Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,221,043 T→G 5.6% D90A (GAC→GCC)  dctD‑II ← C4‑dicarboxylate transport transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,221,0430TG 5.6% 208.6 / 2.4 81D90A (GAC→GCC) dctD‑IIC4‑dicarboxylate transport transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (38/38);  new base G (3/2);  total (41/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

GTTCGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATGCGTTCCA  >  NC_002947/1220894‑1221187
                                                                                                                                                     |                                                                                                                                                
gTTCGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGt                                                                                                                                                  >  1:1356196/1‑150 (MQ=255)
   cGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTAAGCATGGCGCCAACGGCCATGTCgg                                                                                                                                               <  2:269444/150‑2 (MQ=255)
      ccAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGa                                                                                                                                               >  2:1649197/1‑147 (MQ=255)
      ccAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGa                                                                                                                                               <  1:1649197/147‑1 (MQ=255)
      ccAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGa                                                                                                                                               <  1:1649573/147‑1 (MQ=255)
      ccAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGa                                                                                                                                               >  2:1649573/1‑147 (MQ=255)
       cAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATc                                                                                                                                           <  1:480832/150‑1 (MQ=255)
              cggcgCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGGCGATATCGACATgg                                                                                                                                    <  2:867932/150‑1 (MQ=255)
                  gcAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAg                                                                                                                                >  2:460193/1‑150 (MQ=255)
                  gcAGGACCACCACTTCGCGTAACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGAGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGAGCCGTTACGCATGGCACCAACGGCAATGTCGATATAGCAATAGCCAg                                                                                                                                >  2:7091/1‑150 (MQ=255)
                    aGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTg                                                                                                                              <  1:1783386/150‑1 (MQ=255)
                      ggCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGAt                                                                                                                            <  1:1217782/150‑1 (MQ=255)
                       gCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATc                                                                                                                           <  1:1393770/150‑1 (MQ=255)
                          accacTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGc                                                                                                                        >  2:1855197/1‑150 (MQ=255)
                            cacTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGcac                                                                                                                      >  1:421694/1‑150 (MQ=255)
                               ttCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGcaccac                                                                                                                   <  1:460193/150‑1 (MQ=255)
                               ttCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGcaccac                                                                                                                   >  2:1229457/1‑150 (MQ=255)
                               ttCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGcaccac                                                                                                                   <  1:1229457/150‑1 (MQ=255)
                               ttCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGcaccac                                                                                                                   <  2:813403/150‑1 (MQ=255)
                                  gcgTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGAGGGAGAACGGTTTTTCCATGAAGTAGTAGGCGCGGTTACGCATGGCGCCAACGGCCATGTCGAGATCACCATGGCCAGTGATCAGCACCACCgg                                                                                                                >  2:517028/1‑150 (MQ=255)
                                     tGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAg                                                                                                             >  2:178905/1‑150 (MQ=255)
                                          gcccgcccTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCGCGGGGGGGAACGGTTTTTCCATCAAGTCGTAGGAACCGAACCGCCTGGCGCGCATTTTGATATTTATTTGGCCATGGCCGGGGATCAGCACCACCGGCAGGCTGc                                                                                                        >  2:880259/1‑150 (MQ=255)
                                           cccGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCg                                                                                                       >  1:1623974/1‑150 (MQ=255)
                                           cccGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCg                                                                                                       <  2:381759/150‑1 (MQ=255)
                                             cgcgCTGTTCCAGCGCGCGGCATACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGt                                                                                                     >  2:499591/1‑150 (MQ=255)
                                              gcgcTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTc                                                                                                    >  1:1490818/1‑150 (MQ=255)
                                                cgatcttcAGCGCGCCG‑GTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATGGACCAGGCCGGTGAGCAGGACCACCGGCAGGCTGCGGTCACg                                                                                                 >  1:1373690/8‑150 (MQ=255)
                                                 cTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACg                                                                                                 >  2:458418/1‑150 (MQ=255)
                                                  tGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACgg                                                                                                <  2:1690606/150‑1 (MQ=255)
                                                    ttCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGGTATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACgg                                                                                                >  2:978366/1‑148 (MQ=255)
                                                    ttCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACgg                                                                                                <  1:978366/148‑1 (MQ=255)
                                                    ttCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGc                                                                                              >  2:1346384/1‑150 (MQ=255)
                                                     tCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGCCACGGGcc                                                                                             <  2:1623974/150‑1 (MQ=255)
                                                           gcgcgGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAgg                                                                                                            >  1:1467942/1‑129 (MQ=255)
                                                           gcgcgGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAgg                                                                                                            <  2:1467942/129‑1 (MQ=255)
                                                             gcggcgTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCg                                                                                     <  1:1855197/150‑1 (MQ=255)
                                                              cggcgTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTGGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCACGGCCAGTGATCAGCACCACCGGCGGGCTGCGGTCTCGTGCCTTGATGCGa                                                                                    <  2:1552159/150‑1 (MQ=255)
                                                                gcgTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGAtt                                                                                  <  1:1350699/150‑1 (MQ=255)
                                                                     ccACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGagca                                                                             >  1:1650391/1‑150 (MQ=255)
                                                                         gTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTc                                                                         >  1:1539091/1‑150 (MQ=255)
                                                                          tCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTcc                                                                        >  2:1622417/1‑150 (MQ=255)
                                                                            gACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAg                                                                      <  2:1539091/150‑1 (MQ=255)
                                                                            gACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAg                                                                      <  2:1490818/150‑1 (MQ=255)
                                                                             aCCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGAtt                                                                                  >  1:1189445/1‑137 (MQ=255)
                                                                             aCCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGAtt                                                                                  <  2:1189445/137‑1 (MQ=255)
                                                                              ccAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGc                                                                    <  2:1456317/150‑1 (MQ=255)
                                                                                      tCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATa                                                            >  2:1085149/1‑150 (MQ=255)
                                                                                      tCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATa                                                            >  1:1516501/1‑150 (MQ=255)
                                                                                        gggggAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATAcc                                                          >  1:714702/1‑150 (MQ=255)
                                                                                        gggggAGAACGGTTTTTCCATGAAGCCGTACGCTCTTTTACTCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATAcc                                                          <  2:1589703/150‑1 (MQ=255)
                                                                                         ggggAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATAccc                                                         <  2:1650391/150‑1 (MQ=255)
                                                                                              gaACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAg                                                    >  1:1637988/1‑150 (MQ=255)
                                                                                              gaACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAg                                                    >  1:325882/1‑150 (MQ=255)
                                                                                               aaCGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATgg                                                                                                                                    <  1:737764/69‑1 (MQ=255)
                                                                                               aaCGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATgg                                                                                                                                    >  2:737764/1‑69 (MQ=255)
                                                                                               aaCGGTTTTTCCATGAAGGCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAgg                                                   <  1:753477/150‑1 (MQ=255)
                                                                                                          cATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCt                                        >  2:379586/1‑150 (MQ=255)
                                                                                                          cATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCt                                        <  1:458418/150‑1 (MQ=255)
                                                                                                            tGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTga                                      <  1:993215/150‑1 (MQ=255)
                                                                                                             gAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCCCCAGGCCATCAATACCCGGCAGGCGGCTACCGCTgac                                     >  2:715684/1‑150 (MQ=255)
                                                                                                                   gTAGGCGCCGTTACGCATGGCGCCAGCGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAAt                               <  2:1885221/150‑1 (MQ=255)
                                                                                                                   gTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGTGGCGATTGa                                                                                >  1:1755900/1‑101 (MQ=255)
                                                                                                                   gTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGa                                                                                <  2:1755900/101‑1 (MQ=255)
                                                                                                                      ggCGCCGTTACGCATGGCGCCAACGGCCCTGTCGATATCGCCATGGCCAGTGAGCAGCCCTACCGGCTGGCTGCGCTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCCCTGACGACAATGcc                            >  1:1239398/1‑150 (MQ=255)
                                                                                                                       gcgcCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCg                           <  2:714702/150‑1 (MQ=255)
                                                                                                                       gcgcCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCg                           >  2:1183291/1‑150 (MQ=255)
                                                                                                                             ttACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCaaaa                     >  1:382447/1‑150 (MQ=255)
                                                                                                                             ttACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCaaaa                     >  1:1832868/1‑150 (MQ=255)
                                                                                                                                  cATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAAtcgtc                <  1:171264/150‑1 (MQ=255)
                                                                                                                                  cATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAAtcgtc                <  1:1085149/150‑1 (MQ=255)
                                                                                                                                  cATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAAtcgtc                <  2:382447/150‑1 (MQ=255)
                                                                                                                                  cATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAAtcgtc                <  2:1832868/150‑1 (MQ=255)
                                                                                                                                  cATGGCGCCAACGGCCATGGCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTAACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAAtcgtc                <  1:573237/150‑1 (MQ=255)
                                                                                                                                    gggCCCCACCGGCGATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGACAGGCCAGTATCGCTGACGACAATGCCGGCAAAATCGTCGc              <  1:1401248/149‑1 (MQ=255)
                                                                                                                                        gcCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGAt          <  1:1826293/150‑1 (MQ=255)
                                                                                                                                          cAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATGc        >  1:838609/1‑150 (MQ=255)
                                                                                                                                            ctggCCATGGCGATATCGACATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATGCGt      >  2:444795/3‑150 (MQ=255)
                                                                                                                                             tggCCATGGCGATATCGACATGGCCGGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATGCGtt     >  1:242190/2‑150 (MQ=255)
                                                                                                                                             tggCCATGGCGATATCGACATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATGCGtt     >  1:942826/2‑150 (MQ=255)
                                                                                                                                                ccATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATGCGTTCCa  >  2:562893/1‑150 (MQ=255)
                                                                                                                                                ccATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATGCGTTCCa  <  2:325882/150‑1 (MQ=255)
                                                                                                                                                ccATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATGCGTTCCa  >  1:1478799/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
GTTCGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATGCGTTCCA  >  NC_002947/1220894‑1221187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑