Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,261,563 C→G 5.1% E360Q (GAA→CAA)  PP_1104 ← AotO‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,261,5630CG 5.1% 249.4 / 4.3 79E360Q (GAA→CAA) PP_1104AotO‑related protein
Reads supporting (aligned to +/- strand):  ref base C (37/38);  new base G (2/2);  total (39/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.46e-01

GCAAACCAGGCATTGGCGAGGTCGGTAGCAGCAGGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTGGGTCGATGACTTCGGCGATCAGTTGGCCGGCCTCCAGG  >  NC_002947/1261419‑1261711
                                                                                                                                                 |                                                                                                                                                    
gCAAACCAGGCATTGGCGAGGTCGGTAGCAGCAGGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCGAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCgg                                                                                                                                                 >  1:1037049/1‑150 (MQ=255)
        ggCATTGGCGAGGTCGGTAACTTCAGGCATGAGGGCAGGCGGTCCTTCGAAGGCCGTGCTTCTACAGACCACTTGACGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATgg                                                                                                                                         <  1:1038542/150‑1 (MQ=255)
         gCATTGGCGAGGTCGGTAGCAGCAGGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GACGCTGCGGCTTACCTGGTCGCCGGCCACATggg                                                                                                                                        >  2:144903/1‑150 (MQ=255)
         gCATTGGCGAGGTCGGTAGCAGCAGGCATGAGGGCAGGCGGTCCTTCGAAGGCTGGGCTTCTCCAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATggg                                                                                                                                        <  2:1538748/150‑1 (MQ=255)
           aTTGGCGAGGTCGGTAGCAGCAGGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATGAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCg                                                                                                                                      >  2:1038328/1‑150 (MQ=255)
             gggCGAGGTCGGTAGGCGCACGCATGAGGGGCGGCGGTCCGTGGACCAGCCCGCCGTTACAGAGCACTTGCCGGCTGCAATGGTCTTACCGGATCAATTGGCCAACAGGGA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGAt                                                                                                                                    <  2:990513/149‑1 (MQ=255)
               gCGAGGTCGGTAGCAGCAGGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGa                                                                                                                                  >  2:520558/1‑150 (MQ=255)
               gCGAGGTCGGTAGCAGCAGGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGa                                                                                                                                  <  1:457539/150‑1 (MQ=255)
                 gAGGTCGGTAGCAGCAGGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGAcc                                                                                                                                >  1:583901/1‑150 (MQ=255)
                         ttgcagcaGGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCCCATGGGCGATGACCATGCCAGc                                                                                                                        >  2:903137/3‑150 (MQ=255)
                           gcagcaGGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCgg                                                                                                                      >  2:1396560/1‑150 (MQ=255)
                             ggcagGCGTGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTg                                                                                                                    <  2:1239369/149‑1 (MQ=255)
                             agcagGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTg                                                                                                                    <  2:1707640/150‑1 (MQ=255)
                             agcagGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTg                                                                                                                    >  1:186732/1‑150 (MQ=255)
                              gcagGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTgg                                                                                                                   <  1:1723414/150‑1 (MQ=255)
                              gcagGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTgg                                                                                                                   <  1:520558/150‑1 (MQ=255)
                               cagGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGc                                                                                                                  >  2:1280535/1‑150 (MQ=255)
                                  gCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCAt                                                                                                               <  1:266531/150‑1 (MQ=255)
                                   cATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATg                                                                                                              >  2:1602428/1‑150 (MQ=255)
                                          gCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGa                                                                                                       <  1:1038328/150‑1 (MQ=255)
                                          gCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGACGACCATGCCAGCGGTGGCCATGCGCCGGa                                                                                                       <  2:1369943/150‑1 (MQ=255)
                                             ggCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCCGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGc                                                                                                    <  1:256642/150‑1 (MQ=255)
                                              gCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATgcgc                                                                                                           >  2:1485135/1‑142 (MQ=255)
                                              gCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATgcgc                                                                                                           <  1:1485135/142‑1 (MQ=255)
                                              gCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGACATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCt                                                                                                   <  1:1318468/150‑1 (MQ=255)
                                                  tCCGTCGAAGGCGGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTCCCGGGTCAATTGGCCAGCAGGTA‑GCCGGTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCTAGCGGTGGCCATGCGCCGGACGCTAcgc                                                                                               <  2:244857/150‑1 (MQ=255)
                                                       cGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATa                                                                                          <  2:1567288/150‑1 (MQ=255)
                                                        gAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATAc                                                                                         >  2:1457625/1‑150 (MQ=255)
                                                            gCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATAcagca                                                                                     >  2:70734/1‑150 (MQ=255)
                                                                   ttCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGGTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCGTACAGCAGCCCCgg                                                                              >  2:590968/1‑150 (MQ=255)
                                                                   ttCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACGGCCGCCCCgg                                                                              >  2:687272/1‑150 (MQ=255)
                                                                   ttCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCgg                                                                              >  2:875645/1‑150 (MQ=255)
                                                                      tACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCggctg                                                                           >  1:304666/1‑150 (MQ=255)
                                                                       aCAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCggctgg                                                                          <  2:186732/150‑1 (MQ=255)
                                                                       aCAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCggctgg                                                                          >  2:373729/1‑150 (MQ=255)
                                                                       aCAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCAATTTTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCggctgg                                                                          <  1:33931/150‑1 (MQ=255)
                                                                            ccACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGtt                                                                     <  1:1715340/150‑1 (MQ=255)
                                                                             cACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTg                                                                    >  1:1280870/1‑150 (MQ=255)
                                                                                ttGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTgcgc                                                                 >  2:257924/1‑150 (MQ=255)
                                                                                 tGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCa                                                                <  1:373729/150‑1 (MQ=255)
                                                                                  gCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAg                                                               <  2:1280870/150‑1 (MQ=255)
                                                                                      gCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAggggg                                                           <  2:814593/150‑1 (MQ=255)
                                                                                      gCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAggggg                                                           <  1:409066/150‑1 (MQ=255)
                                                                                      gCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCGGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAggggg                                                           >  2:673886/1‑150 (MQ=255)
                                                                                          cAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGa                                                       >  1:1626938/1‑150 (MQ=255)
                                                                                          cAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGa                                                       >  1:498570/1‑150 (MQ=255)
                                                                                          cAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGAACATGCCAGCGGTGGCAACGCGCCGGACGCGACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGa                                                       <  1:1180372/150‑1 (MQ=255)
                                                                                           aaTGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGAt                                                                                                                                    >  1:142055/1‑72 (MQ=255)
                                                                                           aaTGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGAt                                                                                                                                    <  2:142055/72‑1 (MQ=255)
                                                                                           aaTGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCAAGAGCGTCGTGTCCGGCTGGCAGTTGCGCAGGGGGGTGAc                                                      >  2:152571/1‑150 (MQ=255)
                                                                                              ggTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCg                                                   <  2:304666/150‑1 (MQ=255)
                                                                                                tttgACCGGATGAATTGGGCAGCGGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGCCCTTGCCAGCGGTGGCCATGCGCCAGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGt                                                 <  2:1004376/150‑1 (MQ=255)
                                                                                                   tACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCAc                                              <  1:1236243/150‑1 (MQ=255)
                                                                                                     ccGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTg                                            >  2:357617/1‑150 (MQ=255)
                                                                                                      cGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGa                                           <  1:1457625/150‑1 (MQ=255)
                                                                                                      cGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGa                                           <  2:583901/150‑1 (MQ=255)
                                                                                                        taTCAATTGGCCAACAGGTA‑GCCGCTGCGCCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGt                                         <  1:777866/149‑1 (MQ=255)
                                                                                                         aTCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTg                                        >  1:711389/1‑150 (MQ=255)
                                                                                                             aTTGGCCAACAGGTA‑GCCGCTGCGGTATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCAATGAGTGGGTc                                    >  1:546591/1‑150 (MQ=255)
                                                                                                               tGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTGGGTCGa                                  <  1:379773/150‑1 (MQ=255)
                                                                                                                  ccAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTGGGTCGATGa                               >  1:991297/1‑150 (MQ=255)
                                                                                                                   gaaCAGGTATGCCGCAGCGGC‑TACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTGGGTCGATGAc                              >  2:805609/2‑150 (MQ=255)
                                                                                                                   cAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGCGGGTCGATGAc                              >  2:1914488/1‑150 (MQ=255)
                                                                                                                     aCAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTGGGTCGATGACtt                            <  2:1626938/150‑1 (MQ=255)
                                                                                                                      cAGGTATGCCGCAGCGGC‑TACCTGTTGGCCGGCCACATGGGCGATGACCATGCCAGCGGTg                                                                                                                    >  1:1677621/1‑61 (MQ=17)
                                                                                                                      cAGGTATGCCGCAGCGGC‑TACCTGTTGGCCGGCCACATGGGCGATGACCATGCCAGCGGTg                                                                                                                    <  2:1677621/61‑1 (MQ=17)
                                                                                                                      cAGGTATGCCGCAGCGGC‑TACCTGTTGGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACt                                             <  1:970133/132‑1 (MQ=255)
                                                                                                                      cAGGTATGCCGCAGCGGC‑TACCTGTTGGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACt                                             >  2:970133/1‑132 (MQ=255)
                                                                                                                        ggTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTGGGTCGATGACTTCgg                         <  1:257924/150‑1 (MQ=255)
                                                                                                                             gCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTGGGTCGATGACTTCGGCGAt                     <  2:1898058/150‑1 (MQ=255)
                                                                                                                               cGCNGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTTAGTGGGTCGATGACTTCGGCGATCa                   >  2:1187220/1‑150 (MQ=255)
                                                                                                                                        aTACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTGGGTCGATGACTTCGGCGATCAGTTggccgg          >  2:10526/1‑150 (MQ=255)
                                                                                                                                         tACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTGGGTCGATGACTTCGGCGATCAGTTggccggc         <  1:10526/150‑1 (MQ=255)
                                                                                                                                          aCCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTGGGTCGATGACTTCGGCGATCAGTTggccg           >  2:811985/1‑147 (MQ=255)
                                                                                                                                          aCCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTGGGTCGATGACTTCGGCGATCAGTTggccg           <  1:811985/147‑1 (MQ=255)
                                                                                                                                             tGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTGGGTCGATGACTTCGGCGATCAGTTGGCCGGCCTcc     >  2:662265/1‑150 (MQ=255)
                                                                                                                                              gTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTGGGTCGATGACTTCGGCGATCAGTTGGCCGGCCTCCa    >  1:1259295/1‑150 (MQ=255)
                                                                                                                                               ttCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCGTACAGCAGCTCCGGCTGGCTGTGGCGCAGGGGGGGGACTCGGTCACTGAGTGGGTCGATGACTTCGGCGAGCAGTTGGCCGGCCTCCAg   >  2:389464/1‑150 (MQ=255)
                                                                                                                                                tCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTGGGTCGATGCCTTCGGCGATCAGTTGGCCGGCCTCCAgg  <  2:546591/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GCAAACCAGGCATTGGCGAGGTCGGTAGCAGCAGGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTGGGTCGATGACTTCGGCGATCAGTTGGCCGGCCTCCAGG  >  NC_002947/1261419‑1261711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑