Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,930,471 G→A 5.1% L153L (CTC→CTT)  PP_5198 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,930,4710GA 5.1% 296.0 / 4.1 99L153L (CTC→CTT) PP_5198hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (47/46);  new base A (2/3);  total (49/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.04e-01

GACCGTCAGCGGGCCACCATCGAGCAACAAAATTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCCAGCAGCGCATCATCGACGTGGAATTCGG  >  NC_002947/5930324‑5930619
                                                                                                                                                   |                                                                                                                                                    
gACCGTCAGCGGGCCACCATCGAGCAACAAAATTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAg                                                                                                                                                    >  1:414601/1‑150 (MQ=255)
 aCCGTCAGCGGGCCACCATCGAGCAACAAAATTTGCAGGCCACGGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGc                                                                                                                                                   <  1:587293/150‑1 (MQ=255)
        tcGGGCAACAATACAGCAACAGAGTTTTCATTCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCCCGCACGGTCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGccagatcg                                                                                                                                            >  2:1366420/2‑144 (MQ=255)
        gCGGGCCACCATCGAGCAACAAAATTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGt                                                                                                                                            >  2:359881/1‑150 (MQ=255)
         cGGGCCACCATCGAGCAACAAAATTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTCAAGCCGCaagcg                                                                                                                                           <  1:369925/150‑5 (MQ=255)
             ccaccaTCGAGCAACAAAATTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAgg                                                                                                                                       <  2:695065/150‑1 (MQ=255)
             ccaccaTCGAGCAACAAAATTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAgg                                                                                                                                       <  1:403194/150‑1 (MQ=255)
              caccaTCGAGCAACAAAATTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAggg                                                                                                                                      >  2:1311188/1‑150 (MQ=255)
              caccaTCGAGCAACAAAATTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAggg                                                                                                                                      >  2:995997/1‑150 (MQ=255)
                ccaTCGAGCAACAAAATTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGtt                                                                                                                                    <  1:1365721/150‑1 (MQ=255)
                 caTCGAGCAACAAAATTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGttt                                                                                                                                   >  1:325356/1‑150 (MQ=255)
                  aTCGAGCAACAAAATTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACAATACCGACACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGtttt                                                                                                                                  >  2:215336/1‑150 (MQ=255)
                    cGAGCAACAAAATTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTAt                                                                                                                                <  2:1732838/150‑1 (MQ=255)
                     gAGCAACAAAATTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATc                                                                                                                               <  2:501061/150‑1 (MQ=255)
                          acaaAATTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGAccc                                                                                                                          <  1:1274819/150‑1 (MQ=255)
                            aaaaTTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCcgc                                                                                                                        >  2:1711854/1‑150 (MQ=255)
                            aaaaTTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCAAGTATAGGGTTTTATCCAccccc                                                                                                                        >  1:1342068/1‑148 (MQ=255)
                             aaaTTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCa                                                                                                                                               <  2:29999/126‑1 (MQ=255)
                             aaaTTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCa                                                                                                                                               >  1:29999/1‑126 (MQ=255)
                                tttGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGcca                                                                                                                    >  1:1648920/1‑150 (MQ=255)
                                     aGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAAc                                                                                                               >  1:1114728/1‑150 (MQ=255)
                                      ggCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACt                                                                                                              >  1:1450018/1‑150 (MQ=255)
                                       gCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGa                                                                                                                             >  2:1168698/1‑134 (MQ=255)
                                       gCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGa                                                                                                                             <  1:1168698/134‑1 (MQ=255)
                                         cACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGGCAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGc                                                                                                           <  2:1407924/150‑1 (MQ=255)
                                              tgGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGgcg                                                                                                      >  1:727860/1‑150 (MQ=255)
                                                gcgcAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCGCCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGcc                                                                                                    >  1:430187/1‑150 (MQ=255)
                                                gcgcAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGtt                                                                                                                                    <  1:157293/118‑1 (MQ=255)
                                                gcgcAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGtt                                                                                                                                    >  2:157293/1‑118 (MQ=255)
                                                  gcAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCAt                                                                                                  >  2:1699134/1‑150 (MQ=255)
                                                     aCGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATcgc                                                                                               <  1:359881/150‑1 (MQ=255)
                                                      cGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATcgcg                                                                                              <  2:60931/150‑1 (MQ=255)
                                                          aaGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGgcg                                                                                                      >  1:459972/1‑138 (MQ=255)
                                                          aaGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGgcg                                                                                                      <  2:459972/138‑1 (MQ=255)
                                                          aaGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGata                                                                                          >  2:1377600/1‑150 (MQ=255)
                                                          aaGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGata                                                                                          >  2:1689255/1‑150 (MQ=255)
                                                          aaGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGata                                                                                          >  2:1693284/1‑150 (MQ=255)
                                                           aGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGatat                                                                                         >  1:1894353/1‑150 (MQ=255)
                                                             gCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGGTATCg                                                                                       >  1:1463231/1‑150 (MQ=255)
                                                               cAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGAc                                                                                     <  1:1699134/150‑1 (MQ=255)
                                                               cAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTAGAATGCGTGTTCAGTTGCGCACCTACTAGTGTGACGACCCCCGCCACCGGCTCGCCGGCGCCATCGCGGATATCGAc                                                                                     <  1:1469952/150‑1 (MQ=255)
                                                                  ggCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCt                                                                                   >  1:1690852/1‑149 (MQ=255)
                                                                  ggCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCt                                                                                   <  2:1690852/149‑1 (MQ=255)
                                                                   gcgcTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGc                                                                                 <  2:727860/150‑1 (MQ=255)
                                                                    cgcTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCCTCATCTCGCCGGCGCCATCGCGGATATCGACCTGCa                                                                                <  1:1808875/150‑1 (MQ=255)
                                                                      cTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCaaa                                                                              <  1:404123/150‑1 (MQ=255)
                                                                       tGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAAc                                                                             >  1:112526/1‑150 (MQ=255)
                                                                         cccACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAAcgc                                                                           <  1:1311188/150‑1 (MQ=255)
                                                                               aTACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCggcagg                                                                     <  1:1689255/150‑1 (MQ=255)
                                                                               aTACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCggcagg                                                                     <  1:1693284/150‑1 (MQ=255)
                                                                                   cGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCAtt                                                                 >  2:670388/1‑150 (MQ=255)
                                                                                     gCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGGGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCGACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTc                                                               >  2:999798/1‑150 (MQ=255)
                                                                                       aCCGACAATCAACAGAGCTGCGCGCAGTGCCATTGCTGACGCCTGCCGCGCTGGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGTCGGCGCCATCGCGGATATCGACCGGCAAACGCGGCTGGTATTTCtg                                                             <  2:1342068/150‑2 (MQ=255)
                                                                                          gACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGcc                                                          >  1:1032213/1‑150 (MQ=255)
                                                                                          gACAATCAACAAGGCTGGGCGCATTTCCATGCCTTACGCCTGCTTCGCTTCCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACTAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGcc                                                          <  2:1000856/150‑1 (MQ=255)
                                                                                           aCAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCg                                                         <  1:215336/150‑1 (MQ=255)
                                                                                                  aCAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATTGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTggcgg                                                  >  2:1586182/1‑150 (MQ=255)
                                                                                                   cAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTggcggc                                                 <  1:822019/150‑1 (MQ=255)
                                                                                                        cTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCctgct                                            >  2:161399/1‑150 (MQ=255)
                                                                                                         tGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCctgctg                                           >  2:1297130/1‑150 (MQ=255)
                                                                                                           cgcgcATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCCGTGGCGGCCTGCTGGc                                         >  2:1435854/1‑150 (MQ=255)
                                                                                                            gcgcATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCg                                        <  1:546504/150‑1 (MQ=255)
                                                                                                               cATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGAt                                     <  2:1450018/150‑1 (MQ=255)
                                                                                                                 tttCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGAttt                                   <  1:1711854/150‑1 (MQ=255)
                                                                                                                     cATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCt                                                                                   <  1:494016/98‑1 (MQ=255)
                                                                                                                     cATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCt                                                                                   >  2:494016/1‑98 (MQ=255)
                                                                                                                     cATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTc                               >  1:302198/1‑150 (MQ=255)
                                                                                                                     cATGCCTTACGCCTGCCTCGCGTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTc                               <  2:675720/150‑1 (MQ=255)
                                                                                                                      aTGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTcc                              >  2:350670/1‑150 (MQ=255)
                                                                                                                      aTGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACGCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTcc                              <  1:389154/150‑1 (MQ=255)
                                                                                                                       tGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCca                             <  2:546085/150‑1 (MQ=255)
                                                                                                                        gCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCcag                            <  1:770213/150‑1 (MQ=255)
                                                                                                                         ccTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCcagc                           >  1:1307687/1‑150 (MQ=255)
                                                                                                                          cTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCg                                        <  2:900007/136‑1 (MQ=255)
                                                                                                                          cTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCg                                        >  1:900007/1‑136 (MQ=255)
                                                                                                                           ttACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCcagcag                         >  2:1900839/1‑150 (MQ=255)
                                                                                                                            tACGCCTGCCTCGCTTGCGGCTGGAGCCGGACGTATAGGGGTGTATCGACTCGCGCCACCAACGCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATATCGTCCagcagc                        >  2:1058007/1‑150 (MQ=255)
                                                                                                                                cctgcctCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCCAGCAGCGcat                    >  1:473408/1‑150 (MQ=255)
                                                                                                                                 ctgcctCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGAc                                                                                     <  1:1428330/84‑1 (MQ=255)
                                                                                                                                 ctgcctCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGAc                                                                                     >  2:1428330/1‑84 (MQ=255)
                                                                                                                                   gcctCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCCagcagc                        <  2:1193456/143‑1 (MQ=255)
                                                                                                                                   gcctCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCCagcagc                        >  1:1193456/1‑143 (MQ=255)
                                                                                                                                   gcctCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCCAGCAGCGcatcat                 <  1:999798/150‑1 (MQ=255)
                                                                                                                                   gcctCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATAGACCTGCAAACGCGGCCGACTTTTCTGGCCGGTTGCGTCCTGCTGGCGGATTTCGTCCAGCAGCGcatcat                 <  1:332023/150‑1 (MQ=255)
                                                                                                                                    cctCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCCAGCAGCGCatcatc                >  2:856419/1‑150 (MQ=255)
                                                                                                                                    cctCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCCAGCAGCGCatcatc                <  2:1648920/150‑1 (MQ=255)
                                                                                                                                    cctCGCTTGCGGCTTGAGCCGCACGTATAGGGCTTTATAGAAAGCAGGCACGTAGGCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATGTCGTCCAGCAGCGCatcatc                >  1:799618/1‑150 (MQ=255)
                                                                                                                                     tatacgtGCGGCTCAAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTc                               >  1:652507/7‑134 (MQ=255)
                                                                                                                                     tatacgtGCGGCTCAAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTc                               <  2:652507/128‑1 (MQ=255)
                                                                                                                                      tCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCCAGCAGCGCATCATCGa              >  1:1322141/1‑150 (MQ=255)
                                                                                                                                       cGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCCAGCAGCGCATCATCGAc             <  2:768687/150‑1 (MQ=255)
                                                                                                                                         cTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCCAGCAGCGCATCATCGACGt           <  2:430187/150‑1 (MQ=255)
                                                                                                                                          ttGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCCAGCAGCGCATCATCGACGTg          <  2:42332/150‑1 (MQ=255)
                                                                                                                                          gtGCGGCTCAAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGAtt                                    >  1:903034/2‑124 (MQ=255)
                                                                                                                                          gtGCGGCTCAAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGAtt                                    <  2:903034/123‑1 (MQ=255)
                                                                                                                                           tGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCCAGCAGCGCATCATCGACGTgg         <  2:1702269/150‑1 (MQ=255)
                                                                                                                                                 ttGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCCAGCAGCGCATCATCGACGTGGAATTCg   >  2:197697/1‑150 (MQ=255)
                                                                                                                                                 ttGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGAACTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCCAGCAGCGCATCATCGACGTGGAATTCg   <  2:1894353/150‑1 (MQ=255)
                                                                                                                                                  tGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCCAGCAGCGCATCATCGACGTGGAATTCgg  <  1:1146329/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GACCGTCAGCGGGCCACCATCGAGCAACAAAATTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCCATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCCAGCAGCGCATCATCGACGTGGAATTCGG  >  NC_002947/5930324‑5930619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑