Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 467,104 C→T 5.3% intergenic (‑34/+19) PP_5445 ← / ← PP_t71 hypothetical protein/tRNA‑Met

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947467,1040CT 5.3% 283.7 / 4.4 94intergenic (‑34/+19)PP_5445/PP_t71hypothetical protein/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base C (42/47);  new base T (2/3);  total (44/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.50e-01

CGCGCTGGGTGACCGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCTGTGCCATCTGAAA  >  NC_002947/466956‑467250
                                                                                                                                                    |                                                                                                                                                  
cgcgcTGGGTGACCGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATcg                                                                                                                                                   <  2:1520046/150‑1 (MQ=255)
     tGGGTGACCGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCgg                                                                                                                                              >  2:237250/1‑150 (MQ=255)
         tGACCGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCGATTGCGCGGCttt                                                                                                                                          <  2:1603629/150‑1 (MQ=255)
           gccGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGt                                                                                                                                        <  1:1459661/149‑1 (MQ=255)
            ccGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTc                                                                                                                                       <  1:1022957/150‑1 (MQ=255)
            ccGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTc                                                                                                                                       <  1:684141/150‑1 (MQ=255)
            ccGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTc                                                                                                                                       <  2:1346204/150‑1 (MQ=255)
                cttcttGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAGAAGCCGCGCAATCGCGCGGCTTTGTCGTAt                                                                                                                                   >  1:1323231/1‑150 (MQ=255)
                 ttcttGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATc                                                                                                                                  <  2:532581/150‑1 (MQ=255)
                  tcttGGAGGGGCAGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGAAAATTTATTTCTTGCGTGCATGCAAAAGCCGCGCAACCGCGCGCCTCTGTCGTacag                                                                                                                                 >  2:227461/1‑147 (MQ=255)
                      ggtggGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCtggtc                                                                                                                             <  1:1007117/150‑2 (MQ=255)
                       gtggGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCtggtgg                                                                                                                            <  1:695904/150‑1 (MQ=255)
                          gggCTGATTTCGCTGAGATACCTTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGcc                                                                                                                         <  2:148292/150‑1 (MQ=255)
                                aTTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCtg                                                                                                                                >  2:1901400/1‑137 (MQ=255)
                                aTTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCtg                                                                                                                                <  1:1901400/137‑1 (MQ=255)
                                   tCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGAc                                                                                                                <  1:240634/150‑1 (MQ=255)
                                     gcggagaTACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGAAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTc                                                                                                              <  2:853228/147‑1 (MQ=255)
                                     gCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCtgg                                                                                                                               >  2:1768655/1‑133 (MQ=255)
                                     gCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCtgg                                                                                                                               <  1:1768655/133‑1 (MQ=255)
                                     gCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTc                                                                                                              >  1:186499/1‑150 (MQ=255)
                                      cTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCg                                                                                                             >  2:823742/1‑150 (MQ=255)
                                       tGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGcc                                                                                                                         <  2:842772/137‑1 (MQ=255)
                                       tGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGcc                                                                                                                         >  1:842772/1‑137 (MQ=255)
                                         agaTACCCTTTTAGCGGTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAAc                                                                                                          <  2:974932/150‑1 (MQ=255)
                                             aCCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTg                                                                                                      <  2:1665018/150‑1 (MQ=255)
                                               cGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGa                                                                                                    <  1:823742/150‑1 (MQ=255)
                                               cGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGa                                                                                                    >  2:279835/1‑150 (MQ=255)
                                               cGTTTTAGCGCTACTTCCAAGGTCAGGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGa                                                                                                    <  2:855656/150‑1 (MQ=255)
                                                gTTTTAGCGCTACTGCCAAGGTAATGCGCGCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCCTTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGAc                                                                                                   <  1:227461/150‑1 (MQ=255)
                                                      gcgcTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCca                                                                                                                       <  2:41722/124‑1 (MQ=255)
                                                      gcgcTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCca                                                                                                                       >  1:41722/1‑124 (MQ=255)
                                                        gcTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGAt                                                                                           >  1:1576268/1‑150 (MQ=255)
                                                          tACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCGATTGCGCGGc                                                                                                                                             <  2:576859/98‑1 (MQ=255)
                                                          tACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCGATTGCGCGGc                                                                                                                                             >  1:576859/1‑98 (MQ=255)
                                                          tACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCaaa                                                                                               >  2:829675/1‑144 (MQ=255)
                                                          tACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCaaa                                                                                               <  1:829675/144‑1 (MQ=255)
                                                            cTNCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTANGCGCCCNTGACCATTTATTTCTTGCGGGCATTAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATg                                                                                       >  1:1195221/1‑150 (MQ=255)
                                                                 aaGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCTCCCCTGACCATTTAGGTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTcc                                                                                  >  2:1551062/1‑150 (MQ=255)
                                                                 aaGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCGATTGCGCGGc                                                                                                                                             >  1:1781949/1‑91 (MQ=255)
                                                                 aaGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCGATTGCGCGGc                                                                                                                                             <  2:1781949/91‑1 (MQ=255)
                                                                 aaGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTc                                                                                                              >  2:1133336/1‑122 (MQ=255)
                                                                 aaGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTc                                                                                                              <  1:1133336/122‑1 (MQ=255)
                                                                 aaGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAg                                                                                     >  2:1419272/1‑147 (MQ=255)
                                                                 aaGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAg                                                                                     <  1:1419272/147‑1 (MQ=255)
                                                                   ggTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCctc                                                                                <  2:1099272/150‑1 (MQ=255)
                                                                     tCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGa                                                                                                    <  2:454010/128‑1 (MQ=255)
                                                                     tCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGa                                                                                                    >  1:454010/1‑128 (MQ=255)
                                                                          cgcTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTc                                                                                                                                       >  1:1029931/1‑88 (MQ=255)
                                                                          cgcTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTc                                                                                                                                       <  2:1029931/88‑1 (MQ=255)
                                                                             tCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAAcc                                                                      >  2:295332/1‑150 (MQ=255)
                                                                                         cgctgGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGa                                                                                                                 >  2:15878/1‑95 (MQ=255)
                                                                                         cgctgGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGa                                                                                                                 <  1:15878/95‑1 (MQ=255)
                                                                                         cgctgGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCtata                                                          <  2:1576268/150‑1 (MQ=255)
                                                                                          ggtgGTTGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACAAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAg                                                         <  2:9210/148‑1 (MQ=255)
                                                                                             ggATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGcc                                                      >  1:1638067/1‑150 (MQ=255)
                                                                                                  tACGCGCCCCTGACCATTTGTTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAg                                                 >  2:243739/1‑150 (MQ=255)
                                                                                                  tACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAg                                                 <  1:772374/150‑1 (MQ=255)
                                                                                                   aCGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCGATAGGCCCTCAGt                                                >  2:1786891/1‑150 (MQ=255)
                                                                                                    cgcgCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTa                                               >  2:1063082/1‑150 (MQ=255)
                                                                                                       gcCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGt                                            <  1:1063082/150‑1 (MQ=255)
                                                                                                       gcCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGt                                            >  1:1702459/1‑150 (MQ=255)
                                                                                                         cccTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCtggt                                                                                                                              <  2:1147901/66‑1 (MQ=255)
                                                                                                         cccTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCtggt                                                                                                                              >  1:1147901/1‑66 (MQ=255)
                                                                                                         cccTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTgg                                          <  2:1015132/150‑1 (MQ=255)
                                                                                                         cccTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTgg                                          <  2:308165/150‑1 (MQ=255)
                                                                                                          ccTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTggg                                         >  2:425234/1‑150 (MQ=255)
                                                                                                           cTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTgggg                                        >  1:924217/1‑149 (MQ=255)
                                                                                                           cTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGc                                        >  1:924224/1‑150 (MQ=255)
                                                                                                           cTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGc                                        <  1:237250/150‑1 (MQ=255)
                                                                                                            tGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAgg                                                        <  1:903635/133‑1 (MQ=255)
                                                                                                            tGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAgg                                                        >  2:903635/1‑133 (MQ=255)
                                                                                                              aCCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGa                                     <  2:1474205/150‑1 (MQ=255)
                                                                                                                 atttatttCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGTCGGATta                                  >  1:186669/1‑150 (MQ=255)
                                                                                                                  tttacttCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGTTATAGGCCCTCAGTAAGTGGGCGGATtat                                 <  1:279835/150‑1 (MQ=255)
                                                                                                                    tatttCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCa                                                  <  1:1610476/131‑1 (MQ=255)
                                                                                                                    tatttCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCa                                                  >  2:1610476/1‑131 (MQ=255)
                                                                                                                       ttCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTGTGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGa                            >  2:1598979/1‑150 (MQ=255)
                                                                                                                        tCTTGCGGGCATGAAGAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAg                           <  2:1323231/150‑1 (MQ=255)
                                                                                                                        tCTTGCGGGCATGAACAAGCCGCGCAATCGCGCGGCTTTGTCATATCTGGTTGCCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAg                           <  2:1534460/150‑1 (MQ=255)
                                                                                                                          ttGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTGAGTGGGCGGATTATAACGAggg                         >  2:951372/1‑150 (MQ=255)
                                                                                                                          ttGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAggg                         >  1:531301/1‑150 (MQ=255)
                                                                                                                            gCGGGCATTAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGNGtt                       <  2:1195221/150‑1 (MQ=255)
                                                                                                                               ggCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTTAGCGATAGTCCCTCAGCAAGTGGGCGGATCATAACGAGGGTTTCg                    >  2:252509/1‑150 (MQ=255)
                                                                                                                                 cATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAAc                              <  2:1369675/138‑1 (MQ=255)
                                                                                                                                 cATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAAc                              >  1:1369675/1‑138 (MQ=255)
                                                                                                                                   tGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAAc                                                                       >  1:759969/1‑95 (MQ=255)
                                                                                                                                   tGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAAc                                                                       <  2:759969/95‑1 (MQ=255)
                                                                                                                                      aaaaGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCtgt             <  2:1702459/150‑1 (MQ=255)
                                                                                                                                      aaaaGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCtgt             >  1:1904099/1‑150 (MQ=255)
                                                                                                                                           ccGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCTGTGCCAt        <  1:1346753/150‑1 (MQ=255)
                                                                                                                                            cgcgCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCTGTGCCATc       >  1:1242203/1‑150 (MQ=255)
                                                                                                                                             gcgcAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACCCGAAACGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCTGTGCCATCt      <  1:1551656/150‑1 (MQ=255)
                                                                                                                                                 aaTCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATAAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAAGGTTTCGCACCTGTGCCATCTGaaa  >  2:363711/1‑150 (MQ=255)
                                                                                                                                                   tCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCtgt             >  1:1756811/1‑137 (MQ=255)
                                                                                                                                                   tCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCtgt             <  2:1756811/137‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGCGCTGGGTGACCGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCTGTGCCATCTGAAA  >  NC_002947/466956‑467250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑