Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 160,310 C→T 6.5% E27K (GAG→AAG)  PP_0149 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947160,3100CT 6.5% 217.3 / 4.8 78E27K (GAG→AAG) PP_0149hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (38/34);  new base T (2/3);  total (40/38)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.85e-01

CGTCGGCAGGCATGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGT  >  NC_002947/160163‑160459
                                                                                                                                                    |                                                                                                                                                     
cGTCGGCAGGCATGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCaa                                                                                                                                                     <  1:700996/150‑1 (MQ=255)
    ggcaggcaTGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCCACGCAAGCTg                                                                                                                                                 >  1:1021183/1‑150 (MQ=255)
         gcaTGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACA‑CCTGCAGCTTGAGCTGCAggc                                                                                                                                            <  2:1174382/150‑2 (MQ=255)
           aTGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCg                                                                                                                                          <  1:1650680/150‑1 (MQ=255)
                gtCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGCCGACCTGCAGCTTGAGCTGCAggcgtccgg                                                                                                                                      <  1:757413/150‑8 (MQ=255)
                gtCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCaa                                                                                                                                     <  1:1845034/150‑1 (MQ=255)
                     tCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCAAGATCAAGGCCAAATACTGGCCTCGTCGCGCAGTGCGTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAggcgg                                                                                                                                >  1:1673144/1‑150 (MQ=255)
                           tGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGc                                                                                                                          <  2:215596/150‑1 (MQ=255)
                            gACCCGCATTCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGcc                                                                                                                         <  1:1515355/150‑1 (MQ=255)
                              cccGCACGCGGCGGTCCCGCTGTTGCTTGAAGGGCGGTGGGCGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCCGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGa                                                                                                                       <  1:448786/150‑1 (MQ=255)
                               ccGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGag                                                                                                                      >  2:32790/1‑150 (MQ=255)
                               ccGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGag                                                                                                                      >  2:249742/1‑150 (MQ=255)
                               ccGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGag                                                                                                                      >  2:1377590/1‑150 (MQ=255)
                                            tCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGgcg                                                                                                         >  2:1719935/1‑150 (MQ=255)
                                              cAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGgcgcc                                                                                                       >  2:1398005/1‑149 (MQ=255)
                                                   ggtGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCaa                                                                                                  <  2:1383971/148‑1 (MQ=255)
                                                    ttGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAag                                                                                                 <  1:1306762/150‑1 (MQ=255)
                                                       cTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAagcag                                                                                              >  1:620831/1‑150 (MQ=255)
                                                        ttGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGGCG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAagcagc                                                                                             >  2:199399/1‑150 (MQ=255)
                                                        ttGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTGTGTTGTGGGACGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAagcagc                                                                                             >  2:985916/1‑150 (MQ=255)
                                                                  gTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAggg                                                                                                           >  2:1477513/1‑126 (MQ=255)
                                                                  gTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAggg                                                                                                           <  1:1477513/126‑1 (MQ=255)
                                                                    ggCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGcacgcacg                                                                                 <  1:1377590/150‑1 (MQ=255)
                                                                     gCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGc                                                                                                                                                   <  1:1661163/83‑1 (MQ=255)
                                                                     gCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGc                                                                                                                                                   >  2:1661163/1‑83 (MQ=255)
                                                                          ttGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTTGAGCTGCAggcgt                                                                                                                                          <  2:1196219/87‑4 (MQ=39)
                                                                          ttGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTTGAGCTGCAggcgt                                                                                                                                          >  1:1196219/1‑84 (MQ=39)
                                                                              ccATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAg                                                                       >  2:335842/1‑150 (MQ=255)
                                                                              ccATGGCCTCGTCGAGCAGGGCTGGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAg                                                                       >  2:942803/1‑150 (MQ=255)
                                                                               cATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGTAGCCAGGCGCGCACGCGCTTcagggt                                                                      >  1:123391/1‑146 (MQ=255)
                                                                                 tGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGc                                                                    <  1:243805/150‑1 (MQ=255)
                                                                                  ggCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGa                                                                                                                       >  2:1368624/1‑98 (MQ=255)
                                                                                  ggCCTCGTCGAGCAGGCCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGa                                                                                                                       <  1:1368624/98‑1 (MQ=255)
                                                                                   gCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGa                                                                  <  1:781894/150‑1 (MQ=255)
                                                                                     cTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGt                                                                >  2:770339/1‑150 (MQ=255)
                                                                                     cTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGt                                                                >  1:1859569/1‑150 (MQ=255)
                                                                                      tcgtcgAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGCGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCAGAGCAGCGGGAGGGCGCAGCCAAGCAGCCATGCACGCACGCGCTTCCCAGTGCGAGTa                                                               >  2:1261148/1‑150 (MQ=255)
                                                                                      tcgtcgAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTa                                                               >  1:931152/1‑150 (MQ=255)
                                                                                      tcgtcgAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTa                                                               >  1:482867/1‑150 (MQ=255)
                                                                                        gtcgAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATg                                                             <  1:1675723/150‑1 (MQ=255)
                                                                                        gtcgAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATg                                                             >  2:1524643/1‑150 (MQ=255)
                                                                                         tcgAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATgg                                                            <  1:14939/150‑1 (MQ=255)
                                                                                          cgAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGc                                                           <  2:482867/150‑1 (MQ=255)
                                                                                             gCAGGGCTTCGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTc                                                        >  2:514658/1‑150 (MQ=255)
                                                                                               aGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAg                                                      >  1:1246242/1‑150 (MQ=255)
                                                                                                   cTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGTCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCaa                                                  >  2:1145350/1‑150 (MQ=255)
                                                                                                    ttGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGc                                                     <  1:704840/146‑1 (MQ=255)
                                                                                                    ttGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGc                                                     >  2:704840/1‑146 (MQ=255)
                                                                                                     tGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATa                                                <  1:39248/150‑1 (MQ=255)
                                                                                                       ggAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATAcc                                              <  2:1063540/150‑1 (MQ=255)
                                                                                                        gAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCt                                             >  2:567149/1‑150 (MQ=255)
                                                                                                        gAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCt                                             <  1:1236748/150‑1 (MQ=255)
                                                                                                         aGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTg                                            <  2:615814/150‑1 (MQ=255)
                                                                                                          ggCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTgg                                           <  1:1524643/150‑1 (MQ=255)
                                                                                                           gCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGACACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGt                                          >  2:1322716/1‑150 (MQ=255)
                                                                                                                 gTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTgg                                    <  2:1601306/150‑1 (MQ=255)
                                                                                                                  tttGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGAATGGCTTCAGCCAATACCTGGTCGCTGgc                                   >  2:824669/1‑150 (MQ=255)
                                                                                                                     gttgtGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGcgcc                                >  1:47481/1‑150 (MQ=255)
                                                                                                                       tgtgGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCAGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGcgccgc                              <  2:57455/150‑1 (MQ=255)
                                                                                                                         tgGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCcgcgc                            <  1:53035/150‑1 (MQ=255)
                                                                                                                          gggTCGAGTCCGGACG‑CCTGCAGCTCACGCTGCAGGTCGGCCAAGGCGCGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCcgcgcg                           <  2:1673144/150‑1 (MQ=255)
                                                                                                                             tCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCCAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGTCACGCACGCGCTTCACAGTGCGAGTCTGGCTTCAGCCAATGCCTGGTCGCCGGCCCCGCGCGCtt                        >  1:596185/1‑150 (MQ=255)
                                                                                                                             tCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCtt                        <  1:567149/150‑1 (MQ=255)
                                                                                                                              cGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTc                       <  1:32790/150‑1 (MQ=255)
                                                                                                                                aGGCCGGACG‑CCTGCAGCTGACGCGGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCg                     <  1:96656/150‑1 (MQ=255)
                                                                                                                                    cttaCG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCAAGGCAAGCACGCGCTTAACAGTGCGAGAATGGCAGCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTGCCGGTAc                 >  1:746762/4‑150 (MQ=255)
                                                                                                                                      gACG‑CCTGCAGCTCACGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGACACAGGCAATACGTGTTCGCTGGCGCGTCGGGTTTCGGGTattg               <  2:1261643/150‑4 (MQ=255)
                                                                                                                                      gACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTActc               <  2:445010/150‑1 (MQ=255)
                                                                                                                                       aCG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCcgcgc                            >  2:433555/1‑136 (MQ=255)
                                                                                                                                       aCG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCcgcgc                            <  1:433555/136‑1 (MQ=255)
                                                                                                                                        cgaccTGCAGCTTGAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTActcg              >  1:1397546/4‑150 (MQ=255)
                                                                                                                                         g‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTActcgct            <  2:931152/150‑1 (MQ=255)
                                                                                                                                            cTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGcc                                                                                                    <  1:1735613/60‑1 (MQ=255)
                                                                                                                                            cTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGcc                                                                                                    >  2:1735613/1‑60 (MQ=255)
                                                                                                                                            cTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTActcgctcg          >  1:1638296/1‑150 (MQ=255)
                                                                                                                                             tGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACtcgctcgc         >  1:1250664/1‑150 (MQ=255)
                                                                                                                                              gCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTcgcg        >  1:877995/1‑150 (MQ=255)
                                                                                                                                                   tCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAggg   >  1:1345651/1‑150 (MQ=255)
                                                                                                                                                    cAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGt  >  2:1138218/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGTCGGCAGGCATGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACG‑CCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGT  >  NC_002947/160163‑160459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑