Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,864,241 G→T 6.9% R656S (CGC→AGC)  cadA‑III ← cadmium translocating P‑type ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,864,2410GT 6.9% 221.4 / 4.7 73R656S (CGC→AGC) cadA‑IIIcadmium translocating P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base G (32/36);  new base T (3/2);  total (35/38)
Fisher's exact test for biased strand distribution p-value = 6.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.47e-01

GATGTTCTGCATGAGGATCGCCGCACTTTGGCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTCCCACCACGGCAGCAATGGCCTGC  >  NC_002947/5864092‑5864389
                                                                                                                                                     |                                                                                                                                                    
gATGTTCTGCATGAGGATCGCCGCACTTTGGCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGcgcg                                                                                                                                                      >  1:1396733/1‑150 (MQ=255)
     tCTGCATGAGGATCGCCGCACTTTGGCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCAGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCa                                                                                                                                                 <  1:1678464/150‑1 (MQ=255)
        gCATGAGGATCGCCGCACTTTGGCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCCGCCATGGCAAAACCGATTTCGGCGCGGGCCAgcg                                                                                                                                              >  1:757579/1‑150 (MQ=255)
         cATGAGGATCGCCGCACTTTGGCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGCGCCAgcgc                                                                                                                                             >  2:698268/1‑150 (MQ=255)
           tGAGGATCGCCGCACTTTGGCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCg                                                                                                                                           <  2:1421677/150‑1 (MQ=255)
                aTCGCCGCACTTTGGCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGGCGACTGGAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGACGGGGcg                                                                                                                                      >  1:1646407/1‑150 (MQ=255)
                   gccgcACTTTGGCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGcgtcg                                                                                                                                   <  2:1180942/150‑1 (MQ=255)
                   gccgcACTTTGGCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGcgtcg                                                                                                                                   <  2:1180369/150‑1 (MQ=255)
                    ccgcACTTTGGCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCTGGCCCGCGcc                                                                                                                                            >  2:1620857/1‑140 (MQ=255)
                    ccgcACTTTGGCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCTGGCCCGCGcc                                                                                                                                            <  1:1620857/140‑1 (MQ=255)
                    ccgcACTTTGGCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGACCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGcgtcgt                                                                                                                                  >  2:1468265/1‑150 (MQ=255)
                     cgcACTTTGGCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGtt                                                                                                                                 <  1:1649834/150‑1 (MQ=255)
                      gcACTTTGGCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTg                                                                                                                                <  1:1179878/150‑1 (MQ=255)
                        tcgtgggCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGAt                                                                                                                              <  2:765922/145‑1 (MQ=255)
                          tttGGCGCGACAGCCTGACGTAGGCCGGGATTTTGCGCAAGTCATCGTGCATCATCGACACATCGGCCGTCTCGATGGCGGTGTCGATGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGc                                                                                                                            >  2:742119/1‑150 (MQ=255)
                              gcgcGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTc                                                                                                                        >  1:489770/1‑150 (MQ=255)
                               cgcgACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCa                                                                                                                       >  2:1139111/1‑150 (MQ=255)
                                        cTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGCGCCAGCGCCGGGGCGTCGTTGCTGCCGTCACCGACCATg                                                                                                              >  2:28937/1‑150 (MQ=255)
                                         tGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGc                                                                                                             <  1:282825/150‑1 (MQ=255)
                                                  ccGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGAt                                                                                                    <  2:1335929/150‑1 (MQ=255)
                                                  ccGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGAt                                                                                                    <  2:489770/150‑1 (MQ=255)
                                                   cGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATg                                                                                                   >  1:131117/1‑150 (MQ=255)
                                                    gggATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATgg                                                                                                  <  2:131117/150‑1 (MQ=255)
                                                      gATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGcc                                                                                                <  2:272200/150‑1 (MQ=255)
                                                        ttttGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGcgt                                                                                                                                     >  1:1855958/1‑111 (MQ=255)
                                                        ttttGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGcgt                                                                                                                                     <  2:1855958/111‑1 (MQ=255)
                                                        ttttGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCtt                                                                                              >  1:793364/1‑150 (MQ=255)
                                                        ttttGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCtt                                                                                              <  1:1139111/150‑1 (MQ=255)
                                                            gcgcAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGc                                                                                          <  2:790853/150‑1 (MQ=255)
                                                               cAAGTCATCGTCCATCAGCGCCACCTCGGCCGTCGCGATGGCGGTGCGGGTTCCGGCAGCAGCCATGGCAAAACCGATTGCGGCGCGGGCCAGCGCCGGGGCGTCGTTGTTGCCGTCACCGACCATGCCCGCCCGATGGCCTTGTGCGTa                                                                                       <  2:816282/150‑1 (MQ=255)
                                                                 aGTCATTGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACa                                                                                     >  2:760519/1‑150 (MQ=255)
                                                                  gTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGAt                                                                                                    >  1:275033/1‑134 (MQ=255)
                                                                  gTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGAt                                                                                                    <  2:275033/134‑1 (MQ=255)
                                                                   tCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGcgcg                                                                                                                                                      >  2:1748603/1‑83 (MQ=255)
                                                                   tCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGcgcg                                                                                                                                                      <  1:1748603/83‑1 (MQ=255)
                                                                     aTCGTCCATCAGCGCCGCATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGc                                                                                 >  1:1315329/1‑150 (MQ=255)
                                                                       cGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCt                                                                               >  1:1160384/1‑150 (MQ=255)
                                                                        gTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTc                                                                              >  2:315328/1‑150 (MQ=255)
                                                                            aTCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGAAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACTATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATg                                                                          <  2:842307/150‑1 (MQ=255)
                                                                               aGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCACTTCCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGtt                                                                       <  1:117188/150‑1 (MQ=255)
                                                                                  gcCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTc                                                                    <  2:1631627/150‑1 (MQ=255)
                                                                                       aTCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGAt                                                                                                    >  1:897539/1‑113 (MQ=255)
                                                                                       aTCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGAt                                                                                                    <  2:897539/113‑1 (MQ=255)
                                                                                        tCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGCGCCAGCGCCGGGGCGTCGTTGATGCCGCCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTg                                                              >  2:1267988/1‑150 (MQ=255)
                                                                                           gCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCTCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTcg                                                           <  2:1211189/150‑1 (MQ=255)
                                                                                           gCCGTCTCGATGGCGGTGACGGTGCTGGCAGCTGCTATGCTAAAACCGAGTTTGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTcg                                                           <  2:1451722/150‑1 (MQ=255)
                                                                                            ccGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGAt                                                                                                    <  1:1848841/108‑1 (MQ=255)
                                                                                            ccGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGAt                                                                                                    >  2:1848841/1‑108 (MQ=255)
                                                                                            ccGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTcgg                                                          >  2:850359/1‑150 (MQ=255)
                                                                                                 tcGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGGTTTCAGCTTGTcggccggc                                                     >  2:61071/1‑150 (MQ=255)
                                                                                                   gATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCCGCTTGTCGGCCGGCac                                                   >  2:478818/1‑149 (MQ=255)
                                                                                                          gtgtCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCtt                                                               <  1:1241460/131‑1 (MQ=255)
                                                                                                          gtgtCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCtt                                                               >  2:1241460/1‑131 (MQ=255)
                                                                                                                     ggCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGcgcg                                 >  1:1437745/1‑150 (MQ=255)
                                                                                                                        agcagcCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCAt                                                                                                               >  2:92647/1‑69 (MQ=255)
                                                                                                                        agcagcCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCAt                                                                                                               <  1:92647/69‑1 (MQ=255)
                                                                                                                             ccATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGccc                                         <  2:1371216/134‑1 (MQ=255)
                                                                                                                             ccATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGccc                                         >  1:1371216/1‑134 (MQ=255)
                                                                                                                                tGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTc                              >  2:1182854/1‑142 (MQ=255)
                                                                                                                                tGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTc                              <  1:1182854/142‑1 (MQ=255)
                                                                                                                                 ggCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTCccac                     <  1:1322076/150‑1 (MQ=255)
                                                                                                                                  gCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTc                         <  2:125392/145‑1 (MQ=255)
                                                                                                                                  gCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTc                         >  1:125392/1‑145 (MQ=255)
                                                                                                                                    aaaaCCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTCCcaccac                  <  2:804416/150‑1 (MQ=255)
                                                                                                                                     aaaCCGATTGCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATCCCCACCACg                 >  2:292205/1‑150 (MQ=255)
                                                                                                                                        ccGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTCCCACCACGgca              <  2:1315329/150‑1 (MQ=255)
                                                                                                                                        ccGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCAACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTCCCACCACGgca              <  2:484656/150‑1 (MQ=255)
                                                                                                                                         cGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGGTTCCCACCACGgccg             >  2:1350273/1‑148 (MQ=255)
                                                                                                                                          gATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTTGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTCCCACCACGgcagc            >  1:1628093/1‑150 (MQ=255)
                                                                                                                                          gATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTCCCACCACGgcagc            >  1:489849/1‑150 (MQ=255)
                                                                                                                                            tttCGGCGCGGGCCGGCGCCGGGGCGTCGTTGAGGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTCCCACCGCGGCAGCaa          <  1:839839/150‑1 (MQ=255)
                                                                                                                                            tttCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCGCCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTCCCACCACGGCAGCaa          <  1:245173/150‑1 (MQ=255)
                                                                                                                                             cccGGCGCTGGCCCGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTCCCACCACGGCAGCAAt         >  1:75558/3‑150 (MQ=255)
                                                                                                                                              ccGGCGCTGGCCCGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGc                                                                                                 <  2:1113488/60‑1 (MQ=38)
                                                                                                                                              ccGGCGCTGGCCCGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGc                                                                                                 >  1:1113488/2‑61 (MQ=38)
                                                                                                                                                ggCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTCCCACCACGGCAGCAATGGc      <  1:1172558/150‑1 (MQ=255)
                                                                                                                                                    cgGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTCCCACCACGGCAGCAATGGCCTgc  <  2:1396733/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GATGTTCTGCATGAGGATCGCCGCACTTTGGCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTCCCACCACGGCAGCAATGGCCTGC  >  NC_002947/5864092‑5864389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑