Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 492,311 T→C 7.5% D38G (GAC→GGC)  lptD ← LPS‑assembly protein LptD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947492,3110TC 7.5% 226.6 / 5.9 80D38G (GAC→GGC) lptDLPS‑assembly protein LptD
Reads supporting (aligned to +/- strand):  ref base T (35/39);  new base C (3/3);  total (38/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

GCACGGGCCGGTCCGCAGTTTCGGCTTCGCCCGCCGCTTCGGTGCCGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTCTACGAAACGCGGGGGATTTCAATGCCATCTTATTAGTCCGGGCTTCCTGCGTGCCATCTGCCCG  >  NC_002947/492165‑492459
                                                                                                                                                  |                                                                                                                                                    
gCACGGGCCGGTCCGCAGTTTCGGCTTCGCCCGCCGCTTCGGTGCCGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGa                                                                                                                                                   >  1:1087525/1‑150 (MQ=255)
 cACGGGCCGGTCCGCAGTTTCGGCTTCGCCCGCCGCTTCGGTGCCGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGaa                                                                                                                                                  <  2:1475479/150‑1 (MQ=255)
 cACGGGCCGGTCCGCAGTTTCGGCTTCGCCCGCCGCTTCGGTGCCGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGaa                                                                                                                                                  <  1:1442409/150‑1 (MQ=255)
       ccGGTCCGCAGTTTCGGCTTCGCCCGCCGCTTCGGTGCCGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTc                                                                                                                                            >  1:1352523/1‑150 (MQ=255)
        cGGTCCGCAGTTTCGGCTTCGCCCGCCGCTTCGGTGCCGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCg                                                                                                                                           <  1:1750607/150‑1 (MQ=255)
           tCCGCAGTTTCGGCTTCGCCCGCCGCTTCGGTGCCGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCt                                                                                                                                        >  2:1601381/1‑150 (MQ=255)
           tCCGCAGTTTCGGCTTCGCCCGCCGCTTCGGTGCCGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGCCGAACAGTTCGACt                                                                                                                                        >  2:1613717/1‑150 (MQ=255)
             cGCAGTTTCGGCTTCGCCCGCCGCTTCGGTGCCGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGc                                                                                                                                      >  1:1904289/1‑150 (MQ=255)
             cGCAGTTTCGGCTTCGCCCGCCGCTTCGGTGCCGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGc                                                                                                                                      <  1:629274/150‑1 (MQ=255)
             cGCAGTTTCGGCTTCGCCCGCCGCTTCGGTGCCGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGc                                                                                                                                      <  2:1087525/150‑1 (MQ=255)
             cGCAGTTTCGGCTTCGCCCGCCGCTTCGGTGCCGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGCTGTTGACCGGGGTTTTGGGCTCGCAGCCCCAACCACCGCCGTCGCCCACTTGGCAGTCGAACTGTTCGGCTGc                                                                                                                                      <  2:819616/150‑1 (MQ=255)
              gCAGTTTCGGCTTCGCCCGCCGCTTCGGTGCCGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGCCGAACAGTTCGACTGcc                                                                                                                                     <  1:1613717/150‑1 (MQ=255)
              gCAGTTTCGGCTTCGCCCGCCGCTTCGGTGCCGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTCACCGGTGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGcc                                                                                                                                     >  1:1555094/1‑150 (MQ=255)
                tggttCGGGTTCGGCCGGCGGTTCGGTGGCGGGGCCGGCGGCTGGCCCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGCCGAACAGTTCGACTGCCaa                                                                                                                                   <  2:1327269/147‑2 (MQ=255)
                  tttCGGCTTCGCCCGCCGCTTCGGTGCCGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCt                                                                                                                                        <  1:418788/143‑1 (MQ=255)
                  tttCGGCTTCGCCCGCCGCTTCGGTGCCGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCt                                                                                                                                        >  2:418788/1‑143 (MQ=255)
                  tttCGGCTTCGCCCGCCGCTTCGGTGCCGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGCCGGACACTTGGCAGCCGACCAGTTCGACTGCCaagg                                                                                                                                 >  1:1327269/1‑147 (MQ=255)
                       gCTTCGCCCGCCGCTTCGGTGCCGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTAt                                                                                                                            <  1:983879/150‑1 (MQ=255)
                       gCTTCGCCCGCCGCTTCGGTGCCGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGCCGAACAGTTCGACTGCCaa                                                                                                                                   <  2:10350/143‑2 (MQ=255)
                       gCTTCGCCCGCCGCTTCGGTGCCGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGCCGAACAGTTCGACTGCCaa                                                                                                                                   >  1:10350/1‑142 (MQ=255)
                        cTTCGCCCGCCGCTTCGGTGCCGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATg                                                                                                                           >  2:484299/1‑150 (MQ=255)
                         ttCGCCCGCCGCTTCGGTGCCGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCg                                                                                                                                           >  1:1460208/1‑133 (MQ=255)
                         ttCGCCCGCCGCTTCGGTGCCGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCg                                                                                                                                           <  2:1460208/133‑1 (MQ=255)
                         ttCGCCCGCCGCTGCGGTGGCGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGa                                                                                                                          >  2:787990/1‑150 (MQ=255)
                              ccgccgCTTCGGTGCCGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGgggg                                                                                                                     >  1:754178/1‑150 (MQ=255)
                                 ccgcTTCGGTGCCGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCa                                                                                                                  <  2:1897778/150‑1 (MQ=255)
                                   gcTTCGGTGCCGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCaga                                                                                                                <  2:1280025/150‑1 (MQ=255)
                                    cTTCGGTGCCGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCagag                                                                                                               <  1:526514/150‑1 (MQ=255)
                                      gcGGTGCCGGCGCTGACGGGTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAGCCACCGGCGGCGGACACTTGGCAGTCGACCTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGt                                                                                                             <  2:704317/149‑1 (MQ=255)
                                            ccGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAgg                                                                                                       <  2:1352523/150‑1 (MQ=255)
                                               gAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGcc                                                                                                    <  2:164467/150‑1 (MQ=255)
                                                aGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCca                                                                                                   <  2:1904289/150‑1 (MQ=255)
                                                 gCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCcag                                                                                                  <  1:364424/150‑1 (MQ=255)
                                                  cTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCcagc                                                                                                 <  1:484299/150‑1 (MQ=255)
                                                          tGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGAccgcc                                                                                         >  1:597325/1‑150 (MQ=255)
                                                          tGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGAccgcc                                                                                         >  2:1156026/1‑150 (MQ=255)
                                                           gCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGAccgccg                                                                                        <  1:1414879/150‑1 (MQ=255)
                                                               cTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCg                                                                                                                                           <  1:1126777/95‑1 (MQ=255)
                                                               cTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCg                                                                                                                                           >  2:1126777/1‑95 (MQ=255)
                                                               cTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCagag                                                                                                               >  1:1460340/1‑123 (MQ=255)
                                                               cTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCagag                                                                                                               <  2:1460340/123‑1 (MQ=255)
                                                                ttCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTAc                                                                                   <  1:271923/150‑1 (MQ=255)
                                                                       aCCGGCCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAAc                                                                            <  2:243268/150‑1 (MQ=255)
                                                                           ggCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGaa                                                                        >  1:733831/1‑150 (MQ=255)
                                                                                ggTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACttt                                                                   >  1:1464313/1‑150 (MQ=255)
                                                                                  tGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTCt                                                                 >  1:1460131/1‑150 (MQ=255)
                                                                                    gCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTc                                                                  <  1:869751/147‑1 (MQ=255)
                                                                                    gCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTc                                                                  >  2:869751/1‑147 (MQ=255)
                                                                                    gCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTCTAc                                                               >  2:1699831/1‑150 (MQ=255)
                                                                                        gttgttGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTCTACGaaa                                                           >  1:1425094/1‑150 (MQ=255)
                                                                                            ttgACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTCTACGAAAcgcg                                                       >  1:282623/1‑150 (MQ=255)
                                                                                              gACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAgg                                                                                                       >  2:629904/1‑100 (MQ=255)
                                                                                              gACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAgg                                                                                                       <  1:629904/100‑1 (MQ=255)
                                                                                              gACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTCTACGAAACGCggg                                                     <  2:1137394/150‑1 (MQ=255)
                                                                                              gACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTCTACGAAACGCggg                                                     <  1:245916/150‑1 (MQ=255)
                                                                                               aCTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTCTACGAAACGCgggg                                                    >  2:622133/1‑150 (MQ=255)
                                                                                                   gggTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTCTACGAAACGCGGGGGAtt                                                >  1:377596/1‑150 (MQ=255)
                                                                                                      ttttGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGc                                                                                                                    >  2:779689/1‑79 (MQ=255)
                                                                                                      ttttGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGc                                                                                                                    <  1:779689/79‑1 (MQ=255)
                                                                                                      ttttAGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTCTACGAAACGCGGGGGATTTCa                                             >  2:1817560/1‑150 (MQ=255)
                                                                                                       tttGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTCTACGAAACGCGGGGGATTTCaa                                            <  2:597325/150‑1 (MQ=255)
                                                                                                         tGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTCTACGAAACGCGGGGGATTTCAATg                                          <  1:1156026/150‑1 (MQ=255)
                                                                                                            gCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTCTACGAAACGCGGGGGATTTCAATGCCa                                       >  1:150042/1‑150 (MQ=255)
                                                                                                             cTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTCTACGAAACGCGGGGGATTTCAATGCCAt                                      <  2:150042/150‑1 (MQ=255)
                                                                                                             cTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTCTACGAAACGCGGGGGATTTCAATGCCAt                                      <  1:787990/150‑1 (MQ=255)
                                                                                                              ttGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTCTACGAAACGCGGGGGATTTCAATGCCATc                                     <  2:1464313/150‑1 (MQ=255)
                                                                                                                  aGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTCTACGAAACGCGGGGGATTTCAATGCCATCttat                                 <  2:89302/150‑1 (MQ=255)
                                                                                                                   gTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTCTACGAAACGCGGGGGATTTCAATGCCATCttatt                                >  1:1016329/1‑150 (MQ=255)
                                                                                                                    tCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTCTACGAAACGCGGGGGATTTCAATGCCATCttatta                               <  2:1721218/150‑1 (MQ=255)
                                                                                                                      ccAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTCTACGAAACGCGGGGGATTTCAATGCCATCTTATTAGt                             >  2:1805036/1‑150 (MQ=255)
                                                                                                                       cAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCaga                                                                                                                <  2:389670/66‑1 (MQ=255)
                                                                                                                       cAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCaga                                                                                                                >  1:389670/1‑66 (MQ=255)
                                                                                                                       cAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTCTACGAAACGCGGGGGATTTCAATGCCATCTTATTAGTc                            >  2:851175/1‑150 (MQ=255)
                                                                                                                              cggcggcggACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTCTACGAAACGCGGGGGATTTCAATGc                                         >  2:1661711/1‑130 (MQ=255)
                                                                                                                              cggcggcggACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTCTACGAAACGCGGGGGATTTCAATGc                                         <  1:1661711/130‑1 (MQ=255)
                                                                                                                                 cggcggACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTCTACGAAACGCGGGGGATTTCAATGCCATCTTATTAGTCCGGGCTTCCt                  <  1:1621212/150‑1 (MQ=255)
                                                                                                                                     ggACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGACAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTCTACGAAACGCGGGGGATTTCAATGCCATCTTATTAGTCCGGGCTTCCTGCGt              >  2:456873/1‑150 (MQ=255)
                                                                                                                                         acTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTCTACGAAACGCGGGGGATTTCAATGCCATCTTATTAGTCCGGGCTTCCTGCGTGCCa          <  1:851175/150‑1 (MQ=255)
                                                                                                                                           ttGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTCTACGAAACGCGGGGGATTTCAATGCCATCTTATTAGTCCGGGCTTCCTGCGTGCCATc        >  2:786016/1‑150 (MQ=255)
                                                                                                                                                 gTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTCTACGAAACGCGGGGGATTTCAATGCCATCTTATTAGTCCGGGCTTCCTGCGTGCCATCTGCCcg  >  2:457368/1‑150 (MQ=255)
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GCACGGGCCGGTCCGCAGTTTCGGCTTCGCCCGCCGCTTCGGTGCCGGAGCTGACGGCTGCACCTTCGTGCACCGGGCGCGGTGGCAAGTTGTTGACTGGGGTTTTGGGCTTGCAGTCCCAACCACCGGCGGCGGACACTTGGCAGTCGAACTGTTCGGCTGCCACTACGTATGAGGTGGCCAGAGGTTGCAGGGCCAGCAGACCGCCGGTTACCAGCAACGGAAACTTTCTACGAAACGCGGGGGATTTCAATGCCATCTTATTAGTCCGGGCTTCCTGCGTGCCATCTGCCCG  >  NC_002947/492165‑492459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑