Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,148,409 C→T 5.3% G281D (GGC→GAC)  PP_1007 ← transmembrane sensor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,148,4090CT 5.3% 222.2 / 2.1 76G281D (GGC→GAC) PP_1007transmembrane sensor
Reads supporting (aligned to +/- strand):  ref base C (34/37);  new base T (2/2);  total (36/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.45e-01

GCAGGGTTTCCAGTGGCTGGTCGAGGCGGTAGTTACCGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGCTGTCGCCGGCACTCAGGCGGATGCGCTGGTCGCGCTCCCCCTGCAGGTCGACATCGCCGCGTTGCACCC  >  NC_002947/1148260‑1148553
                                                                                                                                                     |                                                                                                                                                
gcAGGGTTTCCAGTGGCTGGTCGAGGCGGTAGTTACCGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAggcggc                                                                                                                                                  >  2:1862133/1‑150 (MQ=255)
  aGGGTTTCCAGTGGCTGGTCGAGGCGGTAGTTACCGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCg                                                                                                                                                >  2:587507/1‑150 (MQ=255)
            gTGGCTGGTCGAGGCGGTAGTTACCGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGTCGTCGATccagg                                                                                                                                      >  2:1665848/1‑150 (MQ=255)
            gTGGCTGGTCGAGGCGGTAGTTACCGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGTCGTCGATccagg                                                                                                                                      >  2:1665482/1‑150 (MQ=255)
               gCTGGTCGAGGCGGTAGTTACCGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATccaggcca                                                                                                                                   <  2:683066/150‑1 (MQ=255)
               gCTGGTCGAGGCGGTAGTTACCGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATccaggcca                                                                                                                                   >  1:683066/1‑150 (MQ=255)
                 tGGTCGAGGCGGTAGTTACCGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATccaggccagg                                                                                                                                 >  1:728195/1‑150 (MQ=255)
                   gTCGAGGCGGTAGTTACCGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATccag                                                                                                                                       <  2:1404707/142‑1 (MQ=255)
                   gTCGAGGCGGTAGTTACCGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATccag                                                                                                                                       >  1:1404707/1‑142 (MQ=255)
                   gTCGAGGCGGTAGTTACCGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTc                                                                                                                               >  2:419048/1‑150 (MQ=255)
                    tCGAGGCGGTAGTTACCGGTGACGGCAACGTGCTCCAACTGCGCATTGCAATTGATGTTCCATCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTcc                                                                                                                              >  2:1112395/1‑150 (MQ=255)
                    acGAGGCGGAAGCTACCGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTcc                                                                                                                              >  2:932156/2‑150 (MQ=255)
                     cGAGGCGGTAGTTACCGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCt                                                                                                                             <  1:932156/150‑1 (MQ=255)
                      gAGGCGGTAGTTACCGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCGAGCCCGGGTAGTAGCGCAGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCtt                                                                                                                            >  2:983956/1‑150 (MQ=255)
                      gAGGCGGTAGTTACCGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATccaggc                                                                                                                                     >  1:1020949/1‑141 (MQ=255)
                      gAGGCGGTAGTTACCGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATccaggc                                                                                                                                     <  2:1020949/141‑1 (MQ=255)
                      gAGGCGGTAGTTACCGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCtt                                                                                                                            >  2:1306489/1‑150 (MQ=255)
                      gAGGCGGTAGTTACCGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCtt                                                                                                                            <  1:1101282/150‑1 (MQ=255)
                      gAGGCGGTAGTTACCGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCtt                                                                                                                            <  1:1120750/150‑1 (MQ=255)
                        ggCGGTAGTTACCGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGTGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTtgt                                                                                                                          >  1:769256/1‑150 (MQ=255)
                        ggCGGTAGTTACCGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTtgt                                                                                                                          <  2:1619563/150‑1 (MQ=255)
                        ggCGGTAGTTACCGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTtgt                                                                                                                          <  2:600660/150‑1 (MQ=255)
                        ggCGGTAGTTACCGGTGACGACAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTtgt                                                                                                                          <  1:707734/150‑1 (MQ=255)
                         gCGGTAGTTACCGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTtgtg                                                                                                                         <  1:1355885/150‑1 (MQ=255)
                            gTAGTTACCGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCAt                                                                                                                      >  1:235384/1‑150 (MQ=255)
                            gTAGTTACCGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCAGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCAt                                                                                                                      >  2:1699297/1‑150 (MQ=255)
                               gTTACCGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTc                                                                                                                   >  2:23360/1‑150 (MQ=255)
                               gTTACCGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTc                                                                                                                   <  1:587507/150‑1 (MQ=255)
                                    cGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGggc                                                                                                              >  1:461373/1‑150 (MQ=255)
                                    cGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGggc                                                                                                              >  1:1285924/1‑150 (MQ=255)
                                      gTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGGTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGggcgc                                                                                                            >  1:1756835/1‑150 (MQ=255)
                                              aaCGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGggcgctggcgctt                                                                                                    >  1:1765372/1‑149 (MQ=255)
                                              aaCGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGgcgctggcgctg                                                                                                    <  1:1306489/150‑1 (MQ=255)
                                              aaCGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGgcgctggcgctg                                                                                                    <  2:1285924/150‑1 (MQ=255)
                                                  tGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCa                                                                                                <  1:23360/150‑1 (MQ=255)
                                                    cTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCaaa                                                                                              <  1:1208149/150‑1 (MQ=255)
                                                        aaCTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATc                                                                                                                                          >  2:1513208/1‑102 (MQ=255)
                                                        aaCTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATc                                                                                                                                          <  1:1513208/102‑1 (MQ=255)
                                                            gcgcATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCCGCCCTTAGCTCAACGGTCAGTTCTCGCACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTggg                                                                                      <  1:224599/150‑1 (MQ=255)
                                                            gcgcATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTggg                                                                                      <  1:1699297/150‑1 (MQ=255)
                                                                  tGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGTCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGa                                                                                <  1:1665482/150‑1 (MQ=255)
                                                                  tGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGTCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGa                                                                                <  1:1665848/150‑1 (MQ=255)
                                                                          atgatCCAGTCCGGGTAGTAGCGCTGTACTTCGGCCAGCACCTGGCTCAACGGCCAGTTCTCGAACACCTGGCGGATGTCGATCCAGGACCGGTCCTGGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATg                                                                        <  2:689627/150‑1 (MQ=255)
                                                                           tgatCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGc                                                                       >  1:1683512/1‑150 (MQ=255)
                                                                           tgatCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGc                                                                       <  1:1856355/150‑1 (MQ=255)
                                                                            gatCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGCt                                                                      >  2:885634/1‑150 (MQ=255)
                                                                               ccAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGCTGTc                                                                   <  2:422995/150‑1 (MQ=255)
                                                                                 ggCCCGGGTAGTAGCGCTGTACTTCGGCCAGCATTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGCTGTCGc                                                                 <  2:434499/149‑1 (MQ=255)
                                                                                  gCCCGGGTAGTAGAGCAATACATCTGGCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGCTGTCGcc                                                                >  2:1607386/1‑150 (MQ=255)
                                                                                    ccGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGAt                                                                         >  2:88404/1‑139 (MQ=255)
                                                                                    ccGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGAt                                                                         <  1:88404/139‑1 (MQ=255)
                                                                                    ccGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGCTGTCGCCgg                                                              <  1:561276/150‑1 (MQ=255)
                                                                                       ggTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGCTGTCGCCGGCAc                                                           <  2:170872/150‑1 (MQ=255)
                                                                                             agCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGCTGTCGCCGGCACTCAGGc                                                     <  2:769256/150‑1 (MQ=255)
                                                                                              gcgcTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGCTGTCGCCGGCACTCAGGCg                                                    >  1:322728/1‑150 (MQ=255)
                                                                                                  tGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGCTGTCGCCGGCACTCAGGCGGATg                                                <  1:101774/150‑1 (MQ=255)
                                                                                                  tGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGCCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGCTGTCGCCGGCACTCAGGCGGATg                                                <  1:750083/150‑1 (MQ=255)
                                                                                                    tACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGCTGTCGCCGGCACTCAGGCGGATgcg                                              >  1:1024271/1‑150 (MQ=255)
                                                                                                      cTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGCTGTCGCCGGCACTCAGGCGGATGCGCt                                            <  2:1749225/150‑1 (MQ=255)
                                                                                                      cTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGCTGTCGCCGGCACTCAGGCGGATGCGCt                                            <  2:235384/150‑1 (MQ=255)
                                                                                                       ttCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGCTGTCGCCGGCACTCAGGCGGATGCGCTg                                           >  2:177631/1‑150 (MQ=255)
                                                                                                       ttCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGCTGTCGCCGGCACTCAGGCGGATGCGCTg                                           <  2:728195/150‑1 (MQ=255)
                                                                                                         cGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGCTGTCGCCGGCACTCAGGCGGATGCGCTGGt                                         <  1:885634/150‑1 (MQ=255)
                                                                                                          ggCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGCTGTCGCCGGCACTCAGGCGGATGCGCTGGTc                                        <  2:1581856/150‑1 (MQ=255)
                                                                                                             cAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGATGCTGATGCTGTCGCCGGCACTCAGGCGGATGCGCTGGTcgcg                                     >  1:623265/1‑150 (MQ=255)
                                                                                                             cAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGCTGTCGCCGGCACTCAGGCGGATGCGCTGGTcgcg                                     >  1:622819/1‑150 (MQ=255)
                                                                                                              aGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGCTGTCGCCGGCACTCAGGCGGATGCGCTGGTCgcgc                                    <  1:177631/150‑1 (MQ=255)
                                                                                                                        tCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTggg                                                                                      >  1:1530492/1‑90 (MQ=255)
                                                                                                                        tCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTggg                                                                                      <  2:1530492/90‑1 (MQ=255)
                                                                                                                           aCGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGCTGTCGCCGGCACTCAGGCGGATGCGCTGGTCGCGCTCCCCCTGCAGGt                       <  2:461373/150‑1 (MQ=255)
                                                                                                                                      tcGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGCTGTCGCCGGCACTCAGGCGGATGCGCTGGTCGCGCTCCCCCTGCAGGTCGACATcgccg            >  1:653861/1‑150 (MQ=255)
                                                                                                                                       cGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGCTGTCGCCGGCACTCAGGCGGATGCGCTGGTCGCGCTCCCCCTGCAGGTCGACATcgccgc           <  2:1024271/150‑1 (MQ=255)
                                                                                                                                       cGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGCTGTCGCCGGCACTCAGGCGGATGCGCTGGTCGCGCTCCCCCTGCAGGTCGACATcgccgc           >  1:1099498/1‑150 (MQ=255)
                                                                                                                                       cGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGCTGTCGCCGGCACTCAGGCGGATGCGCTGGTCGCGCTCCCCCTGCAGGTCGACATcgccgc           >  1:1406873/1‑150 (MQ=255)
                                                                                                                                          acacCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGCTGTCGCCGGCACTCAGGCGGATGCGCTGGTCGCGCTCCCCCTGCAGGTCGACATCGCCGCGtt        <  1:983956/150‑1 (MQ=255)
                                                                                                                                          acacCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGCTGTCGCCGGCACTCAGGCGGATGCGCTGGTCGCGCTCCCCCTGCAGGTCGACATCGCCGCGtt        >  2:1060107/1‑150 (MQ=255)
                                                                                                                                              cAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCCGATGCTGTCGCCGGCACTCAGGCGGATGCGCTGGTCGCGCTCCCCCTGCAGGTCGACATCGCCGCGTTGCAc    >  2:1886761/1‑150 (MQ=255)
                                                                                                                                                ggcggcCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGCTGTCGCCGGCACTCAGGCGGATGCGCTGGTCGCGCTCCCCCTGCAGGTCGACATCGCCGCGTTGCAccc  >  1:1353999/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
GCAGGGTTTCCAGTGGCTGGTCGAGGCGGTAGTTACCGGTGACGGCAACGTGCTCCAACTGCGCATTGCGGTTGATGATCCAGCCCGGGTAGTAGCGCTGTACTTCGGCCAGCACTTGGCTCAACGGGCAGTTCTCGAACACCAGGCGGCCGTCGATCCAGGCCAGGTCCTTGTGCATGTCGGGGCGCTGGCGCTGGCCAAAGCCTTGGGGGCCGACGCTGATGCTGTCGCCGGCACTCAGGCGGATGCGCTGGTCGCGCTCCCCCTGCAGGTCGACATCGCCGCGTTGCACCC  >  NC_002947/1148260‑1148553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑