Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 944,829 A→T 6.2% S266T (TCG→ACG)  PP_0806 ← surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947944,8290AT 6.2% 227.8 / 2.6 74S266T (TCG→ACG) PP_0806surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base A (29/40);  new base T (2/3);  total (31/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGATATTGCCAGCCGCGTCTGCGGCGCTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGACATCAAGGTTTT  >  NC_002947/944681‑944978
                                                                                                                                                    |                                                                                                                                                     
gagaTATTGCCAGCCGCGTCTGCGGCGCTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAg                                                                                                                                                      >  1:726443/1‑150 (MQ=255)
 agaTATTGCCAGCCGCGTCTGCGGCGCTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCCCCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGc                                                                                                                                                     <  2:53756/150‑1 (MQ=255)
       tGCCAGCCGCGTCTGCGGCGCTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCt                                                                                                                                               <  2:525475/150‑1 (MQ=255)
        gCCAGCCGCGTCTGCGGCGCTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTc                                                                                                                                              <  1:1148408/150‑1 (MQ=255)
         ccAGCCGCGTCTGCGGCGCTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCg                                                                                                                                             >  1:64844/1‑150 (MQ=255)
           aGCCGCGTCTGCGGCGCTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGGCCGCACCGGCCACCGCCGTGCCCAGCAATCCGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGc                                                                                                                                           >  1:1069602/1‑150 (MQ=255)
           aGCCGCGTCTGCGGCGCTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGACGGCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGc                                                                                                                                           >  1:1069343/1‑150 (MQ=255)
                cgTCTGCGGCGCTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGTGCCGAGCCCGGCTCGAc                                                                                                                                      <  2:345137/150‑1 (MQ=255)
                cgTCTGCGGCGCGGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTGGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGTGCCGAGCCCGGCTCGAc                                                                                                                                      <  2:390460/150‑1 (MQ=255)
                 gTCTGCGGCGCTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGAcc                                                                                                                                     <  1:82510/150‑1 (MQ=255)
                       ggcgCTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAg                                                                                                                               <  2:1381084/150‑1 (MQ=255)
                       ggcgCTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGAAGCGAAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAg                                                                                                                               <  1:1646571/150‑1 (MQ=255)
                        gcgcTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAgg                                                                                                                              <  2:830030/150‑1 (MQ=255)
                        gcgcTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAgg                                                                                                                              >  2:1678542/1‑150 (MQ=255)
                              aTCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTa                                                                                                                        >  1:176017/1‑150 (MQ=255)
                              aTCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGAACGCAACAAGTCAAGCCGGGCTCGGCACGACCGGTAAGGGTGTTa                                                                                                                        >  2:1590820/1‑150 (MQ=255)
                               tctcCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGAc                                                                                                                                      <  2:132179/135‑1 (MQ=255)
                               tctcCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGAc                                                                                                                                      >  1:132179/1‑135 (MQ=255)
                                 tcCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTAccc                                                                                                                     <  1:812327/150‑1 (MQ=255)
                                  ccAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCt                                                                                                                    <  1:414879/150‑1 (MQ=255)
                                        cgtcgCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCAt                                                                                                              <  2:339361/146‑1 (MQ=255)
                                            gCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTAAt                                                                                                          <  1:544090/150‑1 (MQ=255)
                                             ccGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATa                                                                                                         <  1:1120229/150‑1 (MQ=255)
                                             ccGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATa                                                                                                         >  2:1120229/1‑150 (MQ=255)
                                             ccGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATa                                                                                                         <  2:1457794/150‑1 (MQ=255)
                                             ccGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATa                                                                                                         >  2:461466/1‑150 (MQ=255)
                                                 cGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCa                                                                                                     >  2:3870/1‑150 (MQ=255)
                                                  gCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAg                                                                                                    >  1:41940/1‑150 (MQ=255)
                                                        tgGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGcc                                                                                                                                                    <  1:348301/96‑1 (MQ=255)
                                                        tgGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGcc                                                                                                                                                    >  2:348301/1‑96 (MQ=255)
                                                         ggCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACCACCGGTAAGGGTCCTTTCCTGCTCATTGATAGCCAggtcggtc                                                                                             <  1:51842/150‑1 (MQ=255)
                                                                 cAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAg                                                                                     <  1:826070/150‑1 (MQ=255)
                                                                  aGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGCCGGCGCCAgt                                                                                    >  2:702378/1‑150 (MQ=255)
                                                                   gggTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAgtg                                                                                   <  1:387956/150‑1 (MQ=255)
                                                                    ggTAATGCTGAACTGGCCGTCCTCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAgtgg                                                                                  <  1:721583/150‑1 (MQ=255)
                                                                    ggTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAgt                                                                                    >  2:1501767/1‑148 (MQ=255)
                                                                    ggTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAgt                                                                                    <  1:1501767/148‑1 (MQ=255)
                                                                        aTGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCa                                                                              <  2:1342464/150‑1 (MQ=255)
                                                                           cTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGtt                                                                           <  1:1678542/150‑1 (MQ=255)
                                                                            tGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTg                                                                          >  1:332997/1‑150 (MQ=255)
                                                                            tGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTg                                                                          <  1:3870/150‑1 (MQ=255)
                                                                                   gCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCgg                                                                   >  1:1708378/1‑150 (MQ=255)
                                                                                    ccGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGt                                                                  <  2:64844/150‑1 (MQ=255)
                                                                                     cGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCAAAAACGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTg                                                                 <  1:1590820/150‑1 (MQ=255)
                                                                                      gTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGc                                                                <  2:1421325/150‑1 (MQ=255)
                                                                                             ccGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACa                                                         <  2:41940/150‑1 (MQ=255)
                                                                                                  cACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGcc                                                    >  2:537161/1‑150 (MQ=255)
                                                                                                           gCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCga                                           <  2:1663371/150‑1 (MQ=255)
                                                                                                             ccAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAggtcggtc                                                                                             <  1:1171823/98‑1 (MQ=255)
                                                                                                             ccAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAggtcggtc                                                                                             >  2:1171823/1‑98 (MQ=255)
                                                                                                             ccAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCg                                                                    >  1:24876/1‑123 (MQ=255)
                                                                                                             ccAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCg                                                                    <  2:24876/123‑1 (MQ=255)
                                                                                                             ccAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCgaga                                         >  1:1080535/1‑150 (MQ=255)
                                                                                                              cAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGgt                                                                                                                            <  1:944787/66‑1 (MQ=255)
                                                                                                              cAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGgt                                                                                                                            >  2:944787/1‑66 (MQ=255)
                                                                                                              cAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGAc                                        >  2:1320493/1‑150 (MQ=255)
                                                                                                              cAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTAGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGAc                                        <  1:461466/150‑1 (MQ=255)
                                                                                                                    tACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCAACACCTGTAAGGGTTTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTAcc                                  <  1:1551973/150‑1 (MQ=255)
                                                                                                                                gcCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTc                                                                      <  1:1450399/102‑1 (MQ=255)
                                                                                                                                gcCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTc                                                                      >  2:1450399/1‑102 (MQ=255)
                                                                                                                                  cTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCgtcagtca                    >  2:380524/1‑150 (MQ=255)
                                                                                                                                    cGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAgg                  <  1:16254/150‑1 (MQ=255)
                                                                                                                                      cACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGt                <  2:281300/150‑1 (MQ=255)
                                                                                                                                       aCGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGCTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTg               >  2:1892481/1‑150 (MQ=255)
                                                                                                                                         ggAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGAc             >  1:1721991/1‑150 (MQ=255)
                                                                                                                                         cttaccggTCGTGCCGAGCCCGGCTCGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGAc             >  1:1334334/8‑150 (MQ=255)
                                                                                                                                           aaaCCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCCGTCAGGGTGACAt           >  1:891219/1‑150 (MQ=255)
                                                                                                                                              ccGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGACATCat        <  1:380524/150‑2 (MQ=255)
                                                                                                                                               cGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTCCCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGACATCAAg       <  1:482831/150‑1 (MQ=255)
                                                                                                                                                gTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCTCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGACATCAAgg      <  2:176017/150‑1 (MQ=255)
                                                                                                                                                gTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGcc                                             >  2:158491/1‑111 (MQ=255)
                                                                                                                                                gTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGcc                                             <  1:158491/111‑1 (MQ=255)
                                                                                                                                                 tCGTGCCGAGCCCGGCTCGACCGGTAAGGGTGTTACCCTGCTCATTGATAGc                                                                                                       <  1:977239/52‑1 (MQ=25)
                                                                                                                                                 tCGTGCCGAGCCCGGCTCGACCGGTAAGGGTGTTACCCTGCTCATTGATAGc                                                                                                       >  2:977239/1‑52 (MQ=21)
                                                                                                                                                    aGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGACATCAAGGtttt  >  2:200564/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GAGATATTGCCAGCCGCGTCTGCGGCGCTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGACATCAAGGTTTT  >  NC_002947/944681‑944978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑