BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	593,437	A→C	5.0%	E391A (GAG→GCG)	PP_0503 →	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	593,437	0	A	C	5.0%	295.7 / 3.5	107	E391A (GAG→GCG)	PP_0503	MFS transporter
Reads supporting (aligned to +/- strand): ref base A (42/59); new base C (3/3); total (45/62)
Fisher's exact test for biased strand distribution p-value = 6.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

TTGCTATTCGCTGGGCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCG  >  NC_002947/593288‑593576

ttGCTATTCGCTGGGCAGCGGTTTGGGGGCGGTGTTGGCGTGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCAGCAGTGCTGCCGCACTGTGCTACTGGCTATGGCCGCAACCCCCGGGGGCGGCCGa                                                                                                                                             >  2:656192/1‑150 (MQ=255)

    tATTCGCTGGGCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGgcc                                                                                                                                         >  2:1899872/1‑150 (MQ=255)

     aTTCGCTGGGCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAGCGCGCGAGGGCGGCCGAGgccg                                                                                                                                        >  1:696078/1‑150 (MQ=255)

     aTTCGCTGGGCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGgccg                                                                                                                                        >  1:183574/1‑150 (MQ=255)

       tCGCTGGGCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGgccgcc                                                                                                                                      <  2:767323/150‑1 (MQ=255)

       tCGCTGGGCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCCGCGCTACTGGTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCTCGgccgcc                                                                                                                                      <  1:1278293/150‑1 (MQ=255)

        cGCTGGGCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGTCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGccc                                                                                                                                     >  1:596090/1‑150 (MQ=255)

         gCTGGGCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGGGTTTGCACACTGGGGCTGGGTTGGGGTGTTTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGCGGGCGGCCGAGGCCGCCCt                                                                                                                                    >  2:852851/1‑150 (MQ=255)

           tGGGTAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGc                                                                                                                                  <  2:173205/150‑1 (MQ=255)

           tGGGCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGc                                                                                                                                  >  2:62745/1‑150 (MQ=255)

            gggCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCa                                                                                                                                 <  2:16545/150‑1 (MQ=255)

            gggCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTCTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCTCGGCCGCCCtcgc                                                                                                                                 <  1:1904925/150‑4 (MQ=255)

             ggCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCaa                                                                                                                                >  1:1032288/1‑150 (MQ=255)

                aGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGt                                                                                                                             >  1:1164306/1‑150 (MQ=255)

                aGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGt                                                                                                                             >  1:1071045/1‑150 (MQ=255)

                   ggTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCa                                                                                                                          >  1:724761/1‑150 (MQ=255)

                    gTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAg                                                                                                                         <  2:1164306/150‑1 (MQ=255)

                    gTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAg                                                                                                                         <  1:200808/150‑1 (MQ=255)

                    gTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAg                                                                                                                         <  1:1620406/150‑1 (MQ=255)

                      ttGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGt                                                                                                                       <  2:1815742/150‑1 (MQ=255)

                         ggggCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAg                                                                                                                    <  2:753111/150‑1 (MQ=255)

                           ggCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGaa                                                                                                                  >  2:163988/1‑150 (MQ=255)

                           ggCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGaa                                                                                                                  <  2:1711670/150‑1 (MQ=255)

                              ggTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACtt                                                                                                               <  1:163988/150‑1 (MQ=255)

                              ggTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACtt                                                                                                               <  2:6844/150‑1 (MQ=255)

                              ggTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACtt                                                                                                               <  1:1311389/150‑1 (MQ=255)

                                 gtTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTa                                                                                                            <  2:1868499/150‑1 (MQ=255)

                                  ttGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGGGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAg                                                                                                           >  2:1398019/1‑150 (MQ=255)

                                      cGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCaa                                                                                                       <  1:1315314/150‑1 (MQ=255)

                                         gCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAAATTGTAGCCACgac                                                                                                    <  2:1324614/150‑1 (MQ=255)

                                           tGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAgaccg                                                                                                  >  1:740980/1‑150 (MQ=255)

                                            gggTGTGTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCCGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAgaccga                                                                                                 <  2:574233/150‑1 (MQ=255)

                                             ggTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAgaccgac                                                                                                >  1:1771967/1‑150 (MQ=255)

                                             ggTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAATGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAgaccgac                                                                                                <  2:1032288/150‑1 (MQ=255)

                                               tgtTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCa                                                                                              >  1:1500719/1‑150 (MQ=255)

                                               tgtTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGAACGAACa                                                                                              <  2:473947/150‑1 (MQ=255)

                                                 tttNCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATg                                                                                            >  2:1202972/1‑150 (MQ=255)

                                                 tttGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATg                                                                                            <  2:1871300/150‑1 (MQ=255)

                                                    gCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGtat                                                                                         >  1:508126/1‑150 (MQ=255)

                                                      acacTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATAc                                                                                       <  2:838848/150‑1 (MQ=255)

                                                      acacTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATAc                                                                                       >  1:1384667/1‑150 (MQ=255)

                                                      acacTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATAc                                                                                       <  1:1346461/150‑1 (MQ=255)

                                                        acTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCAGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATAccc                                                                                     >  1:20124/1‑150 (MQ=255)

                                                          tGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCAc                                                                                   <  1:1202972/150‑1 (MQ=255)

                                                            gggCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACgg                                                                                 <  2:1771967/150‑1 (MQ=255)

                                                            gggCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACgg                                                                                 <  1:113599/150‑1 (MQ=255)

                                                             ggCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACggg                                                                                >  1:1137227/1‑150 (MQ=255)

                                                             ggCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACggg                                                                                <  1:1027046/150‑1 (MQ=255)

                                                               cTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtc                                                                              <  2:1210958/150‑1 (MQ=255)

                                                                tGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAg                                                                                                                         <  2:1351545/106‑1 (MQ=255)

                                                                tGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAg                                                                                                                         >  1:1351545/1‑106 (MQ=255)

                                                                  ggTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgac                                                                           <  2:1873143/150‑1 (MQ=255)

                                                                   gTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACtt                                                                                                               <  2:1701304/113‑1 (MQ=255)

                                                                         gtgtgtGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgacgtcgacg                                                                    >  1:597213/1‑150 (MQ=255)

                                                                          tgtgtgGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgacgtcgacgt                                                                   >  1:1703558/1‑150 (MQ=255)

                                                                            tgtgGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGCGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgacgtcgacgtcg                                                                 >  2:878176/1‑150 (MQ=255)

                                                                              tgGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgacgtcgacgtcgac                                                               <  2:490362/150‑1 (MQ=255)

                                                                                ggCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCt                                                             <  1:7455/150‑1 (MQ=255)

                                                                                 gCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCtt                                                            <  2:1500719/150‑1 (MQ=255)

                                                                                  tgtGGCAGGGGGATCAGTGCCGCCGCACTGTGCCACTGGGTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCGTTGTCCAGGTCAGAGGTGGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGGCGACGTCGACCTTg                                                           <  2:623429/148‑1 (MQ=255)

                                                                                   ttGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGAcccgtg                                                          <  1:641495/150‑5 (MQ=255)

                                                                                   ttGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACAGGTCGACGTCGACGTCGAcccgtg                                                          <  2:1468889/150‑5 (MQ=255)

                                                                                   ttGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACCTGTAGCCAAGTCCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTgg                                                          <  1:1181424/150‑1 (MQ=255)

                                                                                   ttGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCAAGGGGGGCCGAAGCCGGCCGGCAGGGTGCGGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGCGTGCGTCGACGTCGACCTTgg                                                          >  1:1039049/1‑150 (MQ=255)

                                                                                      gCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCt                                                       >  2:1184119/1‑150 (MQ=255)

                                                                                        aTGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATATGTAGGCACGGGTCGACGTCGACGTCGACCTTGGTCTTg                                                     >  1:1534360/1‑150 (MQ=255)

                                                                                        aTGGGCATCAGTGCCGCCGCACTGTGCTAATGGCTATGGCTGCAGCTCGCAATCGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTg                                                     <  2:50545/150‑1 (MQ=255)

                                                                                          gggCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCATAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCt                                                   <  2:1902896/150‑1 (MQ=255)

                                                                                           ggCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTg                                                  >  2:1556021/1‑150 (MQ=255)

                                                                                            gCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGt                                                 >  2:1364371/1‑150 (MQ=255)

                                                                                             cATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTa                                                >  1:476465/1‑150 (MQ=255)

                                                                                             cATCAGGGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTa                                                <  1:266265/150‑1 (MQ=255)

                                                                                               acAGTGCGGCGGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGc                                              >  1:4728/2‑150 (MQ=255)

                                                                                                    gccgccGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAgg                                         <  1:1207418/150‑1 (MQ=255)

                                                                                                       gccgcACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCg                                      >  1:914381/1‑150 (MQ=255)

                                                                                                       gccgcACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCg                                      <  2:476465/150‑1 (MQ=255)

                                                                                                                 gCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGt                            <  1:1899872/150‑1 (MQ=255)

                                                                                                                    aCTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCCCGGGTCGACGTCGACGTCGACCTTGCTCTTGCTGGAGCCAAGGTCGGTCTGGCCGTTGa                         <  2:20124/150‑1 (MQ=255)

                                                                                                                     cTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAGCTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGAc                        <  1:313098/150‑1 (MQ=255)

                                                                                                                     cTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcga                                                                            >  2:871799/1‑98 (MQ=255)

                                                                                                                     cTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCaa                                           <  1:789892/131‑1 (MQ=255)

                                                                                                                     cTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCaa                                           >  2:789892/1‑131 (MQ=255)

                                                                                                                      tGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACg                       >  1:383894/1‑150 (MQ=255)

                                                                                                                        gCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtc                                                                              >  1:160677/1‑93 (MQ=255)

                                                                                                                        gCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtc                                                                              <  2:160677/93‑1 (MQ=255)

                                                                                                                             ggCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCt                >  1:1617909/1‑150 (MQ=255)

                                                                                                                              gCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCtt               <  1:477333/150‑1 (MQ=255)

                                                                                                                               cTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgacgtcgacgtc                                                                  >  2:124084/1‑98 (MQ=255)

                                                                                                                                tGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTgg             >  2:184638/1‑150 (MQ=255)

                                                                                                                                  cAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGATGTCGACCTTGGTCTTGc                                                    <  2:1148637/109‑1 (MQ=255)

                                                                                                                                  cAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGATGTCGACCTTGGTCTTGc                                                    >  1:1148637/1‑109 (MQ=255)

                                                                                                                                    aCGCGCGAGGGCGGGCGAGGACGACCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtc         <  2:779336/150‑1 (MQ=255)

                                                                                                                                      gcgcgAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcga       <  1:852851/150‑1 (MQ=255)

                                                                                                                                       ggcgAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcgat      <  1:656192/149‑1 (MQ=255)

                                                                                                                                       cgcgAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcgat      <  2:1617909/150‑1 (MQ=255)

                                                                                                                                          gAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcgatgtc   >  1:1165727/1‑150 (MQ=255)

                                                                                                                                          gAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcgatgtc   <  2:1137227/150‑1 (MQ=255)

                                                                                                                                           aGGGCGGCCTCGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCACGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcgatgtcg  >  1:646790/1‑150 (MQ=255)

                                                                                                                                           aGGGCGGCCTCGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCgg                                 >  2:107380/1‑119 (MQ=255)

                                                                                                                                           aGGGCGGCCTCGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCgg                                 <  1:107380/119‑1 (MQ=255)

TTGCTATTCGCTGGGCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCG  >  NC_002947/593288‑593576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`