Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,864,246 A→C 6.5% L654R (CTG→CGG)  cadA‑III ← cadmium translocating P‑type ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,864,2460AC 6.5% 197.1 / 5.8 78L654R (CTG→CGG) cadA‑IIIcadmium translocating P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base A (34/38);  new base C (3/2);  total (37/41)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.21e-01

TCTGCATGAGGATCGCCGCACTTTGGCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTCCCACCACGGCAGCAATGGCCTGCGCA  >  NC_002947/5864097‑5864392
                                                                                                                                                     |                                                                                                                                                  
tCTGCATGAGGATCGCCGCACTTTGGCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCAGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCa                                                                                                                                                    <  1:1678464/150‑1 (MQ=255)
   gCATGAGGATCGCCGCACTTTGGCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCCGCCATGGCAAAACCGATTTCGGCGCGGGCCAgcg                                                                                                                                                 >  1:757579/1‑150 (MQ=255)
    cATGAGGATCGCCGCACTTTGGCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGCGCCAgcgc                                                                                                                                                >  2:698268/1‑150 (MQ=255)
      tGAGGATCGCCGCACTTTGGCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCg                                                                                                                                              <  2:1421677/150‑1 (MQ=255)
           aTCGCCGCACTTTGGCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGGCGACTGGAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGACGGGGcg                                                                                                                                         >  1:1646407/1‑150 (MQ=255)
              gccgcACTTTGGCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGcgtcg                                                                                                                                      <  2:1180942/150‑1 (MQ=255)
              gccgcACTTTGGCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGcgtcg                                                                                                                                      <  2:1180369/150‑1 (MQ=255)
               ccgcACTTTGGCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCTGGCCCGCGcc                                                                                                                                               >  2:1620857/1‑140 (MQ=255)
               ccgcACTTTGGCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCTGGCCCGCGcc                                                                                                                                               <  1:1620857/140‑1 (MQ=255)
               ccgcACTTTGGCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGACCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGcgtcgt                                                                                                                                     >  2:1468265/1‑150 (MQ=255)
                cgcACTTTGGCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGtt                                                                                                                                    <  1:1649834/150‑1 (MQ=255)
                 gcACTTTGGCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTg                                                                                                                                   <  1:1179878/150‑1 (MQ=255)
                   tcgtgggCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGAt                                                                                                                                 <  2:765922/145‑1 (MQ=255)
                     tttGGCGCGACAGCCTGACGTAGGCCGGGATTTTGCGCAAGTCATCGTGCATCATCGACACATCGGCCGTCTCGATGGCGGTGTCGATGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGc                                                                                                                               >  2:742119/1‑150 (MQ=255)
                         gcgcGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTc                                                                                                                           >  1:489770/1‑150 (MQ=255)
                          cgcgACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCa                                                                                                                          >  2:1139111/1‑150 (MQ=255)
                                   cTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGCGCCAGCGCCGGGGCGTCGTTGCTGCCGTCACCGACCATg                                                                                                                 >  2:28937/1‑150 (MQ=255)
                                    tGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGc                                                                                                                <  1:282825/150‑1 (MQ=255)
                                             ccGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGAt                                                                                                       <  2:1335929/150‑1 (MQ=255)
                                             ccGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGAt                                                                                                       <  2:489770/150‑1 (MQ=255)
                                              cGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATg                                                                                                      >  1:131117/1‑150 (MQ=255)
                                               gggATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATgg                                                                                                     <  2:131117/150‑1 (MQ=255)
                                                 gATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGcc                                                                                                   <  2:272200/150‑1 (MQ=255)
                                                   ttttGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGcgt                                                                                                                                        >  1:1855958/1‑111 (MQ=255)
                                                   ttttGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGcgt                                                                                                                                        <  2:1855958/111‑1 (MQ=255)
                                                   ttttGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCtt                                                                                                 <  1:1139111/150‑1 (MQ=255)
                                                   ttttGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCtt                                                                                                 >  1:793364/1‑150 (MQ=255)
                                                       gcgcAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGc                                                                                             <  2:790853/150‑1 (MQ=255)
                                                          cAAGTCATCGTCCATCAGCGCCACCTCGGCCGTCGCGATGGCGGTGCGGGTTCCGGCAGCAGCCATGGCAAAACCGATTGCGGCGCGGGCCAGCGCCGGGGCGTCGTTGTTGCCGTCACCGACCATGCCCGCCCGATGGCCTTGTGCGTa                                                                                          <  2:816282/150‑1 (MQ=255)
                                                            aGTCATTGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACa                                                                                        >  2:760519/1‑150 (MQ=255)
                                                             gTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGAt                                                                                                       >  1:275033/1‑134 (MQ=255)
                                                             gTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGAt                                                                                                       <  2:275033/134‑1 (MQ=255)
                                                                aTCGTCCATCAGCGCCGCATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGc                                                                                    >  1:1315329/1‑150 (MQ=255)
                                                                  cGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCt                                                                                  >  1:1160384/1‑150 (MQ=255)
                                                                   gTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTc                                                                                 >  2:315328/1‑150 (MQ=255)
                                                                       aTCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGAAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACTATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATg                                                                             <  2:842307/150‑1 (MQ=255)
                                                                          aGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCACTTCCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGtt                                                                          <  1:117188/150‑1 (MQ=255)
                                                                             gcCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTc                                                                       <  2:1631627/150‑1 (MQ=255)
                                                                                  aTCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGAt                                                                                                       >  1:897539/1‑113 (MQ=255)
                                                                                  aTCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGAt                                                                                                       <  2:897539/113‑1 (MQ=255)
                                                                                   tCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGCGCCAGCGCCGGGGCGTCGTTGATGCCGCCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTg                                                                 >  2:1267988/1‑150 (MQ=255)
                                                                                      gCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCTCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTcg                                                              <  2:1211189/150‑1 (MQ=255)
                                                                                      gCCGTCTCGATGGCGGTGACGGTGCTGGCAGCTGCTATGCTAAAACCGAGTTTGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTcg                                                              <  2:1451722/150‑1 (MQ=255)
                                                                                       ccGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGAt                                                                                                       >  2:1848841/1‑108 (MQ=255)
                                                                                       ccGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGAt                                                                                                       <  1:1848841/108‑1 (MQ=255)
                                                                                       ccGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTcgg                                                             >  2:850359/1‑150 (MQ=255)
                                                                                            tcGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGGTTTCAGCTTGTcggccggc                                                        >  2:61071/1‑150 (MQ=255)
                                                                                              gATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCCGCTTGTCGGCCGGCac                                                      >  2:478818/1‑149 (MQ=255)
                                                                                                     gtgtCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCtt                                                                  >  2:1241460/1‑131 (MQ=255)
                                                                                                     gtgtCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCtt                                                                  <  1:1241460/131‑1 (MQ=255)
                                                                                                                ggCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGcgcg                                    >  1:1437745/1‑150 (MQ=255)
                                                                                                                   agcagcCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCAt                                                                                                                  >  2:92647/1‑69 (MQ=255)
                                                                                                                   agcagcCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCAt                                                                                                                  <  1:92647/69‑1 (MQ=255)
                                                                                                                        ccATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGccc                                            <  2:1371216/134‑1 (MQ=255)
                                                                                                                        ccATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGccc                                            >  1:1371216/1‑134 (MQ=255)
                                                                                                                           tGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTc                                 >  2:1182854/1‑142 (MQ=255)
                                                                                                                           tGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTc                                 <  1:1182854/142‑1 (MQ=255)
                                                                                                                            ggCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTCccac                        <  1:1322076/150‑1 (MQ=255)
                                                                                                                             gCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTc                            <  2:125392/145‑1 (MQ=255)
                                                                                                                             gCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTc                            >  1:125392/1‑145 (MQ=255)
                                                                                                                               aaaaCCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTCCcaccac                     <  2:804416/150‑1 (MQ=255)
                                                                                                                                aaaCCGATTGCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATCCCCACCACg                    >  2:292205/1‑150 (MQ=255)
                                                                                                                                   ccGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTCCCACCACGgca                 <  2:1315329/150‑1 (MQ=255)
                                                                                                                                   ccGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCAACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTCCCACCACGgca                 <  2:484656/150‑1 (MQ=255)
                                                                                                                                    cGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGGTTCCCACCACGgccg                >  2:1350273/1‑148 (MQ=255)
                                                                                                                                     gATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTTGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTCCCACCACGgcagc               >  1:1628093/1‑150 (MQ=255)
                                                                                                                                     gATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTCCCACCACGgcagc               >  1:489849/1‑150 (MQ=255)
                                                                                                                                       tttCGGCGCGGGCCGGCGCCGGGGCGTCGTTGAGGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTCCCACCGCGGCAGCaa             <  1:839839/150‑1 (MQ=255)
                                                                                                                                       tttCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCGCCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTCCCACCACGGCAGCaa             <  1:245173/150‑1 (MQ=255)
                                                                                                                                        cccGGCGCTGGCCCGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTCCCACCACGGCAGCAAt            >  1:75558/3‑150 (MQ=255)
                                                                                                                                         ccGGCGCTGGCCCGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGc                                                                                                    <  2:1113488/60‑1 (MQ=38)
                                                                                                                                         ccGGCGCTGGCCCGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGc                                                                                                    >  1:1113488/2‑61 (MQ=38)
                                                                                                                                           ggCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTCCCACCACGGCAGCAATGGc         <  1:1172558/150‑1 (MQ=255)
                                                                                                                                               cgGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTCCCACCACGGCAGCAATGGCCTgc     <  2:1396733/150‑1 (MQ=255)
                                                                                                                                                  gCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCAcc                                                                                                           <  2:1554791/45‑1 (MQ=255)
                                                                                                                                                  gCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCAcc                                                                                                           >  1:1554791/1‑45 (MQ=255)
                                                                                                                                                  gCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCACCAGGTTGCCCTCGGCGCGGTCGATTCCCACCACGGCAGCAATGGCCTGCGCa  <  2:1506015/150‑1 (MQ=255)
                                                                                                                                                  gCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTCCCACCACGGCAGCAATGGCCTGCGCa  >  2:1276140/1‑150 (MQ=255)
                                                                                                                                                  gCCAGCGCCGGGGCGTCGTGGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTCCCACCACGGCAGCAATGGCCTGCGCa  <  2:654007/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TCTGCATGAGGATCGCCGCACTTTGGCGCGACAGCCTGACGAAGGCCGGGATTTTGCGCAAGTCATCGTCCATCAGCGCCACATCGGCCGTCTCGATGGCGGTGTCGGTGCCGGCAGCAGCCATGGCAAAACCGATTTCGGCGCGGGCCAGCGCCGGGGCGTCGTTGATGCCGTCACCGACCATGCCCACCCGATGGCCTTGTGCGTACAACGCCTCAATGGTTTTCAGCTTGTCGGCCGGCAACAGGTTGCCCTCGGCGCGGTCGATTCCCACCACGGCAGCAATGGCCTGCGCA  >  NC_002947/5864097‑5864392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑