BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	945,386	C→T	5.1%	G80D (GGC→GAC)	PP_0806 ←	surface adhesion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	945,386	0	C	T	5.1%	289.3 / 3.2	98	G80D (GGC→GAC)	PP_0806	surface adhesion protein
Reads supporting (aligned to +/- strand): ref base C (40/53); new base T (3/2); total (43/55)
Fisher's exact test for biased strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.55e-01

Only 100 of 103 total aligned reads displayed.

GGCTGAGCCCGGCAATCGCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTGGAGAAACCGGTACCTGC  >  NC_002947/945237‑945535

ggCTGAGCCCGGCAATCGCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAACCCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGc                                                                                                                                                       <  1:580162/150‑1 (MQ=255)

 gCTGAGCCCGGCAATCGCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGcc                                                                                                                                                      >  2:753132/1‑150 (MQ=255)

   tGAGCCCGGCAATCGCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCcga                                                                                                                                                    <  1:969789/150‑1 (MQ=255)

    gggCCCGGCAATCGCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCcgac                                                                                                                                                   <  1:644219/148‑1 (MQ=255)

      gCCCGGCAATCGCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCcgacga                                                                                                                                                 >  1:1798146/1‑150 (MQ=255)

       cccGGCAATCGCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCATCTTCGCCGACGaa                                                                                                                                                >  2:1075968/1‑150 (MQ=255)

         cGGCAATCGCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGa                                                                                                                                              <  2:1844692/150‑1 (MQ=255)

          ggCAATCGCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGAc                                                                                                                                             <  1:13538/150‑1 (MQ=255)

            cAATCGCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCAAATTGCGCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCa                                                                                                                                           <  1:1552929/150‑1 (MQ=255)

              aTCGCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAGCGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCata                                                                                                                                         <  1:1476081/150‑1 (MQ=255)

                cGCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATc                                                                                                                                       >  1:140596/1‑150 (MQ=255)

                cGCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATc                                                                                                                                       <  2:1685006/150‑1 (MQ=255)

                 gccgaaGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCg                                                                                                                                      <  2:410711/146‑1 (MQ=255)

                 gCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTGTTCGCCGACGAAGGCCATATCg                                                                                                                                      >  1:1031916/1‑150 (MQ=255)

                 gCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCg                                                                                                                                      >  1:481693/1‑150 (MQ=255)

                  cccAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGc                                                                                                                                     >  1:1796386/1‑150 (MQ=255)

                  cccAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGc                                                                                                                                     >  2:1284985/1‑150 (MQ=255)

                   ccAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGCCAATATcacc                                                                                                                                    <  2:472055/150‑4 (MQ=255)

                   ccAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCt                                                                                                                                    >  2:1128704/1‑150 (MQ=255)

                     aaGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGc                                                                                                                                  <  2:40955/150‑1 (MQ=255)

                        ttGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGa                                                                                                                               <  1:1284985/150‑1 (MQ=255)

                        ttGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGa                                                                                                                               <  2:1796386/150‑1 (MQ=255)

                         tggcatggcaCTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGAc                                                                                                                              >  2:283207/1‑150 (MQ=255)

                            catggcaCTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGAcccc                                                                                                                           >  2:1523517/1‑150 (MQ=255)

                             atggcaCTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCg                                                                                                                          >  1:843572/1‑150 (MQ=255)

                                gcaCTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCGACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGcc                                                                                                                       <  1:546516/150‑1 (MQ=255)

                                   ctgcctgcAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGGCCCCGGCCTgg                                                                                                                    <  1:1387679/150‑1 (MQ=255)

                                   ctgcctgcAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCACCGACCAAGACCATATCGCTGCCGACCCCGGCCTgg                                                                                                                    >  2:506922/1‑150 (MQ=255)

                                    tgcctgcAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGt                                                                                                                   >  1:1708221/1‑150 (MQ=255)

                                          cAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACgg                                                                                                             >  2:1026523/1‑150 (MQ=255)

                                                cGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGAc                                                                                                                                             >  2:607415/1‑112 (MQ=255)

                                                cGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGAc                                                                                                                                             <  1:607415/112‑1 (MQ=255)

                                                 gATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCgaagaa                                                                                                      <  2:898286/150‑1 (MQ=255)

                                                 gATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCgaagaa                                                                                                      <  1:1861666/150‑1 (MQ=255)

                                                  aTGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGaagaag                                                                                                     <  2:481693/150‑1 (MQ=255)

                                                    gCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGtt                                                                                                   <  1:1878249/150‑1 (MQ=255)

                                                       tGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGTCGGCGAAGAcggca                                                                                                                                         >  1:1570474/1‑105 (MQ=255)

                                                          gACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGAt                                                                                             <  2:89400/150‑1 (MQ=255)

                                                            ccAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATgg                                                                                           <  2:1487103/150‑1 (MQ=255)

                                                            ccAGTTCCTCCAGCGAGGCCACTTCTTCGAAGGTTAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATgg                                                                                           <  1:1142978/150‑1 (MQ=255)

                                                               gTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGa                                                                                        >  1:538757/1‑150 (MQ=255)

                                                                ttCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGAt                                                                                       <  1:753132/150‑1 (MQ=255)

                                                                    tCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCttt                                                                                   <  1:197297/150‑1 (MQ=255)

                                                                      cAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTc                                                                                 <  2:843572/150‑1 (MQ=255)

                                                                       aGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTcc                                                                                <  1:283207/150‑1 (MQ=255)

                                                                        gCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTccc                                                                               >  2:1045129/1‑150 (MQ=255)

                                                                          gAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTcccca                                                                             <  2:343587/150‑2 (MQ=255)

                                                                             gCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTg                                                                                                                     >  2:1317703/1‑107 (MQ=255)

                                                                             gCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTg                                                                                                                     <  1:1317703/107‑1 (MQ=255)

                                                                               cACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACtt                                                                        >  2:1861196/1‑150 (MQ=255)

                                                                               cACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACtt                                                                        >  2:843464/1‑150 (MQ=255)

                                                                                aCTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTc                                                                       >  1:561437/1‑150 (MQ=255)

                                                                                aCTTCTTCAAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTACCAGACGAAGACCATATCGCTGCCGTCCCCGGCCTTTTCCTCGGCGAAGAAGTTACAGATGGTGATCCTTTCCCCCGACTTc                                                                       >  2:687352/1‑150 (MQ=255)

                                                                                  ttcttcGAAGGTGAAAGCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAg                                                                     >  2:347910/1‑150 (MQ=255)

                                                                                   tcttcGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAgg                                                                    <  2:580407/150‑1 (MQ=255)

                                                                                   tcttcGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAgg                                                                    <  1:305114/150‑1 (MQ=255)

                                                                                   tcttcGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAgg                                                                    <  1:1861196/150‑1 (MQ=255)

                                                                                   tcttcGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCATCTTCGCCGACGAAGAACACATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAgg                                                                    <  1:1075968/150‑1 (MQ=255)

                                                                                     ttcGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAgg                                                                    >  2:466205/1‑148 (MQ=255)

                                                                                     ttcGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAgg                                                                    <  1:466205/148‑1 (MQ=255)

                                                                                     ttcGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGt                                                                  >  2:302546/1‑150 (MQ=255)

                                                                                        gAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGc                                                                                                                                  >  1:1268183/1‑83 (MQ=255)

                                                                                        gAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGc                                                                                                                                  <  2:1268183/83‑1 (MQ=255)

                                                                                        gAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATAAACGTTGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGAc                                                               >  1:118380/1‑150 (MQ=255)

                                                                                            gTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATc                                                           <  1:1128704/150‑1 (MQ=255)

                                                                                                aaCCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGt                                                       >  2:625646/1‑150 (MQ=255)

                                                                                                   cGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCt                                                    <  2:162495/150‑1 (MQ=255)

                                                                                                             tGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTg                                          >  1:1550555/1‑150 (MQ=255)

                                                                                                                 gCATCGTACTGGGCATACCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCAACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGAGCAGGTCCTGCCCGCGCTGGGTg                                      >  2:195215/1‑150 (MQ=255)

                                                                                                                    tCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGAcc                                   <  1:1408762/150‑1 (MQ=255)

                                                                                                                       tACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCa                                                                                                                <  2:1385744/70‑1 (MQ=255)

                                                                                                                       tACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCa                                                                                                                >  1:1385744/1‑70 (MQ=255)

                                                                                                                       tACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTg                                >  2:1272710/1‑150 (MQ=255)

                                                                                                                       tACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTg                                <  1:996145/150‑1 (MQ=255)

                                                                                                                         cTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGc                              >  1:1491813/1‑150 (MQ=255)

                                                                                                                                   cacaGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAgg                                                                    >  2:602641/1‑102 (MQ=255)

                                                                                                                                   cacaGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCt                    <  2:1798146/150‑1 (MQ=255)

                                                                                                                                   cacaGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCt                    <  2:140596/150‑1 (MQ=255)

                                                                                                                                   cacaGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCt                    >  1:490925/1‑150 (MQ=255)

                                                                                                                                   cacaGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCt                    <  1:1252879/150‑1 (MQ=255)

                                                                                                                                   cacaGGGTGCCGTCTTCGCCGACAAAAACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAgg                                                                    <  1:602641/102‑1 (MQ=255)

                                                                                                                                    acaGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTg                   >  2:64492/1‑150 (MQ=255)

                                                                                                                                     caGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCAAATCGTTGGTGACCGTGGCCACTTTGTCTgg                  <  1:1484133/150‑1 (MQ=255)

                                                                                                                                      aGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTGga                 <  2:1708221/150‑1 (MQ=255)

                                                                                                                                        ggTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTGgaga               <  2:118380/150‑1 (MQ=255)

                                                                                                                                          tatggTCTTCGTCGGCGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTCCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTg                   >  1:1364731/5‑144 (MQ=255)

                                                                                                                                          tatggTCTTCGTCGGCGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTCCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTg                   <  2:1364731/140‑1 (MQ=255)

                                                                                                                                          tGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTGGAGaaa             >  1:232190/1‑150 (MQ=255)

                                                                                                                                           gCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTGGAGAAAc            <  1:1045129/150‑1 (MQ=255)

                                                                                                                                           gCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTGGAGAAAc            <  2:490925/150‑1 (MQ=255)

                                                                                                                                            ccGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTg                                <  2:478092/129‑1 (MQ=255)

                                                                                                                                            ccGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTg                                >  1:478092/1‑129 (MQ=255)

                                                                                                                                              gTCTTCGTCGGCGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTGGAGAAACCgg         >  2:631348/1‑150 (MQ=255)

                                                                                                                                                cTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTGGAGAAACCGGTa       <  2:1550555/150‑1 (MQ=255)

                                                                                                                                                 ttCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTGGAGAAACCGGTAc      <  1:800439/150‑1 (MQ=255)

                                                                                                                                                  tCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTGGAGAAACCGGTAcc     <  2:232190/150‑1 (MQ=255)

                                                                                                                                                   cGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGAACGTTGCCACTTTGTCTGGAGAAACCGGTACCt    <  1:347910/150‑1 (MQ=255)

                                                                                                                                                     ccGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAggg                                                                   <  1:1318456/85‑1 (MQ=255)

                                                                                                                                                     ccGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAggg                                                                   >  2:1318456/1‑85 (MQ=255)

                                                                                                                                                     ccGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTGGAGAAACCGGTACCTGc  <  1:1026523/150‑1 (MQ=255)

GGCTGAGCCCGGCAATCGCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTGGAGAAACCGGTACCTGC  >  NC_002947/945237‑945535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`