BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	945,389	A→G	5.1%	V79A (GTC→GCC)	PP_0806 ←	surface adhesion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	945,389	0	A	G	5.1%	286.0 / 3.6	98	V79A (GTC→GCC)	PP_0806	surface adhesion protein
Reads supporting (aligned to +/- strand): ref base A (40/53); new base G (3/2); total (43/55)
Fisher's exact test for biased strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.25e-01

Only 100 of 101 total aligned reads displayed.

TGAGCCCGGCAATCGCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTGGAGAAACCGGTACCTGC  >  NC_002947/945240‑945535

tGAGCCCGGCAATCGCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCcga                                                                                                                                                    <  1:969789/150‑1 (MQ=255)

 gggCCCGGCAATCGCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCcgac                                                                                                                                                   <  1:644219/148‑1 (MQ=255)

   gCCCGGCAATCGCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCcgacga                                                                                                                                                 >  1:1798146/1‑150 (MQ=255)

    cccGGCAATCGCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCATCTTCGCCGACGaa                                                                                                                                                >  2:1075968/1‑150 (MQ=255)

      cGGCAATCGCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGa                                                                                                                                              >  1:343587/1‑150 (MQ=255)

      cGGCAATCGCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGa                                                                                                                                              <  2:1844692/150‑1 (MQ=255)

       ggCAATCGCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGAc                                                                                                                                             <  1:13538/150‑1 (MQ=255)

         cAATCGCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCAAATTGCGCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCa                                                                                                                                           <  1:1552929/150‑1 (MQ=255)

           aTCGCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAGCGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCata                                                                                                                                         <  1:1476081/150‑1 (MQ=255)

             cGCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATc                                                                                                                                       >  1:140596/1‑150 (MQ=255)

             cGCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATc                                                                                                                                       <  2:1685006/150‑1 (MQ=255)

              gccgaaGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCg                                                                                                                                      <  2:410711/146‑1 (MQ=255)

              gCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTGTTCGCCGACGAAGGCCATATCg                                                                                                                                      >  1:1031916/1‑150 (MQ=255)

              gCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCg                                                                                                                                      >  1:481693/1‑150 (MQ=255)

               cccAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGc                                                                                                                                     >  2:1284985/1‑150 (MQ=255)

               cccAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGc                                                                                                                                     >  1:1796386/1‑150 (MQ=255)

                ccAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGCCAATATcacc                                                                                                                                    <  2:472055/150‑4 (MQ=255)

                ccAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCt                                                                                                                                    >  2:1128704/1‑150 (MQ=255)

                  aaGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGc                                                                                                                                  <  2:40955/150‑1 (MQ=255)

                     ttGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGa                                                                                                                               <  1:1284985/150‑1 (MQ=255)

                     ttGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGa                                                                                                                               <  2:1796386/150‑1 (MQ=255)

                      tggcatggcaCTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGAc                                                                                                                              >  2:283207/1‑150 (MQ=255)

                         catggcaCTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGAcccc                                                                                                                           >  2:1523517/1‑150 (MQ=255)

                          atggcaCTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCg                                                                                                                          >  1:843572/1‑150 (MQ=255)

                             gcaCTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCGACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGcc                                                                                                                       <  1:546516/150‑1 (MQ=255)

                                ctgcctgcAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGGCCCCGGCCTgg                                                                                                                    <  1:1387679/150‑1 (MQ=255)

                                ctgcctgcAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCACCGACCAAGACCATATCGCTGCCGACCCCGGCCTgg                                                                                                                    >  2:506922/1‑150 (MQ=255)

                                 tgcctgcAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGt                                                                                                                   >  1:1708221/1‑150 (MQ=255)

                                       cAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACgg                                                                                                             >  2:1026523/1‑150 (MQ=255)

                                             cGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGAc                                                                                                                                             >  2:607415/1‑112 (MQ=255)

                                             cGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGAc                                                                                                                                             <  1:607415/112‑1 (MQ=255)

                                              gATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCgaagaa                                                                                                      <  2:898286/150‑1 (MQ=255)

                                              gATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCgaagaa                                                                                                      <  1:1861666/150‑1 (MQ=255)

                                               aTGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGaagaag                                                                                                     <  2:481693/150‑1 (MQ=255)

                                                 gCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGtt                                                                                                   <  1:1878249/150‑1 (MQ=255)

                                                    tGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGTCGGCGAAGAcggca                                                                                                                                         >  1:1570474/1‑105 (MQ=255)

                                                    tGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGTCGGCGAAGAcggca                                                                                                                                         <  2:1570474/109‑5 (MQ=255)

                                                       gACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGAt                                                                                             <  2:89400/150‑1 (MQ=255)

                                                         ccAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATgg                                                                                           <  2:1487103/150‑1 (MQ=255)

                                                         ccAGTTCCTCCAGCGAGGCCACTTCTTCGAAGGTTAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATgg                                                                                           <  1:1142978/150‑1 (MQ=255)

                                                            gTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGa                                                                                        >  1:538757/1‑150 (MQ=255)

                                                             ttCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGAt                                                                                       <  1:753132/150‑1 (MQ=255)

                                                                 tCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCttt                                                                                   <  1:197297/150‑1 (MQ=255)

                                                                   cAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTc                                                                                 <  2:843572/150‑1 (MQ=255)

                                                                    aGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTcc                                                                                <  1:283207/150‑1 (MQ=255)

                                                                     gCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTccc                                                                               >  2:1045129/1‑150 (MQ=255)

                                                                       gAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTcccca                                                                             <  2:343587/150‑2 (MQ=255)

                                                                          gCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTg                                                                                                                     <  1:1317703/107‑1 (MQ=255)

                                                                          gCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTg                                                                                                                     >  2:1317703/1‑107 (MQ=255)

                                                                            cACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACtt                                                                        >  2:843464/1‑150 (MQ=255)

                                                                            cACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACtt                                                                        >  2:1861196/1‑150 (MQ=255)

                                                                             aCTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTc                                                                       >  1:561437/1‑150 (MQ=255)

                                                                             aCTTCTTCAAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTACCAGACGAAGACCATATCGCTGCCGTCCCCGGCCTTTTCCTCGGCGAAGAAGTTACAGATGGTGATCCTTTCCCCCGACTTc                                                                       >  2:687352/1‑150 (MQ=255)

                                                                               ttcttcGAAGGTGAAAGCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAg                                                                     >  2:347910/1‑150 (MQ=255)

                                                                                tcttcGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAgg                                                                    <  1:1861196/150‑1 (MQ=255)

                                                                                tcttcGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAgg                                                                    <  2:580407/150‑1 (MQ=255)

                                                                                tcttcGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAgg                                                                    <  1:305114/150‑1 (MQ=255)

                                                                                tcttcGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCATCTTCGCCGACGAAGAACACATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAgg                                                                    <  1:1075968/150‑1 (MQ=255)

                                                                                  ttcGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAgg                                                                    <  1:466205/148‑1 (MQ=255)

                                                                                  ttcGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAgg                                                                    >  2:466205/1‑148 (MQ=255)

                                                                                  ttcGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGt                                                                  >  2:302546/1‑150 (MQ=255)

                                                                                     gAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGc                                                                                                                                  <  2:1268183/83‑1 (MQ=255)

                                                                                     gAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGc                                                                                                                                  >  1:1268183/1‑83 (MQ=255)

                                                                                     gAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATAAACGTTGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGAc                                                               >  1:118380/1‑150 (MQ=255)

                                                                                         gTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATc                                                           <  1:1128704/150‑1 (MQ=255)

                                                                                             aaCCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGt                                                       >  2:625646/1‑150 (MQ=255)

                                                                                                cGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCt                                                    <  2:162495/150‑1 (MQ=255)

                                                                                                          tGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTg                                          >  1:1550555/1‑150 (MQ=255)

                                                                                                              gCATCGTACTGGGCATACCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCAACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGAGCAGGTCCTGCCCGCGCTGGGTg                                      >  2:195215/1‑150 (MQ=255)

                                                                                                                 tCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGAcc                                   <  1:1408762/150‑1 (MQ=255)

                                                                                                                    tACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCa                                                                                                                >  1:1385744/1‑70 (MQ=255)

                                                                                                                    tACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCa                                                                                                                <  2:1385744/70‑1 (MQ=255)

                                                                                                                    tACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTg                                >  2:1272710/1‑150 (MQ=255)

                                                                                                                    tACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTg                                <  1:996145/150‑1 (MQ=255)

                                                                                                                      cTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGc                              >  1:1491813/1‑150 (MQ=255)

                                                                                                                                cacaGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAgg                                                                    >  2:602641/1‑102 (MQ=255)

                                                                                                                                cacaGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCt                    <  2:140596/150‑1 (MQ=255)

                                                                                                                                cacaGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCt                    <  2:1798146/150‑1 (MQ=255)

                                                                                                                                cacaGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCt                    <  1:1252879/150‑1 (MQ=255)

                                                                                                                                cacaGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCt                    >  1:490925/1‑150 (MQ=255)

                                                                                                                                cacaGGGTGCCGTCTTCGCCGACAAAAACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAgg                                                                    <  1:602641/102‑1 (MQ=255)

                                                                                                                                 acaGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTg                   >  2:64492/1‑150 (MQ=255)

                                                                                                                                  caGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCAAATCGTTGGTGACCGTGGCCACTTTGTCTgg                  <  1:1484133/150‑1 (MQ=255)

                                                                                                                                   aGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTGga                 <  2:1708221/150‑1 (MQ=255)

                                                                                                                                     ggTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTGgaga               <  2:118380/150‑1 (MQ=255)

                                                                                                                                       tatggTCTTCGTCGGCGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTCCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTg                   >  1:1364731/5‑144 (MQ=255)

                                                                                                                                       tatggTCTTCGTCGGCGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTCCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTg                   <  2:1364731/140‑1 (MQ=255)

                                                                                                                                       tGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTGGAGaaa             >  1:232190/1‑150 (MQ=255)

                                                                                                                                        gCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTGGAGAAAc            <  2:490925/150‑1 (MQ=255)

                                                                                                                                        gCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTGGAGAAAc            <  1:1045129/150‑1 (MQ=255)

                                                                                                                                         ccGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTg                                <  2:478092/129‑1 (MQ=255)

                                                                                                                                         ccGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTg                                >  1:478092/1‑129 (MQ=255)

                                                                                                                                           gTCTTCGTCGGCGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTGGAGAAACCgg         >  2:631348/1‑150 (MQ=255)

                                                                                                                                             cTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTGGAGAAACCGGTa       <  2:1550555/150‑1 (MQ=255)

                                                                                                                                              ttCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTGGAGAAACCGGTAc      <  1:800439/150‑1 (MQ=255)

                                                                                                                                               tCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTGGAGAAACCGGTAcc     <  2:232190/150‑1 (MQ=255)

                                                                                                                                                cGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGAACGTTGCCACTTTGTCTGGAGAAACCGGTACCt    <  1:347910/150‑1 (MQ=255)

                                                                                                                                                  ccGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAggg                                                                   <  1:1318456/85‑1 (MQ=255)

                                                                                                                                                  ccGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAggg                                                                   >  2:1318456/1‑85 (MQ=255)

                                                                                                                                                  ccGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTGGAGAAACCGGTACCTGc  <  1:1026523/150‑1 (MQ=255)

TGAGCCCGGCAATCGCCCAAGTTGGCATGGCACTGCCTGCAGTACCGATGCCTGCGACCAGTTCGTCCAGCGAGGCCACTTCTTCGAAGGTGAAACCGGTGAACGCTGCCGCATCGTACTGGGCATGCCACAGGGTGCCGTCTTCGCCGACGAAGACCATATCGCTGCCGACCCCGGCCTGGTCCACGGCGAAGAAGTTACCGATGGTGATCTTTTCCCCCGACTTCAGGGTGACGATCAGGTCCTGCCCGCGCTGGGTGACCGTGGCCACTTTGTCTGGAGAAACCGGTACCTGC  >  NC_002947/945240‑945535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`