Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,134,095 A→T 5.3% intergenic (+34/+44) PP_0995 → / ← gltS membrane protein/glutamate:sodium symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,134,0950AT 5.3% 234.2 / 2.3 77intergenic (+34/+44)PP_0995/gltSmembrane protein/glutamate:sodium symporter
Reads supporting (aligned to +/- strand):  ref base A (41/31);  new base T (2/2);  total (43/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

CCTGCATTGCCCGGAAATGGGCGTGCCGTTCTGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGATGAAGAACGCCCCCACCATCGGCACCACCAGGAAGGCGAG  >  NC_002947/1133950‑1134243
                                                                                                                                                 |                                                                                                                                                    
cctgcATTGCCCGGAAATGGGCGTGCCGTTCTGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGtt                                                                                                                                                  <  2:625099/150‑1 (MQ=255)
  tgcATTGCCCGGAAATGGGCGTGCCGTTCTGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCt                                                                                                                                                >  2:314767/1‑150 (MQ=255)
    cATTGCCGGGAAATGGGCGTGCCGTTCTGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGTCGTTCTGa                                                                                                                                              <  1:446286/150‑1 (MQ=255)
    cATTGCCCGGAAATGGGCGTGCCGTTCTGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGa                                                                                                                                              <  1:853708/150‑1 (MQ=255)
     ggtGCCCGGAAATGGGCGTGCCGTTCTAGGGTGTGCGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGTCGTTCTGag                                                                                                                                             <  2:1259309/148‑2 (MQ=255)
        gCCCGGAAATGGGCGTGCCGTTCGGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGAttgc                                                                                                                                          <  1:96771/150‑1 (MQ=255)
          ccGGAAATGGGCGTGCCGTTCTGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGa                                                                                                                                              <  2:1883503/144‑1 (MQ=255)
          ccGGAAATGGGCGTGCCGTTCTGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGa                                                                                                                                              >  1:1883503/1‑144 (MQ=255)
             gAAATGGGCGTGCCGTTCTGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGcc                                                                                                                                     <  1:314767/150‑1 (MQ=255)
                 tGGGCGTGCAGTTCTGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGAttg                                                                                                                                           <  2:1777376/140‑1 (MQ=255)
                 tGGGCGTGCAGTTCTGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGAttg                                                                                                                                           >  1:1777376/1‑140 (MQ=255)
                        gCCGTTCTGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTcaca                                                                                                                          <  2:1351590/150‑1 (MQ=255)
                               tGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCATAGGTTCGt                                                                                                                   <  1:731476/150‑1 (MQ=255)
                               tGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGt                                                                                                                   <  1:1064852/150‑1 (MQ=255)
                               tGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGt                                                                                                                   <  1:509632/150‑1 (MQ=255)
                                 gggTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGc                                                                                                                                <  2:726929/135‑1 (MQ=255)
                                 gggTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGc                                                                                                                                >  1:726929/1‑135 (MQ=255)
                                 gggTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGa                                                                                                                 <  1:226156/150‑1 (MQ=255)
                                    tgtgtgGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGAttgcttgc                                                                                                                                      >  1:1847636/1‑126 (MQ=255)
                                    tgtgtgGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGAttgcttgc                                                                                                                                      <  2:1847636/126‑1 (MQ=255)
                                     gtgtgGTATGTCCTCGGCATGCTCGTGCCAACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTc                                                                                                             <  1:528515/150‑1 (MQ=255)
                                       gtgGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGc                                                                                                           >  2:121519/1‑150 (MQ=255)
                                        tgGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCt                                                                                                          >  2:289495/1‑150 (MQ=255)
                                          gTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTa                                                                                                        >  2:1315196/1‑150 (MQ=255)
                                            aTGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAAc                                                                                                      <  1:997240/150‑1 (MQ=255)
                                            aTGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAAc                                                                                                      <  2:249860/150‑1 (MQ=255)
                                               tCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCg                                                                                                   >  1:552070/1‑150 (MQ=255)
                                                  tCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGcaa                                                                                                >  2:904494/1‑150 (MQ=255)
                                                         gCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTTTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTTGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCa                                                                                         <  1:142640/150‑1 (MQ=255)
                                                         gCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCa                                                                                         >  1:897706/1‑150 (MQ=255)
                                                           tCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGt                                                                                                                   >  2:323023/1‑122 (MQ=255)
                                                           tCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGt                                                                                                                   <  1:323023/122‑1 (MQ=255)
                                                           tCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAg                                                                                       <  2:566564/150‑1 (MQ=255)
                                                           tCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAg                                                                                       <  1:1324712/150‑1 (MQ=255)
                                                            cGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCACAAAACCAAgg                                                                                      <  2:552070/150‑1 (MQ=255)
                                                                ccGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAAt                                                                                  <  1:1315196/150‑1 (MQ=255)
                                                                ccGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAAt                                                                                  >  1:55898/1‑150 (MQ=255)
                                                                ccGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCGTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAAt                                                                                  >  2:773896/1‑150 (MQ=255)
                                                                  gACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGAAATGc                                                                                >  2:1241068/1‑150 (MQ=255)
                                                                   aCGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGtt                                                                                                                      >  2:1416563/1‑111 (MQ=255)
                                                                   aCGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGtt                                                                                                                      <  1:1416563/111‑1 (MQ=255)
                                                                     ggTACTCGGTGCCTGGTGGGGCACCCGCCTGCTGCGTCGGTAGGCTGTACAGACCGGCCCCACTCTACTCACAACGACGTTCTGATTGCTTGCCCCCGCTCCACAGGTTCGTTACCTCGCTTAACCCGCAGCACACCAAGGCAATGCCAg                                                                             >  2:266864/1‑150 (MQ=255)
                                                                       tACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCATGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGt                                                                           >  2:694028/1‑150 (MQ=255)
                                                                         cTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTACGTGACCTCGCTTAAcc                                                                                                     >  2:567344/1‑122 (MQ=255)
                                                                         cTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTACGTGACCTCGCTTAAcc                                                                                                     <  1:567344/122‑1 (MQ=255)
                                                                          tCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACaaa                                                                                            <  2:395061/130‑1 (MQ=255)
                                                                          tCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACaaa                                                                                            >  1:395061/1‑130 (MQ=255)
                                                                                ccTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACATCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGa                                                                  >  1:109126/1‑150 (MQ=255)
                                                                                 cTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGAt                                                                 >  1:860189/1‑150 (MQ=255)
                                                                                  tggtggGGCCCCCGCATGCTGCGTTGGTGGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAACCCAAGGCAATGCCAGGTACAGTTTGATc                                                                >  1:466105/1‑150 (MQ=255)
                                                                                  tggtggGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATc                                                                >  2:705786/1‑150 (MQ=255)
                                                                                   ggtggGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCa                                                               >  2:257541/1‑150 (MQ=255)
                                                                                         gCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGa                                                         <  2:1292255/150‑1 (MQ=255)
                                                                                             ccGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACAtt                                                     >  2:1674222/1‑150 (MQ=255)
                                                                                                     tgcGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGc                                                                                                           >  1:1901930/1‑88 (MQ=255)
                                                                                                     tgcGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGc                                                                                                           <  2:1901930/88‑1 (MQ=255)
                                                                                                       cGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCg                                           >  1:1751918/1‑150 (MQ=255)
                                                                                                        gTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGACATGTTTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGa                                          <  1:496196/150‑1 (MQ=255)
                                                                                                        gTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGa                                          >  1:487724/1‑150 (MQ=255)
                                                                                                               ggCTGTACCGGCAGTCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGATgaagaa                                   >  2:559956/1‑150 (MQ=255)
                                                                                                                 cTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCTATGAAGAACg                                 >  2:334535/1‑150 (MQ=255)
                                                                                                                  tGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGATGAAGAgcgc                                >  2:1115527/1‑150 (MQ=255)
                                                                                                                      ccGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGATGAAGAACGccccc                            >  2:127607/1‑150 (MQ=255)
                                                                                                                         gCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATtga                                                   >  1:110560/1‑124 (MQ=255)
                                                                                                                         gCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATtga                                                   <  2:110560/124‑1 (MQ=255)
                                                                                                                            ggCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGATGAAGAACGCCCCCACCATc                      <  2:1916128/150‑1 (MQ=255)
                                                                                                                               cccACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTAATTACCATGAAACTGAGGATTGCGATGAAGAACGCCCCCACCATCGGc                   >  1:1866572/1‑150 (MQ=255)
                                                                                                                               cccACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAACTTGATCACGATGACATTGATGATGTCGATGAAGAACGCCCCCACCATCGGc                   >  1:800174/1‑150 (MQ=255)
                                                                                                                                  aCGCAACTCAGAACGACGTTCTGATTGCTTGCGCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGAAGTCATTGATGATGTCGATGAAGAAGGCCCGCACCATCGGcacc                >  1:585702/1‑150 (MQ=255)
                                                                                                                                    gCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGATGAAGAACGCCCCCACCATCGGcaccac              >  2:1720978/1‑150 (MQ=255)
                                                                                                                                    gCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGATGAAGAACGCCCCCACCATCGGcaccac              >  2:746461/1‑150 (MQ=255)
                                                                                                                                       aatCAGAACGTCGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGATGAAGAACGCCCCCACCATCGGCAccaccaa           >  2:479126/3‑149 (MQ=255)
                                                                                                                                       aCTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGATGAAGAACGCCCCCACCATCGGCACCACCAg           >  2:1301673/1‑150 (MQ=255)
                                                                                                                                         tCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACa                                                       <  1:1338397/104‑1 (MQ=255)
                                                                                                                                         tCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACa                                                       >  2:1338397/1‑104 (MQ=255)
                                                                                                                                          cAGAACGTCGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGATGAAGAACGCCCCCACCATCGGCACCACCAGGaa        >  2:489300/1‑150 (MQ=255)
                                                                                                                                           aGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGATGAAGAACGCCCCCACCATCGGCACCACCAGGAAg       <  1:121519/150‑1 (MQ=255)
                                                                                                                                              acgacgTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGTTACAGCTTGATCACGATGACATTGATGATGTCGATGAAGAACGCCCCCACCATCGGCACCACCAGGAAGGCg    <  1:127607/150‑1 (MQ=255)
                                                                                                                                                gacgTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGATGAAGAACGCGCCTATCATCGGCTTCCCCCGGAAGGCGAg  <  2:550802/150‑1 (MQ=255)
                                                                                                                                                 acgTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAgg                                                                                      <  2:1805500/65‑1 (MQ=255)
                                                                                                                                                 acgTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAgg                                                                                      >  1:1805500/1‑65 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CCTGCATTGCCCGGAAATGGGCGTGCCGTTCTGGGGTGTGTGGTATGTCCTCGGCATGCTCGTGCCGACGGTACTCGGTGCCTGGTGGGGCCCCCGCATGCTGCGTTGGTAGGCTGTACCGGCAGGCCCCACGCAACTCAGAACGACGTTCTGATTGCTTGCCCTGGCTTCACAGGTTCGTGACCTCGCTTAACCCGCAACAAACCAAGGCAATGCCAGGTACAGCTTGATCACGATGACATTGATGATGTCGATGAAGAACGCCCCCACCATCGGCACCACCAGGAAGGCGAG  >  NC_002947/1133950‑1134243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑