTGCTACAAACTATAAATCAACTGTGTCTGGCCAAGCGTTAAGCTGATCGGCGCTCTCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTcgc                                                                                                                    <  2:989741/150‑1 (MQ=255)
                                ttACTGGCAGAAGCACTACGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAgcgc                                                                                                                               <  2:82100/137‑1 (MQ=255)
                                ttACTGGCAGAAGCACTACGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAgcgc                                                                                                                               >  1:82100/1‑137 (MQ=255)
                                   cTGGCAGAAGCACGACGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCa                                                                                                               <  2:853820/150‑1 (MQ=255)
                                    tGGCAGAAGCACTACGTGGTGGCCAAGCGGGTGCATCCTAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAg                                                                                                              >  1:1893789/1‑150 (MQ=255)
                                        aGAAGCACTACGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTca                                                                                                          <  1:1049127/150‑1 (MQ=255)
                                          aaGCACTACGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTcaca                                                                                                        <  2:429430/150‑1 (MQ=255)
                                            gCACTACGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACAta                                                                                                      >  2:1768045‑M2/1‑148 (MQ=255)
                                               cggcggaggggatgctgacgatggagggggcgttgcggaggctgagggagcgagggttgatcaaaggcttgtagtacctgcaaaGGCCCTTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCt                                                                                                   >  1:1435932‑M1/85‑150 (MQ=255)
                                                tACGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACAtacctg                                                                                                  <  1:80579‑M2/150‑7 (MQ=255)
                                                 gcggaggggatgctgacgatggagggggcgttgcggaggctgagggagcgagggttgatcaaaggcttgtagtacctgcaaaGGCCCTTTCGCGGGCGCGCCCGCTCCCACAGGTAGAGCGTCGCTCTCGGGCGCAGATCACATACAtgt                                                                                                 >  2:551239‑M1/83‑150 (MQ=255)
                                                 aCGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACAtacctgt                                                                                                 >  2:80579‑M2/1‑143 (MQ=255)
                                                  cGTGGTGGCCACGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACAtacctgtg                                                                                                >  1:855155‑M2/1‑142 (MQ=255)
                                                  cGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCTTTCGCGGGCGCGCCCGCTCCCACAGGTAtgtgagctgcgcgcgagagcgacgcgctacctgtg                                                                                                <  1:942122‑M1/150‑36 (MQ=255)
                                                  cGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAtgtgagctgcgcgcgagagcgacgcgctacctgtg                                                                                                <  2:1583520‑M1/150‑36 (MQ=255)
                                                  cGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAtgtgagctgcgcgcgagagcgacgcgctacctgtg                                                                                                <  2:1582974‑M1/150‑36 (MQ=255)
                                                  cGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACAtacctgtg                                                                                                >  1:1174112‑M2/1‑142 (MQ=255)
                                                   gtggtgGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCCCGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACAtacctgtgg                                                                                               >  1:985635‑M2/1‑141 (MQ=255)
                                                   gtggtgGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAtgtgagctgcgcgcgagagcgacgcgctacctgtgg                                                                                               <  2:1039869‑M1/150‑37 (MQ=255)
                                                   gtggtgGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACAtacctgtgg                                                                                               >  1:748376‑M2/1‑141 (MQ=255)
                                                   gtggtgGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACAtacctgtgg                                                                                               >  2:277059‑M2/1‑141 (MQ=255)
                                                     ggtggGCAAACAGGTGCTGGCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCTTTCGCGGGCGCGCCCGCTCCCACAGGTAtgtgagctgcgcgcgagagcgacgcgctacctgtggga                                                                                             <  2:1356520‑M1/150‑39 (MQ=255)
                                                     ggtggCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACAtacctgtggga                                                                                             >  2:1921326‑M2/1‑139 (MQ=255)
                                                      gtggCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAtgtgagctgcgcgcgagagcgacgcgctacctgtgggag                                                                                            <  2:754482‑M1/150‑40 (MQ=255)
                                                       gggatgctgacgatggagggggcgttgcggaggctgagggagcgagggttgatcaaaggcttgtagtacctgcaaaGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCtgtg                                                                                                <  1:1355462‑M1/69‑1 (MQ=255)
                                                       gggatgcggacgatggagggggcgttgcggaggctgagggagcgagggttgatcaaaggcttgtagtacctgcaaaGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCtgtg                                                                                                >  2:1355462‑M1/77‑145 (MQ=255)
                                                         gCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAtgtgagctgcgcgcgagagcgacgcgctacctgtgggagcgg                                                                                         <  2:1862180‑M1/150‑43 (MQ=255)
                                                          ccAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACAtacctgtgggagcggg                                                                                        >  1:1531035‑M2/1‑134 (MQ=255)
                                                          ccAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACAtacctgtggga                                                                                             >  2:650313‑M2/1‑134 (MQ=255)
                                                          ccAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACAtacctgtggga                                                                                             <  1:650313‑M2/145‑12 (MQ=255)
                                                           tgctgacgatggagggggcgttgcggaggctgagggagcgagggttgatcaaaggcttgtagtacctgcaaaGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTgg                                                                                               <  2:132595‑M1/70‑1 (MQ=255)
                                                           tgctgacgatggagggggcgttgcggaggctgagggagcgagggttgatcaaaggcttgtagtacctgcaaaGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTgg                                                                                               >  1:132595‑M1/73‑142 (MQ=255)
                                                              gCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACAtac                                                                                                     >  2:1309345‑M2/1‑130 (MQ=255)
                                                              gCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACAtac                                                                                                     <  1:1309345‑M2/133‑4 (MQ=255)
                                                               gacgatggggggggcgttgcggaggctgagggagcgagggttgaacgaaggcttgtagtacctgcaaaGGCCCTTTCGCGGGCGCGCCCGCTCCGACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGgcgcgc                                                                                   >  1:681888‑M1/69‑150 (MQ=255)
                                                                       gcCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCACACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGaaa                                                                           <  2:1381464/150‑1 (MQ=255)
                                                                       agggggcgttgcggaggctgagggagcgagggttgatcaaaggcttgtagtacctgcaaaGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTgg                                                                                               >  1:1326820‑M1/61‑130 (MQ=255)
                                                                       agggggcgttgcggaggctgagggagcgagggttgatcaaaggcttgtagtacctgcaaaGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTgg                                                                                               <  2:1326820‑M1/70‑1 (MQ=255)
                                                                         cGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAgg                                                                         <  2:44540/150‑1 (MQ=255)
                                                                          gAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACAtacctgtgg                                                                                               >  1:1213277‑M2/1‑118 (MQ=255)
                                                                          gAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACAtacctgtgg                                                                                               <  2:1213277‑M2/127‑10 (MQ=255)
                                                                          gAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGT                                                                                                                                    >  1:443175‑M1/1‑90 (MQ=255)
                                                                          gAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGT                                                                                                                                    <  2:443175‑M1/90‑1 (MQ=255)
                                                                               agcagcagcTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAGGGCCttt                                                                   >  1:1903194/1‑150 (MQ=255)
                                                                                   ggaggctgagggagcgagggttgatcaaaggcttgtagtacctgcaaaGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTgg                                                                                               <  1:623273‑M1/70‑1 (MQ=255)
                                                                                   ggaggctgagggagcgagggttgatcaaaggcttgtagtacctgcaaaGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTgg                                                                                               >  2:623273‑M1/49‑118 (MQ=255)
                                                                                   gcagcTGAGTCTGGCCAAGCGTTAAGCTGCTCGACGTCTTCCGGTACCGGCCCCTGCGCTGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAGGGCCTTTGCAg                                                               <  2:1893789/150‑1 (MQ=39)
                                                                                    cagcTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAGGGCCTTTGCAgg                                                              <  2:1903194/150‑1 (MQ=255)
                                                                                                gCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTc                                                                                                                            >  2:228551/1‑76 (MQ=255)
                                                                                                gCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTc                                                                                                                            <  1:228551/76‑1 (MQ=255)
                                                                                                gCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAGGGCCTTTGCAGGTACTACAAGCCt                                                  <  1:1727771/150‑1 (MQ=255)
                                                                                                 ccAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCGCGCCCGCTCCCACAGGTAtgtgagctgcgcgcgagagcgacgcgctacctgt                                                                                                 >  2:350253‑M1/1‑68 (MQ=255)
                                                                                                 ccAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCGCGCCCGCTCCCACAGGTAtgtgagctgcgcgcgagagcgacgcgctacctgt                                                                                                 <  1:350253‑M1/102‑35 (MQ=255)
                                                                                                 ccAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAGGGCCTTTGCAGGTACTACAAGCCtt                                                 >  2:1443399/1‑150 (MQ=255)
                                                                                                     gCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAGGGCCTTTGCAGGTACTACAAGCCTTTGAt                                             <  1:1443399/150‑1 (MQ=255)
                                                                                                                  cGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAtgtgagctgcgcgcgagagcgacgcgctacctgt                                                                                                 <  2:11652‑M1/85‑35 (MQ=255)
                                                                                                                  cGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAtgtgagctgcgcgcgagagcgacgcgctacctgt                                                                                                 >  1:11652‑M1/1‑51 (MQ=255)
                                                                                                                   ggCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAGGGCCTTTGCAGGTCCTACATGCCTCTCATCAACTCACGGTccc                               >  2:240522/1‑150 (MQ=37)
                                                                                                                   ggCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAGGGCCTTTGCAGGTACTACAAGCCTTTGATCAACCCTCGCTccc                               >  1:876635/1‑150 (MQ=255)
                                                                                                                                 ccGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCGCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAGGGCATTTGCAGGTACTACAAGCCTTTGATCAACCCTCGCTCCCTCAGCCTCCGCAAc                 <  1:240522/150‑1 (MQ=255)
                                                                                                                                                gCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAGGGCCTTTTCAGGTACTACAAGCCTTTGATCAACCCTCGCTCCCTCAGCCTCCGCAACGCCCCCTCCATCGTc  >  2:357620/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AAGCGGGTGAGTATCGACACCTTGAGCAAGCCTTACTGGCAGAAGCACTACGTGGTGGCCAAGCGGGTGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAGGGCCTTTGCAGGTACTACAAGCCTTTGATCAACCCTCGCTCCCTCAGCCTCCGCAACGCCCCCTCCATCGTC  >  NC_002947/5816763‑5817056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: